• Proceedings of the Korean Society of Environment and Ecology Conference
• /
• 2009.04a
• /
• pp.41-42
• /
• 2009

• Journal of Animal Science and Technology
• /
• v.51 no.3
• /
• pp.255-262
• /
• 2009

Current study was conducted to investigate the influence of parity and region variation in ultrasound live carcass traits of Hanwoo cows. A total of 1,386 heads of Hanwoo cows, spreading over six regions in Gangwon province of South Korea, were measured for longissimus muscle area, back fat thickness, marbling score and rump fat thickness by using real-time ultrasound method. The results revealed that, positive correlations were found in all ultrasound live carcass traits. All live carcass traits were increased in accordance with parity until the sixth, and then decreased thereafter. Statistical significant differences (p<0.01) were found among regions in ultrasound live carcass traits, e.g. Hanwoo cows showed highest longissimus muscle area and marbling score in Hwacheon, while the lowest back fat thickness and rump fat thickness were observed in Yanggu. Greater longissimus muscle area and marbling scores were found in Hanwoo cows which were born during autumn, 2000. Moreover, individuals born during autumn, 2005 and 2006 showed a thicker back fat and rump fat thickness. Back fat thickness had the highest positive correlation with rump fat thickness, while rib eye area and rump fat thickness had the lowest correlation.

• Korean Journal of Animal Reproduction
• /
• v.16 no.1
• /
• pp.1-6
• /
• 1992

These experiments were undertaken as a basic study to understand the role of cumulus cell on in vitro maturation and fertilization process with identifying the cumulus cell-secreted proteins. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and fast protein liquid chromatography(FPLC), the proteins of cumulus cells were identified. The results obtained in these experiments were summarized as follows ; 1. When the proteins secreted from cultured cell for 30 hours were separated by SDS-PAGE, there were a major band (>94,000) and other minor 2 bands with molecular weight ranging 30,000∼67,000 dalton. 2. In addition, the fractionations of these proteins by FPLC were idirectly shown that three bands were hyaluronic acid, chondroitin sulfate, and heparin.

• Korean Journal of Animal Reproduction
• /
• v.15 no.2
• /
• pp.149-155
• /
• 1991

본 실험은 H-Y 항원 유전자 크로닝을 위한 기초연구로서 H-Y 항원의 특성을 규명하기 위하여 친화성 크로마토그래피에 의하여 H-Y 항원을 분리·정제하였다. 정소 추출액을 항체가 결합된 column에 결합시킨 뒤 10% acetic acid로 용출시켰다. 용출된 분획을 모아 농축한 후 HPLC와 SDS-PAGE를 실시하여 H-Y 항원의 분자량은 약 6,7000달톤 임을 알 수 있었으며 isoelectric focusing에 의하여 등전점(pI)은 5.0인 것으로 측정되었다. H-Y 항원에 대한 단일클론항체와 표지항원으로는 H-Y 항원-ABEI(aminobutylethyl isoluminol)를 사용하여 H-Y 항원 정량을 위한 화학발광면역분석법을 개발하였다. 항원항체 반응후 빛의 측정은 NaOH 존재하에서 microperoxidase/H2O2를 이용한 산화반응으로 실시하여 10초간 측정한 빛의 양을 적분하였다. H-Y 항원의 농도와 빛의 양과는 역비례하였으며 감도는 11.8ng/tube 정도이었다.

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.203-203
• /
• 2004

조류는 발생학적 특성상 수정과 초기 배발생을 제외한 거의 대부분의 개체 발생 과정이 난각 속에서 진행된다. 또한 배자가 발생하는 동안 배자의 뼈를 구성하는 석회성분의 거의 대부분은 난각에서 유래한다. 난각 석회성분은 배자유래의 융모성 뇨막(chorioallantoic membrane; CAM)의 모세혈관을 통하여 배자로 이동하다. 배자 발생후기로 가면 융모성 뇨막은 전체 난각과 거의 대부분 밀착하여 배자 발생 후기에 필요한 많은 양의 석회성분을 난각에서 배자로 공급하게 된다. 그러므로 한 개의 난각 속에서 두 개의 배자가 정상적으로 발생되기란 거의 불가능하다. (중략)

• Korean Journal of Veterinary Research
• /
• v.44 no.1
• /
• pp.105-111
• /
• 2004

A total of 301 Thoroughbred mares were investigated the characteristics of breeding in the local areas in Jeju during the period from February 2000 to July 2001. The pregnancy rate and foaling rate in 143 mares were 93.0%, 72.0%, respectively, and mean gestational length was 339.3 days in 2000. The pregnancy rate in 158 mares was 86.7% in 2001. Mature follicles were ovulated in right(46.0%), left(45.2%), and bilateral(7.8%) ovary. The incidence of multiple pregnancies in 158 mares between 15 days and 45 days after mating were 89.3%(single), 10.1%(twin), and 0.6%(triple). The incidence of EED was 13.3%. Also uterine cyst and fluid had an bad influence on mare's pregnancy. These results suggest that regular monitoring of the pregnancies and maintaining of good uterine condition improve the pregnancy rates and foaling rates during breeding season.

