• Journal of Digital Convergence
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• v.16 no.4
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• pp.39-47
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• 2018

This paper aim to propose and suggest for government to reinvent and re-steering the policy on the 4th industrial revolution in Korea. To do it, this paper annalize the advanced country's policies and hard-data sets from many international research institutes. So this paper emphasized the concept and frame of socio-technical system approach by Geels. According to this approach, this paper can get a conclusion that Korea government has to focus on the roles to solve the big social dilemma with high-tech tools from the 4th industrial revolution. And this paper point out the necessary of increasing the diversity and multidisciplinary in 4th industrial revolution policy of Korean government. These findings of this paper recommend for Korea to redesigns the governance mechanism and legislation platform and to design the future readiness.

• Journal of the Korean Association of Geographic Information Studies
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• v.26 no.1
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• pp.114-131
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• 2023

The study empirically delves into the longitudinal transition of the urban core of Naju City in Korea. For the purpose, the ASA (Angular Segment Analysis) technique of Space Syntax has been adopted to investigate the cadastral maps on the GIS platform for the five chosen years since 1920. In particular, the global integration map as well as box plot statistics have been used to capture the time-series consequences. The findings indicate that the old downtown is no more a monocentric city core and the center of the City has far moved eastward near the new Gwangju-Jeonam Innovation City especially during the period between 2000 and 2020.

• Economic and Environmental Geology
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• v.47 no.2
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• pp.133-146
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• 2014

There are many studies for the economic impact assessment of natural disasters, but there are few for volcanic disasters. Domestic academic research is not under active discussion because of the lack of national and social interest for volcanic eruption. This study investigated the research methodology for the economic impact assessment of natural disasters and discussed whether these can be applied to the economic impact analysis for the Baekdu Mountain volcanic disaster. The main findings are as follows: Firstly, Asia-Pacific region is the most affected by natural disasters and has the largest scale of damage. Asian and American Continent have the most economic damage. Secondly, Considering the types of damage caused by natural disasters and its complex structure, several methodologies that could be possible to estimate economic consequential damages have been compared. When applying each methodology to the Baekdu Mountain volcanic disaster, the scale of damage is likely to be over-estimated or under-estimated because of model-specific features. Thus, estimated values should be compared to each other after calculating the damage results. Thirdly, Japanese academic research on the volcanic disaster will be used as the starting point of the economic impact assessment studies for Baekdu Mountain. Using computer SW such as Hazus which is used in United States and RiskScape from New Zealand is also a good method to predict economic impact of the Baekdu Mountain volcanic disaster.

• Clinical and Experimental Reproductive Medicine
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• v.36 no.4
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• pp.311-319
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• 2009

Objective: Human embryonic stem cells (hESCs) have the capacity to differentiate into all of the cell types and therefore hold promise for cell therapeutic applications. In order to utilize this important potential of hESCs, enhancement of currently used technologies for handling and manipulating the cells is required. The cryopreservation of hESC colonies was successfully performed using the vitrification and slow freezing-rapid thawing method. However, most of the hESC colonies were showed extremely spontaneous differentiation after freezing and thawing. In this study, we were performed to rapidly collect of early passage hESCs, which was thawed and had high rate of spontaneously differentiation of SNUhES11 cell line. Methods: Four days after plating, partially spontaneously differentiated parts of hESC colony were cut off using finely drawn-out dissecting pipette, which is mechanical separation method. Results: After separating of spontaneously differentiated cells, we observed that removed parts were recovered by undifferentiated cells. Furthermore, mechanical separation method was more efficient for hESCs expansion after thawing when we repeated this method. The recovery rate after removing differentiated parts of hESC colonies were 55.0%, 74.5%, and 71.1% when we have applied this method to three passages. Conclusion: Mechanical separation method is highly effective for rapidly collecting and large volumes of undifferentiated cells after thawing of cryopreserved early passage hESCs.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.3
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• pp.171-178
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• 2006

Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

• Journal of Life Science
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• v.31 no.4
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• pp.385-388
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• 2021

Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.

