• Clinical and Experimental Pediatrics
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• v.48 no.5
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• pp.545-550
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• 2005

Purpose : Newborn brain tissue has to be dissociated into a single cell suspension for flow cytometric analysis of cell death during hypoxia-ischemia. Thus the development of a method to dissociate cells from the brain tissue with least damage and maintenance of membrane and antigen integrity remains the challenge for the in vivo application of this technique. We evaluated the efficacy of mechanical or enzymatic (collagenase or tryspin) methods of brain tissue disaggregation. Methods : The extent of the damage to the plasma membrane and loss of the characteristics of the membrane induced with each dissociation method was determined by comparing the flow cytometric results labeled with both fluorescent annexin V and propidium iodide of the newborn rat pup brain tissue in the control group (n=10) and in the 48-hour after hypoxia-ischemia group (n=10). Results : In the control group, the cell percentage of damaged, apoptotic and necrotic cells of both hemispheres with the mechanical dissociation method was significantly increased compared to the trypsin or collagenase method. In the 48-hour after hypoxia-ischemia group, the cell percentage of apoptotic and necrotic cells of the right hemisphere with the collagenase method significantly increased, and live cells significantly decreased compared to the left hemisphere, control group. Although the same trend was observed, the extent of alterations made with the trypsin method was significantly less compared to the collagenase method. Conclusion : The dissociation of neonatal brain tissue for flow cytometric analysis with collagenase was most efficacious with the least cell damage and preservation of the plasma membrane characteristics.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.3
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• pp.218-225
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• 2005

본 연구의 목적은 치아 회분말과 연석고 및 알긴산을 혼합하여 이식한 후 초기 골 형성을 알아보는 데 있다. 백서 30마리의 두개골에 8mm 직경의 골결손부를 형성한 후 인간의 치아 회분말과 연석고 및 알긴산을 혼합하여 이식한 후 대조군, 치아 회분말과 연석고 처치군, 그리고 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 각각의 골 결손부 치유과정을 조직병리학적, 조직형태계측학적으로 비교 관찰하였다. 통계학적으로 주간 변화(4주, 8주)는 유의하지 않았고, 각 그룹 간에 있어도 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군 간 모두(4주, 8주) 유의한 차이가 없었다. 그러나 이들 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군은 대조군에 비해서는 유의하게 우수한 신생골 형성을 보였다. 비록 통계학적으로는 치아 회분말과 연석고 처치군과 치아 회분말과 연석고 및 알긴산 혼합 처치군이 유의한 차이를 보이지 않았지만 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 보다 더 우수한 신생골 형성 경향을 보였다. 뿐만 아니라, 신생골의 내용면에서도 치아 회분말과 연석고 및 알긴산 혼합 처치군에서 좀더 많은 신생 직조골의 융합이나 골 소주형성이 관찰되어 신생골의 형성 및 성숙에 알긴산이 역할을 담당하는 것으로 사료되었다. 결론적으로 골결손부 치료 방법으로 치아 회분말과 연석고 처치군 또는 치아 회분말과 연석고 및 알긴산 혼합 처치로 결손부에 이식하는 것은 신생골 형성의 양적, 질적 개선에 통계학적으로 유의하며, 알긴산을 이식재와 함께 처리한 경우에 신생골 형성의 양적, 질적 개선에 도움을 줄 수 있을 것으로 사료된다.$1^{\circ}C$ 냉수로 세척하고 PETE tray로 포장하여 $4^{\circ}C$로 저장한 경우 깻잎 고유의 초록색과 향을 유지하고 있어 저온냉수 세척과 tray 포장이 세척 청경채의 선도 유지에 효과가 있는 것으로 나타났다.1%,\;pendimethalin\;1.3{\sim}2.9%$ 및 $3.8{\sim}10.8%,\;ethoprophos\;0.6{\sim}2.7%$ 및 $0.1{\sim}0.3%$이었다. 인공강우실험 후 공약의 토심별 분포를 살펴 본 결과 alachlor와 ethopropho는 토심 $10{\sim}15cm$까지 이동하였고, ethalfluralin과 pendimethalin는 대부분 토심 5 cm 이내에 잔류하였다. 경사도 30%의 경우가 10%에 비하여 각 농약의 유실량이 $0.2{\sim}1.9$ 배 증가하였는데 유출수에 의한 농약의 유실량 차이는 유출수 중 농도 차이로 판단되며, 유실토양에 의한 농약 유실량 차이는 토양 유실량과 관계되는 것으로 생각되었다. 농약의 강우에 의한 유실은 복잡하게 작용하는 많은 환경적 요인에 의하여 영향을 받지만 정교하게 구성된 환경 시나리오에 의하여 예측 가능할 것으로 판단되었다.고 도라지는 물에 우려 푹 삶았고, 감자, 송이 등은 잘게 썰어 쌀과 함께 밥을 조리하였다. 4. 약선 음식조리방법 약선음식의 재료는 평상시 식생활에 사용되고 있던 식품들의 기능성분과 약이성을 이용하여 만성적인 질병과 급성적인 복통 설사 등에 재료의 전처리를 통해 죽으로 많이 이용하였다. 특히 곡류 등은

