• Title/Summary/Keyword: 변이성

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Selection of High Tobramycin-Producing Mutants (Tobramycin 고생산성 변이주의 분리)

  • 나규흠;김학주;김기태;양중익;김계원
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.343-347
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    • 1991
  • An improved method for the selection of high tobramycin-producing mutants of Streptomyces tenebrarius ATCC 17920 was investigated. By the use of apramycin-containing media, low nebramycin-producing mutants were eliminated. Strain No. 23, resistant to apramycin and kanamycin B and sensitive to tobramycin, was isolated from soils, identified as Pseudomonas paucimobilis and used as a test organism for overlaying the mutants on agar plate. While inhibition zones were not shown when the parent strains were overlaid with soft agar containing the strain No. 23, clear zones were shown when high tobramycin-producing mutants were overlaid. Using this screening strategy, 58 mutants showing clear zones had been isolated. antibiotic activities of which were incresed to 3~8 fold compared to that of parent strain.

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Flexion im Koreanischen - Allomorphie und Konkatenation - (한국어의 굴절 : 변이와 상호결합)

  • Kim Soora
    • Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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    • v.3
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    • pp.185-209
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    • 2001
  • 필자는 이 논문에서 LA-MORPH 개념에 바탕을 둔 한국어 어형 분석시스템을 구현하기 위한 사전 작업으로서 한국어 동사의 활용 현상을 기술하였다. 한국어 동사 활용의 특징인 동사의 다형성은 크게 두 가지 측면으로 나눠서 관찰할 수 있다. 하나는 동사 및 어미의 변이형 형성과 관련된 측면이고 다른 하나는 이들 동사 및 어미 변이형의 상호 결합에 관련된 측면이다. 관찰의 출발점은 동사가 구체적으로 실현되어 나타나는 단어 형태이다. 규칙 활용 및 불규칙 활용 동사의 실현 형태를 어간 및 어미로 분절하고 이 작업을 통해 얻어진 동사 및 어미의 변이형과 그 기본형 사이에 존재하는 규칙성을 토대로 변이형 유도 모형을 유형별로 제시하였다. 어미를 어간과의 결합 유형에 따라 세 가지로 분류하고 각각에 대해 동사 변이형과의 결합 양상을 제시하였다. 한국어 정서법의 특성을 고려함으로써 동사 활용현상 중 특히 동사 변이형 및 어미 변이형들 간의 결합 양상 및 규칙 등 기존 문법 및 사전이 완전히 기술하지 못하는 부분을 보완할 수 있었다. 더 나아가 'ㄹ' 불규칙 활용, '으' 불규칙 활용 등 기존 문법에서 불규칙적이라고 간주되는 활용 현상에도 일정한 정도의 규칙성이 존재함을 보였다.

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벼 도열병 저항성 변이체 선별

  • 김혜경;조지연;이상규;홍연규;이기환;이선우;최경자;이시철;안진흥
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.04a
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    • pp.127-127
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    • 2003
  • 벼 도열병 저항성 조절과정을 연구하는 효율적인 방법은 돌연변이를 분리하여 해당유전자를 분석하는 것이다. 이를 위하여 벼에서 제조된 4가지 돌연변이 집단을 이용하여 도열병 저항성 돌연변이를 분리하였다. 실험재료로는 1) fast neturon을 처리하여 제조된 Moroberekan 2,000 라인, 2)T-DNA의 형질전환에 의해서 제조된 화영 1,000 라인, 3)DEB처리에 의해서 제조된 RIL260 3,000라인, 4) gamma ray 처리에 의해서 제조된 상해향혈나 10,000 라인 등이 사용되었다. 병 저항성이 감소된 돌연변이의 분리를 위하여 재료로 사용된 벼 품종과 비친화적 상호작용을 보이는 균주의 포자를 2-3주된 벼 잎에 직접 살포하는 방법을 이용하였다. 균주 접종 7 일 후에 blast lesion을 형성하거나 lesion mimic 표현형을 보이는 돌연변이 등 병저항성이 감소된 라인을 선별하였다. 현재까지 1) Moroberakan 5 라인, 2) 화영 4 라인, 3) RIL260 1 라인 등이 선별되었다. 이와 함께 병저항성이 현저히 증가된 돌연 변이를 선별하기 위하여 친화적인 균주를 사용한 실험에서는 상해향렬나 2 라인이 선별되었다. 선별된 돌연변이는 벼 도열병 저항성 유전자의 분리 및 저항성 조절기작을 연구하는데 효과적으로 사용될 것이다.

