• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.53-62
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• 2012

Silkworm larvae often suffer from viral infections causing heavy losses to the economy of silk industry. Insects exhibit both humoral and cellular immune responses that are effective against various pathohens like bacteria, fungi, protozoa, etc., but no insect immune responses is effective against viral infection. To obtain genes related to insect antiviral immunity from Bombyx mori, the cDNA library was constructed from B. mori nucleopolyhedrovirus (BmNPV)-infected B. mori. From the cDNA library, we selected 411 differentially expressed clones, and the 5' ends of the inserts were sequenced to generate ESTs. In this work, 135 unigenes were generated after the assembly of 411 differentially expressed clones ESTs. Of these 135 unigenes, we selected 109 antiviral response-related candidates except 26 clones that high similarity with genes derived from BmNPV. Among 109 unigenes, a total of 80% had significant matches to genes from other organisms in the database, wheres 20% of the unigenes had not matched in the database. Functional groups of these sequences with matches in database were constructed according to their putative biological function. Three largest categories were control of cellular oraganization (52%), metabolism (20%), and protein fate (10%). The genetic information reported in this study will provide more information about antiviral-related genes in silkworms.

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.16-26
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• 1995

DNA restriction fragment length polymorphisms(RFLPs) were used for the classification of 22 leading silkworm races and wild silkworm, Bombyx mandarina. A genomic DNA library from silkworm was partially constructed and was prescreened to evaluate the selected DNA probes. Three DNA probes (SP1-13, SP1-28, 10-42) were selected to determine the polymorphism between silkworm races. As a result, high polymorphism with the probe SP1-28, moderate polymorphism with SP1-13 and monomorphism with 10-42 were obse-rbed.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.33-36
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• 2001

This study was carried out to improve the liquid culture methods of Paecilomyces japonica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150rpm under the photoperiod of 12L-12D. Dry weight of mycelium was averagely 1.216 g in the Sikworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6 g of 6 mmbeads in the SL medium, the dry weight is increased to 1.332 g. The optimal addition of silkworm larval powder to the culture medium for gest harvest was 1.360$\pm$0.67 g in dry weight.

• Journal of Sericultural and Entomological Science
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• v.34 no.2
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• pp.71-74
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• 1992

우리나라 양잠의 안정적 성장 발전을 위해서는 생산기반의 재정비 및 호당 양잠 규모의 확대가 필요하다고 하는 것은 누구나 다 아는 사실이다. 따라서 이를 위한 생산체계의 획기적인 탈바꿈의 하나로 애누에 공동육을 함으로써 농가에서 누에의 사육기간을 단축하는 것이 시급히 요청된다고 림은 "잠사업이 국제 경쟁력 제고를 위한 기술적 대응방안"에서 주장한 바가 있었다. 1. 전제조건 1) 애누에 공동육이 꼭 이루어져야 한다. 2) 뽕밭 생산성이 저해 받아서는 안 된다. 3) 성력 기계기구 개발 및 지원이 이루어져야 한다. 4) 가장 중요한 것은 잠작안정이다. 5) 각 잠기별 표준관리된 잠종이 공급되어야 한다. 6) 각 수견 시기에 맞추어 고치공판이 이루어져야한다. 2. 누에 사육시간 선정 1) 뽕나무 생리를 감안한 가지뽕 수확법에 따른 누에 사육기간은 춘잠기는 5월 하순부터 6월 중순까지이고 추잠기는 9월 초순부터 하순까지이다. 2) 병충해 방제관리상으로는 하잠 및 초추잠만이 문제점이 있다. 3) 잠작에 따른 사육기간은 춘잠기는 5월 하순부터 6월 중순까지이며 추잠기는 9월 초순부터 중순까지잠작이 양호하다.(중략)

• Journal of Sericultural and Entomological Science
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• v.39 no.1
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• pp.30-35
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• 1997

