• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.1-16
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• 2006

Purpose : The purpose of this research was to investigate the effects of Kamibojoongikkitang (KBT) on the immune cells in C57BL/6 mice. Methods : KBT (500mg/kg) was administerd p.o. once a day for 7 days. Results : KBT decreased the cell viability of thymocytes in vivo and in vitro system and decreased the cell viability of splenocytes in vivo, but increased the viability of splenocytes in vitro system. In addition, KBT did not affect the population of helper T (Th) cells and cytotoxic T (Tc) cells in thymocytes and decreased the population of T- and B-lymphocytes and the population of Th and Tc cells in splenocytes. Furthermore, KBT did not affect the production of ${\gamma}%-interferon and interleukin-4 in splenocytes. KBT increased the production of nitric oxide in vivo but decreased the production of nitric oxide in vitro system. KBT enhanced the phagocytic activity of peritoneal macrophages in vivo, but decreased the phagocytic activity in vitro. Conclusion : KBT has an inhibitory action on the specific immune response via decrease of the cell viability of thymocytes and splenocytes and has a potent action on the non-specific immunity via increase of phagocytic activity of peritoneal macrophages.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.77-92
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• 2006

Purpose : This study is to examine what are the effects of the Woogwiyeum(WGY) on the ovariectomized rat model of postmenopausal osteoporosis. Methods : We devide mice to 3 Group(Sham operated group, control group, WGY treated group) and analyze each serum component. Results : 1. Body weight in control group showed significant increase in comparison with sham, but that in WGY-treated showed no change in comparison with control. 2. The level of serum albumin, ALP in control group showed significant decrease in comparison with sham. That in WGY-treated was decreased in comparison with control. 3. Trabecular bone area as well as trabecular thickness in control group showed significant decrease in comparison with sham. Those in WGY-treated showed significant increase in comparison with control. 4. Osteoclast number and oseoblast surface in control group showed significant increase in comparison with sham. Those in WGY-treated showed significant decrease in comparison with control. Conclusion : WGY has shown to be capable of preventing and curing osteoporosis caused by old-aged and postmenopause.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.148-158
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• 2006

Purpose : Sanhupung(産後風) is not completed golden standards of diagnosis therefore doctors diagnose through symptoms and signs of patients in clinic same as progress of treatments. We polled oriental gynecologic specialists and non-specialisst to find out cognitive differences on Sanhupung. Subjects : We surveyed 83 specialists and 87 non-speciailist from May 15 2005 to Oct 15. We analyzed the result of respondants, 39 (47%) specialists and 65 (75%) nono-specialits). Methods : We sorted all symptoms in previous studies then we categorized them into 4 fields. The results are analyzed by frequency, importance and impact(frequency percentage${\times}$average importance). Results : Except back pain, all pain symptoms were lower evaluated in non-specialist on frequency. Except inguinal region pain, pain of lower limbs and back pain, all pain symptoms were lower evaluated in non-specialist on importance. Except the numbness of limbs, all items of whole body were lower evaluated in non-specialist on frequency. Except edema, numbness of hand and foot and discharge from the uterus, all items were lower evaluated in non-specialist on importance. All items of neuropsychotic symptoms were lower evaluated in non-specialist on frequency and importance.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.159-173
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• 2006

Purpose : The purpose of this study is to find out relation between DSOM scores and an attack of uterus myoma in oriental medicine. Methods : We analyze the medical records for 386 patients including 257 uterus myoma patients who visited Dongeui University Oriental Medical Center from May 2001 to June 2006. We investigate the DOSM symptom scores which effect uterus myoma patients using logistic regression model. Results : Logistic regression analysis indicate that 14 items of DSOM were associated with the myoma, and the results show that correct rate is equal to 79.79%, sensitivity is 83.66%, specificity is 72.09%. Conclusion : There is fair relation between DSOM scores(14 items of DSOM) and an attack of uterus myoma in oriental medicine.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.93-116
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• 2006

Purpose : This study is to examine the effects of Hwaganjeon water extract(HGJ) on immobilization-stress or cold-stress in BALB/C mice. Methods : We have Hwaganjeon water extract(HGJ) by freeze-dryer & melt it by a saline solution. We feed HGJ 500mg/Kg to 5mice, and add immobilization-stress by putting mice in plastic cylinder 10 hours, and add cold-stress by putting mice in $4^{\circ}C$ cold room 6 hours. Results : 1. HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells at the concentration of 1 mg/ml. 2. HGJ did not affect the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but increased the cell viability of splenocytes decreased by immobilization-stress or cold-stress. 3. HGJ decreased the population of splenic $CD4^{+}$ and $CD8^{+}$cells increased by immobolization-stress or cold-stress. 4. HGJ enhanced the production of ${\gamma}$-interferon(IFN) and interleukin(IL)-2 decreased by immobilization-stress or cold-stress. Conclusion : These results indicate that HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.17-32
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• 2006

Purpose : The aim of this study is to investigate the antibiotic effects of 14 herbs among blood-activating stasis-dispelling medicinals on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans, and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal normal flora. The blood-activating stasis-dispelling medicinals, Mucunae Caulis, Salviae Radix, Persicae Semen, Myrrha, Zedoariae Rhizoma, Achuranthis Radix, Leonuri Herba, Melandrii Herba, Gleditsiae Spina, Lycopi Herba, Scirpi Rhizoma, Caesalpiniae Lignum, Corydlais Tuber and Polygoni Cuspidati Radix were used in this study. In vitro antibiotic activities were observed by optical density and colony test. Results : The optical density and colony test showed that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals had antimicrobial effect. Gleditsiae Spina had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis and MRSA. Scirpi Rhizoma had antimicrobial susceptibility and selective toxicity in Staphylococcus aureus and MRSA. Corydlais Tuber had antimicrobial susceptibility and selective toxicity in MRSA. Polygoni Cuspidati Radix had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis, Staphylococcus aureus and MRSA. Melandrii Herba had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.225-244
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• 2006

