• Title/Summary/Keyword: 부착 실험

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THE EFFECTS OF CITRIC ACID TREATED ROOT SURFACES ON THE ATTACHMENT AND PROLIFERATION OF PERIDONTAL LIGAMENT CELLS (치근면 구연산 도포가 치주인대세포의 부착과 전개에 미치는 영향)

  • Lee, Sang-Gu;Suh, Jo-Young;Park, Joon-Bong
    • Journal of Periodontal and Implant Science
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    • v.23 no.1
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    • pp.77-96
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    • 1993
  • This in vitro study was undertaken to observe whether citric acid application aids the attachment and proliferation of human periodontal ligament cells to the root surfaces of periodontally diseased teeth. The roots were prepared so that the comparison could be made among the control healthy root surface, citric acid demineralized and non-demineralized root planted surfaces. Prior to the cell attachment experiment, each groups were prepared for scanning electron microscopic (SEM) examinations of root surface morphology, All specimens were fixed with phosphate buffered glutaraldehydes, postfixed with phosphate buffered osmium tetraoxide and stained with phosphate buffered tannic acid. dehydrated in ethanol, critical point dried, sputter coated with gold and examined under the SEM. In the cell attachement experiment, human cultured periodontal ligament cells at concentration to $4.5{\times}\;10^4\;cells/ml$ were seeded in each culture well which contained prepared roots and incubated for 30min 1, 2, 6, 12 and 24 hours at 37, 5% $CO_2$air incubator. Than the specimens were prepared for SEM examination using, the same methods as described above. In the cell proliferation experiment, $5{\times}\;10^4\;cells/ml$ cells were seeded incubated with the specimens for 6 hours. Then, all of the specimens were moved into fresh culture well and incubated for 24, 48, and 72 hours. The cell counting was done after trypsinization, under light microscope. The results were as follows. When viewed the surface morphology prior to the cell attachment, the non acid treated root planed surface displayed scaling striation and occasional bacteria and calculus. The citric acid treated specimens displayed little debris on the surface and funnel shaped orifices of dentinal tubules. There were no apparent differences in the morphology of cells attached to the control and experiment groups. However, in initial attachement, there was a slight more enhanced appearance in attachment in citric acid treated groups than other root surfaces. After 6 hours of incubation, most of the cells initiated the alteration of cell morphology from ovoid to spindle shapes. After 24 hours of incubation, most of the cells displayed proliferated appearance and connected with each other via numerous processes. In the cell proliferation experiments, there were statistically significant increased number of cells in citic acid treated groups than other groups.

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Bond Characteristics and Splitting Bond Stress on Steel Fiber Reinforced Reactive Powder Concrete (강섬유로 보강된 반응성 분체 콘크리트의 부착특성과 쪼갬인장강도)

  • Choi, Hyun-Ki;Bae, Baek-Il;Choi, Chang-Sik
    • Journal of the Korea Concrete Institute
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    • v.26 no.5
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    • pp.651-660
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    • 2014
  • Structural members using ultra high strength concrete which usually used with steel fiber is designed with guidelines based on several investigation of SF-RPC(steel fiber reinforced reactive powder concrete). However, there are not clear design method yet. Especially, SF-RPC member should be casted with steam(90 degree delicious) and members with SF-RPC usually used with precast members. Although the most important design parameter is development method between SF-RPC and steel reinforcement(rebar), there are no clear design method in the SF-RPC member design guidelines. There are many controversial problems on safety and economy. Therefore, in order to make design more optimum safe design, in this study, we investigated bond stress between steel rebar and SF-RPC according to test. Test results were compared with previously suggested analysis method. Test was carried out with direct pull out test using variables of compressive strength of concrete, concrete cover and inclusion ratio of steel fiber. According to test results, bond stress between steel rebar and SF-RPC increased with increase of compressive strength of concrete and concrete cover. Increasing rate of bond stress were decrease with increase of compressive strength of SF-RPC and concrete cover significantly. 1% volume fraction inclusion of steel fiber increase the bond stress between steel rebar and SF-RPC with two times but 2% volume fraction cannot affect the bond stress significantly. There are no exact or empirical equations for evaluation of SF-RPC bond stress. In order to make safe bond design of SF-RPC precast members, previously suggested analysis method for bond stress by Tepfers were evaluated. This method have shown good agreement with test results, especially for steel fiber reinforced RPC.

