• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.37-37
• /
• 2020

홍띠(Imperata cylindrica 'Rubra') 식물자원의 생장점 부위를 기내 배양하여 기내 식물체 재분화와 재분화식물체의 유전적 안정성을 검토하였다. 기내배양은 26±2 ℃, 25 μmol/m²/s, 14h/10h (day/night) 광조건 하의 배양실에서, MS (Murashige and Skoog, 1962) 기본배지에 생장조절물질을 첨가하여 조직절편체로부터 식물체를 유도하였다. 캘러스는 MS기본배지에 0.1 mg/L의 2,4-D와 2 mg/L의 BA를 혼용처리하여 생장점 부위로부터 유도하였다. 캘러스 증식은 MS기본배지에 0.1 mg/L의 2,4-D를 첨가한 배지에서, 이들 캘러스로부터 신초 재분화는 0.01 mg/L의 NAA 및 2 mg/L의 BA를 첨가한 배지에서 유도하였다. 다경줄기 형성(multiple shooting) 후 MS배지에서 4주 동안 배양한 재분화식물체는 멸균한 상토(버미큘라이트)를 포함한 배양병에서 7주간 배양한 다음 점차적으로 배양병 뚜껑을 개방(1/10 정도 1차 개방 1주일, 3/10 정도 2차 개방 2주일)하여 직경 6 cm의 컵포트에 이식하여 활착시켰다. 재분화식물체는 붉은색이 사라지고 녹색을 나타내었으며, 일부개체에서만 잎의 일부분만 붉은색을 나타냈다. 이는 생장점 주변조직에서 유래한 재분화체가 우세함으로써 생장점보다는 생장점 주변의 조직을 구성하는 녹색층에서 주로 식물체가 재생되는 것으로 판단된다. 이에 따라 홍띠 대조구식물체 8개체, 활착한 녹색 재분화 식물체 20개체(실내 재배중인 순화체 10개체, 2020년 6개월간 포장에서 재배중인 순화체 10개체)를 대상으로 ISSR분석을 실시하여 재분화식물체의 유전적 안정성을 검토하였다. 향후 조직학적 측면에서 신초재분화의 기원에 대한 검토가 필요하다고 사료된다.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.20
• /
• pp.1-26
• /
• 1975

In order to provide basic information for the application of tissue culture to a rice breeding program. experiments were carried out on the callus formation from seedings and anthers and organ differentiation from the callus of rice varieties and their hybrids. It appeared that in general the callus tissue was more easily induced in japonicas than in indicas with significant varietal differences. A highly significant positive correlation (r=0.896$^{**}$) was obtained between the fresh weight of callus induced in NAA medium and in 2, 4-D medium in the same variety. Callus formed on the medium with 2, 4-D was more friable than that formed on the medium with NAA. The callus formation from seedlings was better than from anthers in upland rice varieties. The easiness of the callus formation appeared to be a dominant character in crosses of rice varieties. Varietal difference was also noticed in the organ differentiation from the callus and the root differentiation seemed to be easier than the shoot differentiation. Most of the plants derived from the cal1us were albinos and the frequency of occurrence of albinos was greater in callus of seedlings than in that of anthers. Greater amount of callus tissue was formed from anthers of $F_1$ plants than anyone of their parents in remote-crosses but it was clear in close-crosses. A highly significant positive correlation (r=0.504$^{**}$) was found between the callus formation from seedlings and from anthers.

