• 한국가시화정보학회:학술대회논문집
• /
• 2006.12a
• /
• pp.25-35
• /
• 2006

We have evaluated the performances of the following six interpolation schemes used for window deformation in particle image velocimetry (PIV): the linear, quadratic, B-spline, cubic, sinc, Lagrange interpolations. Artificially generated images comprised of particles of diameter in a range $1.1{\leq}d_p\leq10.0$ pixel were investigated. Three particle diameters were selected for detailed evaluation: $d_p$=2.2, 3.3, 4.4 pixel with a constant particle concentration 0.02 $particle/pixel^2$. Two flow patterns were considered: uniform and shear flows. The mean and random errors, and the computation times of the interpolation schemes were determined and compared.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1993.04a
• /
• pp.55-55
• /
• 1993

Farnesyl Protein transferase(FPT)는 발암유전자 ras의 단백질 산물인 p$^{21}$의 post-translational modification의 첫 단계인 ras-farnesylation에 관여하는 효소로 본 연구에서는 정제된 FPT와 E. coli에서의 발현 system을 이용하여 FPT의 구조와 기능을 밝히고 이를 FPT 방해제의 설계에 이용하고자 한다. Bovine testis에 존재하는 FPT를 30%-50%의 Ammonium sulfate로 fractionation하고, DEAE-Sephacel, Sephacryl S-300 column을 통과시킨 후 peptide(KKCVIM) affinity column을 이용하여 순수 정제하였다. 정제된 효소의 분자량은 gel-filtration에 의해 100KDa으로 추정되었고 SDS-PAGE 결과 49KDa과 46KDa의 두 subunit로 구성되었음이 확인되었다. 효소활성에는 $Mg^{2＋}$ 와 $Zn^{2＋}$가 필수적이며 최적 pH는 7.0이었다. Yeast의 FPT의 두 subunit 유전자는 Yeast genomic DNA를 template로 사용하고 각 subunit에 specific한 합성된 primer들과 vent polymerase를 이용하여 Polymerase chain reaction을 통하여 얻었다. 두 유전자를 pBluescriptII SK＋ vector를 변형시킨 두 vector, pBSK＋4와 pBChl＋4에 재조합 시킨 후 E.coli에 transformation시켜 발현시켰다. 현재 정제된 Bovine FPT와 E. coli에서 발현된 Yeast FPT의 chemical modification과 site-directed mutagenesis를 통하여 FPT의 active site와 substrate binding site에 관한 연구를 진행시키고 있다.

• Clean Technology
• /
• v.13 no.3
• /
• pp.215-221
• /
• 2007

Biosorption is considered to be a promising alternative to replace the present methods for the treatment of dye-containing wastewater. In this study, sewage sludge was used as a biosorbent which could be one of the cheapest and most abundant biomaterials. The objective of this work is to develop a surface-modified biosorbent with enhanced sorption capacity and binding affinity. The FT-IR and potentiometric titration studies revealed that carboxyl, phosphateand amine groups played a role in binding of dye molecules. The binding sites for reactive dye Reactive Red 4 (RR 4) were identified to be amino groups present in the biomass. In this work, based on the biosorption mechanism, the performance of biosorbentcould be enhanced by the removal of inhibitory carboxyl groups from the biomass for practical application of the biosorbents. As a result, the maximum capacity of biomass was increased up to 130% and 210% of the increment of sorption capacity at pH 2 and 4, respectively. Therefore, chemically modified sewage sludge can be used as an effective and low-cost biosorbent for the removal of dyes from industrial discharges.

• Korean Journal of Food Science and Technology
• /
• v.17 no.5
• /
• pp.383-388
• /
• 1985

Soy protein isolate was acylated with succinic anhydride and acetic anhydride. The functional properties are markedly improved by acylation of the $\varepsilon$-amino groups. Acylation of the available amino groups shifted the isoelectric point from 4.5 to 4.0 and enhanced the solubility between pH 4.0-6.0. In the 0.03M-$CaCl_2$ solution the solubility of the modified soy protein is much larger than that of the unmodified protein above the isoelectric point. The emulsion properties and foaming properties also improved by the modification and the effects of pH on the properties paralleled its effect on protein solubility. The changes of reduced viscosity with concentration followed Huggin's equation and by modification the intrinsic viscosity of the soy protein increased and the interaction coefficient decreased.

