• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.94-98
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• 2013

A new species, Daphne jejudoensis M. Kim, has been named and described from Seonheul, Jejudo Province, Korea. Daphne jejudoensis shares several characteristics (white calyx colors, long calyx tube, evergreen leaves, leathery leaf textures, etc.) with its related species D. kiusiana, but it is distinct from D. kiusiana which has a hairy calyx tube and lobes, short calyx lobes, oblanceolate leaves (with acute apex), and a different distribution (coastal region in Geojedo Is.) by having a glabrous calyx tube and lobes, long calyx lobes, elliptic leaves (with acuminate apex), and by its distribution (inland region in Jejudo Is.).

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.246-250
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• 2010

This study was conducted to find out the propagation method of a rare variety Daphne kiusiana Miq. in Korea through softwood cuttings. Daphne kiusiana Miq are the evergreen broad leaf tree which have lived in the southern part of Korea. The flowering time of Daphne kiusiana Miq is early spring and the flower has an attractive fragrance. Generally, plants are dioecious and only the male plants are found in Korea. Propagation by seed is very difficult in this tree. Softwood cuttings were taken to establish an efficient propagation method in Daphne kiusiana Miq Cuttings were grown in perlite:vermiculite (1 : 1), perlite, vermiculite, sand rooting media under shaded greenhouses during summer in 2007. The rooting rates of cuttings were 86.7%, 95%, 75% and 95% in perlite:vermiculite (1 : 1), perlite, vermiculite and sand media, respectively. Softwood cuttings taken on July and rooting at $27^{\circ}C$ were significantly improved the growth and rooting rates. Softwood cutting can be an effective means of propagation in Daphne kiusiana Miq.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.61-61
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• 2018

한경 곶자왈 산림유전자원보호구역은 개가시나무와 백서향 등을 포함한 암괴지대의 상록활엽수림으로서 그 중요성이 인정되어 산림유전자원 생태보전지역으로 지정 관리되고 있다. 따라서 본 지역의 식물상을 조사하여 곶자왈의 분포식물을 파악하고자 실시하였다. 본 연구는 한라산 서쪽 곶자왈지역 중 곶자왈 산림유전자원 보호구역으로 지정된 지역을 2017년 6월부터 2017년 9월까지 20여 차례 현장조사를 통하여 표본을 수집하고 이를 기록 정리하였다. 조사지역의 식물은 총 83과 181속 236종 23변종 2품종 총 261종으로 조사결과 양치식물이 7과 18속 30종 2변종 32분류군, 나자식물이 1과 1속 2분류군, 피자식물은 74과 162속 204종 21변종 2품종 227분류군 이었다. 그 중 쌍자엽식물은 67과 139속 179종 16변종 1품종 196분류군이고, 단자엽식물이 7과 23속 25종 5변종 1품종 31분류군이었다. 조사에서 확인된 환경부 멸종위기야생식물은 개가시나무 1종 이었다. 식물 구계학적 특정식물로는 총 97분류군이 조사되었다. 등급별로는 V등급 검정개관중, 개가시나무로 2분류군, IV등급 백서향, 녹나무 등 12분류군, III등급 아왜나무, 호자나무 등 37분류군, II등급 올벚나무, 개산초 등 13분류군, I 등급 푸조나무, 자금우 등 33분류군으로 확인되었다. 한국의 적색목록 식물을 분석해보면 취약(VU)은 개가시나무, 섬다래 등 3분류군, 준위협(NT)은 백서향, 약난초로 2분류군, 관심대상종(LC)은 골고사리, 새우난초 등 9분류군, 미평가종(NE)은 빌레나무 1분류군으로 나타났다. 이 결과는 청수 저지 곶자왈 지역의 생태학적 자료로 제주도 내 곶자왈과 비교하였을 때 맹아가 발달한 종가시나무가 우점하는 지역으로 향후 상록수림 천이에 의해 상록수 아래에 자라는 식물로 낙엽활엽수가 대체될 것으로 추정되어 지속적인 조사가 요구된다.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.1
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• pp.41-51
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• 2007

