• Title/Summary/Keyword: 부착작용

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Surface Modification of Biomaterials for Hard Tissue Substitutes to Improve Biocompatibility and Osteoconductivity (생체적합성 및 골전도성 향상을 위한 경조직 대체용 생체재료의 표면개질)

  • Kim, Sung-Wook;Lee, Woo-Kul
    • Applied Chemistry for Engineering
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    • v.16 no.6
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    • pp.725-730
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    • 2005
  • In the development of biomaterials as a substitute of hard tissues, the biocompatibility and osteoconductivity of the biomaterial are considered to be one of the most significant considerations. These biological properties of a material can be greatly improved by the modification of the surface properties by the depositing calcium phosphate thin films on the material since calcium phosphate films possess similar chemical compositions to hard tissues. The success of a material as a biomaterial will be determined by the interaction of the surface of the material with the adhesion molecules which induce cellular adhesion and biological responses of the adherent cells. Depending on the adsorption mechanisms and adsorbed conformation of the adhesion molecules on the surface of the biomaterial, cellular responses, such as adhesion, proliferation and differentiation of osteoblast cells, can be promoted or restricted. It has been reported that materials of which surfaces were modified with thin films of calcium phosphate appeared to be more osteoconductive. Rapid formations of bone nodule in addition to higher differentiations of osteoblast have been observed on the calcium phosphate thin films.

Assessment of cell adhesion, cell surface hydrophobicity, autoaggregation, and lipopolysaccharide-binding properties of live and heat-killed Lactobacillus acidophilus CBT LA1 (락토바실러스 아시도필러스 CBT LA1 생균과 사균체의 세포부착력, 자가응집력, 소수성 상호작용력, LPS 결합력에 대한 평가)

  • Shin, Joo-Hyun;Lee, Joong-Su;Seo, Jae-Gu
    • Korean Journal of Microbiology
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    • v.51 no.3
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    • pp.241-248
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    • 2015
  • Although studies on probiotics have been performed mostly with viable microbes, the beneficial functions of dead or heat-killed form of probiotic strains have also been examined. In this study, live and heat-killed forms of Lactobacillus acidophilus CBT LA1 were investigated in vitro and in vivo to evaluate the properties necessary for gut barrier protection. Cell surface hydrophobicity (CSH), autoaggregation (AA), cell adhesion, and lipopolysaccharide (LPS)-binding properties were evaluated. In addition, the suppressive effect on LPS-induced interleukin (IL)-8 expression was investigated in HT-29 cells. To identify optimal conditions for CBT LA1 to adhere to HT-29 cells, CBT LA1 cells were heat-treated at 80, 85, 90, 95, 100, or $121^{\circ}C$ for 10 min; cells treated at $80^{\circ}C$ for 10 min showed the highest adhesion. Heat-killed bacteria at $80^{\circ}C$ showed higher levels of LPS-binding, CSH, AA, adhesion to HT-29, and suppression of IL-8 expression than did live CBT LA1. In vivo imaging was performed to evaluate the ability of live or heat-killed CBT LA1 to remove LPS from the intestine in a rat model of infection. At 16 h after infection, fluorescence from FITC-conjugated LPS had mostly disappeared from the intestine of the rats administered with live or heat-killed CBT LA1; the effect was greater with heat-killed CBT LA1 at $80^{\circ}C$. These results suggest that heat-killed CBT LA1 as well as its live form can be applied as a pharmabiotic for protection of the gut barrier.

