• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• The Korean Journal of Zoology
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• v.38 no.2
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• pp.248-268
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• 1995

출생 후 0일, 7일, 14일, 21일 그리고 성체의 흰쥐 전뇌 기저부의 내측중격핵과 수직 및 수평대각 Broca대에서 choline acetyltransferase(ChAT)에 면역반응을 보이는 신경조직과 세포의 분화를 면역조직화학적 및 전자현미경적 방법을 이용하여 조사하였다. 출생 후 초기와 성체에서 신경세포의 세포질과 수상돌기에서 고루 ChAT 면역반응이 확인되었다. 뇌 기저부의 ChAT 면역반응 신경세포들은 발생에 따른 뇌 크기의 증대와 뇌 조직의 분화에 따라 점차 수적 증가를 보였다. 이 ChAT 면역반응 신경세포들은 세포의 모양과 세포제의 장 · 단축의 비에 따라 6가지 형 즉 1) 월형 2) 난형, 3) 세장형, 4) 방추형. 5) 삼각형, 6) 다각형으로 분류되었다 전뇌 기저 핵에서 원형과 난형 신경세포들의 출현율은 출생 후 0일에서 높았으나 성체로 되면서 감소된 반면, 세장형. 방추형. 삼각형 그리고 다각형 신경세포들의 출현율은 출생 후 0일에서는 낮았으나 성체로 되면서 증가하였다. 모든 핵들에서 ChAT 면역반응 신경세포체의 부피는 출생 후 0일에 996-1,252 Um3로 제일 작았으며, 내측중격핵과 수직대각 Broca대 그리고 수평대각 Broca대에서는 출생 후 21일에 각각 5,061, 5.701, 5,820 um3로 최대치를 보였다. 그후 성체로 되면서 모든 핵에서1,897-2,704 roms로 다시 감소하였다. 전자현미경적 관찰에서 출생 후 21일된 흰쥐 수평대각 Broca대에서 ChAT 면역반응은 핵의 핵질 일부와 핵막 그리고 미토콘드리아와 조면소포체에서 관찰되었다. 이 결과들로 미루어 출생 후 초기 발생단계에서 흰쥐 전뇌 기저부의 내측중격핵과 수직 및 수평대각 Broca대에서 ChAT 면역반응 신경세포들은 축삭과 수상돌기의 형성에 따라 세포의 형과 그 출현율 및 세포제의 크기에서 현저한 변화가 이루어지는 것으로 생각된다.

• Journal of KIISE:Software and Applications
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• v.28 no.12
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• pp.875-884
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• 2001

Although the self organizing map (SOM) is widely utilized in such fields of data visualization and topology preserving mapping, since it should have the topology fixed before trained, it has some shortcomings that it is difficult to apply it to practical problems, and classification capability is quite low despite better clustering performance. To overcome these points this paper proposes the dynamic topology preserving self-organizing map(DTSOM) that dynamically splits the output nodes on the map and trains them, and attempts to improve the classification capability by combining multiple DTSOMs K-Winner method has been applied to combine DTSOMs which produces K outputs with winner node selection method. This produces even better performance than the conventional combining methods such as majority voting weighting, BKS Bayesian, Borda, Condorect and reliability sum. DTSOM remedies the shortcoming of determining the topology in advance, and the classification rate increases significantly by combing multiple maps trained with different features. Experimental results with handwritten digit recognition indicate that the proposed method works out to problems of conventional SOM effectively so to improve the classification rate to 98.1%.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1993.05a
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• pp.85-85
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• 1993

The clinical staging system for laryngeal cancers is not sufficient for prognosticator due to different biologic characteristics and their microenvironment according to primary sites. For determining the prognosticators, the authors peformed immunohistochemical staining to EGFR, p53 protein, and pRB in 40 cases of surgically treated squamous cell carcinomas of larynx in our institute during the past 5 years. The results are as followings; 1. The positive expression rate of p53 protein and negative expression rate of pRB showed correlations with clinical parameters. 2. The three-year survival rate for p53 protein positive cases was worse than the p53 protein negative cases. 3. Expression rate of EGFR was not correlated with the clinical parameters. As a conclusion, expression rates of p53 protein and pRB not only reflect well the biologic behavior of laryngeal cancer, but correlate closely with the tumor factors. Therefore they may be useful as the prognosticator to predict the malignant potency of laryngeal squamous cell carcinomas.