Purpose : This study was performed to assess the clinical effect of Daejo-hwan(DJH) on climacteric syndrome, not only common symptoms such as hot flushes, anxiety, palpitation and so on, but also urogenital tract disturbances like vaginal dryness and sexual problems. Methods : We randomly divided 120 women with the climacteric syndrome into two groups. One group received only DJH 2 pills(4g) a time, three times a day for 8 weeks and the other group didn't get any treatment for the same period. We evaluated sonography, DXA, blood test, female hormone test and questionnaires in the beginning as well as after 8 weeks of the treatment. Symptoms were measured by Kupperman's index, Menopausal Rating Scale and the Greene Climacteric Scale. Results : In this study, DJH was innoxious on liver and kidney. And DJH in the treated group reduced climacteric symptoms significantly as compared to the control group without enlargement of uterus myoma and the change of estradiol. In Kupperman's index and MRS, DJH in the treated group especially had effects on fatigue, shoulder pain. anxiety, headache, hot flushes as well as sexual problems. Conclusion : These results suggest that DJH can be useful in treating various climacteric symptoms including sexual problems without influences on the size of uterus myoma and estradiol.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.47-60
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Scutellaria barbata D. D on on the cell proliferation of HeLa Cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Scutellaria barbata D. D on solution for 24, 48 and 72 hours for the direct inhibitory effects of Scutellaria barbata D. D on. Then we examined the effect of Scutellaria barbata D. D on solution on the cell proliferation inhibition by XTT assay. DNA fragmentation, MAP kinase activity and caspase activity by FACS analysis in HeLa cells. Results : We found that the proliferation of HeLa cells was significantly decreased in Scutellaria barbata D. D on solution containing groups comparing with a control group in a concentration-dependant manner. When HeLa cells were cultivated for 24 hours with 5% Scutellaria barbata D. D on solution containing group, the percentage of HeLa cells with activated caspase was the highest. Scutellaria barbata D. D on solution reduced the MAP kinase activity of HeLa cells comparing with the control group. By the XTT assay, the cell's activity was decreased in 5% and 10% Scutellaria barbata D. D on solution containing groups in 24 and 72 hours cultivation and 10% group in 48 hours. DNA fragmentation and caspase-3 activity of HeLa cells, however, were changed insignificantly. Conclusion : From this study we could suggest that Scutellaria barbata D. D on is available to the inhibition and apoptosis of human cervical carcinoma cell line, HeLa cells in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.33-46
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• 2006

Purpose : This study was conducted to investigate effects of 7 herbs among medicinals resolving dampness with aroma on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin- resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal flora. Medicinals resolving dampness with aroma, Pogostemonis Herba, Amomi Cardamomi Fructus, Amomi Semen, Atractylodis Rhizoma, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen and Magnoliae Cortex were used in this study. In vitro antimicrobial activities were observed by optical density and colony test. Results : The optical density showed that Alpiniae Katsumadaii Semen and Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on pathogenic vaginal microorganisms (Methicillin-resistant Staphylococcus aureus). Pogostemonis Herba, Ammomi Tsao-ko Fructus had antimicrobial effects on Gardnerella vaginalis. The colony test showed that Alpiniae Katsumadaii Semen, Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effects on Methicillin-resistant Staphylococcus aureus. Pogostemonis Herbs, Alpiniae Katsumadaii Semen had antimicrobial effects aganist Gardnerella vaginalis. The optical density showed that Magnoliae Cortex among medicinals resolving dampness with aroma had antimicrobial effect on normal vaginal microorganisms (Streptococcus spp.). The colony test showed that medicinals resolving dampness with aroma had no antimicrobial effect on normal vaginal microorganisms. Conclusion : From this study, we could suggest that Pogostemonis Herba, Ammomi Tsao-ko Fructus, Alpiniae Katsumadaii Semen, Magnoliae Cortex of medicinals resolving dampness with aroma are available to antimicrobial agent of pathogenic vaginal microorganisms in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.279-286
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• 2006

Purpose : The purpose of this study is to report the effect of oriental treatments to hyperemesis gravidarum in disturbed liver function. Methods : Hyperemesis gravidarum is a severe and intractable form of nausea and vomiting in pregnancy. It is a diagnosis of exclusion and may result in weight loss; nutritional deficiencies; and abnormalities in fluids, electrolyte levels, and acid-base balance. The peak incidence is at 4, 8-16 weeks of pregnancy. Interestingly, nausea and vomiting of pregnancy is generally associated with a lower rate of miscarriage. However disturbed diver dunction with Hyperemesis gravidarium is rare very and dangerous. Traditionally, oriental medical therapy has been used to patients with hyperemesis gravidarum and showed effective result. We treated 1 patient who had hyperemesis gravidarum in disturbed diver dunction at Se-Myung university Oriental Hospital in affiliation by Herbal medication, acupuncture and moxa therapy and got good result from them. Results : As a result, symptoms are remarkably alleviated and liver function test are improved. Conclusion : Therefore we would like to report that it is effective to cure the hyperemesis gravidarum in disturbed liver function through the herb-medication and acupuncture treatment.