Bond Properties of GFRP Rebar with Cover Thickness and Volume Fraction of Steel Fiber (강섬유 혼입률과 피복두께에 따른 GFRP 보강근의 부착특성)

  • Choi, Yun-Chul
    • Journal of the Korea Concrete Institute
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    • v.24 no.6
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    • pp.761-768
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    • 2012
  • The purpose of this study is to investigate bond properties of GFRP used in SFRC (Steel fiber reinforced concrete) and normal concrete. The experimental variables were rebar diameter (D13, D16), steel fiber volume fraction (0~2%) and cover thickness ($1.5d_b$, $5.4d_b$). The experimental results showed a different failure mode depending on the cover thickness. Through the tested specimens, splitting failure occurred for the specimens with small cover thickness and pull out failure occurred in the specimens with large cover thickness. Introduction of steel fiber caused the specimens to have more ductile behavior of bond stresss-lip after peak stress, but they did not increase the bond strength significantly. These failure modes were shown in both steel reinforcement and GFRP. However, from the difference of micro structure of bond failure mechanism between steel rebar and GFRP rebar, more ductile behavior was observed in GFRP-specimens after maximum bond strength was reached.

Deposit Amounts of Dithianone on Citrus leaves by Different Spray Methods (살포 방법에 의한 살균제 Dithianon의 감귤 잎 부착량 비교)

  • Jeon, Hye-Won;Hong, Su-Myeong;Hyun, Jae-Wook;Hwang, Rok-Yeon;Kwon, Hye-Young;Kim, Taek-Kyum;Cho, Nam-Jun
    • The Korean Journal of Pesticide Science
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    • v.20 no.1
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    • pp.1-6
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    • 2016
  • In order to obtain efficient control effect of the pesticide, it is important to ensure uniform adhesion to the desired plant parts at the right time. Pesticide spray method (application technology) is an important factor affecting the efficacy and crops persistent expression. The aim of this study was to develop an efficient system to investigate the coating weight distribution of citrus leaves due to the difference between the nozzle and spray sprinkler system using dithianon used in citrus scab. Other An, engine type sprayer was used as the control. Speed sprayer and different sprinklers were wsed to way the deposit amounts of dithianon on citrus leaves. The test was conducted at the National Institute of Horticultural Herbal Science Citrus Research Station, located in the circle citrus Jeju Island. In order to examine whether the citrus orchard spray and the evenl on the whole, dithianon (43% flowable 1000-fold dilution) was sprayed, filter paper and leaves were analyzed by the height as top, middle, bottom. Speed sprayer the was most effective on depositing at the middle position, of the leaves. All other sprays the leaces except the dry mist sprinkler were not effective enough to deposit on the back sides. To achieve more deposits on the high position leaves, an improve ment in the nozzle and an efficient power system of sprayer were needed.

The Effect of Ice Adhesion according to Functional Group and Chemical Structure of Additive (화합물 작용기와 화학구조에 따른 수용액의 빙부착 억제 효과)

  • Chung, Dong-Yeol;Peck, Jong-Hyeon;Kang, Chae-Dong;Hong, Hi-Ki
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.8
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    • pp.607-614
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    • 2007
  • This paper investigated that the functional group and chemical structure of additives affect ice adhesion in aqueous solutions cooling with stirring. In order to compare the effect on the ice adhesion in aqueous solutions, the functional group like carboxyl (-COOH), hydroxyl(-OH) or amine($-NH_{2}$) one were compared each other. Among the functional group, the strength of the hydrogen bonding force order is amine, hydroxyl and carboxyl one. It supports that ethylene diamine 7 mass% solution including amine group was effective to suppress the ice adhesion, though it is corrosive. Also, the ice adhesion were effectively resisted and formed lots of ice slurries in cooling experiment of 7 mass% solution of 1, 2-and 1, 3-propanediol which is different molecular structure but equal molecular weight each other.

