• Pediatric Infection and Vaccine
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• v.25 no.1
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• pp.35-44
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• 2018

Purpose: This study aimed to evaluate the immunogenicity and safety of a trivalent inactivated influenza vaccine (TIV) among healthy Korean children and adolescents. Methods: From October to December 2008, 65 healthy patients aged 6 months to 18 years who visited Korea University Ansan Hospital for influenza vaccination were enrolled in this study. We measured the hemagglutinin inhibition antibody titers at baseline and 30 days after vaccinating enrollees with split influenza vaccine and calculated the seroprotection rates, geometric mean titers, and seroconversion rates. Local and systemic adverse events were assessed after vaccination. Results: The seroprotection rates against all three viral strains (A/H1N1, A/H3N2, B) were 87.7%, 89.2%, and 89.2% (${\geq}70%$), respectively; seroconversion rates were 44.6%, 73.8%, and 63.1% (${\geq}40%$), respectively; and seroconversion factors were 4.5, 8.4, and 10.5 (>2.5), respectively. The TIV immunogenicity was acceptable according to the CPMP (Committee for Proprietary Medicinal Products) criteria. Although 48 patients (73.8%) reported one or more adverse events, no severe adverse events such as anaphylaxis and convulsion were observed. Forty-two patients (64.6%) reported a local skin reaction, including redness (29.2%), pain (43.1%), or swelling (41.5%) of the injected site, and 26 (40.0%) reported a systemic reaction: fatigue (23.1%), myalgia (20.0%), headache (10.8%), arthralgia (10.8%), chills (9.2%), or fever (7.7%). Conclusions: This study shows that the immunogenicity of the TIV vaccine is acceptable. As there were no serious adverse events aside from local reactions and mild systemic reactions, this vaccine can be safely used among healthy Korean children and adolescents.

• Journal of Life Science
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• v.17 no.12
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• pp.1660-1668
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• 2007

The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.386-395
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• 2007

A total of 27 strains of Vibrio parahaemolyticus (18 strains isolated from Korea and 9 strains from Japan) were serotyped and examined for biochemical characteristics, antimicrobial susceptibility patterns, cytotoxicity assay, thermostable direct hemolysin (TDH) production and molecular epidemiology. Using polymerase chain reaction (PCR) method and DNA probe hybridization method, the strains were tested for toxR, tdh, trh and ORF 8 genes. The V. parahaemolyticus isolated from patients were belonged to 8 different serotypes : O3:K6, O1:K38, O3:K57, O4:K9, O4:Kl2, O4:K68, O5:Kl5 and O6:K46. Urease-positive strain possessed the trh gene, and conversely, urease-negative strains lacked the gene, indicating that urease production by V. parahemolyticus strains strongly correlates with the possession of the trh gene. Most strains showed multiple resistant to more than three antibiotics and the antibiogram could be classified into 6 group (I to VI). All of the O3:K6 strains isolated in South Korea and Japan producted TDH at high levels. The TDH titers ranged between 256 and 2.048, and the average titer was 1009. To distinguish the new and increasingly common V. parahaemolyticus strains from clinical isolates, ORF 8 is a useful genetic marker. After Southern hybridization, the HindIII restriction fragment patterns of the tdh gene were grouped one type, respectively. One type showed two bands one of which was 4.3kb and the other was 11.5kb in size. Variation between the O3:K6 serotype are minor when compared to the differences seen with the non O3:K6 strains. The migration patterns of Not I -digested of the total DNA of the O3:K6 strains were similar, and only slight variations were observed between the serotypes. By contrast, the O3:K6 strains and non O3:K6 had markedly different profiles. In conclusion, Random amplified polymorphic DNA (RAPD) profile using appropriate primers was an effective epidemiological marker.

• Journal of Life Science
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• v.18 no.4
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• pp.515-521
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• 2008

This experiment was conducted to investigate effects of non-ionic or zwitterionic (+/-) surfactants on digestibility of rice straw, and changes of growth of rumen mixed microbes, pH, and gas production during in vitro fermentation. Also, during in vitro ruminal fermentation, microbial attachment on rice straw was investigated using scanning electron microscopy (SEM). Tween 80 or SOLFA-850 for non-ionic surfactant (NIS), and 3-(Dodecyldimethylammonio) propanesulfanate (DDAP) for zwitterionic surfactant (ZIS) was supplemented by 0.05% and 0.1% in Dehority's artificial medium containing Holtein rumen fluid, respectively, and the substrate for fermentation was rice straw passed through 1 mm screen. The experiment was composed of 7 treatments (two levels of two NISs, two levels of a ZIS) including the control, and 6, 12, 24, 48 and 72 hr of fermentation time with 3 replications per treatment. Treatment of Tween 80 increased in vitro DM digestibilities during 48 hr and 72 h post fermentations compared to the other treatments, whereas treatment of DDAP as a ZIS resulted in decreased DM digestibility than that of the control from 24 hr post fermentation (P<0.05). Gas production in vitro was greater (P<0.05) with addition of NIS than the control or ZIS, and increased as fermentation time elapsed. Rumen mixed microbial growth was greatest with addition of Tween 80 as NIS, and lowest when DDAP as ZIS was supplemented to the fermentation tube (P<0.05). In SEM observation, rumen microbial population attached on rice straw particle was greater with addition of NIS, but was less with addition ZIS compared with the control. In conclusion we could not found any positive effects of ZIS surfactants on rumunal fermentation characteristics and rumen microbial growth rates.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.583-593
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• 2015