• Korean Journal of Optics and Photonics
• /
• v.15 no.4
• /
• pp.309-312
• /
• 2004

Two-dimensionally arrayed nanocolumn lattices were fabricated by using double-exposure laser holographic method. The hexagonal lattice was formed by rotating the sample with 60 degree while the square lattice by 90 degree before the second laser-exposure. The size and period of nanocolumns could be controlled accurately from 125 to 145 nm in diameter and 220 to 290 nm in period for square lattice by changing the incident angle of laser beam. The reactive ion etching for a typical time of 30 min using CH$_4$/H$_2$ plasma enhanced the aspect-ratio by more than 1.5 with a slight increase of the bottom width of columns.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2001.05a
• /
• pp.759-761
• /
• 2001

한반도 동해의 외해역(울릉도) 및 내해역에서(포항 및 묵호)의 조사된 T/P 해면자료와 TG해면자료간의 상관성이 각각 어떻게 나타나는가를 파악하기 위하여 본 연구를 수행하였다. 이때의 1992년 10월부터 1997년 12월까지 관측된 T/P자료와 TG자료를 각각 이용하였다. 본 연구로부터 우리는 다음과 같은 몇 가지 결과를 알 수 있었다. 1) 비교적 조석의 영향이 우세한 내해역에 위치한 포항 및 묵호가 외해역에 위치한 울릉도에 비해서 낮은 상관성을 보였다. 이러한 큰 차이는 M2, S2, Kl 분조 등에 의한 조석변형 때문에 발생하는 현상이다. 이것은 주로 T/P자료에 기인하는 조석오차이다. 2) 조석변형 때문에 발생하는 신호는 해양신호와 관계없는 잘못된 거짓신호로서 이를 제거하기 위해서는 lowpass filter를 적용하는 것이 좋다. 대체로 외해역의 경우는 60일, 120일, 180일, 200일의 필터를 순차적으로 처리함에 따라 상호 두 자료간의 유의성이 점차적으로 개선되는 것을 알 수 있었으나, 내해역의 경우에는 거의 유의성이 개선되지 않는 것으로 나타났다. 3) 200일의 필터를 처리했을 때, 외해역에 위치한 울릉도는 상관값이 0.91로 매우 높은 유의성을 보였고, 그리고 내해역에 위치한 포항 및 묵호는 각각 0.58 및 0.65로서 울릉도에 비해서 상대적으로 낮은 유의성을 보였다.

• Journal of the Korean Orthopaedic Association
• /
• v.56 no.6
• /
• pp.484-490
• /
• 2021

Purpose: A rheumatoid forefoot deformity includes severe hallux valgus and claw toe of the four lateral toes. The authors intended to analyze the mid-term results of the modified Hoffman procedure for a rheumatoid forefoot deformity. Materials and Methods: Twenty-two feet of eighteen patients were followed up for more than two years after the modified Hoffman procedure. The mean age was 54.7 years, and the mean follow-up period was three years and four months. Clinically visual analogue scale (VAS) and American Orthopaedic Foot and Ankle Society (AOFAS) MP-IP score were analyzed preoperatively and postoperatively. The postoperative complications, the satisfaction of patients, and the presence of plantar callosity were also analyzed. Radiographically, the hallux valgus angle, the 1st intermetatarsal angle, the 1st interphalangeal angle, the 1st interphalangeal joint arthritic change after the operation, and the dorsiflexion angle and the time to union of the 1st metatarsophalangeal joint were analyzed. Results: Clinically, the VAS and AOFAS score were respectively improved from 7.1 points and 30.3 points preoperatively to 1.5 points and 83.1 points postoperatively (p<0.001). All patients were satisfied with the results. Plantar callosity disappeared in all cases. Radiographically, the mean hallux valgus angle, the 1st intermetatarsal angle, and the 1st interphalangeal angle changed from 52.8°, 13.3°, and 7.5° preoperatively to 16.2°, 8.7°, and 14.6° postoperatively (p<0.001). The mean dorsiflexion angle of the 1st metatarsophalangeal joint was 17.2° postoperatively. The mean time to radiographic union of the 1st metatarsophalangeal joint was 11.1 weeks. There were two cock-up deformities of the lesser toe, one wound problem, and two hallux interphalangeal joint arthritis as a complication. There were no cases of nonunion of the 1st metatarsophalangeal joint. Conclusion: The modified Hoffman procedure appears to be a safe and satisfactory procedure for a rheumatoid forefoot deformity.

