• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.87-91
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• 2015

There is no specific data on Entomogenous fungus (DongChungHaCho) in accordance with the silkworm varieties, despite of very high value industrial use as functional materials at domestic and abroad. In this study, we investigated culture condition and characteristics of Paecilomyces tenuipes in 3 silkworm cultivars and 2 specific silkworm breeds. Infection rate of P. tenuipes for silkworm varieties was the highest Kumokjam, followed by Baegokjam, Daeseongjam, Golden silk, Yeonnokjam in that order. The optimum culture conditions were as follows: dark condition, $22^{\circ}C{\pm}1$, and about $4cm^2$ in planting density per pupa. Also, we have developed annual production technologies of P. tenuipes. First, in order that the moisture content of the infection pupa became 3% or less in the natural condition. Second, the dried pupa must be vacuum-packed and was kept under $4^{\circ}C$. Finally, by restoring the moisture content of the dried pupa and culture in optimal condition, the technique for annual production of the P. tenuipes was achieved. Therefore, we expect that the use of Kumokjam and annual production technique will contribute significantly to farm income.

• Analytical Science and Technology
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• v.25 no.6
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• pp.483-491
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• 2012

This study is to investigate the issues on how to secure stability during the purification process for the production of recombinant protein A. The final recombinant protein A is produced by passing through the cation exchange column (SP) and the anion-exchange column (Q) during the production process, for which the samples produced by the step-by-step processes can be exposed to trouble in securing stable storage in case the next process cannot be taken within the proper time period. Accordingly, this study aims to evaluate the proper storage conditions and length of time when storing samples produced in the production process. That is, in this study, how to store fair samples, how long the storage period should be set up, and how to evaluate the security of its quality depending on time are dealt with. The items to be experimented with were enodotoxin, SDS-PAGE, HPLC purity and concentration. Experimental results showed that after passing the cation exchange column, when stored at $4^{\circ}C$ or room temperature, SDS-PAGE showed a major band, endotoxin is 5.0 Eu/mg or less, and concentration is on average of 8.21 to 8.24 mg/mL and RSD% 0.10~0.62%. In addition, HLPC purity showed somewhat stable results; at the HPLC purity 214 nm, the average is 99.24% to 99.37% and RSD% is 0.22~0.29%, while the average is 89.72% to 89.80% and RSD% 0.62~1.26% at 280 nm. On the contrary, after passing the anion exchange column, when stored at $4^{\circ}C$ or room temperature, SDS-PAGE revealed the major band, endotoxin is 0.5 Eu/mg or less, and concentration is on average of 5.59 mg/mL and RSD% 0.03~0.10%. when it comes to HLPC purity, the result showed that at the HPLC purity 214 nm, the average is 99.74% and RSD% is 0.10~0.11%, while the average is 96.16% to 96.85% and RSD% 0.72~1.13%. In conclusion, the stability of fair samples of recombinant protein A during the manufacturing process could be obtained without substance decomposition for 7~8 days at $4^{\circ}C$ or 20~21 days at room temperature.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.194.1-194.1
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• 2016