목적 : OVA에 유도된 천식 쥐 모델에 향소산가미방을 투여한 후 $PPAR{\gamma}$의 변화를 조사하여 향소산가미방의 천식 치료 기전을 알아보고자 하였다. 대상 및 방법 : 8주된 암컷 BALB/c 마우스에 첫 날과 14일 후에 20 ${\mu}g$의 OVA를 알루미니윰 하이드록사이드 1mg과 혼합한 후 총 200 ${\mu}g$를 복강 내로 주입하여 감작시켰다. 처음 감작시킨 날로부터 21,22,23일 후에 천식 모델에 사용하는 초음파 분무기를 이용하여 세 번째 감작시켜서 천식 마우스 모델을 만들었다. 천식 마우스 모델을 만드는 기간 중 OVA를 복강 내로 주입한 후 19일째에 24시간의 간격으로 향소산가미방을 7일 동안 경구 투여하여 향소산가미방의 효과를 조사하였다. 기관지폐포세척술은 마지막 감작 후 72시간 후에 실행하고 기관지폐포 세척액의 총 세포수를 측정하였다. $PPAR{\gamma}$의 발현은 천식 마우스 모델의 폐와 향소산가미방을 투여한 마우스 모델의 폐를 적출한 후 Western blotting 방법을 아용하여 측정하였다. 병리 조직학적 검사는 hematoxylin 2 and eosin-Y 염색을 이용하여 조사하였다. 결과 : 정상 군과 비교하여 OVA감작 천식 쥐 모델에서는 72시간 후에 총 세포 수가 증가하였다. 특히 OVA감작 천식 군에서 증가된 호산구의 수가 향소산가미방을 투여 한 쥐 군에서는 유의하게 감소하였다. OVA감작 천식 쥐 모델에서 72시간 후에 정상 군과 비교하여 핵 내에서의 $PPAR{\gamma}$단백질의 발현이 약간 증가하였다. 그러나 향소산가미방을 투여한 쥐 모델에서는 세포질과 핵 내에서 $PPAR{\gamma}$단백질의 발현이 유의하게 증가하였다. 조직학적 검사상 정상 군과 비교하여 OVA 감작된 천식 쥐 모델에서는 폐포, 세기관지, 기도내강 주변에 많은 염증 세포들이 있었다. 그러나 가미향소산 을 투여 한 후에는 염증 세포들이 유의하게 감소하였다. 결론 : 가미향소산은 $PPAR{\gamma}$작용제로서 역할을 하며, 천식에 대한 치료제 또는 예방제를 개발하는 데 후보 물질이 될 것으로 사료된다.

• Journal of Life Science
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• v.12 no.6
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• pp.649-653
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• 2002

The vegetation of Seoimal-lighthouse area was investigated from August, 2001 to July, 2002. In order to analyze the vegetation of this area, synthesis table, actual vegetation map were prepared. The main community structures of this area represent evergreen broad-leaf community(about 48%), which are very good condition(nearly last sere) to preserve and 11communities(Camellia japonica community, Castanopsis cuspidnta var. thunbergii community, Neolitsea sericea community, Quercus serrata community, Quercus variabilis community, Carpinus corenna community, Srtrax japonica community, Pinus thunbergii community, Quercus acutissima community, Zelkova serrata community, Cinnamimum japonicum community) were classified by vegetation type. And Daphne kiusiana, Asirum maculatum, Cymbidium goeringii, Chloranthus glaber, Ligularia taguetii should be worthwhile species to conserve in this area.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.23-23
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• 2019

