• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.327-334
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• 2006

In tomato perlite bag culture, the available water content was analyzed for several sorts of perlite so that the irrigation safety was investigated and thus the irrigation strategy controlled by time clock was established. The reduction trends of water content in perlite bags were monitored for tomatoes, of which fruits were harvested until the fifth cluster. The amount of daily reduction of water in the bag was on the decrease as the total water in the bag was decreased. In terms of time interval from when the water content based on weight was reduced more than 50g to when it was dropped again, the longest time interval in a day was retarded gradually. It means plant activity was recovered later than the previous day. The available water content in perlite bag of 40 liters was about 30% which was 12 kg in weight, which satisfied daily water demand of 6 tomato plants. The appropriate time irrigated by time clock was recommended for the case that it was irrigated 5 or 10 times a day with the daily integrated solar radiation of 601 or $1,519W/m^2$.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.273-282
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• 2008

The relationship between water deficit stress and monodehydroascorbate reductase (MDHAR) activity was determined in lettuce (Lactuca sativa L.) leaves under water stress condition imposed by with-holding water for 72 hrs. Relative water content determined in water deficit stressed lettuce leaves gradually reduced from 91.29% to 74.58%, and water content of medium drastically decreased 4.73% after quitting of irrigation. Hydrogen peroxide content in leaves subjected to water deficit stress rapidly increased, but soluble protein content rapidly decreased when those were compared to control plant. The relationship between relative water content and hydrogen peroxide content in stressed leaves positively correlated with $R^2$=0.8851, but soluble protein content reversely correlated with $R^2$=0.9826. Total chlorophyll content in stressed plant leaves was higher than that of control plant, and increased rapidly in early stage of treatment of both stressed and control plants. Carotenoid content was higher than that of control plant, and the ratio of carotenoid to total chlorophyll in stressed plant was higher as compared to control plant. As water deficit stress continued progressively, total ascorbate content in stressed plant leaves was a little higher than that of control plant. But dehydroascorbate (DHA) content within 6 hr of water deficit stress was higher than that of control plant, and then, content of control plant in 12 hr of stress treatment higher than that of stressed leaves. The activity of monodehydroascorbate reductase of cytosolic and chloroplastic tractions increased dramatically, and mRNA of MDHAR was highly detected by probing $^{32}P$-labeled single stranded MDHAR RNA of lettuce plant leaves subjected to water deficit stress. Relationship between MDHAR activity and relative water content and hydrogen peroxide highly correlated with $R^2$=0.9937 and 0.8645, respectively.

• Journal of Mushroom
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• v.13 no.3
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• pp.207-211
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• 2015

To investigate the usefulness of Kenaf(Hibiscus cannabinus L.) as mushroom culture media source, we analyze physical condition and contents of nutritional components. The water absorption rate of Kenaf bast was 578% and it was 95% higher than that of poplar sawdust's. This was caused by Kenaf's porous cellular structure. so it could give more moisture and oxygen to cultured mushroom. Total carbon contents of Kenaf was 91.4%, it was quite higher than that of poplar sawdust, wheat bran and rice bran. Total nitrogen content was 1.76% and C/N ratio was 51.9. The content of NFE(Nitrogen free extract) was 46.6% and it was similar with rice bran. Cellulose content was higher than poplar but lignin content was lower. specially hemicellulose and pectin complex which more digestible carbon source to mushroom was 3.7% higher than poplar. Mineral component and amino acid contents were also maintained high compared with poplar. Fe was 4.2 times, P 3.2 times, K 2.2 times more and Ca was higher 16 mg/kg than poplar. The content of amino acid was quite more higher than poplar sawdust but lower than chaff. Consequently Kenaf had a good trait for basic support material in mushroom culture media and also had a good character as nutritional source.

• Journal of Mushroom
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• v.14 no.1
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• pp.14-20
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• 2016

This study was conducted to establish replacement the corncob used in winter mushroom bottle cultivation. Corncob is unstable quality in moisture content or total nitrogen(T-N) content. Fruit body yields according to the ratio of cassava stem chips mixing were compared. After treatment-1 and treatment-2, fruit body yields increased by 8.8% and 5.4% and raw material cost decreased by 7% and 19%. The results showed that cassava stem chips could replace 33% to 67% of corncob for winter mushroom bottle cultivation.

