• Title/Summary/Keyword: 분리력

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Isolation and Characterization of Rhizobia from Soybean Cultivated in Korea (대두(大豆) 근류균(根瘤菌)의 분리(分離) 및 특성(特性))

  • Yun, Han-Dae;Cho, Moo-Je;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.30 no.2
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    • pp.153-162
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    • 1987
  • Soybean rhizobia were isolated from 101 soybean (Glycine max.) cultivar which had been grown for the breeding experiment in Korea. Seven strains of the fast-growing soybean rhizobia and nine strains of the slow-growing soybean rhizobia were selected on the basis of their growth rate in AMA medium and their high ability of nodulation. The slow-growing soybean rhizobia were identified as Bradyrhizobium japonicum in the acetylene-reducing activity, microbial characteristics, and biochemical characteristics whereas the fast-growing soybean rhizobia were very similar to Rhizobium fredii.

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Isolation and Identification of Red Color Pigments from the Korean Lithospermum erythrorhizon (한국산 자초 적색소의 분리 및 확인)

  • 이제헌;오문헌;이희봉
    • The Korean Journal of Food And Nutrition
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    • v.13 no.4
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    • pp.379-382
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    • 2000
  • Conditions of isolation for the red color pigments from the Korean Lithospermum erythrorhizon were investigated and identification of the red pigment was analysed. Non-polar solvents were more effective than water. Especially, 95% ethanol was observed as optimum solvent for the pigments extraction. About 20 minutes at 40$^{\circ}C$ with 95% ethanol was enough for the extraction of the red pigments. The major pigment was analysed as acetylshikonin by TLC, IR, NMR and GC/MS.

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Isolation of Antioxidant and Antibrowning Substance from Chionanthus retusa Leaves (이팝나무 잎으로부터 항산화 및 항갈변물질의 분리)

  • Lee, Young-Nam;Jeong, Chang-Ho;Shim, Ki-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.9
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    • pp.1419-1425
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    • 2004
  • This study was performed to examine in vitro antioxidative activities such as DPPH radical scavenging activity, reducing power and tyrosinase inhibitory effect of various solvent fractions from Chionanthus retusa leaves. Ethyl acetate fraction showed potent antioxidative activity and tyrosinase inhibitory effect. The active compound was isolated from the butanol fraction by silica gel column chromatography and MPLC. The isolated compound was luteolin-4'-O-glucoside determined by $^1H$, $^{13}C$-NMR and 2D NMR. Compared with several antioxidant compounds, luteolin-4'-O-glucoside exhibited effective DPPH radical scavenging activity and reducing power in a concentration dependent manner. Bioassay with pure luteolin-4'-O-glucoside showed a dose-independent inhibitory effect on L-DOPA oxidation by mushroom tyrosinase and its $IC_{50}$ values were established as 23.2 ${\mu}g/mL$. Therefore, we may suggest that luteolin-4'-O-glucoside can be used as a food additive possessing the potent antioxidative activity and skin-whitening cosmetic material.

Isolation and Application of Phytate-Hydrolysing Lactic Acid Bacteria. (Phytate 분해 유산균의 분리 및 이용)

  • 양시용;송민동;김창원;유제현;정기철
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.195-200
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    • 2001
  • Among lactic acid bacteria isolated from young animal feces, a bacterium having high phytate degradation ability, identified as L. paracasei subsp. paracasei, was selected. When inoculated into soybean meal, wheat bran and rice bran, the bacterium showed phosphate group of phytate (phytate-P) degrad- ability of 27.07% for soybean meal and 12.18% for wheat bran. However, degradation of phytate-P was not observed for rice bran L. paracasei subsp, paracasei had good acid and bile juice tolerance, having 9.70, 9.66 and 8.80 (log CFU/ml) for control, acid and bile juice treatment. Feed efficiency increased from 3.71 to 3.21 with addi- tion of the bacterium at 0.4% (w/w) level in swine fattener\`s diets.