Grammatical Relation Analysis using Support Vector Machine in BioText (바이오 문서에서 지지 벡터 기계를 이용한 문법관계 분석)

  • Park, Kyung-Mi;Hwang, Young-Sook;Rim, Hae-Chang
    • Annual Conference on Human and Language Technology
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    • 2003.10d
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    • pp.287-292
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    • 2003
  • 동사와 기본구 사이의 문법관계 분석은 품사부착과 기본구 인식이 수행된 상태에서, 동사와 의존관계를 갖는 기본구를 찾고 각 구의 구문적, 의미적 역할을 나타내는 기능태그를 인식하는 작업이다. 본 논문에서는 바이오 문서에서 단백질과 단백질, 유전자와 유전자 사이의 상호작용관계를 자동으로 추출하기 위해서 제안한 문법관계 분석 방법을 적용하고 따라서 동사와 명사고, 전치사고, 종속 접속사의 관계만을 분석하며 기능태그도 정보추출에 유용한 주어, 목적어를 나타내는 태그들로 제한하였다. 기능태그 부착과 의존관계 분석을 통합해 수행하였으며, 지도학습 방법 중 분류문제에서 좋은 성능을 보이는 지지 벡터 기계를 분류기로 사용하였고, 메모리 기반 학습을 사용하여 자질을 추출하였으며, 자료부족문제를 완화하기 위해서 저빈도 단어는 품사 타입 또는 워드넷의 최상위 클래스의 개념을 이용해서 대체하였다. 시험 결과지지 벡터 기계를 이용한 문법관계 분석은 실제 적용시 빠른 수행시간과 적은 메모리 사용으로 상호작용관계 추출에서 효율적으로 사용될 수 있음을 보였다.

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괘선/구부림 강공과 종이물성과의 상관관계 II

  • 조신환;오홍석;최대웅;여성국
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2001.11a
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    • pp.203-203
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    • 2001
  • 패선/구부림 가공은 후가공의 일부분으로서. 박스가공 시 종이에 패션치기와 구부림 공정이 들어가게 되며 이때 종이표면이 약할 경우 금이가는 현상이 발생한다. 본 연구 는 이러한 가공과 다층판지의 물성이 패선/구부림 적성에 영향을 미치는가에 대한 연 구로서 2000년도 추계논문발표시 종이 물성 중 패션/구부림 가공에 영향을 주는 것으 로 표면층의 층간결합력과 내절도 그리고 인장강도가 중요한 변수로 작용하는 결과를 얻었으며 이후 이들 영향인자 중 핵심영향인자의 도출을 위하여 침엽수를 배합하지 않 은 상태에서 상질고지 재생펄프를 고해 처리하여 특성을 향상시켜 실제 공정에서 실험 하였다. 1차 실험은 공정실험 전 예비 실험으로서 재생펄프를 공장 refiner를 사용하여 고해 처리하여 강도향상 효과를 분석하였다. 분석결과 인장강도는 증가하나 내절도 향상은 기대에 미치지 못하는 것으로 나타났다. 이에 따라 1차 공정실험 조건을 부착량을 향상 시켜 인장강도를 침엽수 펄프 배합수준으로 끌어올리는 방법을 선택하였으며 그 결과 패선/구부림 적성이 기존대비 양호한 결과를 얻었다. 즉 인장강도 향상으로 침엽수 펄 프를 대체할 수 있다는 결론을 얻었다. 2차 실험은 부착량 대선 refiner를 사용하여 상 질고지 재생펄프를 고해 처리하여 인장강도를 향상시켜 생산하였다. 실험결과 인장강도 가 침엽수펄프 배합 시 대비 동등이상 수준에 있을 경우 패션/구부린 가공 시 금이가 는 터짐문제는 발생하지 않는 것으로 나타났다. 3차 실험은 두 차례 동안 실시된 공정 실험을 기준으로 refining 처리와 부착량 기준을 설정하고 장시간 생산하여 그 특성을 측정하였다. 실험결과 표면층의 인장강도가 낮아질 때 패선/구부림 적성이 약해지는 경 향을 보였으며 인장강도가 기존 침엽수펄프 배합대비 동등이상일 경우 패선/구부림 가 공적성이 양호하게 나타났으며 실제 가공업체에서도 터짐 문제가 발견되지 않았다. 결론적으로 표면층의 인장강도가 패션/구부림에 가장 중요한 변수로 작용하며 어떠 한 형태로 표면층의 인장강도를 향상시킬 경우 침엽수 펄프는 재생펄프로 대체가 가능 할 것으로 판단된다.