• Journal of Oriental Neuropsychiatry
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• v.10 no.1
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• pp.39-51
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• 1999

The effects of Hyangbujapalmultang on the enhancement of learning and memory of AD model rats were studied with Morris water maze. Sample group was electrolytically lesioned on nucleus basalis of Meynert(nbM), and then daily treated with the medicine for two months. Control group with nbM lesion, and sham group with the sham operation were treated the vehicle for the same duration. The following results were observed. 1. As the learning trials of Morris water maze were proceeded, sham group showed the escape latency from $54.7{\pm}2.28$ seconds in 1st trial to $13.3{\pm}3.27$ seconds in 7th. The control group showed the escape latency from $58.0{\pm}1.78$ seconds in 1st trial to $51.3{\pm}3.52$ seconds in 7th. The sample group showed the escape latency from $57.0{\pm}2.21$ seconds in 1st trial to $28.4{\pm}4.82$ seconds in 7th. Therefore, these data shows that all three groups were improved in learning capacity as trials were proceeded, but the sample group showed more prominent improvement in learning compared with the control group(p<0.05). 2. In memory retention test of Morris water maze that counts the staying time in the target area during 30 seconds of freely swimming period, sham group stayed for $4.81{\pm}1.15$ seconds, the control group stayed for $1.27\pm}0.78$ seconds, and the sample group stayed for $4.17{\pm}1.47$ seconds. The analysis of the memory retention data shows that the sample group marked more improvement in memory retention compared with the control group, but could not obtained statistically significant result(p<0.05). With the experimental results above, Hyangbujapalmultang can be supposed to have the improving effects on the learning of AD model rats induced by electrolytic lesion of nbM.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.1
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• pp.15-24
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• 2001

The purpose of this study is to evaluate the tissue response in applying of various bone substitutes included toothash-plaster mixture, resorbable hydroxylapatite (HA) and demineralized freeze-dried bone and to show the clinical usefulness of toothash-plaster mixture for the repair of craniomaxillofacial bone defect. For this experiment, 100 Sprague-Dawley rats weighing 200gm or more were used. There were four experimental groups: group I, toothash-plaster mixture; group II, demineralized freeze-dried bone; group III, resorbable HA; and group IV, control group. A full thickness, round bone defect measuring 10mm in diameter was created in the midcranium, and the substitutes cited above were embedded in the experimental rats based on their group assignment. Blood clot was filled in the rats assigned to the control group. Experimental rats were sacrificed on the 1st, 3rd, 5th, 8th, 12th and 24th week after implantation and stained with the hematoxylineosin, Masson's Trichrome, using Van Gieson's stain method, and were examined under light microscope. The results were as follows: 1. In all the groups, prominent inflammatory reaction and the infiltration of multinucleated giant cells were noted during the early stage. Gradual healing decreased this reaction. 2. Among the rats in the experimental group II, which were given demineralized freeze-dried bone implants, active formation of new bone traveculae manifested. Chondroid tissues appeared, and it was suggested that the defect was filled with newly formed bone by virtue of osteoinductive activity. On the 12th week after the experiments, most of the defect was filled with newly formed bone trabeculae. 3. In experimental groups I and III, it was noted that HA manifested a healing process similar to that characterized by the toothash-plaster mixture, but inflammatory reaction was more prominent in experimental group I. Active osteoblasts were observed along the periphery of osteoid tissues, while newly formed bone trabeculae appeared adjacent to the implanted materials three weeks later. Formation increased to the extent that newly formed bone trabeculae fused directly with the host bone. Increase in new bone ingrowth into the filling materials was revealed by both experimental groups. 4. In the control group, new bone formation adjacent to the host bone was observed, but most of the defect was filled with mature connective tissue 24 weeks after the experiments.