• Journal of Plant Biology
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• v.34 no.1
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• pp.19-23
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• 1991

Culture of embryogenic callus and suspension were induced from rice seeds in MS2.5 medium. In hormone free N6 medium, whole plantlets were regenerated from embryogenic callus. We observed cell division and reformation of embryogenic callus on culture of protoplast isolated from embryogenic cell suspensions. In addition, we studied the influencing factors on viability of protoplast treated with electroporation. Viability was decreased according to the increase of voltage and capacitance during electroporation. An optimal level of viability was obtained after treatment with $200-300\;V/1180\;\mu\textrm{F}$ in HEM buffer at $4^{\circ}C$..

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.414-421
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• 2016

Fourteen Acer pseudosieboldianum populations in South Korea were used to estimate genetic diversity, genetic differentiation and genetic relationships using seven AFLP primer combinations. The average of effective alleles ($A_e$), the proportion of polymorphic loci (%P) and Shannon's diversity index (I) was 1.4, 82.2% and 0.358, respectively. The expected heterozygosity ($H_e$) under Hardy-Weinberg equilibrium was 0.231 and the expected heterozygosity (Hj) from Bayesian inference was 0.253. The level of genetic diversity was moderate compared to those of Genus Acer and lower than those of other species having similar ecological niche and life history. The inbreeding coefficient within populations ($F_{IS}$) from Bayesian method was 0.712 and it could be influenced by selfing or biparental inbreeding to induce homozygote excess. The level of genetic differentiation was 0.107 from AMOVA (${\Phi}_{ST}$) and 0.110 from Bayesian method (${\Phi}^{II}$). The genetic differentiation was lower than those of other species having similar ecological niche and life history. Ulleungdo population had the lowest level of genetic diversity and was genetically the most distinct population from others in the study. We consider that founder effect and genetic drift might be occurred to reduce genetic diversity and then the geographical isolation might interrupt gene flow to aggravate it.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.47-52
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• 2003

Anthers from several lines of carrot (Daucus carota L.) were plated on the semi-solid B$_{5}$, basal medium supplemented with 2,4-D and NAA at two concentrations, 1.0 and 2.0 mg/L plus 0.2 mg/L BAP (benzylaminop-urine). Anthers of the most lines on the B$_{5}$ basal medium with 2,4-D showed higher percentages of callus formation than those with NAA. Particularly, in line 45477, highest percentages of callus formation (50%) were observed on B$_{5}$ medium with 1.0 mg/L 2,4-D plus 0.2 mg/L BAP. With 1.0 mg/L 2,4-D, two months was sufficient for initiation of callus development. Calli were regenerated into plantlets through embryogenesis onto regeneration medium without any growth regulators. When callus showing yellowish and soft structure was cultured, it yielded green plants at high regeneration rates, The response of anthers in callus induction and plant regeneration was different among lines investigated. Optimal callus induction and plant regeneration could be obtained through manipulating the concentration of growth regulators. Plantlets after transfer to perlite were grown successfully in greenhouse conditions. Anther culture of carrot will be used as a useful breeding tool in future.

• Tuberculosis and Respiratory Diseases
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• v.63 no.2
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• pp.165-172
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• 2007

Background: The pathogenesis of lung cancer includes the accumulation of multiple genetic abnormalities. The CREB-binding protein(CBP) is one of several transcriptional co-activators among various sequence-specific DNA-binding transcription factors. CBP is involved in a wide range of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis that are suspected of contributing to tumorigenesis. The goal of this study was to evaluate CBP expression in a series of human lung tissues containing normal epithelium, premalignant lesions(hyperplasia and dysplasia) and squamous cell carcinomas. Materials and Methods: Immunohistochemical staining was performed on formalin-fixed paraffin-embedded sections by use of a monoclonal anti-CBP antibody. CBP expression was compared in samples from 120 patients with premalignant and malignant histological types including 20 metaplastic specimens, 40 dysplastic specimens, and 60 squamous cell carcinomas in the lung. Results: CBP expression was seen in 35% (7/20) of the metaplastic specimens. 65% (26/40) of the dysplastic specimens, and 70% (42/60) of the squamous cell carcinomas (p<0.05). According to celluar atypism, CBP expression was 50% (10/20) of the low-grade dysplastic specimens and 80% (16/20) of the high-grade dysplastic specimens(p <0.01). By cellular differentiation, CBP expression was seen in 95% (19/20) of the well differentiated squamous cell carcinomas, 85% (17/20) of the moderately differentiated carcinomas and 30% (6/20) of the poorly differentiated lesions (p <0.05). Conclusion: These results suggest that CBP may have an important role in malignant transformation of precancerous lung lesions and may be a marker for malignancy.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.346-351
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• 2011