• The Korean Journal of Mycology
• /
• v.41 no.3
• /
• pp.192-196
• /
• 2013

Fungal development is largely affected by many environmental factors. In a model filamentous fungus Aspergillus nidulans, asexual development is promoted by exposure of light, presence of salt and non-fermentable sugars. In other hand, sexual development is largely induced by absence of light, fermentable sugars and hypoxic condition. Also, some important genes including veA and nsdD play positive roles in activating sexual development. Here, we reported that the effect of high concentration of $CO_2$ on developmental decision in A. nidulans. When wild-type $veA^+$ strain was cultured in normal condition, sexual and asexual development occurred in balanced manner. However, high concentration of $CO_2$ (~5%) strongly activated sexual development and inhibited asexual development. Furthermore, this $CO_2$ effect was controlled by the veA or nsdD gene. High $CO_2$ culture of $veA^-$ or $nsdD^-$ mutant didn't activate sexual development, suggesting that the activation of sexual development induced by high $CO_2$ cannot overcome the genetic requirement of sexual development such as veA or nsdD. Since 5% $CO_2$ is an important condition for human pathogenic fungi for surviving and adapting in human body, this developmental pattern of A. nidulans affected by $CO_2$ concentration may provide interesting clues for comparative study with human fungal pathogens including Aspergillus fumigatus.

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.271-271
• /
• 2004

본 실험은 TGF-a를 처리하여 분화가 유도된 인간배아 줄기세포를 파킨슨 동물모델에 이식하여 숙주세포에서의 생존 및 이식효과를 검토하고자 실시하였다. TGF-a로 분화된 세포의 이식효과를 판정하고자 배양시 TGF-a처리군과 처리하지 않은 군으로 나누어 분화를 유도한 인간배아 줄기세포를 hoechst33342로 표지 하여 병변 유발과 동일한 방법으로 동측 선조체내에 4×10⁴개/2ul가 되도록 이식하고(이식 위치: AP 0.7, ML 2.0, DV3.4) 이식 후 2, 4주에서 행동학적 변화를 관찰하고 4주에 동물을 희생시켜 4% PFA를 이용하여 뇌 조직을 고정하고 뇌 조직은 40㎛ 두께로 동결 절편을 만들어 면역조직화학염색을 시행하여 신경세포로의 분화 및 TH 발현 여부를 관찰하였고 분화의 표지물질로 nestin, NF200, GFAP, TH를 사용하여 형태학적 변화를 관찰하였다. (중략)

• Journal of the Korean Institute of Intelligent Systems
• /
• v.11 no.1
• /
• pp.70-75
• /
• 2001

종의 분화는 생명체의 다양성을 유지하며, 좀더 환경에 적합한 생명체를 탄생시킨다. 그래서, 자연계의 진화에 모방을 둔 진화 알고리즘은 주어진 환경에 적응하기 위해 다양성을 유지해야 한다. 본 논문에서는 이러한 종의 분화 개념을 도입한다. 개체군의 각 개체들이 돌연변이를 통하여 자손을 생성하고, 그 중 일부가 분화하여 다음 세대의 개체를 이룬다. 각 개체들은 돌연변이에 의해 결정되는 일정한 해밍 공간 내외를 탐색공간으로 하고, 분화를 통하여 유효한 탐색공간을 점차 넓혀 탐색공간 전체에 대한 효율적인 탐색을 수행한다. 돌연변이를 통한 진환 방법으로 진화 하드웨어에 적용할 경우 내부구조의 변경이 적어 빠른 탐색효과를 가질 수 있다. 제안된 알고리즘을 2개의 최적화 문제에 적용하여 그 유용성을 확인한다.

• Korean Journal of School Psychology
• /
• v.17 no.1
• /
• pp.65-86
• /
• 2020

The purpose of this study was to examine whether self-differentiation mediates the relationship between adolescents' family functioning and self-esteem and to examine whether peer support and family conflicts had moderated mediating effects. For this purpose, 522 students from four high schools in Gyeonggi province were surveyed about their family functioning, self-differentiation, self-esteem, and peer support. The results were analyzed using SPSS 21.0 and PROCESS. The mediating, modulating, and moderated mediating effects were analyzed. The results of this study were as follows. First, correlation analysis showed that family functioning, self-differentiation, self-esteem, and peer support were significantly positively correlated. Second, the moderated mediating effects of peer support were as follows. First, self-differentiation had a partial mediating effect in the relationship between family functioning and self-esteem such that family functioning and self-differentiation were positively correlated and self-differentiation and self-esteem were positively correlated. Second, peer support had a moderating effect in the relationship between family functioning and self-differentiation that promoted self-differentiation. Thus, peer support affected family functioning and self-differentiation. Third, verifying the moderated mediating model of peer support by combining the mediation model of self-differentiation and the moderation model of peer support identified above showed that self-differentiation mediated the relationship between family functioning and self-esteem. Thus, family support affected self-differentiation and self-differentiation affected self-esteem. The significance of this study was as follows. First, it showed that self-differentiation significantly mediated the relationship between family functioning and self-esteem, clarifying the relationship between family functioning and self-esteem. Second, it showed that peer support moderated the relationship between family functioning and self-differentiation, identifying a mechanism that promotes self-differentiation. Third, it showed the mediation of self-differentiation of the relationship between family functioning and self-esteem depended on peer support.

