• 보존과학연구
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• s.32
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• pp.171-183
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• 2011

In this study molecular genetic analysis was carried out on 4 human skeletal remains from Osuri, Buyeo. We showed that real-time PCR is the method of the choice to assess the initial number of genuine ancient DNA molecules. Human mitochondrial DNA quantification was accomplished by the real-time PCR for the cytochrome b gene of the mitochondria. Histological results proved to be a good potentiality for biochemical analysis using biomolecule. The level of specimen's preservation state was proved that level of quantitative result was BO-04, BO-01, BO-03, BO-02. Continually, we showed that biochemical and biomolecule results for the level of preservation state were similar. This study will be useful to important material for predicting biochemistry and biology analysis of the ancient bone.

• Korean Journal of Heritage: History & Science
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• v.47 no.4
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• pp.92-105
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• 2014

Microbial characteristics of bacterial population were investigated in human remains and soil inside the bones in excavated grave no.4 and no.5 at Keundokgol site, Osu-ri, Buyeo. Phylogenetic characteristics of bacterial populations were analyzed by direct extracting of ancient DNA. In this study, based on the 16S rDNA sequences, in case of grave no.4, 319s from human remain were classified into 11 phyla, and 462s from soil were classified into 16 phyla. In case of grave no.5, 271s from human remain were classified into 10 phyla, and 497s from soil were classified into 11 phyla. Especially, Actinobacteria phylogenetic group are dominant group of bacterial populations in grave no.4 and no.5. Also, most of these were analyzed uncultured group. Thus, the discovery of a diversely microbial community and uncultured group was thought to be due to the specificity of the sample. Conclusively the general excavated human bones were contaminated with soil bacteria species their near around. This results contribute to preservation and management of ancient human bone from archaeological sites.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.10 no.2
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• pp.43-54
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• 2000

C. Gamage 등은 M. Mambo 의 대리 서명 방시과 Y. Zheng의 signcryption 방식을 이용하여 사용자가 상대 적으로 계산 능력이 뛰어난 서버에 의존하여 암호화 및 서명을 생성할 수 있는 proxy-signcryption 방식을 제안하였다 그러나 그들이 제안한 방식을 실제 응용에 적용할 경우 사용자가 proxy agent를 대신하여 정당한 proxy-signcryption을 생성할 수 있을 뿐만아니라 자신이 전송한 메시지에 대해 부인하는 경우 리를 판단할 수 없으므로 proxy agent를 보호 할 수 없다는 문제점이 있다 따라서 본 논문에서는 대리인 보호형 대리 서명 방식과 N. Asokan 의 S3(Server Supported Signnatures)를 이용하여 proxy agent를 보호할 수 있고 송신자 부인 봉쇄를 제공하여 실제 응용에 적용할 수 있는 proxy-signcryption 방식을 제안하고자 한다. 또한 본 논문에서 제안하는 proxy-signcryption 방식은 한국형 디지털 서명 표준안인 KCDSA(Korean Certificate-based Digital Signature Algorithm)를 이용한다.