Evaluation of Beam Behavior with External Bonded L-type GFRP Plate through bending Test (L형 GFRP 외부부착 보강된 보의 휨 실험을 통한 보강 거동분석)

  • Jeong, Yeong-Seok;Kwon, Min-Ho;Kim, Jin-Sup;Nam, Gwang-sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.11
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    • pp.86-93
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    • 2020
  • The demand for maintenance in Korea infrastructure facilities constructed since the 1970s has increased significantly compared to the demand for new construction. Moreover, after the Gyeongju and Pohang earthquakes, seismic performance evaluation, repair, and retrofitting projects have been carried out. Therefore, in this study, a specimen was designed following the L-type GFRP Plate Externally Bonded Retrofit method, one among other retrofit methods. The L-type GFRP Plate was bonded to the specimen by epoxy and a washered steel nail. A four-point bending test was performed to confirm the strengthening effect of the Externally Bonded Retrofit method using an L-type GFRP Plate. The strengthening effect of the L-type GFRP plate was proven experimentally, and the behavior of the beam designed following the L-type GFRP Plate Externally Bonded Retrofit method was evaluated according to Korea's "Design Manual & Specification for Strengthening of RC Structures by Advanced Composites System". Furthermore, the effectiveness of the bonding method, a combination of epoxy and washered steel nail, was also checked. The results showed that the design, according to the guidelines mentioned above, predicted the strength of the member well, but the failure mode did not satisfy the design assumption because of unexpected damage to the GFRP plate due to the fixing method, washered steel nail.

A Prediction of the Long-Term Deflection of RC Beams Externally Bonded with CFRP and GFRP (CFRP와 GFRP로 외부 부착된 철근콘크리트보의 장기 처짐 예측)

  • Kim, Sung-Hu;Kim, Kwang-Soo;Han, Kyoung-Bong;Song, Seul-Ki;Park, Sun-Kyu
    • Journal of the Korea Concrete Institute
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    • v.20 no.6
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    • pp.765-772
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    • 2008
  • For RC structures, long-term deformation occurs due to the inherent characteristics, which are creep and shrinkage. In terms of serviceability, it is important to limit deflection caused by the deformation to the allowable deflection. In the recent years, various repair and strengthening methods have been used to improve performance of the existing RC structures. One of the typical methods is FRP externally bonded method (EBR). Fiber reinforced polymer (FRP) has been used worldwide as repair and strengthening materials due to its superior properties. Besides, it has to offer improved strengthening performance not only under instantaneous load but sustained load. Therefore, accurate prediction method of deflection for the RC members externally bonded with FRP under sustained load is required. In this paper, three beams were fabricated. Two beams were externally strengthened with one of CFRP plate and GFRP plate respectively. Total three beams were superimposed under sustained load of 25 kN. During 470 days, deflections at midspan were obtained. Moreover, creep coefficients and shrinkage strains were calculated by using ACI-209 code and CEB-FIP code. In order to predict the deflection of the beams, EMM, AEMM, Branson's method and Mayer's method were used. Through the experiment, it was found that the specimen with CFRP plate has the most flexural capacity and Mayer's method is the most precise method to predict total long-term deflections.

Structural Behavior of 3D Printed Concrete Specimens with Reinforcement (보강재가 있는 3D 프린팅 콘크리트의 구조거동)

  • Joh, Changbin;Lee, Jungwoo;Yang, In-Hwan
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.3
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    • pp.174-181
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    • 2018
  • This paper examines the structural behavior of 3D printed concrete specimens with focus on the bond between the layers. The tensile bond and flexural strengths were investigated experimentally and compared with those of specimens made by conventional mold casting. The test parameters were the time gap between printing layers and the reinforcement between vertical layers. The results showed the 3D printed specimens had voids between layers and confirmed the strength reduction due to printing time gap and the stress concentration caused by the voids. Most of the reduction in tensile bond strength between layers was due to the stress concentration at least up to certain printing time gap. Moreover, beyond a certain printing time gap (24hours), the additional reduction in tensile bond strength reached a level that could affect the structural behavior. The reinforcement between layers was helpful to increase the ductile behavior which is essential to prevent the sudden collapse of the structure. In addition, the reduction in flexural strength due to the stress concentration by the voids was observed and should be considered in the design of 3D printed wall structures against the lateral load.

Evaluating Joint Motion Sensing Efficiency According to the Implementation Method of CNT-Based Fabric Sensors (CNT 기반의 직물센서 구현 방법에 따른 관절동작 센싱 효율 평가)

  • Cho, Hyun-Seung;Yang, Jin-Hee;Lee, Joo-Hyeon
    • Science of Emotion and Sensibility
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    • v.24 no.4
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    • pp.129-138
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    • 2021
  • This study aimed to determine the effects of the shape and attachment position of stretchable textile sensors coated with carbon nanotube on their performance when used to measure children's joint movements. Moreover, the child-safe requirements for fabric motion sensors are established. The child participants were advised to wear integrated clothing equipped with the sensors of various shapes (rectangular and boat-shaped) and attachment positions (at the knee and elbow joints or 4 cm below the joints). The voltage change induced by the elongation and contraction of the fabric sensors was determined for arm and leg flexion-extension motions at 60 deg/s (three measurements of 10 repeats each for 60°and 90°angles, for a total of 60 repetitions). Their dependability was determined by comparing the fabric motion sensors to the associated acceleration sensors. The experimental results indicate that the rectangular-shaped sensor affixed 4 cm below the joint is the most effective fabric motion sensor for measuring children's arm and leg motions. In this study, we designed a textile sensor capable of tracking children's joint motion and analyzed the sensor shape and attachment position on motion sensing clothing. We demonstrated that flexible fabric sensors integrated into garments may be used to detect the joint motions of the human body.