This research was done to identify the mass propagation method in winter daphne (Daphne odora Thunb) using its softwood cuttings and to investigate its plant characters established at different light and soil conditions as an indoor plant. Cuttings from winter daphne were taken and grown in different treatment consisted of rooting media (perlite, vermiculite, perlite + vermiculite (1 : 1) and commercial horticulture media soil), indole butyric acid (IBA) hormone concentrations (0, 100, 500, 1000 ppm and Rootone) and date of cutting. Transplants were grown at different light intensities (100, 1000, 2500 lux and control) and growing media. Results showed that cuttings grown in perlite + vermiculite (1 : 1) gave higher percentage (100%) rooting. Cuttings treated with Rootone and IBA 100 ppm showed good rooting growth and cutting taken in June, 25 gave the highest rooting (96.7%). The best plant growth obtained at 1000 lux (56~60 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) followed by 2500 lux (125~130 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and scoria mixed with commercial horticulture media soil showed better growth of transplants.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.86-86
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• 2019

현삼과(Scrophulariaceae)에 속하는 긴산꼬리풀(Veronica longifolia L.)은 벽자색의 긴 꽃이 총상화서로 달려 청량감을 자아내어 분화소재로 이용한다면 단조로운 실내의 분위기를 환기할 수 있다. 따라서, 조경 및 관상용 식재에 용이한 긴산꼬리풀의 재배는 2018년 5월 15일에 파종하여 생산된 유묘를 2018년 7월 16일에 정식하여 2018년 10월 15일까지 수행하였다. 기비실험은 코트비료(싱싱코트)를 포트당 0, 10 및 20알 처리하였다. 추비는 hyponex (N-P-K, 4-6-6)를 4주 간격으로 총 3회 각 0, 1000 및 $2000mg{\cdot}L-1$로 엽면시비 하였다. 광량 실험은 무차광, 55 및 75% 차광막을 설치하여 수행하였다. 왜화제 종류 및 농도 실험은 dinizonazole(빈나리)과 daminozide (B-9)를 각 0, 1000 및 $2000mg{\cdot}L-1$ 농도로 처리하였으며, 적심처리는 4주차에 1회 처리 유무를 달리하였다. 모든 실험의 공통사항은 플라스틱 10호 화분에 원예상토를 충진한 후 200구 트레이에 종자를 셀당 4립씩 파종하여 생산된 유묘를 정식하였다. 연구의 결과, 기비처리는 초장, 마디수, 엽수가 증가하고, 절간장 및 측지수를 감소시켰으나, 유의적인 차이는 나타나지 않았다. 추비 처리는 농도가 높을수록 엽수가 증가하는 경향을 보였으며, $2000mg{\cdot}L-1$에서 무처리에 비해 유의적으로 많았다. 차광정도에 따른 긴산꼬리풀의 생육은 무차광 조건에서 생육이 가장 양호하였다. 차광률이 증가할수록 전반적인 생육이 억제되고, 개화율도 크게 감소하는 결과를 보였다. 왜화제 처리별로는 종류에 관계없이 전반적인 생육이 억제 되었다. Diniconazole 및 daminozide의 농도가 높을수록 초장이 억제되는 경향을 보였으나, 측지수, 마디수, 엽수도 감소하여 관상적 이용에 적합하지 않다. 적심을 할 경우 전반적인 생육은 큰 차이가 나타나지 않았으며, 측지수가 감소하였다. 따라서, 긴산꼬리풀의 분화생산을 위한 재배기술은 기비는 처리하지 않고, 추비는 $2000mg{\cdot}L-1$로 시비하며, 광이 충분한 곳에서 재배하는 것이 효과적이다. 또한, 왜화제와 적심은 일반적으로 줄기신장을 억제하고 마디수와 엽수를 증가시켜 관상적 이용효과가 있는 것으로 알려져 있지만 본 연구에서는 반대의 결과를 보여 처리 하지 않는 것이 효과적인 것으로 판단된다.