• Food Science and Preservation
• /
• v.22 no.6
• /
• pp.779-787
• /
• 2015

We stored sweet pumpkins at $8^{\circ}C$, $12^{\circ}C$, $12^{\circ}C$ plasma curing and room temperature (control) for 180 days. During this time, the quality characteristics were analyzed within the different groups. No spoiling occurred in either of the storage conditions for up to 120 days, and the marketability was good. After 120 days, spoiling sharply increased by over 70% in the control group, while in the $12^{\circ}C$ group it decreased to 20~60%. Conversely, spoiling was completely absent in the $8^{\circ}C$ and $12^{\circ}C$ plasma curing groups during the overall 180 days of storage. The lowest moisture content was found in the $8^{\circ}C$ group and the quality of the pumpkins was excellent during the entire storage period. The color of pulp was better in the $12^{\circ}C$ and $12^{\circ}C$ plasma curing groups, with best results were found in the latest. Both the $8^{\circ}C$ and $12^{\circ}C$ plasma curing groups maintained their dark green surface colors, while both the $12^{\circ}C$ and control groups turned from green to yellow. All groups showed a reduction in their initial hardness, with the $8^{\circ}C$ group staying the hardest. Soluble solid and mealiness was increased to the storage during 90 days while decreased, that were long to maintain the mealiness texture in the $8^{\circ}C$ storage pumpkin. The overall acceptability from sensory evaluation was higher in the $12^{\circ}C$ plasma curing group, when compared to those of the $8^{\circ}C$, $12^{\circ}C$ and control groups storage conditions.

• Korean Journal of Plant Resources
• /
• v.10 no.4
• /
• pp.401-410
• /
• 1997

This experiment was conducted to investigate the opimum media, media amount and planting density of nutri-culture for the high quality and yield of wild vegetable, Liguliaria fischeri Turez. The fried rice hull was recognized best media among perlite, sand and fried rice hull for nutri-culture of Ligularia fischeri Turcz. The yield on fried rice hull media at early growing stage was lower, but higher than in other media at late growing stage. Therefore, total yield in fried rice hull media was 15-29% higher than that of the existing perlite media. Growth and yield of Ligularia fischeri Turcz. were not significantly different between medium amounts, therefore considering economical efficiency and stability, the optimum medium amount was 105 $\iota/m^3$, Yield by planting density increased in the high density and 50 plants/$m^2$ was considered as proper planting density in nutri-culture of Liguliaria fishcheri Turcz.

• Korean Journal of Agricultural Science
• /
• v.4 no.1
• /
• pp.51-60
• /
• 1977

In order to determine ecological variations of flowering date and yields under the different seasonal cultures, and to select the higher yielding varieties which were adaptable to Korean climate, 100 mungbean cultivars were sown at the interval of 15 days from April 22 to July 21 in 1976. The results obtained are summarized as follows: 1. The number of days required to flowering from seeding were decreased by delaying the seeding date. 2. When accumulated temperature at first flowering from seeding were reached $945-1,126^{\circ}C$, the mungbean cultivars started to flower regardless of seeding dates. Especially, when mungbean was planted around standard planting date, the plants flowered for very short duration. 3. There were highly significant correlations between the number of days from seeding to flowering at each seeding date and standard planting date. 4. Yields per plant were tend to decrease with the delay of seeding date but there were no significant difference between seeding dates. Therefore, it appears that mungbean can be planted for longer period of time compared to other crops such as rice and soybean. 5. Highly significant correlations were found between the number of days of first flowering and yields per plant at most seeding dates. 6. It may be feasible to grow high yielding mungbean cultivars such as CES 140, LM 2100, LM 690, L 576 and LM 689 after harvesting of spring vegetables in May and before planting of fall vegetables.

• Journal of Sasang Constitutional Medicine
• /
• v.8 no.1
• /
• pp.187-246
• /
• 1996

The purpose of this study is to evaluate and standardize the four scales of Questionnaire for the Sasang Constitution ClassificationII (QSCCII). QSCCII is newly prepared by statistical item analysis and is designed to examine its diagnostic discriminability. QSCCII is administered to 1366 random informants. From the survey, we could get the data for the standardization. The criteria of standardization are based on the data from 265 informants who are examined by professionals. Collected data are analyzed by internal consistency, variation analysis(ANOVA), Duncan test and discrimination analysis of SPSS PC+ V4.0 program. The results are as follows 1) The reliability of four scales for QSCCII is relatively valid. The internal consistency of Tae-yang(太陽) scale is Cronbach's ${\alpha}=0.5708$. That of So-yang(少陽) scale is ${\alpha}=0.5708$. That of Tae-eum(太陰) scale is ${\alpha}=0.5922$. That of So-eum(少陰) scale is ${\alpha}=0.6319$. 2) There is a significant difference between each group through variation analysis of four scales. 3) The process of standardization is based on the average value and standard deviation with respect to age and sex difference of each criteria. 4) This study suggests a source of standardization of Sasang Constitution Classification by providing norms in which the differences of age, sex, and number of items are taken into deep consideration. QSCCII, therefore, can be applied to every age(the 10's to the 60's) and sex groups. 5) The recalculation of the raw-score to standard value (T-score) shows that the diagnostic discriminability (Hit-ratio : 70.08%) of QSCCII brings about 37% improvement than proportional chance criteria(33.33%). Especially, Hit-ratios of Tae-eum In(74.5%) and So-eum In(70.8%) are higher than that of So-yang In(60.0%). 6) QSCC has discriminability only to male informants. Compared with QSCC, however, QSCCII has relatively efficient discriminability both to male and female informants. 7) These results would be a demonstration of the fact that the QSCCII could be used as a tool for sasang constitution classification.