방사성 폐기물의 운반이나 장기 보관 시 방사성 물질의 침출을 차단하기 위한 유리화 기술을 실현하기 위해 이송식 아크 플라즈마에 대해 전산해석을 수행하였다. 본 연구에서는 운전전류나 아크길이와 같은 운전조건 변화에 따른 열플라즈마의 특성 변화 뿐만 아니라 150 kW급 고출력 이송식 아크 플라즈마의 최적 설계를 위하여 핵심 부품인 파일럿 노즐의 길이와 직경 변화에 따른 예상 용융영역을 전산해석 하여 방사성 폐기물의 유리화 기술을 상업적으로 이끌어내는데 기초 자료를 제공하고자 하였다. 노즐직경은 4, 5, 6 mm로 변화시켰으며, 길이는 2, 4, 6mm로 하였다. 이러한 다양한 설계조건에 대하여 운전변수로는 전류 200 A, 방전 기체인 알곤의 유량 15 L/min, 아크 길이 2 cm로 고정하였다. 전산해석 결과 노즐직경이 작을수록 아크압축 효과에 의해 중심부에서 최고 온도가 높은 열플라즈마 제트를 발생시킬 수 있으나, 반경방향으로 온도구배가 커서 고온 구간이 급격히 감소하는 경향이 예상되었다. 반면 노즐직경이 증가할수록 아크 압축효과는 줄어들지만 반경방향으로 온도가 완만히 감소하여 콘크리트가 대부분인 유리화 대상물질을 충분히 용융시킬 수 있는 $2,600^{\circ}C$ 이상의 고온 면적이 넓어지게 될 것으로 예상되었다. 또한, 노즐길이가 줄어들 경우 아크방전의 안정성은 다소 떨어 질 수 있으나 수 있으나 고온의 열플라즈마 제트가 반경방향으로 효과적으로 넓어 질 수 있음이 예측되었다. 따라서 고온 영역의 확장 관점에서 이송식 아크 플라즈마 토치를 제작할 경우 아크의 안정성을 유지하는 범위 내에서 파일럿 노즐의 직경을 크게 하고 길이는 짧게 하는 것이 효과적인 유리화를 위해 유리할 것으로 예상되었다.

• Korean Journal of Microbiology
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• v.21 no.3
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• pp.109-114
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• 1983

Physiological conditions for hydrogen evolution by Rhodopseudomonas sphaeroides K-7 are examined. Larger amount of molecular hydrogen was evolved at $30^{\circ}C$, pH6.8-7.0 under anaerobically illuminated condition of about 12, 000 lux by the organism. The heighest rates of hydrogen evolution were observed in the culture with the organic acids such as acetate, DL-lactate or DL-malate in media containing L-glutamate as a nitrogen source. Hydrogen was also evolved from glucose with the rate of $178.9\;{\mu}l/hr/mg$ cells (dry weight). When glucose was adopted a a sole carbon source, however, conside erable time lag of about 20hours was required for hydrogen evolution. The resting cells stored at $30^{\circ}C$ under argon maintained the rate of hydrogen evolution in nearly about 90% of initial one even 40 days of storation.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2002.10a
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• pp.73-76
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• 2002

The temperature and humidity of dirt-walled storehouse are observed in order to verify the traditional storehouse (main material : soil) during 7days (4th. $Sep{\sim}10th$. Sep). Because the width of the daily variation of temperature is small as $2.5^{\circ}C{\sim}5^{\circ}C$, the reduction of energy supply for low temperature is expected. Because the relative humidity is high and it's variation is small ($4%{\sim}9%$), the inner humidity of dirt-walled storehouse is easily maintained above 80% is expected.

• Journal of the Korea Society for Simulation
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• v.27 no.2
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• pp.25-33
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• 2018

When developing military equipment, it should be designed considering the temperature condition so that the operator can operate in a stable environment. The shelter for storing various military equipments is operated in various environments. The storage temperature and operating requirements of the test equipment and repair accessories shall be $-32^{\circ}C$ to $50^{\circ}C$ and the inside of the shelter shall be designed to meet the storage temperature and operating requirements. In this study, thermal analysis of a 2.5 ton military shelter operating under high temperature and solar heat conditions is performed considering MIL-STD-810G. The thermal analysis was applied by using the concept of heat resistance and heat circuit, and the solar thermal test was performed on the actually manufactured military shelter in order to verify the analysis results.