There are many island areas in Jeonnam Province and various plants are naturally grown due to their regional characteristics. Among them 217species were found to be Rare-Specialty plants in Jonnam Province. There are growing 35 species of Critically Endangered(CR), 37 species of Endangered Species(EN), 54 species of Vulnerable(VU) and 36 species of Least Concern(LC), many of which are native to islands far south or western of the sea. This report briefly describes the geographical distribution location and the state of reproduction among Rare-Specialty woody plants grown in the Warm-temperature Zone of Jeonnam Province. There are growing Michella compressa(Shinan-gun), Actinidia rufa(Wando-gun), Glochidion chodoense(Jindo-gun), Rhus ambigua(Yeosu), Wikstroemia ganpi (Goheung-gun, Yeosu) among of the 35 Critically Endangered species. Woody plants of Endangered Species(EN) are growing Albizia kalkora(Mokpo, Muan-gun), Daphne kiusiana(Shinan-gun), Marsdenia tomentosa(Wando-gun), Osmanthus insuralis(Yeosu, Shinan-gun). Woody plants of Vulnerable(VU) are growing Ardisia crenata(Wando-gun, Shinan-gun), Hibiscus hamabo(Wando-gun), Illicium anisatum (Jindo-gun), Koelreuteria paniculata(Wando-gun), Wikstroemia ganpi(Youngam-gun). Woody plants of Least Concern(LC) are Celtis choseniana(Yeosu), Chionanthus retusus(Wando-gun, Goheung-gun), Cinnamomum camphora(Wando-gun, Jindo-gun), Linder sericea(Boseong-gun).

• The Journal of Pediatrics of Korean Medicine
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• v.25 no.1
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• pp.40-48
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• 2011

Objectives: The purpose of this study is to evaluate the effect of Gonjadaesungchimjoongbang on spatial learning abilities and memories in ischemic brain injury. Methods: Rats were separated into three groups; (1) Normal, (2) Saline medication after ischemic brain injuries (control), (3) Gonjadaesungchimjoongbang medication after ischemic brain injuries (experiment). Ischemic brain injuries was induced by MCA occlusion and reperfusion. Morris water maze test was conducted for spatial learning and memory tests. Then, the change of BDNF in the hippocampus($7^{th}$, $14^{th}$ day) was examined by immunohistoche- mistry. Results: In Morris water maze test, spatial learning abilities and memory functioning were considerably increased in the experiment group as oppose to control group on $7^{th}$ and $14^{th}$ day(p<0.01). Moreover, immunohistochemistric response of BDNF in the hippocampus indicated that the more increased immune reaction was found in the experiment group as oppose to the control group on $7^{th}$ and $14^{th}$ day. Conclusions: Gonjadaesungchimjoongbang can improve the learning abilities and memories in ischemic brain injury.

• The Journal of Korean Obstetrics and Gynecology
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• v.23 no.4
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• pp.35-46
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• 2010

Purpose: This study was designed to investigate the effects of Cyperi Rhizoma (CR) on changes in weights morphological and histopathological observations in Polycystic Ovary(PCO) rats. Methods: PCO was induced by single intramuscular injection with EV(2mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for five weeks. PCO control group(n=8) were injected with EV and orally administrated distilled water for five weeks. CR treated group(n=8) were injected with EV and orally administrated CR for five weeks. Then we measured weight of body, ovaries. The histopathology changes of ovaries, and the expression of nerve growth factor(NGF) were also evaluated. Results: Single injection of EV induced suppression of weight gain, formation of cysts, increase of NGF expression. Oral administration of CR prevent suppression of weight gain shown in control group. In addition CR group showed upward tendency of ovarian size. Formation of cystic follicles induced by EV injection is suppressed by CR treatment. In additon, CR treatment lowered expression levels of nerve growth factor(NGF), which were elevated by induction of PCOS. Conclusion: These results suggest that CR can be used for patients with PCOS to prevent formation of cystic follicles and malfunction of ovary. And also suggest that the related mechanisms are involved in suppression of NGF expression.