• Journal of Sasang Constitutional Medicine
• /
• v.8 no.1
• /
• pp.57-74
• /
• 1996

Donguisusaebowon, Kogchigo are wrritten by Dong-Mu. Dokhaengpyun of the Kogchigo has philosophy of Dong-Mu about Gi-In. In order to understand constitutional medicine of Dong-Mu, it is necessary to study about Bi-Bak-Tam-Ra'Ja(four types of man) in Dokhaengpyun. The results are summarized as follows : 1. In Dokhaengpyu, it is defined that In-Eui-Ye-JI - the nature of human - as In-Ja, Eui-Ja, Ye-Ja, Ji-Ja. This attribute is composed of Chung-Sin-Ip-Yong. We can know that this concept, that is Chung-Sin-Yeum-Hae'Ja, is relative to the Bi-Bak-Tam-Ra'Ja. 2. We can think and infer the relation of Sabujisim of Myeng-Ja and Bi-Bak-Tam-Ra'Ja from Bi-Bak-Tam-Ra'Bu that is attribute of In-Eui-Ye-ji'Ja. Bi-Bak-Tam-Ra'Ja can approach the behavior of Chung-Sin-Yeum-Hae'Ja, if they heard the attitude of YouHaHye and BaelYi which they have attribute of In-Eui-Ye-Ji'Ja. 3. They explain Tthat Bi-Bak-Tam-Ra-Jisim is quoted from SaBuJiSim of Dahak. Thinking that Bi-Bak-Tam-Ra-Jisim of Dokaengpyun and SaBujiSim of YuRiak, we know that there is no rule. So, it is difficult that we can infer the Simyok and make some pattern. 4. The relationship of Bi-Bak-Tam-Ra'Ja and In-Eui-Ye-Ji are different from the relationship of Dokhaegpyun, Sadanron, In-Eui-Ye-Ji of YuRiak and Bi-Bak-Tam-Ra'Ja. If we regard the In-Eui-Ye-Ji Sadan as the nature of human, many attritude can be possible and it may be different that apply a Sadan to the proper time and environment. Therefore, we have to be accepted change, as applying method than fixing idea.

• Journal of Life Science
• /
• v.25 no.6
• /
• pp.703-708
• /
• 2015

Hox genes encode a highly conserved family of homeodomain-containing transcription factors controlling vertebrate pattern formation along the anteroposterior body axis during embryogenesis. Retinoic acid (RA) is a key morphogen in embryogenesis and a critical regulator of both adult and embryonic cellular activity. Specifically, RA regulates Hox gene expression in mouse- or human-derived embryonic carcinoma (EC) cells. Histone modification has been reported to play a pivotal role in the process of RA-induced gene expression and cell differentiation. As histone modification is thought to play an essential role in RA-induced Hox gene expression, we examined RA-induced initiation of collinear expression of Hox genes and the corresponding histone modifications in F9 murine embryonic teratocarcinoma (EC) cells. Hox expression patterns and histone modifications were analyzed by semiquantitative RT-PCR, RNA-sequencing, and chromatin immuno-precipitation (ChIP)-PCR analyses. The Hoxc4 gene (D0) was initiated earlier than the Hoxc5 to –c10 genes (D3) upon RA treatment (day 0 [D0], day 1 [D1], and day 3 [D3]). The Hox nonexpressing D0 sample had a strong repressive marker, H3K27me3, than the D1 and D3 samples. In the D1 and D3 samples, reduced enrichment of the H3K27me3 marker was observed in the whole cluster. The active H3K4me3 marker was closely associated with the collinear expression of Hoxc genes. Thus, the Hoxc4 gene (D1) and all Hoxc genes (D3) expressed H3K4me3 upon transcription activation. In conclusion, these data indicated that removing H3K27me3 and acquiring H3K4me3 regulated RA-induced Hoxc gene collinearity in F9 cells.