This study was conducted to investigate the effects of mixed-sowing of legume forage and applying of cattle manure on the productivity of organic rye to provide dairy farmers with safe organic feeds. Also the present study aimed to evaluate optimal applying level of cattle manure and carrying capacity of Korean native cattle (Hanwoo) per unit area. The amount of crude protein was significantly higher in the mixed-sowing of legume forage (average 760~786 kg/ha) than in the single-sowing of rye (average 353 kg/ha) (p<0.05). The mixed-sowing of forage peas and the single-sowing of rye tended to improve by increasing the level of nitrous fertilization rather than the fertilization treatment. The amount of crude protein on the rye according to the mixed-sowing of legume was significantly higher in the mixture (average 8.29~9.90%) than in the single (average 4.93%) (p<0.05). The comparison by the level of nitrogen fertilization indicated significantly high for the rye in 50 kg N/ha than in fertilization treatments (p<0.05). Total digestible nutrients (TDN) amount in the single-sowing of rye was average 46.86% and has lower than average 49.96~50.12% of the mixed-sowing of legume, and especially the mixed-sowing of forage pea was the highest with 54.55% in 150 kg N/ha for the level of nitrogen fertilization (p<0.05). The breeding ability of annual organic livestocks per unit area according to the feed value of rye presented significantly higher ability in the mixed-sowing of legume (3.72~4.12 heads) than the single-sowing of rye (average 2.26 heads) (p<0.05). By summarizing above results, the mixed-sowing of legume is required to improve the productivity and the feed value of rye for increasing organic livestock breeding ability in southern area of Gyeongbuk regions, and the study for identifying the appropriate fertilization level using livestock excretions is further necessary.

• Korean Journal of Organic Agriculture
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• v.18 no.2
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• pp.271-282
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• 2010

This study was conducted to investigate the effects of concentrated pig slurry and byproduct liquid fertilizer on the growth and yield of chinese cabbage. The experiment was conducted in a rain-shelter house which was installed in the agriculture farm. Plants were fertilized with concentrated slurry (CS), byproduct fertilizer (BF), mixture of concentrated slurry and byproduct liquid fertilizer (CS+BF), combined organic and chemical fertilizer (CS+BF+BF) and chemical fertilizer (CF) as control. 1. The pH level of byproduct liquid was decreased from the 3rd to the 7th day and increased 9 day to 14th day, but pH of concentrated slurry (CS) was not greatly varied. EC of concentrated slurry (CS) and byproduct liquid was increased gradually during the fermentation. 2. The concentrated slurry (CS) was low in phosphorus, calcium, magnesium, rich in potassium and unbalanced as a low nitrogen and high potassium. But byproduct liquid fertilizer was balanced in nitrogen and potassium ratio. 3. The leaf number, head height, head width of chinese cabbage in treatment with organic and chemical fertilizer (CS+BF+N) showed significant difference compared with control. The plant and head weight of chinese cabbage in treatment of concentrated slurry was severely decreased, but that in treatment organic and chemical fertilizer (CS+BF+N) were increased 8, 10% compared with control chemical fertilizer (CF), respectively. 4. The content of $K_2O$ in plant tissue and in soil was increased after using concentrated slurry. On the other hand, mineral content of except $K_2O$ did not differ significantly between any of the treatments. In conclusion, organic and chemical fertilizer (CS+BF+N) could improve growth and head weight of chinese cabbage.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.667-682
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• 2014

The effects of liquid pig manure (LM) on the yield and quality of rice as well as soil chemical properties were determined in the field of sandy loam soil under the different fertilizer management. Treatments consisted of 100%, 130% and 160% N application rates of liquid manure as calculated on the basis of the recommended rate of nitrogen (9 kg N/10a) for rice cultivation. Chemical fertilizer (CF) was used as control. Concentrations of T-N and T-P in paddy water were measured by 5-day intervals up to 20days after application. LM treatments significantly increased T-N concentrations in paddy water proportionally with increasing rates of LM (13.2 to 25.7 mg/L). Similarly Total-P content in paddy water was increased right after LM applications but was well below the quality standard of wastewater and manure. Plant height and tillers in 100% and 130% N LM treatments were lower than those in CF control. In the 160% LM treatment, however, plant height and numbers of tillers were higher than those in the CF control. Yields in 100% LM and 160% LM plots were decreased by 3 and 5%, respectively, as compared with 422 kg per 10a in the CF plot. Rice protein contents were similar between 100% LM and CF control (about 6.8%) but it was increased to 7.2% and 7.7% in 130% LM and 160% LM treatments, respectively. Toyo-taste value in the 100% LM treatment was higher than in CF control plot. The proportions of perfect grain of the brown rice were lower in 130% LM and 160% LM treatments than that in CF control. Soil organic matter content, heavy metal and exchangeable cations were highest in the 160% LM plot. Thus considering yield and quality of rice and heavy metals contents in soil, 130% N basal application of liquid manure can be recommended for rice cultivation in this experiment.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.531-537
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• 2013