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A Study on the Mechanism of Recycled Sand Dry Manufacturing System (순환잔골재 건식생산시스템의 메커니즘에 관한 연구)

  • Choi, Hyeong-Gil;Kim, Young-Bong;Na, Chul-Sung;Lee, Eui-Bae;Kim, Gyu-Yong;Kim, Moo-Han
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.481-484
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    • 2008
  • Recently, the recycling and reusing of construction and demolition waste concrete is urgently required because waste concrete is greatly increased according to the rapid increasing of urban redevelopment project, but the problem solution for demand and supply unbalance of fine aggregate is urgently required because of the restriction of collecting sea fine aggregate. So the utilization of high quality recycled fine aggregate using construction and demolition waste concrete as new fine aggregate for construction industry is urgently. Accordingly, In this study, As recycled fine aggregate manufacturing technology with exceeding in economical efficiency, reduction efficiency of environmental load and quality improvement effect of recycled fine aggregate, it is to develop dry manufacturing system composed specific gravity separator of high-speed rotation impact type and centrifugal Force Powder Collector, etc. And it is to examine mechanism of recycled sand dry manufacturing system.

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Removal of Cesium using Carbonyl iron-Prussian blue complex material in water (카보닐 철-프러시안 블루 합성체를 통한 수중의 세슘 제거)

  • Kim, Jong Kyu
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.198-198
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    • 2019
  • 2011년 동일본 지역에서 발생한 지진으로 인하여 후쿠시마 다이이치에 위치한 원자력 발전소에서 다양한 방사성 물질들이 바다, 하천 그리고 대기와 같은 자연환경 속으로 유출되었다. 방사성 세슘(Cesuim, $Cs^{137}$)은 다양한 방사성 물질들 가운데 반감기(Half-life)가 30.17년으로 가장 긴 물질이다. 방사성 세슘이 환경 생태계로 한번 유출될 경우 긴 반감기과 널리 퍼지는 성질로 인하여 오랜 시간동안 넓은 지역에 막심한 피해를 초래하므로 효과적인 처리방법을 통해 안전하게 처리하는 것이 아주 중요하다. 세슘을 제거하기 위하여 물리적, 화학적, 생물학적 등 다양한 방법들을 통해 연구를 진행하고 있으며, 특히 세슘을 제거하는 아주 효과적인 방법 중 하나인 프러시안 블루(Prussian Blue, PB) 흡착제를 적용하는 방법이 많이 주목받고 있다. 그러나 프러시안 블루는 미세한 분말입자로서 수처리에 사용하게 되면 처리 후 발생되는 슬러지들을 수중으로 부터 분리하기 어려운 한계점을 가지고 있다. 최근 연구에서는 프러시안 블루의 적용 한계점를 극복하기 위하여 자성체(Magnetic substance)를 물리적 지지체로 이용하여 외부 자기장을 통해 수중으로 분리하는 방법들이 연구되고 있다. 자성체란 외부 자기장이 주어지게 되면 입자들 표면에 자성력을 띄는 물질들을 말한다. 본 연구에서는 자성체 종류들 가운데 가장 높은 자성력을 지닌 강자성체(Ferromagnetic Substance)를 물리적 지지체로 하여 산화과정, 실란과정, 합성과정을 거쳐 강자성체 입자의 표면에 프러시안 블루를 합성한 새로운 형태에 합성체를 제조하고, 제조된 합성체를 이용하여 수중에 존재하는 세슘 제거 능력을 평가하였다. 제조된 합성체의 물리적 특성을 분석하기 위하여 SEM, XRD를 이용하여 합성체 입자의 표면 분석을 진행하였다. 합성체의 세슘 제거 능력을 평가하기 위하여 임의 제조된 0.5mg/L의 세슘 농도를 가진 원수 100ml에 제조된 새로운 형태의 합성체 1g을 투입한 뒤 1분간의 반응시간 동안 반응한 이후 잔류 세슘을 측정한 결과 수중의 존재하는 세슘에 대해 99.9%의 세슘 제거율을 기록하였다. 자가분리(Magnetic Seperate)의 원리를 이용하여 수중으로부터 회수율을 측정한 결과, 99%의 합성체 회수율을 얻었다. 실험결과를 통해 외부자기장이 주어지게 되면 수중으로부터 합성체를 대부분 분리하여 회수할 수 있다고 판단된다. 본 연구를 통해 개발된 새로운 형태의 합성체는 수중의 세슘 처리 공정에서 사용자가 직접 접촉하지 않고 세슘제거 및 외부자기장을 통해 수중으로부터 분리가 가능한 합성체라고 판단된다.

