• Title/Summary/Keyword: 보리 유식물

Search Result 13, Processing Time 0.028 seconds

Effects of Dimethipin on the Photosynthetic Electron Transport Activity of Isolated Barley Chloroplasts (보리 유식물 분리엽록체의 광합성 전자전달활성에 미치는 Dimethipin의 영향)

  • Lee Joon Sang
    • Korean Journal of Environmental Biology
    • /
    • v.23 no.1
    • /
    • pp.52-56
    • /
    • 2005
  • Eight days grown barley seedlings were treated with dimethipin for 72 hours and then the content of chlorophyll and photosynthetic electron activities of isolated chloroplasts were investigated. At the treatment of 10/sup -5/ M dimethipin the content of chlorophyll was decreased to 33% at 72 hours. Seven days etiolated barley seedlings were exposed to the light while dimethipin was added. At the time of 48 hours' greening chlorophyll content was reduced to 43% at 10/sup -4/M dimethipin and the chlorophyll a/b ratio was increased. In photosynthetic electron transport the activity of PSⅡ+PSⅠ was decreased to 10% at 48 hours and 25% at 72 hours at 10/sup -4/ M dimethipin. In the treatment of 10/sup -4/ M dimethipin the activity of PSⅡ+PSⅠ, except water splitting system was inhibited to 16% at 48 hours and 27% at 72 hours. The activity of PSⅡ was inhibited to 8% at 24 hours, 13% at 48 hours and 18% at 72 hours at 10/sup -4/ M dimethipin. The activity of PSⅠ was inhibited to 4% at 24 hours, 8% at 48 hours and 10% at 72 hours at 10/sup -4/ M dimethipin. In the times of greening of 7 days etiolated barley seedlings the activities of PSⅡ+PSⅠ were reduced to 5, 10, 10 and 11 % at 6, 12, 24, and 48 hours, respectively, at 10/sup -4/ M dimethipin. On the other hand, the activity of PSⅡ+PSⅠ except water splitting system, was not inhibited at all incubated hours in 10/sup -4/M dimethipin and there were no clear changes of the activities of PSⅡ and PSⅠ as compared to the control. Therefore, it could be concluded that dimethipin inhibited the photosynthetic electron activity by affecting the function of chloroplast rather than the synthesis of chloroplast and the inhibited function of chloroplast seems to come from the severe decrease of chlorophyll content.

Effects of Ozone on Photosynithetic Activity in Chloroplast of Barley (Hordeum vulgare L.) (오존이 보리(Hordeum vulgare L.) 엽록체의 광합성 활성에 미치는 영향)

  • 정화숙
    • Journal of Plant Biology
    • /
    • v.38 no.4
    • /
    • pp.399-407
    • /
    • 1995
  • The effects of 0.5 and 1.0 ppm of ozone on the developing chloroplast of barley (Hordeum vulgare L) seedling during greening were investigated by PSI and II activities, chlorophyll fluorescence, and the contents of chlorophyll. Etiolated barley seedling was treated 0.5 and 1.0 ppm ozone for the first 4 h during greening. In 24 h greening experiment, the contents of chlorophyll were decreased by increasing ozone concentration from 0.5 ppm to 1.0 ppm. In 24 h greening experiment, Fo, Fv, Fm and qE were greatly decreased as the concentration of ozone was increased, but those were not considerably decreased in 48 h greening experiment. In another experiment, the developing barley seedling was treated with 0.5 and 1.0 ppm ozone for the last 4 h during greening period, which was 24 h or 48 h. In both experiments the PS II activity was decreased as the concentration of ozone was increased, but not in PS I activity. Fv, Fm and Fv/Fo were also decreased as the concentration of ozone was increased. qP and qR were strikingly decreased as the concentration of ozone was increased in both experiments.iments.

  • PDF

Changes of Sucrose Content and Invertase Activity in Leaves of Barley Seedlings under Low Temperature (저온 처리한 보리 유식물 잎의 설탕함량과 Invertase의 활성변화)

  • 이명애
    • Journal of Plant Biology
    • /
    • v.35 no.1
    • /
    • pp.91-95
    • /
    • 1992
  • Changes of contents of reducing sugar and sucrose and activities of sucrose-phosphate synthase, sucrose synthease and invertase from the leaves of barley (Hordeum vulgare L. cv. Chalssal) seedlings grown at $4^{\circ}C$ were investigated, and the property of acid invertase were also examined. In the seedlings grown at $4^{\circ}C$ for 3 days, the contents of reducing sugar and sucrose were increased to 1.3 and 2.4 times, respectively. Activity of acid invertase was decreased markedly by cold treatment while the activities of sucrosephosphate synthase, sucrose synthase, and alkaline invertase were not changed. In acid phosphatase purified partially by ammonium sulfate fractionation and DEAE-Sephacel column chromatography, the $K_m$ value for sucrose was 9.5 mM and the optimum pH and temperature was 5.5 and $35^{\circ}C$ respectively. This enzyme was supposed to be ${\beta}-fructosidase$ by studies on the substrate specificity and the molecular weight was estimated to be 63 Kd by Sephadex G-200 gel chromatography.graphy.