The average annual and winter ambient air temperatures in Korea have risen by 0.7 and $1.4^{\circ}C$, respectively, during the last 30 years. Radish (Raphanus sativus), one of the most important cool season crops, may well be used as a model to study the influence of climatic change on plant growth, because it is more adversely affected by elevated temperatures than warm season crops. This study examined the influence of transplanting time, nitrogen fertilizer level, and climate parameters, including air temperature and growing degree days (GDD), on the performance of a radish cultivar 'Mansahyungtong' to estimate crop growth during the spring growing season. The radish seeds were sown from April 24 to May 22, 2012, at internals of 14 days and cultivated with 3 levels of nitrogen fertilization. The data from plants sown on April 24 and May 8, 2012 were used for the prediction of plant growth as affected by planting date and nitrogen fertilization for spring production. In our study, plant fresh weight was higher when the radish seeds were sown on $24^{th}$ of April than on $8^{th}$ and $22^{nd}$ of May. The growth model was described as a logarithmic function using GDD according to the nitrogen fertilization levels: for 0.5N, root dry matter = 84.66/(1+exp (-(GDD - 790.7)/122.3)) ($r^2$ = 0.92), for 1.0N, root dry matter = 100.6/(1 + exp (-(GDD - 824.8)/112.8)) ($r^2$ = 0.92), and for 2.0N, root dry matter = 117.7/(1+exp (-(GDD - 877.7)/148.5)) ($r^2$ = 0.94). Although the model slightly tended to overestimate the dry mass per plant, the estimated and observed root dry matter and top dry matter data showed a reasonable good fit with 1.12 ($R^2$ = 0.979) and 1.05 ($R^2$ = 0.991), respectively. Results of this study suggest that the GDD values can be used as a good indicator in predicting the root growth of radish.

• Journal of Animal Environmental Science
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• v.12 no.3
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• pp.123-132
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• 2006

This study was conducted to determine the volume of Holstein heifers manure excreted and its characteristics. The average dry matter intake of heifers was 6.7 kg/head/day. The intake rate was lowest in spring among four seasons. The average dry matter intake rate during spring, summer, fall, and winter was 4.6, 8.3, 7.1, and 6.8 kg/head/day, respectively. The average water intake of heifers was $19.3{\ell}/head/day$. The wale. consumption was highest value ($21.8{\ell}/head/day$) in summer and lowest values ($18.3{\ell}/head/day$) in spring and winter. Values were found not to be statiscally different for the four seasons. The average manure production of heifers (average live weight was 363.1 kg) was 20.3 kg/head/day and it was 5.6% of live animal weight. The manure production during spring, summer, fall, and winter was 13.7, 23.5, 25.0, and 20.2 kg/head/day, respectively. Production during spring was lower than the other seasons (p<0.05). A higher correlation between live weight and manure production ($R^2=0.7816$) and between live weight and feed intake ($R^2=0.7296$) was observed for heifers. Correlations between manure production and water intake and between manure production and feed intake were found to be relatively low for heifers. The moisture content of feces was 83.5% and that of urine 94.6%. The pH of feces and urine were in the ranges of 7.4 and 7.5, respectively. The $BOD_5$, COD, SS, T-N, T-P concentrations of the heifer feces were 18,048, 50,114, 119,833, 2,519, and $427mg/{\ell}$, respectively. Heifer urine showed lower levels of $BOD_5(5,434mg/{\ell})$, COD$(6,550mg/{\ell})$, SS$(825mg/{\ell})$, T-N$(3,616mg/{\ell})$, and $T-P(28mg/{\ell})$ than feces. The fertilizer nutrient concentrations of heifer feces was 0.25% N, 0.1% $P_2O_5$ and 0.14% $K_2O$. Urine was found to contain 0.36% N, 0.006% of $P_2O_5$ and 0.31% $K_2O$.

• Journal of Animal Environmental Science
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• v.18 no.sup
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• pp.21-28
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• 2012

Since the temperature during summer is higher than the temperature which is suitable for milking cows, high temperature stress have severely affected dairy farmers due to decreased milk production. This study was carried out to investigate the effect of rainwater sprinkling system for reducing heat stress in milking cows during intense heat time. Treatment conditions were divided into 2 types ; No rainwater sprinkling (T1) and Rainwater sprinkling (T2). Temperature in the milking cow shed, feed intake, milk yield, respiration rate and rectal temperature were measured to investigate the effect of hot environment on the physiological, productive responses in milkig cows. The results obtained are summarized as follows : The daily milk yields were 24.5 and 27.4 kg per head in T1 and T2 during experimental period. The respiration rate was significantly higher in T1 than those of T2. The rectal temperature in T1 ($40.6^{\circ}C$) was higher than those of T2 ($39.8^{\circ}C$). It was suggested that rainwater sprinkling system installed in the dairy cow shed was effective means to reduce heat stress of milking cow.