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Bacteriological Control of Cyanobacterial Bloom. (시안세균 수화의 세균학적 조절)

  • 김철호;권오섭;이진애
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.115-120
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    • 2004
  • A Gram (-), rod-shaped bacterium in size of 1.3∼$1.8{\times}0.35{\mu}m$ inhibiting the growth of cyanobacterium (Ana-baena cylindrica) was isolated and designated NG-2 in this manuscript. This isolate showed positive reactions for catalase and oxidase, and optimal growth conditions of 35∼TEX>$40<^{\circ}C$ and pH 9.0. In a mixed-culture of A. cylindrica and the isolate, each microorganism grew inverse-proportionally, and the cyanobacterial vegetative cells almost completely disappeared within 24 hours. NG-2 lysed A. cylindrica only under light, which means that lytic activity of NG-2 was dependent on the photosynthetic activity of host. When observed under phase contrast microscope, the isolate lysed vegetative cells of A. cylindrica in scattered state in a liquid medium, whereas het-erocysts have not been lysed. When cyanobacterial cell walls have been lysed partly, NG-2 attatched around A. cylindrica filament and formed colony, then encouraged complete lysis of cyanobacterial cells. The isolate showed similar lytic activity in natural water as in an artificial medium. And lytic activity of NG-2 was enhanced when attached on expandable polystyrene bead.

Strength Development Properties of Latex Modified Concrete For New Concrete Bridge Deck Overlay (신설 콘크리트 교면 덧씌우기를 위한 라텍스 개질 콘크리트의 강도발현 특성)

  • Yun, Kyong-Ku;Kim, Ki-Heoun;Lee, Joo-Hyung;Hong, Chang-Woo;Kim, Dong-Ho
    • International Journal of Highway Engineering
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    • v.3 no.3 s.9
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    • pp.135-146
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    • 2001
  • This study focused on the investigation of compressive and flexural strengths development, and bond strength of latex modified concrete in order to validate the feasibility of application into concrete bridge deck overlay. Pull-out bond test was used for evaluating the bond strength of latex modified concrete to substrate. The main experimental variables were latex-cement ratio, surface preparation and moisture levels. The compressive strength of latex modified concrete decreased slightly and the flexural strength increased as the latex content increased from 5% to 20%. This might be due to the flexibility latex filled in voids and interconnections of hydrated cement and aggregates by a film of latex particles, respectively. In general, increasing the amount of latex will produce concrete with increased tensile and flexural strength and lower modulus of elasticity. Significant improvements in bond strength between new and existing concrete were achieved through the modification of the new concrete bridge deck overlay by latex polymers. The effect of surface preparation on bond of latex modified concrete to conventional concrete were significant at the conditions by sand paper and wire brush. A better bond could be achieved by rough surface rather than smooth. The saturated condition of surface is the most appropriate moisture level among the considered followed by dry condition and wet condition.

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Pullout Characteristics of Geogrid with Attached Passive Reinforcement (마찰돌기를 부착한 지오그리드의 인발특성 평가)

  • Moon, Hongduk;Yoo, Chulho
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.11
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    • pp.43-51
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    • 2014
  • In this study, a series of pullout experiments were conducted on geogrid with attached passive reinforcement with respect to silt containments. Experiments were performed on man-made sand ground containing different silt of 0 %, 17 %, 35 % under various normal stresses 30 kPa, 60 kPa, 120 kPa respectively. The pullout test results showed that passive reinforcement increased the pullout strength over all silt contained condition and showed up to 20 % increases for same soil condition. The test results converted to the coefficient of interaction of pullout test to investigate the effect of reinforcement and the case of passive reinforcement showed 0.7~1.6 distribution depend on a silt contents. Therefore it is concluded that the overall length of geogrid can be reduced under the low vertical stress conditions.