• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.127-135
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• 2019

The purpose of this study was to investigate suitable parts for callus induction and optimal concentrations of growth regulators, contained in the medium affecting shoot and rooting for in vitro mass production of Haworthia truncata. Leaves and flower bud showed 100% callus formation rate at NAA $1{\sim}2mgL^{-1}$ treatment, and NAA $1mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment. The flower stalk showed 75% callus formation rate, at NAA $2mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment in H. truncata. While the rate of callus formation was high in leaves and flower bud, leaves were the most efficient in obtaining most culture parts. Shoot induction rate from callus was highest, at NAA $0.1mgL^{-1}$ treatment in H. truncata. Additionally, the number of shoots formation was 66.3 shoots high, in NAA $1mgL^{-1}$ + BA $0.1mgL^{-1}$ treatment in H. truncata. In the case of acclimatization of regenerated plant, growth characteristics did not show significant difference (95%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate, considering plant height and appearance preference of H. truncata. It was established that optimization of culture condition, was responsible for mass propagation in vitro cultures of H. truncata.

• Radiation Oncology Journal
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• v.24 no.2
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• pp.110-115
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• 2006

Purpose: This study was performed to determine the prognostic factors influencing relapse pattern, overall and disease-free survival in patients treated with postoperative radiotherapy for endometrial carcinoma. Materials and Methods: The records of 54 patients with endometrial adenocarcinoma treated postoperative radiotherapy at Pusan National University Hospital between April 1992 and May 2003 were reviewed retrospectively. Median age of the patients was 55 (range $35{\sim}76$). The distribution by surgical FIGO stages were 63.0% for 0Stage I, 14.8% for Stage II, 22.2% for Stage III. All patients received postoperative external radiotherapy up to $41.4{\sim}54Gy$ (median: 50.4 Gy). Additional Intravaginal brachytherapy was app led to 20 patients (37.0% of all). Median follow-up time was 35 months ($5{\sim}115$ months). Significant factors of this study: histologic grade, Iymphovascular space invasion and myometrial invasion depth were scored (GLM score) and analyzed. Survival analysis was peformed using Kaplan-Meier method. The log-rank test was used for univariate analysis and the Cox regression model for multivariate analysis. Results: 5-year overall and disease-free survival rates were 87.7% and 871%, respectively. Prognostic factors related with overall and disease-free survival were histologic grade, Iymphovascular space invasion and myometrial invasion according to the univariate analysis. According to the multivariate analysis, Iymphovascular space invasion was associated with decreased disease-free survival. GLM score was a meaningful factor affecting overall and disease-free survival (p=0.0090, p=0.0073, respectively) and distant recurrence (p=0.0132), which was the sum of points of histologic grade, Iymphovascular space Invasion and myometrial invasion. Total failure rate was 11% with 6 patients. Relapse sites were 2 para-aortic Iymph nodes, 2 lungs, a supraclavicular Iymph node and a vagina. Conclusion: The prognosos in patients with endometrial carcinoma treated by postoperative radiotherapy was closely related with surgical histopathology. If further explorations confirm the system of prognostic factors in endometrial carcinoma, it will help us to predict the progression pattern and to manage.

• The Korean Journal of Food And Nutrition
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• v.28 no.4
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• pp.650-657
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• 2015

This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.233-239
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• 1995

The effects of media, growth regulators, low-temperature treatments, culture temperature and light were investigated to improve the callus induction and growth in the anther culture of Comus officinalis Sieb. et Zucco. The frequency of callus induction was more effective on WPM medium than MS medium, and it was highest as 54% in WPM medium supplemented with Img/L NAA. Callus growth was stimulated on MS medium supplemented with 2mg/L NAA. Effect of temperature and light on the callus induction and growth was highest as 62% in the treatment for 16/8 hrs. (light/dark) at $25^{\circ}C$ Ef­fect of low - temperature treatment on callus induction was highest as 19. 5% in the treatment for 36 hrs. at $4^{\circ}C$. For organization, green cells and rootings were promoted on MS medium supplemented with O. 5mg/L 2,4-D and 1 mg/L kinetin. The prevention of callus browning was effective on the medium con­taining $3{\sim}5mg/L$ ABA or 5mg/L $AgNO_3$. The supplement of ABA or $AgNO_3$,were maintained callus ac­tivity for 4-5 weeks and they were promoted the development of green cells.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.327-331
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• 2006

