• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.21 no.6
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• pp.1-20
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• 2022

The basic way to charge vehicles for using road and public transport networks is the entry-exit toll system. This system works by reading Hi-Pass and public transportation cards of the vehicles using card readers. However, the problems of navigating a route in consideration of entry-exit toll systems include the non-additive costs of enumerating routes. This problem is known as an NP-complete task that enumerates all paths and derives the optimal path. So far, the solution to the entry-exit toll system charging has been proposed in the form of transforming the road network. However, unlike in the public transport network where the cards are generalized, this solution has not been found in situations where network expansion is required with a transfer, multi-modes and multiple card readers. Hence, this study introduced the Link Label for a public transportation network composed of card readers in which network expansion is bypassed in selecting the optimal path by enumerating the paths through a one-to-one k-path search. Since the method proposed in this study constructs a relatively small set of paths, finding the optimal path is not burdensome in terms of computing power. In addition, the ease of comparison of sensitivity between paths indicates the possibility of using this method as a generalized means of deriving an optimal path.

• Korean Journal of Animal Reproduction
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• v.12 no.1
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• pp.51-62
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• 1988

Spleen cells of mouse immunized with hCG were fused with myeloma cell (SP 2/0 Ag 14) to produce monoclonal antibody against hCG. Several clones of hybridoma secreting monoclonal antibody were established and antibodies were characterized in terms of titer, subisotyping and sensitivity in immunoassay. Several methods, for the purification of anti¬bodies, based on gel-filtration, DEAE-ion exchange chromatography and affinity chromatography. were applied and compared each other by the result of SDS-PAGE. Two-site immunochemiluminometric assay (ICMA) involving the use of an excess concentration of a specific monoclonal antibody passively adsorbed onto the walls of plastic tubes and a chemiluminescence labelled antibody conjugate were de¬veloped for the determination of hCG as a preliminary study.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.48 no.4
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• pp.102-107
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• 2011

In this paper, a microfluidic array biochip for simultaneously detecting multiple antigen-antibody bindings was designed and implemented. The biochip has the single channel in which microreaction chambers are serially connected, and the antibody-coated microbeads are packed in each microreaction chamber. In addition, the weir structure was fabricated in the microchannel using the gray-scale photolithography in order to trap the microbeads in the microreaction chamber. Three kinds of antibodies were chosen, and the antibodies were immobilized onto the microbeads by the streptavidin-biotin conjugation. In the experiment, as the fluorescence-labeled antigens were injected into the microchannel, the antigen-antibody bindings were completed in 10 minutes. When the solution with multiple antigens was injected into the microchannel, it was observed that the fluorescence intensity increased in only the corresponding microreaction chambers with few non-specific binding. The microfluidic array biochip implemented in this study provides, even with the consumption of tiny amount of sample and fast reaction time to simultaneously detect multiple immunoreactions.

• Journal of Korean Society of Transportation
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• v.22 no.6
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• pp.101-108
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• 2004

The new transit fare in the Seoul Metropolitan is basically determined based on the distance-based fare system (DBFS). The total fare in DBFS consists of three parts- (1) basic fare, (2) transfer fare, and (3) extra fare. The fixed amount of basic fare for each mode is charged when a passenger gets on a mode, and it proceeds until traveling within basic travel distance. The transfer fare may be added when a passenger switches from the present mode to another. The extra fare is imposed if the total travel distance exceeds the basic travel distance, and after that, the longer distance the more extra fare based on the extra-fare-charging rule. This study proposes an algorithm for finding minimum fare route in DBFS. This study first exploits the link-label-based searching method to enable shortest path algorithms to implement without network expansion at junction nodes in inter-modal transit networks. Moreover, the link-expansion technique is adopted in order for each mode's travel to be treated like duplicated links, which have the same start and end nodes, but different link features. In this study, therefore, some notations associated with modes can be saved, thus the existing link-based shortest path algorithm is applicable without any loss of generality. For fare calculation as next steps, a mathematical formula is proposed to embrace fare-charging process using search process of two adjacent links illustrated from the origin. A shortest path algorithm for finding a minimum fare route is derived by converting the formula as a recursive form. The implementation process of the algorithm is evaluated through a simple network test.

• Tuberculosis and Respiratory Diseases
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• v.41 no.5
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• pp.504-512
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• 1994

Background: It is well known that severe hypoxemia is often associated with liver cirrhosis without preexisting cardiac or pulmonary diseases. Pulmonary vascular impairments, more specifically, intrapulmonary shunting have been considered as a major mechanism. Intrapulmonary shunting arises from pulmonary vascular dilatation at the precapillary level or direct arteriovenous communication and has relationship with the characteristic skin findings of spider angioma. However, these results are mainly from Western countries where alcoholic and primary biliary cirrhosis are dominant cuases of cirrhosis. It is uncertain that the same is true in viral hepatitiss associated liver cirrhosis, which is dominant causes of liver cirrhosis in Korea. We investigated the incidences of hypoxemia and orthodeoxia in Korean cirrhotic patients dominantly composed of postnecrotic cirrhosis and the significance of intrapulmonary shunting as the suggested mechanism of hypoxemia, Method: We performed the arterial blood gas analysis separately both at the supine and errect position in 48 stable cirrhotic patients without the evidences of severe complications such as ascites, variceal bleeding, and hepatic coma. According to the results of arterial blood gas analysis, all patients were divided into hypoxemic and normoxemic group. In each group, pulmonary function test and Tc-99m-MAA whole body scan were performed. The shunting fraction was calculated based on the fact that the sum of cerebral and bilateral renal blood flow is 32% of the systemic blood flow. Results: The hypoxemia of $PaO_2$ less than 80 mmHg was observed in 9 patients(18.8%) and Orthodeoxia more than 10 mmHg was observed in 8 patients(16.7%). But there was no patient with significant hypoxemia of $PaO_2$ less than 60 mmHg. $PaO_2$ was significantly decreased in the patients with spider angioma than the pathients without spider angioma and showed no correlation with the serologic type and severities of liver function test findings. Any parameters of pulmonary function test did not demonstrate the difference between normoxemic and hypoxemic group. But hypoxemic group showed significantly increased shunt fraction of $11.4{\pm}4.1%$ than normoxemic group of $4.1{\pm}2.0%$ (p<0.05). Conclusions: Hypoxemia is not infrequently observed complication in liver cirrhosis and intrapulmonary shunting is suggested to p1ay a major ro1e in the development of hypxemia. But there was no great likelihood of clinically significant hypoxemia in our domestic cirrhotic patients predominantly composed of postnecrotic type.