  • PDF

Changes of Phosphorylcholine Metabolism in Barley Seedlings during Greening (녹화중 보리유식물에서 Phosphorylcholine대사의 변화)

  • 유경희
    • Journal of Plant Biology
    • /
    • v.31 no.4
    • /
    • pp.259-266
    • /
    • 1988
  • We investigated the activities of choline kinase, CTP: phosphorylcholine cytidyltransferase, and phosphatase during the greening of etiolated barley seedlings. Activities of choline kinase in leaves increased until 6 hours after illumination and decreased considerably after 6 hours, while activities of CTP: phosphorylcholine cytidyltransferase increased after illumination. On the contrary, changes of these two enzymatic activities showed reverse pattern in roots. The activities of phosphatase which hydrolyze phosphorylcholine decreased in leaves but changed little in roots during greening. The concentration of phosphorylcholine increased in xylem exudate and in roots during greening, while decreased in leaves. These results suggested that more phosphorylcholine arrive in leaves from roots as greening of etiolated barley seedlings.

  • PDF

The Effects of ozone on the greening of barley (Hordeum vulgare L.) seedling (보리 (Hordeum vulgare L.) 유식물의 녹화에 미치는 오존의 영향)

  • 박강은;정화숙
    • Journal of Environmental Science International
    • /
    • v.5 no.4
    • /
    • pp.545-553
    • /
    • 1996
  • The effects of 0.2 ppm ozone on the developing chloroplast of barley (Hordeum vulgare L.) seedling during greening were examined by chlorophyll contents, photosynthetic electron tiansport activity, Fo, Fv and fluorescence quenching coefficient. Chlorophyll contents of seedling treated with ozone were not changed in comparison with the control during the 96 h greening experiment, but PS II activity of the chloroplasts of seedlings treated with ozone was decreases by- l5%. Fo was slightly decreased but Fv was decreases by 5% in comparison with the control, In fluorescence quenching analysis, qP and qE were decreased by 11% and 9%, respectively, in comparison with the control. These results suggest that oxidation site of PS II is the site affected mostly and PQ pool is also affected slightly by 0.2 ppm ozone.

  • PDF

Changes of Chloroplast-Mediated Electron Transport Activity and Chlorophyll-Protein Complexes in Barley Seedlings by Decursinol (Decursinol 처리에 따른 보리 유식물의 전자전달 활성과 엽록소-단백질 복합체의 변화에 대하여)

  • 이현식
    • Journal of Plant Biology
    • /
    • v.31 no.2
    • /
    • pp.131-141
    • /
    • 1988
  • The effects of decursinol and decursin on chloroplast-mediated electron transport and phosphorylation in barley seedlings were investigated in comparison with coumarin in the dark or light. The changes of CP-complexes were also studied. Decursinol, decursin and coumarin caused marked inhibitory effects on germination of seed and electron transport and phosphorylation activity of seedlings. The following order of inhibitory effectiveness was exhibited; decursinol>coumarin>decursin. Loss of chlorophyll and decrease of electron transport activity were retarded in the dark, but were reversely accelerated in the light by these three chemicals. The changes of CP-complex patterns were also similar to effects on chlorophyll content and the electron transport activity. These opposite effect in the dark and light suggest that these three chemicals act as natural growth retardants rather than cytokinins or growth inhibitors.

  • PDF

The effects of aqueous extracts of plant roots on germination of seeds and growth of seedings (식물근의 추출물질이 종자발아 및 유식물의 생장에 미치는 영향)