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.77-83
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• 2006

본 연구는 개 동결 정액 융해 시 straw 크기 및 융해 속도가 융해 정자의 질(quality)에 미치는 영향을 조사하고 최적의 융해 조건을 조사하는데 그 목적이 있다. 정상적인 번식능을 가진 비글 수컷 5마리에서 정액을 채취하여 원심 분리하여 정장을 버리고 남은 정자에 동결보호제인 glycerol이 첨가된 tris-glucose-egg yolk extender를 첨가하여 동결하고 액체질소에 보관한 후 융해하였다. 동결 융해 조건에 따른 효과를 알아보기 위해 straw는 0.25 ml과 0.5 ml크기를 사용하였고 융해 조건은 $75^{\circ}C$에 10초, $55^{\circ}C$에 12초 및 $37^{\circ}C$에서 120초로 하여 융해 후 정자의 활력도(vigor), 운동성(motility), Hypo-osmotic test(HOS test)를 이용한 생존성(viability) 및 $SperMac^{\circledR}$ 염색을 하여 정자의 membrane integrity를 비교 조사하였다. 조사 결과 0.5 ml 크기의 straw를 사용한 경우 $37^{\circ}C$ 융해가 $55^{\circ}C,\;75^{\circ}C$ 융해보다, 0.25 ml 크기의 straw를 사용한 경우에는 $37^{\circ}C,\;55^{\circ}C$ 융해가 $75^{\circ}C$ 융해보다 유의적으로 높은 활력 지수 및 생존성을 보였다(P<0.05). Straw크기에 따라 비교하였을 경우 0.5 ml 군에서 유의적으로 높은 활력도, 생존성 및 membrane integrity를 보였다(P<0.05). 결론적으로 개 정액이 동결 및 융해 시 0.5ml straw를 이용하여 동결한 후 $37^{\circ}C$에서 120초 동안 융해하는 것이 최적의 조건임이 사료된다.

• Food Science and Preservation
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• v.9 no.2
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• pp.126-130
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• 2002

Optimal storage conditions were investigated for 'Fuyu' persimmon fruits and cucumbers were stored under conditions of 70% relative humidity(RH) at 25$^{\circ}C$, 80% RH at l0$^{\circ}C$ and 90% at 5$^{\circ}C$ or l0$^{\circ}C$ of relative humidity, respectively and their qualities in microbial counts, decay ratio, surface color difference and chemical attributes were monitored during the storage period. 'Fuyu'persimmon fruits and cucumbers stored under 90% of relative humidity showed the minimal change in weight loss, decrease of ascorbic acid content, surface color difference and decay by putrefactive microorganisms. As the results of this experiment, 'Fuyu' persimmon fruits and cucumberf stored under 9% of relative humidity were best fer maintaining their freshness.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.11 no.1
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• pp.19-27
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• 1993

For the design of a large-scale landfill, the future utilization plan of the landfill ought to precede based on the analysis of existing facility. Analysis for the present condition of reclamation must include accurate assesment of volume and other consideration such as urban scenery. In this study an optimum data interpolation scheme area/volume determination method based on the classification of topography were combined for the correct assessment of sweeping volume. Combined model was compared with the real data of Digital Elevation Model constructed by aero photography. The new model aims at providing basic information for the design and utilization of a new landfill. A a result of this study, we made an algorithm to perform the classification of the topography in the area of interest objectively. In addition, we decided optimal data interpolation scheme and area/volume calculation method for given topography. Finally, we applied the developed methodology to Nangido Landfill to assess current landfill situation and potential capacity when landfilling is resumed.

• Analytical Science and Technology
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• v.15 no.3
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• pp.229-235
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• 2002

Plasma source ion implantation (PSII) technique was utilized to improve the wettability of polystyrene surfaces. It is well known that treated surfaces undergo aging, leading to hydrophobic recovery with time. We investigated the aging effect of polystyrene thin film on the various molecular weights. Polystyrenes with several molecular weights ($M_w$ = 760, 2430, 31600, 115700, 280000, 903600) were treated in different experimental conditions including gas species and pulse energy, and their hydrophilicity was measured by contact angle goniometer. To study wettability decay as a function of the molecular weight, PSII-treated samples were aged at different temperatures. Hydrophobic recovery of high molecular weight polystyrene was much slower than that of low molecular weight, even at high temperatures. The methods used to characterize treated surfaces were water contact angle measurement, TOF-SIMS, XPS, SEM and AFM.