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Inhibitory Effect of Yam (Dioscorea batatas DECENE) Extracts on the Mutagenicity (돌연 변이원에 대한 마(Dioscorea batatas DECENE)추출물의 억제 효과)

  • 이임선;정세영;신창섭;구성자
    • Korean journal of food and cookery science
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    • v.11 no.4
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    • pp.351-355
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    • 1995
  • The inhibitory effects of methanol, ethanol, chloroform/ methanol and water extracts from natural and cultural yams on the mutagenicity in the cooked pork (broiled and panfried) and the chemically induced mutagen, sodium azide, benzo(a)pyrene and 2-aminofluorene were investigated using salmolla typhimurium TA 100. In the presence of the S9 mixture, ethanol extract from natural yam showed high inhibitory effect on the mutagenicity of the cooked pork. But benzo(a)pyrene, supposed to be produced in mutagen during cooking, did not show high inhibitory effect in same extract. Besids, the yam extract on the mutagenicity of the sodium azide without S9 mixture showed low inhibitory effect. However 2-aminoflourene with S9 mixture showed high inhibitory effect, 91.5%.

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Spatio-tempers Change Prediction and Variability of Temperature and Precipitation (기온 및 강수량의 시공간 변화예측 및 변이성)

  • Lee, Min-A;Lee, Woo-Kyun;Song, Chul-Chul;Lee, Jun-Hak;Choi, Hyun-Ah;Kim, Tae-Min
    • Spatial Information Research
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    • v.15 no.3
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    • pp.267-278
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    • 2007
  • Internationally many models are developed and applied to predict the impact of the climate change, as occurring a lot of symptoms by climate change. Also, in Korea, according to increasing the application of the climate effect model in many research fields, it is required to study the method for preparing climate data and the characteristics of the climate. In this study IDSW (Inverse Distance Squared Weighting), one of the spatial statistic methods, is applied to interpolate. This method estimates a point of interest by assigning more weight to closer points, which are limited to be select by 3 in 100 km radius. As a result, annual average temperature and precipitation had increased by $0.4^{\circ}C$ and 412 mm during 1977 to 2006. They are also predicted to increase by $3.96^{\circ}C$, 319 mm in the 2100 compared to 2007. High variability of temperature and precipitation for last 30 years shows in some part of the Gangwon-do and in the southern part of Korea. Besides in the study of the variable trend, the variability of temperature and precipitation is inclined to increase in Gangwon-do and southern east part, respectively. However, during 2071 to 2100 variability of temperature is predicted to be high in midwest of Korea and variability of precipitation in the east. In the trend of variability, variability of temperature is apt to increase into west south, and variability of precipitation increase in midwest and a part of south.

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Fast Disparity Estimation Method Considering Temporal and Spatial Redundancy Based on a Dynamic Programming (시.공간 중복성을 고려한 다이내믹 프로그래밍 기반의 고속 변이 추정 기법)

  • Yun, Jung-Hwan;Bae, Byung-Kyu;Park, Se-Hwan;Song, Hyok;Kim, Dong-Wook;Yoo, Ji-Sang
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.10C
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    • pp.787-797
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    • 2008
  • In this paper, we propose a fast disparity estimation method considering temporal and spatial redundancy based on a dynamic programming for stereo matching. For the first step, the dynamic programming is performed to estimate disparity vectors with correlation between neighboring pixels in an image. Next, we efficiently compensate regions, which disparity vectors are not allocated, with neighboring disparity vectors assuming that disparity vectors in same object are quite similar. Moreover, in case of video sequence, we can decrease a complexity with temporal redundancy between neighboring frames. For performance comparison, we generate an intermediate-view image using the estimated disparity vector. Test results show that the proposed algorithm gives $0.8{\sim}2.4dB$-increased PSNR(peak signal to noise ratio) compared to a conventional block matching algorithm, and the proposed algorithm also gives approximately 0.1dB-increased PSNR and $48{\sim}68%$-lower complexity compared to the disparity estimation method based on general dynamic programming.

