• Applied Chemistry for Engineering
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• v.4 no.3
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• pp.583-590
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• 1993

O-Diethylaminoethyl chitosan containing weak cation exchange group had been synthesized. It was used to separate albumin and lysozyme as stationary phase in liquid chromatography column. The separation of acidic and basic dyes were preliminarily conducted to investigate the effect of polarity parameter of solvents. Based on this result, we investigated various factors on the separation efficiency of chromatography in column. In the case of using water-methanol as cosolvent, the resolution factor depended on the polarity parameter. The best resolution factor was 1.42 for DEAE-chitosan with the degree of substitution of 0.23.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.234-234
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• 1994

최근 새롭게 조명된 생약에 관한 여러 연구를 통해, 기존의 항암제 보다는 더 효과적이고 인체에는 부작용이 적은 항암제를 개발해내기 위해 국내 자생 생약중 총 103 종류( 95 손 98종 )을 채집하였다. 이들 생약을 암세포주( L1210, P333 D$_1$)와 장상세포주(Vero)을 대상으로 MTT colorimetric assay를 실시하여 항종양성에 대해 알아보았다. 이들 세포주에 대한 $IC_{50}$/ 값으로 세포독성능을 알아보았다. 그리하여 정상세포주에는 적은 세포 독성능을 나타내면서, 암세포주에는 높은 세포 독성능을 나타내는 생약제 6종( BuOH 추출물 2종, MeOH) 추출물 4종 )을 선정하였다. 이중 항암활성능이 가장 높은 미역줄나무(Tripteryrium regelii)를 선택하여 유기용매별로 추출, 그 각각에 대한 세포 독성능이 가장 높게 나타난 2분획을 선택하여 기존에 시판중인 Adriamycin과의 병용 투여시의 세포 독성능의 상승효과를 확인 하였다. 즉 Adriamycin과의 단독 투여보다 복합 투여시에 암세포주에 대한 세포 독성능이 높아졌고, 정상세포주에 대한 독성이 감소되는 효과가 나타났다. 또한 in vitro 에서는 세포 독성능이 다소 적더라도 in vivo에서 면역학적 활성이 기대되는 생약제 3종을 선정하여 항암성분의 분리 및 정제를 하여 항암성에 대하여 알아보았다.

• Korean Journal of Food Science and Technology
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• v.38 no.4
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• pp.534-542
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• 2006

The protein contents of soy protein isolate (SPI) and soft wheat flours were 83.5% and 8.5%, respectively. The addition of SPI increased the protein content but decreased the sedimentation value. Alkaline water retention capacity (AWRC) value increased with SPI addition and was highly correlated with protein content. Increasing SPI flour content significantly decreased the maximum, minimum and final viscosities. Mixograph peak time was positively correlated with protein content and AWRC. The PH and specific gravity of the cake batter increased with increasing SPI content. The SPI addition reduced the loaf and specific loaf volume compared with soft wheat flour. The lightness of the cake crust decreased, while the redness and yellowness increased, with SPI flour addition. SPI addition resulted in a decrease of overall acceptability, but an increase in hardness.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.923-926
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• 2008

Currently more high flow and high performance concrete is used for construction of buildings in the world. However, when high flow and high performance concrete put high performance water reducing agent in quantity to improve flow, it has a negative effect on concrete structures since segregation arises from it though flow will be improved. There are naked-eye observation, coarse aggregate washing test, L Flow test for permeation among reinforcing rods and measurement of viscosity to judge concrete segregation resistance. However, it is difficult to apply them to practical affairs since they are very complicated and troublesome. Therefore, the study analyzed EIS dividing slump flow value into slump value, how to valuate concrete segregation resistance more easily, on the basis of the existing reference materials to propose EIS. As the results, in the event of high flow concrete, it is desirable that EIS value is prescribed to be less than 2.5 at the time of managing segregation. Also, at the time of prescribing EIS with performance, it is judged that it is desirable to manage segregation as less than 2.2 (Grade 1), 2.2$\sim$2.4 (Grade 2) and more than 2.6 (Grade 3).

• Analytical Science and Technology
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• v.21 no.6
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• pp.495-501
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• 2008

Agilent 5973 GC-MS instrument was modified so that the capillary direct interface was removed and that a silicone membrane was installed between GC and MS. Feasibility study of the membrane interface GC-MS has been carried out. Vacuum of the mass spectrometer was not affected by the carrier gas flow rate up to $4.7m{\ell}/min$. As the carrier flow rate was increased, peak tailing was reduced and chromatogram peaks appeared earlier. Chromatogram peaks showed better separation and higher sensitivity as the membrane thickness was reduced from $127{\mu}m$ to $75{\mu}m$, and also as the interface temperature was increased. However, the membrane interface GC-MS had drawbacks such as background ions at 73 and 147 m/z and poor peak separation due to peak tailing.