• Journal of Mushroom
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• v.10 no.4
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• pp.174-178
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• 2012

This study were carried out to analyze nutritional ingredients of pre-inoculation and post-harvest Flammulina velutipes media. pH, moisture content, total carbon(T-C), total nitrogen(T-N), crude protein, crude fiber, and crude fat content on the 8 raw materials using Flammulina velutipes bottle cultivation medium, and after sterilization(pre-inoculation) and post-harvest medium was analyzed. This result is expected to be utilized as the basis for recycling post-harvest media.

• The Korean Journal of Mycology
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• v.4 no.1
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• pp.31-44
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• 1976

The studies were carried out to examine the effects of supplementation of nutritional substances and physical conditions in substrate on the mycelial growth and yield of fresh sporophores of winter mushroom, Flammulina velutipes(Curt. ex Fr.) Sing. and to obtain further informations on the nutritional requirements of the fungus with reference to improvement of substrate through [analysis of chemical composition of the substrates during the cultivation period. The results obtained are summarized as follows: 1. The best yield of fresh sporophores, 84.4 g per 280 g substrate in a bottle, was obtained from the mixture of poplar sawdust 10 and rice bran 3 by volume when Flammulina velutipes was cultivated on the poplar sawdust supplemented by rice bran, wheat bran, cattle manure and various combinations of these materials as nutrient sources. The substrates of poplar sawdust 10 plus rice bran 3 and 2 or wheat bran 3 with a higher yield of fresh sporophores showed a comparatively higher content of total nitrogen. total sugar, and potassium. 2. The mycelial growth of the fungus was compared on the substrates of poplar sawdust supplemented by the several nutrient sources and poplar sawdust alone. The fastest linear growth occurred on substrates of poplar sawdust alone and poplar sawdust plus cattle manure deficient in sugar and nitrogen sources, but mycelial density was more sparse on the substrates. Also, growth in a solution extracted from these substrates was very meager. 3. In the substrates which varied with bulk density and moisture content optimum bulk density and moisture content for mycelial growth was 0.2g/cc and 72% on a dry weight basis, respectively, but the highest yield of fresh sporophores was obtained at the bulk density of 0.3g/cc and moisture content of 67%. 4. By increasing the ratio of rice bran in poplar sawdust the loss of total weight and ash, content at each stage was increased, and during the cultivation period of 75 days, loss of total weight of the substrates at inoculation was 17.8 to 28.8% and ash content increased about 12%. 5. 11 to 14% of the cellulose and 3 to 4% of the lignin content per original substrate were decreased without a great difference depending of the mixing ratio of rice bran. The soluble glucose concentration in the substrates was increased during the same period. 6. In the process of vegetative and reproductive growth of the fungus upon the substrates, the total nitrogen was increased in quantity per dry weight of sample but was reduced in absolute quantity to a minute extent. There is no great changes in content of organic nitrogen including amino acid nitrogen, and hydrolysable ammonium nitrogen during the vegetative growth period, but occurrence of sporophores resulted in a decrease in the nitrogen content of these forms. On the one hand, by an increase of additive amounts of rice bran, nitrogen contents of these forms were higher and the reduction range during the reproductive growth period became wider. 7. Mycelial growth of the fungus was accelerated in various liquid media supplemented with organic nitrogen sources such as peptone and yeast extract in comparison with addition of inorganic nitrogen sources. Furthermore, mycelial growth was mere vigorous in the media with higher content of organic nitrogen sources.