• Microbiology and Biotechnology Letters
• /
• v.26 no.1
• /
• pp.61-67
• /
• 1998

Recombinant human epidermal growth factor (rhEGF) was produced by E. coli BL2l (DE3) harboring a plasmid pYHB101. The production of rhEGF was 44.5 mg/L when the E. coli BL2l (pYHB101) was cultured at 27$^{\circ}C$ for 48 hr in the modified MBL medium containing 10 $\mu\textrm{g}$/L glucose with 10 $\mu\textrm{m}$ IPTG/lactose induction at 2 hr after inoculation. It was shown that lactose is able to induce the rhEGF expression of E. coli BL2l (pYHB101) with the same efficiency as IPTG. In the batch culture system, when induced with 10 $\mu\textrm{m}$ lactose, E. coli BL2l (pYHB101) produced maximum 45 mg/L of the rhEGF at 28 hr culture in the modified MBL medium containing 10 g/L glucose. In the semi-fed batch culture system, the volumetric yield was 160 mg/L when the culture was added with 0.5% (w/v) lactose and 0.25% (w/v) yeast extract in the late logarithmic phase and 94.3% of rhEGF was secreted as soluble form. However, when the culture was added with them in the early logarithmic phase, the volumetric yield was 120 mg/L and 20.9% of rhEGF was found in cytoplasmic insoluble aggregates. It was found that the addition time of lactose was important for production of soluble rhEGF from E. coli BL21 (pYHB101).

• Journal of Korean Ophthalmic Optics Society
• /
• v.12 no.1
• /
• pp.41-51
• /
• 2007

Differential equations and their solutions were formulated to describe the deflection of the tapered, nonuniform thickness and width's temple, clamped at one end while the perpendicular force is acting on the other end which is freely suspended. The model was derived based on laws of continuity at every point inside the elastic medium that the deflection, tangent slope, bending moment, shearing force must be continuous within the medium. The model is found to be in good agreement with measurements on the beta titanium temple with the correlation 0.992 and p=0.999(Chi test). Therefore it is possible to predict the effect of various temple parameters such as elastic modulus, thickness, width on the deflection of the temples being considered.

• Electrical & Electronic Materials
• /
• v.8 no.3
• /
• pp.291-297
• /
• 1995

The InP crystals have been grown by modified synthesis solute diffusion (SSD) method and its properties have been investigated. The crystals have been grown by lowering the crucible quartz for growth in the furnace and crystal growth rate is 1.8mm/day. The lattice constant a. of the grown crystals is 5.867.angs.. Etch pits density along growth direction of crystal changes from 3.0*10.sup 3/cm$\^$-2/ of first freeze part to 6.7*10$\^$4/cm$\^$-2/ of last freeze part and the radial direction of wafer shows nearly uniform distribution. The resistivity and the carrier concentration of the grown crystals are 1.43*10$\^$-1/.ohm.-cm, 7.7*10$\^$15/cm$\^$-3/ at room temperature, respectively. In the photolurninescence at 10K, the radiation transitions are observed by the near band edge recombination, a pair recombination due to Si donor - Zn acceptor and its phonon replica in the InP. The activation energy by Zn diffusion in undoped n-InP crystals is 1.22eV.