• Clinical and Experimental Reproductive Medicine
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• v.37 no.1
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• pp.13-23
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• 2010

Objective: The present study was carried out to induce differentiation of human embryonic stem cells (hESCs) into germ cells and to establish a culture condition for single hESCs dissociated by enzyme. Methods: Embryonic body (EB) was formed by hanging drop culture for 3 days from hESCs colony. The EBs were cultured in the medium supplemented with retionic acid (RA) or/and bone morphogenetic protein-4 (BMP4) for 14 days to differentiate into germ cells. Germ cell specific markers, c-kit and VASA were used for immunohistochemistry of EB. Human ESCs colonies were dissociated into single cells by Collagenase, Tryple and Accutase, and then colony formation rate of the single cells was examined. Rho-associated kinase inhibitor (ROCK inhibitor, Y27632) was added into the culture medium of single cells to reduce the apoptotic damage during the dissociation. Results: Single cells dissociated with Tryple or Accutase showed higher colony formation rates compared to the cells dissociated with Collagenase. Seeding of $5{\times}10^3$ cells/well (4 well dish) was efficient to obtain high colony formation rate compared to other concentrations of seeding cell. Addition of Y27632 significantly increased the colony formation rate of the single cells dissociated by Tryple. Immunohistochemistry of EB with c-kit and VASA markers showed a weak fluorescence signals compared to the signals from the testicular tissue. Conclusion: Dissociation with Tryple was useful to obtain healthy single cells and addition of Y27632 was beneficial for survival and colony formation of the single cells. Unlike other studies, we just observed a dim fluorescence staining of the germ cell markers, probably caused by the short-term culture for the differentiation of EB compared to other studies.

• Journal of Korean Society for Geospatial Information Science
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• v.20 no.3
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• pp.3-10
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• 2012

The investigation of valley networks and their volume provide important information about past water activities on Mars. As an alternative of conventional image processing methods, terrain filtering algorithm using pointcloud data is suggested in this study. First, the topography of pointcloud is inverted so that the valleys become positive features and the algorithm is then applied to distinguish the valleys from the surface. Ground DEM and object DEM are generated from both the valleys and the surface pointcloud then the volume of valleys is estimated by multiplying the height difference between the surface with valleys and the area of valleys based on grid cellsize. In the test of valleys adjacent to Tuscaloosa crater, the total volume of valleys was estimated to be $1.41{\times}10^{11}m^3$ with the difference of 12% and 16% compared with the infill volume of Tuscaloosa crater and BTH result respectively.

• Journal of the Korean Geographical Society
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• v.31 no.2
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• pp.268-294
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• 1996

Social and population geography in Korea has been developed somewhat later than other special subjects of geography. But in recent years, it has attracted much interests of geographers, and accumulated a considerably large amount of research products. Seen in this context, it is resonable to expect that social and population geography will not only lead the development of geography in the future, but also contribute to the resolution of socio-spatial problems in Korea. From this point of view, we shall retrospect in this paper the progress of social and population geography in Korea since the 1950s, and look out its future prospect and further research tasks. In the first place, we discuss general concepts and major themes of social and population geography, and overview its development process, and than review in more details some important research products which would be categorized into five main subjects, that is, research methodology, urbanization and urban population phenomena, population movement and changing rural life, residential differentiation and urban redevelopment, and urban social life and regional social well-being. Finally, we consider briefly the future prospect and further tasks of social and population geography in Korea.

• Journal of KIISE:Software and Applications
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• v.31 no.12
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• pp.1569-1580
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• 2004

One commonly used approach to deal with uncertainty is Bayesian network which represents joint probability distributions of domain. There are some attempts to team the structure of Bayesian networks automatically and recently many researchers design structures of Bayesian network using evolutionary algorithm. However, most of them use the only one fittest solution in the last generation. Because it is difficult to combine all the important factors into a single evaluation function, the best solution is often biased and less adaptive. In this paper, we present a method of generating diverse Bayesian network structures through fitness sharing and combining them by Bayesian method for adaptive inference. In order to evaluate performance, we conduct experiments on learning Bayesian networks with artificially generated data from ASIA and ALARM networks. According to the experiments with diverse conditions, the proposed method provides with better robustness and adaptation for handling uncertainty.