Mutagenic Test of Gardenia Yellow Pigment (치자 황색색소에 대한 변이원성 시험)

  • 김희구
    • The Korean Journal of Food And Nutrition
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    • v.11 no.1
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    • pp.72-76
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    • 1998
  • Gardenia yellow pigment produced by Gardenia jasminoides Ellis was tested for reverse mutagenic test in Salmonella typhimurium stains TA1535, TA1537, TA98 and TA100 at concentrations raging form 6.25 to 200$\mu\textrm{g}$/$m\ell$ per plate. No significant reverse mutagenic activity was observed in any of the S. typhimurium strains, in either presence or absence of S9 mix. There was no toxicity to the bacteria. These result indicate that yellow pigment doesn't have mutagenicity.

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A Classifier for the association study between SNPs and quantitative traits (SNP와 양적 표현형의 연관성 분석을 위한 분류기)

  • Uhmn, Saangyong;Lee, Kwang Mo
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.11
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    • pp.141-148
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    • 2012
  • The advance of technologies for human genome makes it possible that the analysis of association between genetic variants and diseases and the application of the results to predict risk or susceptibility to them. Many of those studies carried out in case-control study. For quantitative traits, statistical analysis methods are applied to find single nucleotide polymorphisms (SNP) relevant to the diseases and consider them one by one. In this study, we presented methods to select informative single nucleotide polymorphisms and predict risk for quantitative traits and compared their performance. We adopted two SNP selection methods: one considering single SNP only and the other of all possible pairs of SNPs.

The screening of the mutagenicity and desmutagenicity for cooked pork meats according to cooking conditions (조리 조건을 달리한 가열조리된 육류(돼지고기)의 변이원성 검색과 제어에 관한 연구)

  • 정경숙;구성자
    • Korean journal of food and cookery science
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    • v.18 no.6
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    • pp.716-722
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    • 2002
  • Meat(beef, pork, chicken, duck) were cooked by three kinds of instruments (gas grill. electric grill, microwave oven) and extracted with 80% methanol. These methanol extracts were performed the Ames test, employing S. typhimurium tester strain TA100 (in vitro) and micronucleus test (in vivo). The methanol extract of cooked pork showed high mutagenicity in 5.0mg/plate without S9 mix and induced a higher mutagenicity with S9 mix than without S9 mix at 5 mg/plate. In all kinds of cookery methods, pork extracts showed high mutagenicity according to increase of cookery temperature (200$\^{C}$, 260$\^{C}$ and 320$\^{C}$). The methanol extract of cooked pork by electric grill (at 260$\^{C}$, for 5 min) showed high mutagenicity in all kinds of cookery instruments on the Ames test and micronucleus test. In all kinds of meat, the methanol extract of cooked pork showed a higher mutagenicity than the others and chicken showed a lower. The extract after pork soaked in ginger juice showed lower mutagenicity and micronucleus formation than the other vegetable juice.

On the strong attractive force dependence of the surface phase transition : Qualitative consideration from the occupation statistics (표면상변이의 강인력 의존성 : 점유통계를 이용한 정성적 고찰)

  • 김철호
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.4
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    • pp.573-577
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    • 1997
  • Surface phase transition is known to be observed at the system that there exists a strong attractive potential between particles adsorbed. This study presents a dependence of the surface phase transition on strong attractive force using a simple occupation statistical method. It was found that the system exhibits a phase transition from the vacuum phase into the population phase and the critical pressure also increases with the temperature. This fact indicates that these results explain well qualitatively the surface phase transition.

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