• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.43-43
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• 2002

체세포 핵이식에 의한 산자가 양, 소, 염소, 쥐 및 최근에 돼지에서 보고되었지만, 생산된 배반포의 동결보존에 관한 연구결과는 보고되지 않고 있다. 따라서, 본 연구는 체세포 핵이식란의 활용과 산업화 촉진을 위해서 체세포 핵이식 유래 배반포의 동결성 향상에 배양조건이 미치는 영향을 조사하고자 실시하였다. 공여세포는 60일령의 태아세포를 10% FBS 가 첨가된 DMEM 에서 39℃, 5% CO₂의 incubator에서 배양하여 monolayer confluent 형성을 유도한 후 0.25%trypsin 처리하였다. (중략)

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.254-255
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• 2000

형질전환된 인삼 모상근의 여러 생물반응기에서의 대량배양 가능성을 알아보기 위하여 1/2 MS 배지를 기본배지(호르몬 무첨가, 3% sucrose)로 하여 실험을 수행하였다. 그 결과 플라스크 배양에 비하여 $2{\sim}3.5$배 높은 성장률을 보였으며, 그 성장이 왕성함을 알 수 있었다. 배양 후반기에 모상근의 뭉치 형성으로 물질전달의 어려움으로 인하여 성장에 장해를 미치는 것으로 사려된다.

• KSBB Journal
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• v.16 no.6
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• pp.568-572
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• 2001

Light is one of the most important environmental factors controlling plant physiology. The human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was produced from cell suspension cultures of transgenic tobacco under different light conditions (24 hr light, 18 hr light/dark cycle, dark). Under 24 hr light condition, cell growth was best and dry cell weight reached 14.4 g/L. Light did not influenced the secretion of total proteins. However, in the dark condition, the ratio of secreted total protein/dry cell weight was 1.5 fold higher than those of ethel conditions. Production of hGM-CSF was highest with 18 hr light condition and reached 496.5 ug/L. In addition, the content of hGM-CSf in secreted total proteins was 1.8 fold higher than that of 24 hr light condition, which is beneficial for the purificationof the protein.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.369-374
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• 1997

Immature flower buds of 'Desio' carnation were cultured on MS agar medium supplemented with 1 ㎎/L 2,L-D. Embryogenic calli were formed from 5-10% of the buds less than 20 ㎜ in length, but only non-embryogenic calli were produced from explants of shoot apex leaf, internode, and flowere buds larger than 20 ㎜. The same method was applied to 16 cultivars of cut Sower carnation and embryogenic calli were obtained in 7 cultivars. Several embryogenic callus lines were selected and maintained through subcultures over 120 weeks without loss of embryogenic competence. The embryogenic cultures were also proliferated rapidly in liquid agitation cultures using MS medium supplemented with 1mg/L 2,4-D. Numerous embryos were formed on the periphery of the cell aggregates upon transfer to auxin-free MS agar medium. Plantlets were transplanted in potting soil and grown to bloom in six months.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.81-86
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• 2008

This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.

• Korean Journal of Microbiology
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• v.33 no.1
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• pp.43-48
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• 1997

여러가지 난분해성 물질에 대한 생분해능을 지닌 백색부후균에 의한 매립지 침출수의 탈색을 조사하였다. 국내에서 분리한 Coriolus versicolor KR-11W와 Irpex lacteus KR-39W가 이제까지 주로 연구되어 온 Phanerochaete chrysosporium보다 높은 탈색능을 나타내었는데 I. lacteus KR-39W는 산소공급시 10%의 침출수가 함유된 YMG 배지의 진탕배양에서 85%의 색도제거율을 나타내었으며 최소배지에서도 80%의 탈색율을 보였다. P. chrysosporium에 의한 리그닌 분해능 및 분해효소 생성 보고들과 달리 진탕배양이 정치배양보다 탈색능이 높았으며 산소공급은 색도제거에 증가효과가 있었다. 균체 접종량(10-30%)과 온도(25,37.deg. C)는 탈색에 큰 차이를 보이지 않았으나 탄소원과 질소원의 농도는 상당한 영향을 나타내었다. 리그닌 분해효소군의 여러 가지 inducer와 cofactor를 C. versicolor KR-11W 배양에 첨가한 결과 많은 경우 균접종 대조군보다 2배 이상의 탈색율과 lignin peroxidase 활성의 증가를 보였으며 FeS $O_{5}$ 첨가시에는 최대 2.9배의 증가를 나타내었다. 탈색에 관여하는 효소군은 접종물에 이미 어느 정도 존재할 수 있으며 배지 및 배양조건에 따라 그 생성이 변화할 수 있으므로 이런 조건들을 잘 맞출 경우 보다 높은 탈색능이 기대된다.

