• Title/Summary/Keyword: 분리공시

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Identification of Antifungal Activity Subatnces on Seedborn Disease from Garlic and Taxus Extracts (마늘, 주목의 추출물로부터 종자전염성병에 대한 항균활성물질 동정)

  • Chung, III Min;Paik, Su Bong
    • Analytical Science and Technology
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    • v.12 no.1
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    • pp.47-52
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    • 1999
  • Antifungal substances were isolated and identified from garlic and taxus extracts to develop safe and broad fungicide. The inhibitory effect of seedborn disease of sesame, pepper, radish, chinese cabbage by formulation of antifungal substances was investigated. The antifungal substance isolated through column chromatography from garlic and taxus extracts was confirmed by GC-MS as allicin($C_6H_{10}OS_2$) and taxol($C_{47}H_{51}NO_{14}$) and the quantified content from each extracts by HPLC analysis was 0.62%, 0.29%, respectively. The formulation composed of garlic and taxus extracts controlled effectively the seedborn fungi tested in this study at 10X dilution, but at 100X dilution the inhibitory effect decreased. Phytotoxicity of these formulations did not recognized.

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해외기업인수(海外企業引受)의 전략적(戰略的) 의의(意義) 및 주가(株價)에 미치는 영향(影響)

  • Jeong, Hyeong-Chan
    • The Korean Journal of Financial Management
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    • v.9 no.2
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    • pp.271-293
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    • 1992
  • 연구에서는 해외기업 인수의 생존전략가설(生存戰略假說)(Shapiro,1989)을 $1986{\sim}1991$년까지 해외기업을 인수한 30개 국내 인수기업의 주식수익률 자료를 이용하여 검증하였다. 해외기업 인수에 관한 정보의 공시가, 전체표본(全體標本)의 경우, 국내 인수기업의 주식가격에 중립적이거나 혹은 부정적인 효과를 가져다 주었다. 또한, 국내 인수기업중에서 외국기업을 인수해야 할 긴박성의 정도에 따라 전체표본을 성장기업(成長企業)과 한계기업(限界企業)으로 분리하여 각 표본을 대상으로 인수에 관한 정보의 공시가 주가(株價)에 미치는 효과를 분석하였다. 소표본의 분석 결과에 따르면, 성장기업의 경우는 초과수익률이 통계적으로 0에 가까운 중립적인 반응을 보였으며, 한계기업의 경우는 통계적으로 유의한 마이너스 초과수익률을 나타내어 생존전략 가설을 확인시켜 주었다. 따라서, 국내기업에 의한 해외기업 인수는 국제화를 통해 초과수익을 추구하기 위한 전략이라기 보다는, 국내 및 국제시장에서 살아남기 위한 생존전략의 한 방안으로 추진된다고 결론지을 수 있다.

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Inheritance of Scentedness of Rice Leaf in a Scented Breeding Line 'P-33-C-19' (향도의 방향성 유전)

  • 박순직
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.1
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    • pp.11-14
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    • 1984
  • The inheritance of scent in rice leaf and its genetic association with base color, hull color and resistance to bacterial leaf blight were studied in F$_2$ population of two rice crosses, P-33-C-19 (scented)/Zhu-Lian-Ai and P-33-C-19/Kataktara DA2. The scent was identified by leaf analysis at tillering stage. The scented and non-scented plants segregated in the ratio of 1scented: 3non-scented showing that a recessive gene responsible for the expression of scentedness. The scentedness was independently segregated with base color. hull color and resistance to bacterial leaf blight.

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A new steel jacketing method for RC columns and a modified constitutive model of jacketed concrete (RC 기둥 보강을 위한 새로운 강판 보강기법 및 수정 연속체 모델)

  • Tae, Ghi Ho;Choi, Eun Soo
    • Journal of Korean Society of Steel Construction
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    • v.20 no.5
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    • pp.675-681
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    • 2008
  • This study introduced a new steel-jacketing method to retrofit RC columns. It also estimated the performance of steel-jacketed concrete cylinders. Twelve concrete cylinders were fabricated with varying steel jacket thicknesses of 1.0, 1.5, and 2.0 mm. Lateral confining pressure was applied with three clamps and the performance of plain concrete cylinders was compared with that of steel-jacketed cylinders. Steel jacket proved to be effective in increasing the strength of the cylinder. Finally, Li's constitutive model was compared with that of the experimentalresults. However, Li's model showed incongruity in Region II, which indicates the region after the yield of steel jackets. Therefore, the modified value of n was used for the region and the model showed a good agreement.

Production of Biosurfactant by Pseudomonas sp. SW1 for Microbial Remediation of Oil Pollution (유류오염방제를 위한 Pseudomonas sp. SW1로부터 생물계면활성제의 생산)

  • Son, Hong-Joo;Suk, Wan-Su;Lee, Geon;Lee, Sang-Joon
    • Korean Journal of Microbiology
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    • v.33 no.3
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    • pp.193-198
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    • 1997
  • Microorganisms capable of producing biosurfactant were isolated from oil-contaminated soils and seawater. Among them, the selected strain SW1 was identified as Pseudomonas sp. by taxonomical characteristic tests, and so tentatively named Pseudomonas sp. SWI. The optimal temperature and initial pH for biosurfactant production were TEX>30^{\circ}C.$ and 7.0, respectively. The optimal medium composilion for the production of biosurfactant by Pseudomonas sp. SW1 were hexadecane of 2.0%, yeast extract of 0.04%, $K_{2}HPO_4$ of 0.02%, $KH_2PO_4$ of 0.03% and $MgSO_4$ center dot $7H_2O$ of 0.04%, respectively. Under the above conditions, minimum wrface tension was 32 mN/m after incubation of 2 days. The biosurfactant was produced during initial stationary phase in the optimal medium. Pseudotnonas sp. SWl utilized various hydrocarbons such as Bunker oils, n-alkanes and branched alkanes as a sole carbon source.