• Korean Journal of Plant Resources
• /
• v.36 no.5
• /
• pp.496-507
• /
• 2023

The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica 'Rubra', Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

• Korean Journal of Mineralogy and Petrology
• /
• v.33 no.3
• /
• pp.293-305
• /
• 2020

An eruption of Taal Volcano in the Philippines began on January 12, 2020. The Philippine Institute of Volcanology and Seismology (PHIVOLCS) subsequently issued an Alert Level 4, indicating that "a hazardous explosive eruption is possible within hours to days." It was a phreatic eruption and phreatomagmatic eruption from the main crater that spewed ashes to Calabarzon, Metro Manila, some parts of Central Luzon, and Pangasinan in Ilocos Region, resulting in the suspension of classes, work schedules, and flights. By January 26, 2020, PHIVOLCS observed inconsistent, but decreasing volcanic activity in Taal, prompting the agency to downgrade its warning to Alert Level 3. After February 14, Alert status was set to Level 2 because of overall decreasing trend of volcanic activities, but it does not mean that the threat of an eruption has disappeared. In addition, the Alert Level can be raised to Alert Level 3 if there is a symptom of increasing unrest at any time.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.1
• /
• pp.7-11
• /
• 1995

Protoplasts were isolated from hypocotyl tissues of 5-day-old Brassica oleracea var capitata Green Challenger seedlings. Several media were used for protoplast culture and shoot regeneration. The shoot-regeneration rapacity of protoplast derived callus depended on the initial culture medium. Protoplasts were cultured in liquid medium (B5 medium supplemented with CaCl2, 2H2O 600mg/L, g1ucose 20g/L, D-mannito1 70g/L, NAA lmg/L, BA lmg/L, 2.4-D 0.25 mg/L)at 27$^{\circ}C$ under the dark After 5 to 10 days, cultlues were diluted with medium with a reduced osmotic stabilizer and then transferred to illuminated conditions. The culture medium was changed with the fresh medium at 7- to 10-day-intervals until the formation of microcallus. Hypocotyl protoplast-derived callus proliferated when transferred to MS medium supplemented with NAA lmg/L, BA 1mg/L and GA$_3$ 0.02mg/L. Upon transfer to MS basal medium without growth regulators, roots were produced. In an attempt to increase the regeneration frequency, 10g/L polyvinylpyrrolidone was added to the regeneration medium, but the shoot regeneration was mot improved. The regenerated whole plants were acclimated in a sterized soilless mixture(vermiculite 2;perlite 2;peat moss1) in a culture room.

• Journal of Animal Science and Technology
• /
• v.49 no.1
• /
• pp.25-32
• /
• 2007

The current study was undertaken to determine the effect of conjugated linoleic acid(CLA) isomers, cis-9, cis-11(c9c11), cis-9, trans-11(c9t11), trans-9, trans-11(t9t11), trans-10, cis-12(t10c12) on differentiation of pig preadipocytes and myogenic satellite cells during culture. Cells were isolated from new born pigs. The t10c12 isomer decreased differentiation of pig preadipocytes(92%), but not that of myogenic cells. The t9t11 isomer decreased differentiation of preadipocytes(14%) and increased that of myogenic cells (26%). No other CLA isomers affected differentiation of preadipocytes or myogenic cells. The effects of CLA on proliferation of preadipocytes and myogenic cells were small, compared to the effects on differentiation. These results suggest that CLA isomers have different effects on differentiaton of pig preadipocytes and myogenic cells.