• Journal of agriculture & life science
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• v.44 no.1
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• pp.9-15
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• 2010

Barley, a monocotyledonous plant, is relatively recalcitrant to the process of Agrobacterium-mediated genetic transformation. In this study, seedlings of six barley cultivars (Keunal-1-Ho, Saessal, Ol, Saechalssal, Seodunchal and Pungsanchalssal) were injured using alkali, oxidizing or reducing agents. They were then transformed using Agrobacterium via vacuum infiltration for the analysis of comparative GUS gene expression. It was determined that chemical injuries causing a slight growth retardation could overall enhance the GUS transformation rate. Hydrogen peroxide was determined to be the most effective.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.161-165
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• 1997

This experiment were carried out to clarify the effects of several factory wastes on the emergence and seedling growth of five crop species, rice, Chinese cabbage, melon, and tomato. Wastes of three factories treated by several concentrations on the soil in which crop were seeded. In rice seedling experiments, the rice seedlings were treated with factory wastes hydroponically. Factory wastes used in the experiment were obtained from leather, phenol resin, and dye factory. The growth of rice seedlings was inhibited by each factory wastes, but the dry weight of rice seedling was increased by the low concentration below 1/16 dilution of leather factory waste. During 15 days, dry matter accumulation of rice seedlings treated with undiluted factory wastes decreased to 46.0, 51.4, -5.4% of control by treating wastes of phenol resin, leather, and dye factory respectively. The injury of crops by leather factory waste was severe in tomato but slight in barley. Waste of phenol resin factory affects highly both on Chinese cabbage and on melon. When dye factory waste was treated on each crop, all plants died in the treatments of waste solution which diluted to 1/8 of original waste. Tomato and melon were most sensitive crop species to the waste of dye factory. Although the responses of crops to each factory waste were various, the degree of injuries were more higher in vegetables than cereal crops.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.562-568
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• 1995

Changes in rice yield and soil chemistry of dry seeded rice in double cropping of rice and barley were investigated at National Yeongnam Agricultural Experiment Station during 5 years (1990-1994). Barley straw added or removed dry seeding had safe rice seedling stand for good rice production. However, the coefficient of variation of seedling stand by year was higher than dry seeding in rice single crop. Rice seedling height was slightly reduced by barley straw incorporation. However, it recovered soon as rice growing progressed. Heading time delayed 4 days in flat seeding method but not in high-ridged seeding method which imply that. this is more safe seeding technology than flat seeding method. Comparing to rice single crop, barley straw added dry seeding increased rice yield by 2∼19%(Average of 5 years: 8%) from second year due to higher panicle and spikelet number. Contents of organic matter was 2.71% in 1990. In 1994, it decreased 2.61% for rice single crop, while increased 3.02% and 3.41% for barley straw removed or added field, respectively. Rice grain quality was not significantly different among cropping patterns.

• Korean Journal of Organic Agriculture
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• v.15 no.4
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• pp.413-424
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• 2007

This trial was carried out to determine effects of TMR(Total Mixed Ration) in comparison to conventional diet on feed intake, digestibility, and nitrogen retention in Korean black goats. Twelve Korean black goats were allotted to treatments in four groups of three goats and the they were housed in separate metabolism cages for 21 days. Treatments included conventional diet(A: rice straw and commercial concentrates) as a control group and three TMR groups(B: rice straw and commercial TMR; C: 30% organic whole barley silage and rice straw with organic grain; D: 60% organic whole barley silage with organic grain). The results obtained are summarized as follows. Dry matter intake, fecal excreta, digestible dry matter, metabolic intake and feed intake of BW were significantly higher(p<0.05) compared with A, B, C treatment. The digestibility for most of nutrients except NFC was significantly higher(p<0.05) for D treatment than the others. On the other hand, the digestibility of NFC was higher for B treatment than for the others, even if it was not significantly different across treatments. Nitrogen intake was lower for D treatment, but the significant differences were not found across treatments. N retention rate was higher for B treatment with significant difference. For the blood composition, HDL cholesterol and Immunoglobulin G were significantly higher(p<0.05) for C, D treatment than the others. The results showed that, in comparison with A treatment(conventional diet) and B treatment(commercial TMR), dry matter intake, nutrient digestibility and N retention rate were not lower in C treatment(30% organic whole barley silage) and HDL cholesterol was increased after experiment. Therefore, it could be concluded that TMR with organic whole barley silage might contribute to the production of a high quality and safer meat in goat production.