• Journal of Life Science
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• v.21 no.10
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• pp.1428-1433
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• 2011

In this study, we isolated and characterized plant-associated lactic acid bacteria which are able to produce angiotensin-converting enzyme (ACE) inhibitory and antioxidative activities. Five lactic acid bacteria were isolated from plants (grape and leek), a plant-associated fermentative product (Kimchi) and Korean traditional alcohol (Dongdongju). Strains K-1 and K-21 from Kimchi, strain L-5 from leek, strain G-3 from grape, and strain D-3 from Dongdongju were identified as Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum, and L. brevis, respectively, by 16S rRNA gene analysis. ACE inhibitory activities of isolated strains ranged from 44.3 to 71.9% in the MRS broth. G-3, L-5 and K-1 strains especially showed high ACE inhibitory activities (59.8-98.69%) in the MRS broth containing skim milk. DPPH radical scavenging activities of the strains were in the range of 42.5-82.7%. All strains showed varying levels of resistance in artificial gastric fluid (pH 2.5), retaining viability ranging from 42.2 to 88.1% after 3 hr of incubation. All strains showed high resistance to 0.3% oxgall after 24 hr of incubation; survival rates were in the range of 55.4-112.8%. Isolated strains were found to be antagonistic to some pathogens including Pseudomonas aeruginosa.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.4
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• pp.141-150
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• 2006

To develop the microbial agents for the environmentally-friendly treatment and recycling of food waste, useful microorganisms, which showed higher degradation activities to various organic compounds and possessed flocculating activities, were isolated from the earthworm. One of the isolated strains, named JS-01, was further selected due to its higher flocculating activity against 0.5% Kaolin clay. JS-01 was identified as Bacillus pumilus sp. by 16s rDNA analysis. The optimal temperature and pH for the growth of JS-01 to express the flocculating activity was found to be at $37^{\circ}C$ in pH 7.0 of EPS broth medium. JS-01 also expressed good degrading activity against cellulose, which is one of the representative organic materials in food waste. We propose that JS-01 will be a good candidate for the efficient treatment of food waste and leachate due to the property to degrade cellulose and flocculating activity.

• Membrane Journal
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• v.6 no.2
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• pp.59-71
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• 1996

기체 막분리공정 기술이 점점 개발되어질수록 막분리의 성능을 이해하려는 필요성이 각 공정에서 증진될 것이며 기체 막분리 성능의 예측능 기술 발전을 위해 계속 시도되어질 것이다. 이러한 추세에 힘입어 현재 석유화학공정 배가스 중 수소를 정제하기 위한 기술 개발을 시도하고 있으며 특히 저농도의 수소를 고농도로 농축시키기 위해 막분리 공정을 적극 검토하고 있다. 본 논문에서 밝혀 본 막분리 공정의 성능 예측과 분석은 향후 공정을 설계하고 제작하는 데 크게 이바지할 뿐만 아니라 석유화학 제반 공정뿐만 아니라 관련 화학공업장치 산업에서 기체 분리를 통한 자원회수와 에너지 절약 측면에서 계속 발전해 나갈 것은 믿어 의심치 않을 것이다.

• Ceramist
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• v.7 no.6
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• pp.76-81
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• 2004

가동온도가 $800^{\circ}C$ 이하인 평판형 SOFC에서는 기존의 세라믹 분리판 대신에 금속 분리판이 적용될 가능성이 높아지고 있다. 금속 분리판은 가공성 및 경제성 등이 세라믹 분리판 보다 우수하여 SOFC의 실용화를 앞당기는데 크게 기여할 수 있을 것으로 기대되고 있다. 그러나 금속 분리판의 적용을 위하여서는 아직 해결되어야만 할 문제점들이 남아 있는데, 표면 산화층의 형성에 따른 접촉 저항의 증가와 합금 성분 중의 Cr의 증발에 의한 전극 열화의 문제 등이 대표적인 그것이다. 이러한 문제점을 극복하기 위하여 SOFC용 분리판의 요구특성에 적합한 신합금의 개발과 고기능 표면 코팅 기술에 대한 연구가 활발하게 진행되고 있다.

• Korean Journal of Food Science and Technology
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• v.47 no.1
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• pp.44-50
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• 2015

The purpose of this study was to characterize the lactic acid bacteria found in makgeolli in terms of bacterial identity and gastric compatibility. Lactic acid bacteria were isolated from commercial raw makgeolli and separated into six strains that are resistant to gastric acidity and bile acid. These strains were identified by analysis of their 16S rDNA, as Lactobacillus plantarum BSM-2 and EHJ-1, Lactobacillus casei GSM-3 and EHJ-2, Lactobacillus brevis BSM-3 and Pediococcus pentosaceus TJH-1. All strains exhibited adhesion to intestines, showing that they were probiotic. We also found that L. plantarum BSM-2 had excellent resistance to bile acid as well as antioxidant activity. Taken together with its antibacterial properties and ability to lower cholesterol, our data suggest that L. plantarum BSM-2 was the most beneficial probiotic among the six strains.