A rapid and sensitive detection of BmNPV contamination in silkworm rearing room was carried by Plymerase chain reaction(PCR). Silkworm nuclear polyhedra were dissolved for the extraction of viral DNA within 30 minutes followed by the treatment of alkaline solution. The combination of primers of NP3 and NP2 was superior in PCR to the other 7 primers applied. Each primer was designed with 20 base in size and Newly designed NP3 of sense and the already reported NP2 for antisense were better in reaction than other primers. PCR products appeared 500bp in size. And annealing was confirmed proper at 55$^{\circ}C$ condition. Amplifiable template DNA amount was confirmed at least 100 ng to 0.1 ng and regarded as applicative for the assay of silkworm rearing environmental condition of sericultural farm. In case of the detection of BmNPV from the dust, sensitivity by PCR was as high as 1,000,000 times than that of microscopic observation.

• Journal of Sericultural and Entomological Science
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• v.29 no.1
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• pp.53-56
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• 1987

The effect of nematicides on root-knot nematode were investigated in the mulberry nursery farm of Jeonnam province from 1985 to 1986. The nematicides applied were Fenamiphos, D-D(92%), Aldicarb, D-D(55%), Ethoprop and Carofuran. The control effects of nematicides used were similar in two years (1985 and 1986). The Fenamiphos and D-D(92%) both were a highly effective than any of Aldicarb, D-D (55%) and Ethoprop, Carbofuran. Mulberry sampling showed good growth when the above nematicides were applied.

• Journal of Sericultural and Entomological Science
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• v.41 no.3
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• pp.205-210
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• 1999

Silk nonwoven fabrics are prepared by needle punching and thermal bonding with silk waste. To enhance the carding efficiency, the degumming rate was controlled with sodium hydrogen sulfite solution. The amount of the remained sericin was 3%(S-3), and 6%(S-6). Mixing wool and LMP(Low melting polyester) with the silk, to improve carding efficiency, was also effective. Following items were tested with prepared silk nonwovon fabrics : weight, thickness, compression, tensile strength, heat insulation, water absorption, and deodorization. The results show that the silk nonwoven fabrics could be used for apparels and new biomaterials.

• Journal of Sericultural and Entomological Science
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• v.45 no.1
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• pp.66-70
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• 2003

Silk fabrics were dyed with persimmon dyeing solution. The persimmon extracts were obtained with boiling water from persimmon fruits and leaves. The changes of K/S values were investigated according to the concentration of solution, the ripeness of persimmons and the method of mordanting. The colours of the fabrics differed according to the kinds of mordants. The mordant, FeSO$_4$, was more effective than other mordants. The physical properties were not changed hardly after the natural dyeing with persimmon extracts.

• Journal of Sericultural and Entomological Science
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• v.32 no.1
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• pp.44-48
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• 1990

Monoclonal antibodies were prepared against Bombyx densonucleosis virus type-II(Yamanashi isolate). Four hybridoma clones, named C4, Fl, H2, M9 were only reacted with the DNV-II, but those were not reacted with Bombyx densonucleosis virus type-I(Ina isolate) and infectious flacherie virus(IFV) by double diffusion test in 0.8% agarose gel. C4, Fl and M9 of them were reacted with 53KDa polypeptide of DNV-II, and H2 was reacted with 46. 5KDa polypeptide of the virus.

• Journal of Sericultural and Entomological Science
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• v.19 no.1
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• pp.9-13
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• 1977

This experiment was carried out to know relationship between economical characters and kinds of extracted cellulose powders, extracting methods and cellulose composition. The results obtained are summarized as follows; 1. Among the matters for cellulose resources, mulberry branch is the most useful in economical characters such as larval weight, ratio of cocoon shell and mortality, followed by pine branch. 2. Though both PA-method and NaOH-treatment are equally effective in the larval growth, the latter may be more recommendable because of its economical convenience. 3, It may be desirable to add 15 to 22.5 per cent of cellulose powder per dry matter in the diet composition