• The korean journal of orthodontics
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• v.33 no.2 s.97
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• pp.103-111
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• 2003

The Purpose of this study was to evaluate the effect of occlusion on the mechanical strength of periodontal fibers during retention periods after experimental tooth movement. In the Sprague-Dawley male rats weighing 200g or more, the ntraoral elastics were inserted into the both right and left interproximal space between upper first and second molars for tooth movement. kiter 4 days later, the left lower first, second, and third molars were extracted for differentiating the non-occlusal side from the occlusal side in the same mouth. At the same time the elastics were removed and then light cured resin was Placed in the space between upper first and second molars following undercut was made for retention bilaterally. From the beginning of retention, 7 rats were sacrificed at 0, 4, 8, 12, 16, 20 days respectively. For evaluating of magnitude on the mechanical strength of periodontal tissue, the maximal shear load of the upper first molars were measured bilateraly during extraction using Instron Universal Testing Machine. The results of this study were obtained as follows : 1. In the occlusal side, the maximal shear load was increased from no retention to retention 20 days group as time was going and statistically difference was shown from retention 12 days group (p<0.05). 2. In the non-occlusal side, the maximal shear load was increased slightly from no retention to 20 days group as time was going but there was no statistically difference (p>0.05). 3. The result compared with the maximal shear load between occlusal and nonocclusal side showed no statistically difference until retention 8 day group (p>0.05), but showed statistically difference from retention 12 day to 20day group (P<0.05). These results show that occlusion had an effect on mechanical strength of the periodontal fibers during retention periods after experimental tooth movement; therefore, it is suggested that occlusion should be considered while the retainer types and retention period are planned.

• Tuberculosis and Respiratory Diseases
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• v.49 no.1
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• pp.37-48
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• 2000

Backgrounds : The pathophysiology of chronic airflow obstruction, such as bronchial asthma, is characterized by mucus hypersecretion, goblet cell hyperplasia(GCH), smooth muscle hypertrophy, and inflammatory cells infiltration. In fatal asthma patients, one distinct findings is mucus hypersecretion due to GCH. However, the mechanisms of GCH in these hypersecretory diseases remain still unknown. In this study, a rat model was rapidly induced with GCH by instillation of $TiO_2$, intratracheally. We intend to confirm GCH and association of concomitant inflammatory cells infiltration and to observe the effect of potent antiinflammatory agent, that is dexamethasone, on GCH with inflammatory cells. Methods : Twenty-one 8-weeks-old male Sprague-Dawley rats were divided into three groups. Endotoxinfree water was instilled intratracheally in group 1(control) ; $TiO_2$, was instilled in the group 2 ; and dexamethasone was injected intraperitoneally to group 3 before $TiO_2$ instillation. After 120 hours, all rats were sacrificed, and trachea, bronchi, and lungs were resected respectively. These tissues were made as paraffin blocks and stained as PAS for goblet cells and Luna stain for eosinophils. We calculated the ratio of goblet cell to respiratory epithelium and number of infiltrated eosinophils from each tissue. Results : (1) Fraction of goblet cells was significantly increased in group 2 than in group 1 in the trachea and in the main bronchus. (10.19$\pm$11.33% vs 4.09$\pm$8.28%, p<0.01 and 34.09$\pm$23.91% vs 3.61$\pm$4.84%, p<0.01, respectively). (2) Eosinophils were significantly increased in the airway of group 2 than that of group 1. (5.43$\pm$3.84% vs 0.17$\pm$0.47 in trachea and 47.71$\pm$16.91 vs 2.71$\pm$1.96 in main bronchi). (3) There was a positive correlation between goblet cells and eosinophils(r=0.719, p=0.001). (4) There was significant difference in the decrease of goblet cells after dexamethasone injection between group 2 and group 3 (p<0.01). Also, infiltration of eosinophils was suppressed by dexamethasone. Conclusion : We made an animal model of $TiO_2$-induced goblet cell hyperplasia. GCH was observed mainly in the main bronchi with concomitant eosinophilic infiltration. Both goblet cell hyperplasia and eosinophilic infiltration were suppressed by dexamethasone. This animal model may serve as a useful tool in understanding of the mechanism of GCH in chronic airway diseases.