This study was carried out to determine the economic injury level of the rice leaffolder,Cnaphalocrocis medinalis G.. The damage aspects of rice plant (at tilling stage) by leaf folders at different larval density per plant were studied in pot experiment (24 cm in diameter, 18 cm in height). One leaf folder consumed 6-7 leaves during larval stage. The damage by leaf folders was simulated by cutting off 0, 10, 30, 50, 70, and 90% of leaves before and after heading stage July 15th (at panicle initiation stage) and August 15th (at milk stage), respectively. When leaves were cut before the heading of rice, the linear relationships between the leaf cutting rate (X) and each factors of yield (Y) were as following; for grain maturity it was Y = -9.379X + 83.630 ($R^{2}=0.493$),\;Y = 0.139X + 0.490 ($R^{2}=0.925$) for yield, and Y = -4.880X + 81.116 ($R^{2}=0.665$) for head rice. When leaves were cut after the heading of rice, it was Y = -23.0l4X + 83.589 ($R{2}=0.915$), for grain maturity, Y=0.141X + 3.466 ($R^{2}=0.842$) for yield, and Y = -13.795X + 81.964 ($R^{2}=0.898$) for head rice. We found that when leaf cutting after the heading stage caused more damage than before the heading in terms of yield and yield components. Based on theses results the economic threshold level was estimated to be 30% and 7% leaf loss before and after heading stage.

• Journal of Life Science
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• v.21 no.6
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• pp.783-787
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• 2011

The effects of thaumatin isolated from katemfe fruit of Thaumatococcus daiellii Benth on 3T3-L1 preadipocyte differentiation was investigated in vitro. 3T3-L1 adipocytes were treated with various concentrations of thaumatin ranging in 0-5 ${\mu}M$. Thaumatin reduced fat accumulation in differentiated 3T3-L1 adipocytes in a dose-dependent manner. 3T3-L1 cell proliferation was 97.0 and 88.3% at 1 and 3 ${\mu}M$ after 8 days of thaumatin treatment, respectively. Thaumatin showed a potent inhibitory effect on stained lipid droplets at a concentration of 3 ${\mu}M$. Thaumatin reduced triglyceride accumulation in differentiated 3T3-L1 cells in a dose-dependent manner, compared with positive control cells. This study provides basic information on the anti-obesity activity of thaumatin.

• Korean Journal of Plant Resources
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• v.26 no.5
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• pp.619-627
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• 2013

Deutzia paniculata Nakai is a Korean endemic species that has a very restricted distribution in Gyeongsang-do, South Korea. The genetic diversity and structure of five populations of D. paniculata were investigated using 31 ISSR loci from six primers. The Shannon's index (0.429) and genetic diversity (0.271) were relatively higher than those of other rare plant species in Korea. The Miryang (MY) and Yangsan (YS) populations, which have higher flowering rates than the other populations, showed greater genetic diversity than the other populations. An analysis of the molecular variance (AMOVA) showed that 16% of the total variation could be attributed to differences among the populations, and 84% to the differences within populations, indicating moderate gene flow among adjacent populations. The high genetic diversity and low genetic differentiation in the Deutzia paniculata populations, which have a restricted distribution, is considered to be affected by outcrossing of the mating system and abundant individuals in the populations. These results suggest that ex situ conservation strategies are needed to sustain the current genetic diversity of D. paniculata.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.390-399
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• 1997

This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

• Journal of the Mineralogical Society of Korea
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• v.22 no.4
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• pp.371-383
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• 2009

Crystallinity and textural relations, which are crucial in terms of the quality concept of high-purity limestone, have not been effectively applicable to the limestone evaluation as crude ore due to the difficulties in precise measurements. To overcome the above, as a new method of ore characterization, a measurement of shape factor and grain boundary frequency utilizing the image analysing system was adopted in this study. Some domestic limestones used for lime manufacturing were investigated by such a quality evaluation method, and its results are discussed and correlated each other samples. As the result, even though calcite contents of crude ore, i.e., limestone grade and its crystal size are similar, quality of manufactured lime is remarkably different depending on the degree of shape factor and grain boundary frequency. In other words, as the more irregular in crystal shape and the higher the grain boundary frequency, the manufactured quick lime became more superior in all terms of lime quality such as rate of calcination, porosity, reactivity, sintering and decrepitation effect. However, because the quick lime become easily overheated in case of relatively higher degree in shape factor and grain boundary effect, a technology minimizing heating time is necessary for the manufacturing of high quality lime. In limestone industry, such a ore characterization method will be much more reasonable than the conventional method by measurement of mean size, because the method may collectively comprise crystal shape and other textural factors which can not be numerically evaluated in the past.

• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.