• Journal of Conservation Science
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• v.26 no.1
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• pp.95-107
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• 2010

Biochemical research was carried out on 4 human skeletal remains from historical lime-layered tombs assigned to the Joseon Dynasty in Oknam-ri, Seocheon. The preservation of femur was evaluated by stereoscopic microscopy and scanning electron microscopy. Most of specimens showed good histological preservation. The histological results proved to be a good potentiality for biochemical analysis using bio-molecules. The amelogenin gene and mitochondrial DNA (mtDNA) analyses revealed that three specimens perhaps have maternal consanguinity due to sharing with mtDNA haplogroup D4b1, and two specimens buried in the same tomb were a couple in Gatjaegol site. Carbon and nitrogen stable isotope analysis indicated that four deads diet were built around C3 plant as rice, barley, wheat and bean. In this study we characterized genetic and diet features from the social stratum who could make lime-layered tombs during period of the Joseon Dynasty. The results suggest that biochemical research using the human skeletal remains from the Joseon Dynasty has the great potential and reasonable value for archaeology, anthropology, and population genetics.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.229-237
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• 1987

In order to study the biological control of soil-borne disease of sesame, antagonistic isolates of Trichoderma , Bacillus sand streptomyces to Fusarium oxysporum and Rhizoctonia solani were isolated from the rhizosphere soils of sesame plants and some other habitats. Out of the isolates of microorganisms collected a strain of Trichoderma viride was selected as a biological control agent for the study and its effect on the control of damping-off and the seedling growth of sesame was investigated. The results obtained are as follows: 26 percents of Bacillus spp. isolated from the rhizosphere soil of sesame plants showed antagonism to two pathogenic fungi. Important species were B. Subtilis and B. polymyxa. Streptomyces species isolated from the rhizosphere soils of sesame lysed the cell wall of hyphae and conidia of F. oxysporum and reduced conspicuously the formation of macroconidia and chlamydospores of the fungus. 84 percents of Trichoderma spp. isolated from the rhizosphere soil of sesame plants were antagonistic to F. oxysporum and 60 percents of the isolates were antagonistic to both F. oxysporum and R. solani. Trichoderma viride TV-192 selected from antagonistic isolates of Trichoderma spp. was highly antagonistic to F. oxysporum and soil treatment with the isolate reduced notably damping-off of sesame. T. viride TV-192 showed better growth in crushed rice straw, barley straw and sawdust media than F. oxysporum. Sawdust was selective for the growth of T. viride. Supplementation of wheat bran and mixtures of wheat bran and sawdust inoculated with T. viride TV-192 in the soil reduced remarkably damping-off of sesame by F. oxysporum but high density of the fungus TV-192 caused the inhibition of seed germination and seedling growth of sesame. Inhibitory effects of Trichoderma species on seed germination and seedling growth of sesame were different according to the isolates of the fungus. Normal sesame seedlings on the bed treated with the fungus showed better growth than not treated seedlings.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.152-159
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• 1997

Six pathogenic Pythium isolates obtained from diseased ginger rhizomes were identified as Pythum myriotyrum Drechsler based on various morphological and physiological characteristics. The isolates showed strong virulence on underground parts of buds, crowns, rhizomes, roots and aerial parts of leaves and stems as well. The isolates caused rot of germinated seeds of 10 different crops tested, including cucumber and pepper, and markedly inhibited seedling growth of 3 crops tested, including corn and barley. Maximum, optimum and minimum growth temperatures for P. myriotylum were 39~45$^{\circ}C$, 33~37$^{\circ}C$ and 5~7$^{\circ}C$, respectively. Optimum pH for the growth was 6~7. Mycelial linear growth was most rapid on V-8 juice agar, but aerial mycelia were most abundant on PDA and corn meal agar. Zoosporangial and oogonial formation was greatest on V-8 juice agar. Optimum temperatures for the production of zoosporangia and oogonia were 20~35$^{\circ}C$ and 15$^{\circ}C$, respectively.