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Production of Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid by Pseudomonas sp. HJ (Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산)

  • 손홍주;민관필이상준
    • KSBB Journal
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    • v.10 no.4
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    • pp.349-356
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    • 1995
  • To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were $37^{\circ}C$ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

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Isolation and Characterization of a β-galactosidase Producing Thermophilic Bacterium (β-galactosidase를 생성하는 고온성(高溫性) 세균(細菌)의 생리적(生理的) 특성(特性)에 관한 연구(硏究))

  • Lee, Jong Soo;Kim, Chan Jo
    • Korean Journal of Agricultural Science
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    • v.9 no.1
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    • pp.377-386
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    • 1982
  • This experiment was carried out to elucidate the thermotolerant properties of a thermophilic bacterium which was isolated from soils of the hot springs area and selected for the ${\beta}$-galactosidase production. This bacterium was identified as a strain belong to the genus Thermus. Biochemical and physiological characteristics of this strain were studied, including the investigation of the fatty acid composition of its neutral fats. The results obtained were summarized as follows. 1. Optimal temperature and pH for growth of this strain were $65^{\circ}C$ and pH 6.5 respectively, and it was found to be an absolute thermophilic bacterium which could not grow at the temperature below $43^{\circ}C$. 2. No growth was obtained in the medium which contained more than 1.0% of sodium chloride. 3. The tolerable concentration of antibiotics were 10mg of penicillin G per ml of medium and 0.5mg of chloramphenicol per ml respectively. 4. This strain had auxotrophilic requirements for calcium-pantothenate and pyridoxin-HCl as an essential factor and for niacin as a stimulative factor. 5. Yellow pigment was released into the liquid culture of this strain, which showed maximum absorption at 420 nm. 6. Fatty acid composition of neutral fats of the strain was palmitic acid, 60.20%; lauric acid, 11.80%; myristic acid, 7.56%; behenic acid, 4.25%; capric acid, 1.77%; stearic acid, 2.13%; arachidic acid, 1.53%; and others unidentified, 10.7%.

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Isolation of a Lignolytic Bacterium for Degradation and Utilization of Lignocellulose (Lignocellulose의 분해 및 이용을 위한 Lignin 분해 세균의 분리)

  • 김용균;김한수;김근기;손홍주;이영근
    • Journal of Life Science
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    • v.12 no.4
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    • pp.392-398
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    • 2002
  • 38 strains were isolated in order to utilize lignin degrading ability from soil and compost. A organism having high lignin degrading ability of the isolated strains determined morphologcal and biochemical characteristics. Enrichment technique yielded a lignin degrading bacterium characterized as Pseudomonas sp. LC-2. This strain was able to degrade lignin which are the true representatives of native lignin and transform lignin to a lot of aromatic compounds as HPLC analysis of culture. By polyacrylamide gel analysis, it was determined that peroxidase consisted of three enzymes, with only one, the lignin peroxidase having high activity.

Isolation and Identification of Cellulose-Producing Bacteria (Microbial Cellulose 생산세균의 분리 및 동정)

  • 손홍주;이오미;김용균;이상준
    • Microbiology and Biotechnology Letters
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    • v.28 no.3
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    • pp.134-138
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    • 2000
  • Extensive screening for cellulose-producing bacteria was done using differential media. Fifty seven strains were isolated totally from the fruits and the vinegar, respectively; the isolate A9 strain from apples was selected and examined to determine its taxonomical characteristics. The bacterium was identified as the genus Acetobacter sp_ based on morphological, cultural and biochemical properties. A9 strain produced acetic acid from ethanol and decomposed acetic acid to $CO_2$ and $H_2O$. They produced dihydroxyacetone from glycerol but did not produce y-pyrone from glucose and fructose. When A9 strain was cultivated statically in Hestrin and Schramm liquid medium(HS medium). thick cellulose pellicle was formed_ Higher cellulose production was obtained in the shaken culture using HS medium at 100 rpm.

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Microbiological Studies on the Rice Makkulli (Part 1) Utilization of Rice Makkulli Koji with the Isolated Strain M-80 (쌀막걸리의 미생물학적 연구 (제1보) 분리균주 M-80의 쌀막걸리 제국용으로서의 이용성)

  • 조용학;성낙계;정덕화;윤한대
    • Microbiology and Biotechnology Letters
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    • v.7 no.4
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    • pp.217-223
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    • 1979
  • Six strains of mold which had high saccharifying and acid-productive ability were isolated from wild sources. The strain M-80 among them and Aspergillus kawachii, which was generally used as rice makkulli koji were used for this studies. The results obtained were summarized as follows. 1) $\alpha$-amylase activities of the strain M-80 and Asp, kawachii showed similarly as about 140W. V., while $\beta$-amylase activity of M-80 was 54 A. U. and Asp. kawachii was 40 A. U. 2) Acid protease activity of M-80 was higher then Asp. kawachii but alkaline protease activity was lower Asp. kawachii respectively. 3) The contents of total acid, ethanol and fusel oil in makkulli brewing with M-80 were higher those of Asp. kawachii and methanol contents of the tested two strains were about 33mg/%. 4) Fifteen kinds of free amino acid were detected from makkulli brewing of two strains, and free amino acid contents of M-80 were 10% higher than those of Asp. kawachii.

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