• Journal of Mushroom
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• v.6 no.3_4
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• pp.131-137
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• 2008

These experiments were conducted to search for the optimum media size and composition for culture of Lentinula edodes (JNM3022). Considering the incubation period and the successful culturing rate, the optimum media size was length 25 $cm{\times}diameter$ 14 cm (weight 2.5 kg). The saturation moisture amount of Oak sawdust was 251 ml/100 g. It is a half of pine sawdust 495 ml/100 g. Considering the hypha growing speed and hypha density, the optimum media composition was Oak sawdust 80%+ wheat bran 20% and Oak sawdust 80%+ rice bran 20% In 10 treatments of media composition. The optimum moisture amount of media for growing Lentinula edodes (JNM3022) was 55%. Injection pipe with 20 holes in media was reduced the incubation periods to 11 days when 20cm length media and 32 days when 30cm length media compare to control. The best method for browning media surface was change temperature condition above $10^{\circ}C$ and punching the vinyl bag. This treat could shortened the browning periods to 54 days.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.316-325
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• 2009

Adequate conditions of water content and aeration of container media are major environmental factors in the production of pot plant. This experiment was carried out to find optimum physical property of media for the production of small potted Ardisia in capillary mat irrigation system. The plant materials used in this experiment were Ardisia pusilla and Ardisia japonica. Seven substrates were formulated by blending perlite or fresh rice hulls at 20%, 40%, 60% (v/v) with sphagnum peat. Total pore space (TPS) increased by blending sphagnum peat with fresh rice hulls, but decreased by blending sphagnum peat with perlite. As fresh rice hull (FRH) and perlite content increased, air filled pore space (AFP) of substrate increased but container capacity (CC) decreased. Substrate blended with fresh rice hull was higher AFP than blended with perlite and the rate of increase was higher for FRH-containing substrate. As AFP increased, the $CO_2$ concentration in the pot decreased and the $CO_2$ concentration of substrate blended with FRH was higher than blended with perlite. The fresh and dry weight of Ardisia pusilla and A. japonica was the highest in the substrate contained 60% FRH, but the ratio of shoot dry weight to root dry weight was the lowest. The optimum total pore space, air-filled pore space, water holding capacity of substrate for the growth of Ardisia pusilla and A. japonica in the capillary mat irrigation system were 82.8%, 25.6%, and 57.2% respectively.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.150-154
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• 2016

To determine a favorable substrate formulation for Grifola frondosa, physicochemical conditions, culture properties, and yields according to various substrate formulations were investigated. Based on these analyses, T4 (80:5:15 ratio of oak sawdust to dried bean-curd refuse to corn husk) resulted in a shorter cultivation period and higher yields (weight of fresh mushrooms harvested at maturity) than those of other treatments. The physicochemical properties of T4 were pH 5.4, 2.4% crude fat contents, 54 C/N ratio, 74.3% porosity, and 0.26 g/cm3 bulk density. These results emphasize the importance of optimal substrate development on the production efficiency of G. frondosa mushrooms and have implications for commercial applications.

• Journal of Mushroom
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• v.10 no.3
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• pp.136-142
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• 2012

This study was carried out to determine the supplemental level of spent mushroom (Flammulina velutipes) substrates as an energy source in manufacturing of high moisture sorghum whole crop silage. Whole crop sorghum was harvested at heading stage and ensiled with spent mushroom substrates of 20% (S-20), 40% (S-40) and 60% (S-60) as fresh matter basis. Each silage was manufactured in plastic buckets included vinyl bag by three replications and stored for 0, 3, 6 and 9 weeks, respectively. Fermentation characteristics and quality of sorghum silages manufactured by supplemental level of spent mushroom substrates were as follows. Moisture contents of whole crop sorghum and spent mushroom substrates were 83.85% and 54.3%, respectively, and that of silages was 78% for S-20, 71% for S-40 and 68% for S-60. Ether extracts content of silages was significantly (P<0.05) increased during the fermentation periods. The pH in silages fermented for 3 weeks and above ranged from 4.24 to 4.42, and the decrease of pH by fermentation was relatively greater in S-40 compare to the other treatments. The lactic acid content of silage inclined that the S-40 was higher compared to the other treatments and decreased with elongation of fermentation period of silage. The contents of acetic acid and propionic acid of silages were not influenced by treatments and fermentation period. Flieg's score for estimation of silage quality ranged from 60 to 83, and was relatively high quality in the S-40 fermented for 9 week, and was relatively low quality in the S-60 fermented for 9 week. From above results, we suggest that 40% supplementation of spent Flammulina velutipes mushroom substrates as an energy source is resonable level in manufacturing of high moisture sorghum whole crop silage.