• Korean Journal of Animal Reproduction
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• v.26 no.1
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• pp.41-51
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• 2002

The present study was carried out to examine the effect of cysteamine in vitro maturation (IVM) of porcine oocytes and development of porcine IVM/IVF Embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, pronuclear formation, polyspermic oocytes and mean numbers of the penetrated sperm were not different in NCSU23 maturation medium with 0, 25, 50 and 100 $\mu$M cysteamine (P〉0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization in 0, 25, 50 and 100 $\mu$M cysteamine were 17.9$\pm$6.1, 17.4$\pm$6.3, 24.2$\pm$1.9 and 16.9$\pm$2.0%, respectively. And the total cells were 30.7$\pm$2.4, 34.9$\pm$2.8, 39.6$\pm$2.3 and 36.8$\pm$3.6, respectively. Fifty $\mu$M cystealnine group was significantly higher than those of any other treatment groups (P＜0.05). 3. The ratios of ICM/total cells in 20~40% category were 20.5, 41.6, 19.5 and 31.5%, respectively. Twenty five $\mu$M cysteamine group was higher than those of other groups. 4. The rates of blastocyst formation at day 7 in the NCSU-23 culture medium of porcine IVF-produced embryos with 0, 25, 50, and 100 $\mu$M cysteamine were 16.0$\pm$0.2, 13.6$\pm$1.7, 25.0$\pm$0.8 and 15.7$\pm$4.5%, respectively. And the total cells were 27.0$\pm$3.7, 36.1$\pm$4.8, 34.0$\pm$3.8 and 25.2$\pm$4.4, respectively. Fifty $\mu$M cysteamine group was significantly higher than those of any other treatment groups (P＜0.05). 5. The ratios of ICM/total cells in 20~40% category were 53.8, 30.0, 16.6 and 11.1%, respectively. The addition groups of cysteamine were lower than those of control group. In conclusion, these results suggested that the addition of 50 $\mu$M cysteamine in the IVM medium and 25~50 $\mu$M cysteamine in IVC medium were effective on the blastocyst formation and total cells of blastocysts.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.345-349
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• 1997

Somatic embryos were induced through embryogenic callus derived from immature zygotic embryo culture of native Prunus yedoensis in Mt. Halla and regenerated into plantlets successfully. Embryogenic callus was induced most effectively on MS medium with 1.0 mg/L 2, 4-D and 0.1 mg/L BAP at an efficiency of approximately 60% using 45 day-old zygotic embryos after full blooming. Globular somatic embryos were induced from embryogenic callus on MS medium with 1.0 mg/L 2, 4-D and 0.1 mg/L BAP and these globular embryos developed to heart-shaped and cotyledonary embryos on hormone-free MS medium. Normal somatic embryos germinated 49% on 1/2 MS medium and the plants regenerated from the somatic embryos were morphologically normal.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.241-244
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• 1999

The effect of Milliwrap and Uniwrap attached to the lid on the growth and acclimation of lily growing in vitro was investigated. Milliwrap treatment increased the plant height 1.6 and 1.2 times higher than control in 'Dame Blanche' and '94-36', respectively, plant height of 'Dame Blanche' was 6.1㎝ and '94-36' line was 13.4 ㎝. Two treatments slightly inhibited the fresh weight in 'Dame Blanche', whereas Milliwrap and Uniwrap enhenced the fresh weight more than 30% in '94-36'. Leaf width (1.0 ㎝) of Milliwrap treatment was most wide, which was 2.5 times higher than that of control. In the fresh weight of bulblets, the fresh weight per bulblet of treatment was higher than control in '94-36', but the number of bulblets per scale were few in the treatment to control. In the air composition of culture vessel, ethylene content (0.03 ppm) was low in the Milliwrap treatment compared with the control. CO₂ content of control was higher than treatments as 0.11% and it was about 3 times to air condition of out side. The acclimation ratio of Milliwrap treatment was higher as 85.1% and control was similar with Uniwrap treatment.

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.71-76
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• 1999

Anthers of two hot pepper cultivars, Milyang-jare and Geryongsan-jare, were cultured on MS medium containing 0.1 mg/L 2,4-D and 0.1 mg/L kinetin. The influence of pretreatment at 4$^{\circ}C$ and 32$^{\circ}C$ on induction of microspore embryo was investigated. Milyang-jare was superior to the Geryongsan-jare in microspore embryo induction. The 32$^{\circ}C$ pretreatment increased embryo induction compared to the 4$^{\circ}C$ pretreatment while the 4$^{\circ}C$ pretreatment stimulated callus induction. Microspore embryos were regenerated to plantlets in the same medium or hormone free medium at 32$^{\circ}C$ treatment but most embryos failed to develop directly into plantlets at 4$^{\circ}C$ treatment. The optimal period of the 32$^{\circ}C$ pretreatment was 3 days in Milyang-jare and 6 days in Geryongsan-jare. The 32$^{\circ}C$ pretreatment was essential for induction and growth of microspore embryo in pepper.