• Proceedings of the KAIS Fall Conference
• /
• 2011.12a
• /
• pp.286-288
• /
• 2011

활성탄소섬유의 유해 미생물의 번식 억제력을 조사하기 위해 한천베지배양법을 이용하였다. 전이금속으로 코팅된 활성탄소섬유는 일부 유해 미생물에서 번식억제력이 나타났으며, 유해 미생물은 인간의 피부 부종을 야기시키는 미생물인 candida albicans이었다. 그리고 코팅 활성탄소섬유의 흡 탈착력은 코팅되지 않은 활성탄소섬유보다 약 5% 내외로 감소함을 알 수 있었다.

• Korean Journal of Animal Reproduction
• /
• v.14 no.2
• /
• pp.125-131
• /
• 1990

These experiments were carried out to investigate of the molecular characteristics of porcine zona pellucidae and to examine the reactivity of anti-zona antibody by SDS-PAGE, Immunoblotting and Immunoprecipitation. The results obtained in these experiments were summarized as follows : 1. The result obtained by SDS-PAGE of porcine zona pellucidae indicated that it composed of several units with molecular weight ranging 55,000-110,000. 2. In order to see the reactivity of antibodies to zona pellucidas, immunoblotting was applied. The results indicated that polyclonal antibodies to porcine and mouse zona reacted with porcine zona. While monoclonal antibody to porcine did not react with the procine zona enough to show a clear band on a gel. 3. Labelling of porcine zonae with 125I was performed using the Iodogen method, the radioactivity and the percent incorporation of 125I into porcine zonae were approximately 26,000 cpm/10${mu}ell$ and 16, respectively. 4. Measurements of radioactivity and O.D value for Immunoprecipitates produced by reaction of 125I-porcine zona with anti-zona antisera were confirmed that existence of reactivity of monoclonal antibody to porcine zona although its reactivity was lower than that of polyclonal antibodies, and reconfirmed that cross-reactivity of polyclonal antibody of mouse zona with porcine zona.

• Korean Journal of Environment and Ecology
• /
• v.33 no.6
• /
• pp.636-644
• /
• 2019

The purpose of this study was to investigate the callings of nocturnal birds using bioacoustic recording technology to identify species and to analyze the ecological characteristics of each species. Three sites - Seoraksan National Park, National Institute of Ecology, and Mudeungsan National Park - were investigated. The investigation period was from the middle of April 2018 to early March 2019 for Seoraksan national park, from late February of 2018 to the middle of February 2019 for the National Institute of Ecology, and from the middle of February 2018 to the end of August 2018 for Mudeungsan National Park. The main research results are as follows. Firstly, nocturnal bird species identified by the survey included Caprimulgus indicus, Otus sunia, Zoothera aurea, Bubo bubo, and Strix uralensis, 5 species in total. Secondly, the breeding call period of each species was from early May to early August for C. indicus, from early April to the end of September for O. sunia, from early March to early October for Z. aurea, from late September to early February for B. bubo, and from mid-January to early March for S. uralensis. Thirdly, the mating call rhythm was between 16:00 and 10:00 on the following day for all the observed species in the three regions, and the peak time zone was from 20:00 to 06:00 on the following day. Fourthly, there was no correlation between the cumulative call frequency and the precipitation for each species. Fifthly, the mean temperature during the period when the specific calls of nocturnal birds were detected was -4.00 ℃ for S. uralensis, 2.58 ℃ for B. bubo, 13.66 ℃ for Z. aurea, 19.50 ℃ for O. sunia, and 20.77 ℃ for C. indicus. The ANOVA results showed that there was a significant difference in mean temperature for the calling by species and that the mean temperature was S. uralensis, B. bubo, Z. aurea, and O. sunia-C. indicus, in the ascending order, for 4 groups in total. The period of the specific mating calls confirmed by the study is a period in which the frequency of calls was the highest among the periods when the specific calls were detected. Since it is associated with the known mating period of each species, the period of the high frequency of calls confirmed by the bioacoustic monitoring can be regarded as the mating season. This study is meaningful in that it is the early research that has used the bioacoustic recording technology to identify species and ecological characteristics of species of nocturnal birds in Korea.

• Korean Journal of Environment and Ecology
• /
• v.28 no.3
• /
• pp.273-280
• /
• 2014

This study examined whether Dybowski's brown frogs(Rana dybowskii) in noisy highway roadsides had different mating calls from those in natural sites. We selected four study sites: two rice paddy sites in Youngdong Highway roadside and two nearby natural counterparts. Frog calls were recorded between 18:30 and 20:00 on February 24, February 27, and March 14, 2014. Frog calls in the natural sites had fundamental frequency approximately at 700 Hz with two to four apparent harmonics, while frog calls in the highway sites had higher fundamental frequency with up to seven apparent harmonics. Analysis of variance (ANOVA) shows that a roadside site that are directly exposed to highway noise had statistically higher frog calling frequency than other study sites. However, the higher calling frequency was not found in another roadside site that differed in elevation from the highway and was buffered by forests. These results indicate that male frogs in a noisy highway roadside called females with a higher pitch and more apparent harmonics to avoid being masked by highway noise. These results also suggest that there is a threshold noise level that interrupts frog's mating behavior and it is needed to maintain highway roadside noise to this threshold level.