  • Chan-Ho Park
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.4 no.1
    • /
    • pp.1-23
    • /
    • 1968
  • This study aimed at contributing to the improvement of cropping systems after finding out the effects of excrements and components of crop root influence on other crops as well as themselves. The following forage crops suitable for our country were selected for the present study. Aqueous extracts of fresh roots, aqueous extracts of rotting roots and aqueous solutions of excrements of red clover, orchard grass and brome grass were studied for the effects influencing the germination and growth of seedlings of red clover, ladino clover, lespedeza, soybean, orchard grass, Italian ryegrass, brome grass, barley, wheat, sorghum, corn and Hog-millet. In view of the possibility that the organic acid might be closely related to the excrements and components of crop root connected with soil sickness, the acid components of three species of roots were analysed by paper chromatography and gas chromatography method. The following results were obtained: 1. Effects of Aqueous Extracts of Fresh Roots : Aqueous extracts of red clover: The extracts inhibited the growth of seedlings of the ladino clover and lespedeza and also inhibited the development of most crops except that of sorghum among the Graminaceae. Aqueous extracts of orchard grass: The extracts promoted the seedlings growth of red clover and soybean, while it inhibited the germination and growth of orchard grass. There were no noticeable effects influencing other crops while it inhibited the growth of barley and Hog-millet. Aqueous extracts of brome grass: There was no effect on Italian ryegrass but there was an inhibiting effect on the other crops. 2. Effects of Aqueous Extracts of Rotting Roots : Aqueous extracts of red clover: The extracts promoted the seedling growth of red clover. But it reflected the inhibiting effects on other crops except sorghum. Aqueous extracts of orchard grass: The extracts promoted the growth of red clover, ladino clover, soybean and sorghun, while it inhibited the germination and rooting of barley and Hog-millet. Aqueous extracts of brome grass: The extracts gave the promotive effects to the growth of red clover, soybean and sorghum, but caused inhibiting effects on orchard grass, brome grass, barley and Hog-millet. 3. Effects of Aqueous Solutions of Excrements : The aqueous solution of excrements of red clover reflected the inhibition effects to the growth of Graminaceae, while the aqueous solutions of excrements of orchard grass and Italian ryegrass caused the promotive effects on the growth of red clover. 4. Results of Organic Acid Analysis : The oxalic acid, citric acid, tartaric acid, malonic acid, malic acid and succinic acid were included in the roots of red clover as unvolatile organic acid, and in the orchard grass and brome grass there were included the oxalic acid, citric acid, tartaric acid and malic acid. And formic acid was confirmed in the red clover, orchard grass and brome grass as volatile organic acid. In consideration of the results mentioned in above the effects of excrements and components of roots found in this studies may be summarized as follows. 1) The red clover generally gave a disadvantageous effect on the Graminaceae. Such trend was considered chiefly caused by the presence of many organic acids, namely oxalic, citric, tartaric, malonic, malic, succinic and formic acid. 2) The orchard grass generally gave an advantageous effect on the Leguminosae. This may be due to a few kinds of organic acid contained in the root, namely oxalic, citric, tartaric, malic and formic acid. Furthermore a certain of promotive materials for growth was noted. 3) As long as the root of brome grass are not rotten, it gave a disadvantageous effect on the Leguminosae and Graminaceae. This may be due to the fact that several unidentified volatile organic acid were also included besides the confirmed organic acid, namely oxalic, citric, tartaric, malic and formic acid. 5. Effects of Components in Roots to the Soil Sickness : 1) It was considered that the cause of alleged red clover's soil sickness did not result from the toxic components of the roots. 2) It was recognized that the toxic components of roots might be the cause of soil sickness in case the orchard grass and brome grass were put into the long-term single cropping. 6. Effects of Rooted Components to the Companion Crops in the Cropping System : a) In case of aqueous extracts of fresh roots and aqueous excrements (Inter cropping and mixed cropping) : 1) Advantageous combinations : Orchard grass->Red clover, Soybean, Italian ryegrass->Red clover, 2) Disadvantageous combinations : Red clover->Ladino clover, Lespedeza, Orchard grass, Italian ryegrass, Fescue Ky-31, Brome grass, Barley, Wheat, Corn and Hog.millet, Orchard grass->Lespedeza, Orchard grass, Barley and Hog-millet, Brome grass->Red clover, Ladino clover, Lespedeza, Soybean, Orchard grass, Brome grass, Barley, Wheat, Sorghum, Corn and Hog-millet, 3) Harmless combinations : Red clover->Red clover, Soybean and Sorghum, Orchard grass->Ladino clover, Italian ryegrass, Brome grass, Wheat, Sorghum and Corn, Brome grass->Italian ryegrass, b) In case of aquecus extracts of rotting roots(After cropping) : 1) Advantageous combinations : Red clover->Red clover and Sorghum, Orchard grass->Red clover, Ladino clover, Soybean, Sorghum, and Corn, Brome grass->Red clover, Soybean and Sorghum, 2) Disadvantageous combinations : Red clover->Lespedeza, Orchard grass, Italian ryegrass, Brome grass, Barley, Wheat, and Hog-millet Orchard grass->Barley and Hog-millet, Brome grass->Orchard grass, Brome grass, Barley and Hog-millet, 3) Harmless combinations : Red clover->Ladino clover, Soybean and Corn, Orchard grass->Lespedeza, Orchard grass, Italian ryegrass, Brome grass and Wheat Brome gass->Ladino clover, Lespedeza, Italian ryegrass and Wheat.

  • PDF