• Journal of Life Science
• /
• v.25 no.3
• /
• pp.349-356
• /
• 2015

Thanks to recent advances in next-generation sequencing (NGS) technology, diverse livestock species have been dissected at the genome-wide sequence level. As for cattle, there are currently four Korean indigenous breeds registered with the Domestic Animal Diversity Information System of the Food and Agricultural Organization of the United Nations: Hanwoo, Chikso, Heugu, and Jeju Heugu. These native genetic resources were recently whole-genome resequenced using various NGS technologies, providing enormous single nucleotide polymorphism information across the genomes. The NGS application further provided biological such that Korean native cattle are genetically distant from some cattle breeds of European origins. In addition, the NGS technology was successfully applied to detect structural variations, particularly copy number variations that were usually difficult to identify at the genome-wide level with reasonable accuracy. Despite the success, those recent studies also showed an inherent limitation in sequencing only a representative individual of each breed. To elucidate the biological implications of the sequenced data, further confirmatory studies should be followed by sequencing or validating the population of each breed. Because NGS sequencing prices have consistently dropped, various population genomic theories can now be applied to the sequencing data obtained from the population of each breed of interest. There are still few such population studies available for the Korean native cattle breeds, but this situation will soon be improved with the recent initiative for NGS sequencing of diverse native livestock resources, including the Korean native cattle breeds.

• Journal of Life Science
• /
• v.22 no.11
• /
• pp.1493-1499
• /
• 2012

The level of genetic variation and relationships in three native Korean goat populations (Dangjin, Jangsu, and Tongyeong) as well as the populations of a farm were analyzed, based on 30 microsatellite markers. A total of 277 distinct alleles were observed across the four goat populations, and 102 (36.8%) of these alleles were unique to only one population. The mean observed heterozygosity and polymorphism information content were calculated as 0.461~0.651 and 0.462~0.679, respectively. In the NJ tree constructed based on Nei's $D_A$ genetic distance, the four populations represented four distinct groups. However, the genetic distances between each Korean native goat population and the farm population were two times those among the three native Korean breeds. The genetic structure within the three Korean native goat populations was also investigated. Cluster analysis, using the STRUCTURE software, suggested three clusters. The molecular information of genetic diversity and relationships in this study will be useful for the evaluation, conservation, and utilization of native Korean goat breeds as genetic resources.

• Journal of Life Science
• /
• v.19 no.11
• /
• pp.1658-1665
• /
• 2009

This study was carried out on thirty men to define the association of inflammatory markers with physiological factors on one-time aerobic exercise (for 15 min. Post-exercise interleukin-6 (IL-6), mean corpuscular hemoglobin concentration (MCHC), heart rate (HR), systolic blood pressure (SBP), and pulsatility and resistance index of middle cerebral artery (PI and RI, respectively) levels were elevated compared to those measured pre-exercise. Total leukocyte and platelet counts, high-sensitivity C-reactive protein (hs-CRP), free radical (FR), and low density lipoprotein cholesterol (LDL) levels tended to decrease after exercise. Pre-exercise IL-6 levels were positively correlated with pre-exercise SBP levels, while post-exercise IL-6 level was positively correlated with post-exercise PI and RI levels. Post-exercise, hs-CRP levels were negatively related to SBP and HR. Pre-exercise, FR levels were positively associated to SBP, DBP, and HR. Post-exercise FR levels were negatively related to the post-exercise blood flow velocity in middle cerebral artery. Pre-exercise erythrocyte indices (RBC, MCV, MCH, and MCHC levels) were in inverse proportion to pre-exercise IL-6 levels. Post-exercise FR levels were inversely related to post-exercise total leukocyte, lymphocyte, monocyte, and MCH levels. Pre-exercise $Mg^{++}$ levels were in inverse proportion to pre-exercise IL-6, hs-CRP, or FR levels. These findings suggest that one-time aerobic exercise offers a significant relationship between inflammatory markers and some biochemical markers or electrolytes. Further studies need to be carried out for investigation of differences between genders or age groups following one-time or regular aerobic exercise.

• Journal of Life Science
• /
• v.23 no.5
• /
• pp.710-720
• /
• 2013

Radiotherapy is commonly used in treating many kinds of cancers which cannot be cured by other therapeutic strategies. However, radiotherapy also induces the damages on the normal tissues. Radiation-induced fibrosis is frequently observed in the patients undergoing radiotherapy, and becomes a major obstacle in the treatment of intrahepatic cancer. Hedgehog (Hh) that is an essential in the liver formation during embryogenesis is not detected in the healthy liver, but activated and modulates the repair process in damaged livers in adult. The expression of Hh increases with the degree of liver damage, regulating the proliferation of hepatic progenitors and hepatic stellate cells (HSC). In addition, Hh induces epithelial-to-mesencymal transition (EMT) and activation of myofibroblasts. In the irradiated livers, up-regulated expression of Hh signaling was associated with proliferation of progenitors, EMT induction, and increased fibrosis. Female-specific expression of Hh leaded to the expansion of progenitors and the accumulation of collagen in the irradiated livers of female mice, indicating that gender disparity in Hh expression may be related with radiation-susceptibility in female. Hence, Hh signaling becomes a novel object of studies for fibrogenesis induced by radiation. However, the absence of the established experimental animal models showing the similar physiopathology with human liver diseases and fibrosis-favorable microenvironment hamper the studies for the radiation-induced fibrosis, providing a few descriptive results. Therefore, further research on the association of Hh with radiation-induced fibrosis can identify the cell and tissue-specific effects of Hh and provides the basic knowledge for underlying mechanisms, contributing to developing therapies for preventing the radiation-induced fibrosis.