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Physiological Characteristics of Bacillus spp. Isolated from Rice Straw as Cheonggukjang Starter (볏짚에서 분리한 청국장 starter용 Bacillus spp.의 생리적 특성)

  • Lee, Shin-Ho;Baek, Lag-Min;Park, La-Young
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.562-567
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    • 2008
  • The primary objective of this study was to select a probiotic starter for cheonggukjang using 60 strains isolated from rice straw. Among isolated strains, only 8 strains including strain B-59 evidenced proteolytic, amylolytic and soybean activity. These 8 strains were all gram-positive, spore-forming rods. The B-59 strain evidenced antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Pseudomonas fluorescens, and Vibrio parahaemolyticus. The antimicrobial activity of isolated B-59 was verified by its ability to inhibit the growth of S. aureus, L. monocytogenes, P. fluorescens, and V. parahaemolyticus. The selected B-59 strain was indentified as Bacillus licheniformis, as shown by a result of 99.0% homology upon API kit analysis. The selected B-59 strain also displayed viability in pH 2.5 artificial gastric juice, artificial bile acid, NaCl (2, 4, 8, 16, 32%), and ethanol (4, 8, 16, 32%). The antioxidative activity of the strain B-59 culture was assessed via a DPPH free radical scavenging activity assay. The activity increased with an increasing in the fermentation time of strain B-59 for 20 hours.

A Study on Selection of Split Variable in Constructing Classification Tree (의사결정나무에서 분리 변수 선택에 관한 연구)

  • 정성석;김순영;임한필
    • The Korean Journal of Applied Statistics
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    • v.17 no.2
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    • pp.347-357
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    • 2004
  • It is very important to select a split variable in constructing the classification tree. The efficiency of a classification tree algorithm can be evaluated by the variable selection bias and the variable selection power. The C4.5 has largely biased variable selection due to the influence of many distinct values in variable selection and the QUEST has low variable selection power when a continuous predictor variable doesn't deviate from normal distribution. In this thesis, we propose the SRT algorithm which overcomes the drawback of the C4.5 and the QUEST. Simulations were performed to compare the SRT with the C4.5 and the QUEST. As a result, the SRT is characterized with low biased variable selection and robust variable selection power.

Missile Flight Condition for Slip-in Booster's Safe Separation (내삽형 부스터 안전 분리를 위한 비행 조건 연구)

  • Oh, Hyun-Shik;Lee, Ho-Il;Cho, Jin;Kim, Ik-Soo
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.39 no.1
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    • pp.33-41
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    • 2011
  • A mathematical model of slip-in booster separation dynamics is described. A longitudinal 3-DOF(degree of freedom) 2-body dynamic model is developed to simulate the separation dynamics. Aerodynamic models of the missile and the exposed area of booster are built. And, gas generator pushing the booster out and internal channel pressure drop are modelled. To simulate the model, it is assumed that the missile can maintain the 1g level-fight condition during the separation. With this assumption, the interaction forces between missile and booster through the separation phases: phase 0: initial, phase 1: linear translation, and phase 2: free flight motion are defined. Using the simulation, missile flight conditions for slip-in booster`s safe separation, which can be represented by Mach vs. height envelope, are suggested.

A Study on the Power Transaction of Operating Reserve with Consideration of Marginal Profit (발전예비력을 포함한 전력시장에서 한계이득을 고려한 전력거래연구)

  • Shin, Jae-Hong;Lee, Kwang-Ho
    • Proceedings of the KIEE Conference
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    • 2006.07a
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    • pp.374-375
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    • 2006
  • 전력시장이 경쟁체제로 바뀜에 따라 전력거래소는 적정예비력을 에너지 시장과 분리된 예비력 시장을 통해 확보해야 한다. 발전사업자는 두 시장에서 이득을 얻을 수 있으므로 이득을 고려하여 전략적인 입찰함수를 결정한다. 본 연구에서는 에너지 시장과 예비력 시장을 모형화하고 lemke 알고리즘을 사용하여 경쟁으로 나타나는 균형점을 해석한다. 또한 균형상태의 시장실적을 분석하여 발전사업자의 전략적인 행태를 분석한다. 마지막으로 예비력 시장과 에너지 시장의 참여유인을 한계이득과 관련지어 분석한다.

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