• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.11a
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• pp.174-175
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• 2001

미나리 묘를 대량으로 생산하는데 있어서 체세포배를 이용하는 방법이 매우 유리하다. 체세포배는 계절에 영향을 받지 않고 좁은 공간에서 한꺼번에 많은 량의 묘를 생산할 수 있다. 또한 생물반응기 등을 이용하여 대량 생산할 수 있으면 실생묘를 대체할 수 있을 것으로 생각된다. 배양기 내의 공기 환경의 영향은 삼각플라스크를 이용한 소규모 배양에서는 문제가 되지 않았지만 배양기가 커짐에 따라 공기 환경의 변화도 커져 체세포배의 발생과 발아에 영향을 미친다. 체세포배를 배양하는 기간은 배발생과 발아로 나누어, 세포에서 배가 발생하는 처음 2주 동안에는 밀폐 환경, 즉 환기가 거의 되지 않는 환경이 유리하지만 이후의 발아 동안에는 산소를 많이 요구한다. 삼각플라스크를 이용한 소규모 배양에서는 aluminum foil을 플라스크의 뚜껑으로 하여, 배지를 교체할 때를 제외하고는 배발생과 발아가 진행되는 전 배양 기간 동안 이를 유지한다. 배가 발생하는 초기 2주의 경우는 이 밀폐 환경이 발아를 촉진시킨다. 그러나 배가 발아하여 정상 식물체로 발달하는데는 유리하지 않다. 체세포배는 발아할 때 산소를 많이 필요로 하며 에틸렌이 많이 축적되면 발아율이 낮아지며, 적절한 공기 환경이 주어지면 체세포배 발아가 동조화되어 균일한 묘를 얻을 수 있고 수확시기도 예측할 수 있다. 본 실험에서는 배가 발아하는 기간 동안 플라스크 내의 공기 환경을 다르게 하기 위해 플라스크를 막는 뚜껑의 소재를 달리하여 배양한 후 발아율을 측정하였다. 또한 가장 효율적인 환기량을 구명하기 위하여 인위적으로 플라스크에 공기를 넣어 강제 환기시키는 실험을 수행하였다. 미나리 cell은 처음 8일까지는 생장을 하지 않다가 이후 급속히 생장을 시작하여 35일 정도까지는 생장을 하다가 다시 생장이 둔화되었다. 밀폐시킨 삼각플라스크에서 자라는 Cell은 상태도 좋지 않고 전반적인 증식량도 적었다. Cell은 환기정도에 민감한 것으로 판단되며 삼각플라스크에서 약 35일 정도의 생장 주기를 가지는 것으로 사료된다. 배양 3주까지는 플라스틱 뚜껑으로 밀폐시킨 bottle에서 가장 많은 체세포배를 얻었다. Air filter를 달아 2일 마다 신선한 공기를 넣어 주었을 때는 배의 발달이 많이 늦어져 배양 3주째에 다른 처리보다 배의 수가 훨씬 적었다. 체세포배가 발달하는 동안에는 산소를 많이 요구하지 않으나 성숙하는 동안에는 산소를 많이 요구하는 것으로 생각된다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.465-467
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• 2007

애기장대에 납을 농도별로 처리하였을 때 식물의 생장과 종자 발아에 미치는 영향을 조사하였다. 환경부고시 오염물질 배출기준치를 기준 농도로 하고 이보다 10배 및 50배 높은 농도의 납을 각각 처리한 애기장대의 줄기생장을 조사한 결과 오염물질 배출기준 농도와 이보다 10배 높은 농도에서는 줄기 생장이 정상식물보다 약 18%정도 감소되었다. 또한 오염물질 배출기준 농도보다 50배 높은 농도를 처리한 식물에서는 정상식물보다 약 41% 줄기생장이 감소하였다. 3가지 농도의 납을 애기장대에 처리한 후 식물의 뿌리 생장을 조사한 결과 오염물질 배출기준농도를 처리한 식물의 뿌리 생장은 정상식물과 거의 유사하였으나 오염물질 배출기준농도의 10배 이상이 되면 뿌리 생장이 전혀 되지 않았다. 3가지 농도의 납을 처리한 배지에서 애기장대의 종자 발아율을 측정한 결과 오염물질 배출기준 농도와 이보다 10배 높은 농도를 처리한 배지에서는 종자 발아율이 100%로 나타나 정상배지에서의 발아율과 차이가 나타나지 않았으나 오염물질 배출기준 농도보다 50배 높은 농도를 처리한 배지에서는 발아율이 0%로 나타나 종자 발아가 전혀 되지 않았다. 이러한 결과는 토양 속에 침적된 납은 애기장대의 생장과 종자 발아에 영향을 미치어 줄기 및 뿌리의 생장을 감소시켰으며, 또한 종자 발아에도 매우 치명적인 영향을 미치는 것으로 생각된다.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.229-235
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• 2007

Artificial seeds were produced by encapsulation of somatic embryos of Kalopanax septemlobus and investigated the effects of alginic acid concentration, size of somatic embryos, additives in capsules and nursery seedbeds for germination. The most suitable concentration of alginic acid was 3% for germination of encapsulated seeds. Germination was suppressed at higher concentration more than 3% alginic acid. For germination of artificial seeds, 1/2 MS medium with 0.02% activated charcoal was effective. There was no significant differences on the germination among the different size of somatic embryos. Additives in hydrated capsule was very important for germination and post-germinative growth of artificial seeds. Germination was severly inhibited in hydrated capsule containing only distilled water. Both sucrose and MS medium addition in hydrate capsule was effective for germination of artificial seeds. When artificial seeds were transferred to soilbed, germination rate was high in perlite containing 3% sucrose but very low in perlite with only water. These results indicate that nursery additives in both hydrate capsules and soilbeds was important for germination of artificial seeds in Kalopanax septemlobus.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.216-221
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• 2004

Changes in physiological functionality of giant embryonic rice by germination process were investigated using 70% ethanolic extract of the rices. Physiological functionality was evaluated by determining the reducing power, phenolic compound content, GABA content, and antimutagenicity. The results showed that the order of reducing power of the non-germinated rices was Nampung giant embryonic rice>normal rice> Whachung giant embryonic rice, however, the activity was high as the order of Whachung giant embryonic rice> Nampung giant embryonic rice> normal rice by germination process. About 3-fold activity increase was observed for Whachung giant embryonic rice, while, the activity of Nampung giant embryonic rice and normal rice decreased by the same treatment. The phenolic compound content of three rice cultivars were found to be almost same levels. Germination of rice increased the content of phenolic compounds by 2.6-fo1d without any considerable changes for both Nampung giant embryonic rice and normal rice. The GABA contents was highest in Whachung giant embryonic rice, followed by Nampung giant embryonic rice, normal rice in either germination or non-germination condition. The germination increased the GABA contents by more than 2.4-fold for all rice cultivars tested. We also found an increase in the antimutagenic activity by germination process for all cultivars, where the activity was the greatest for Whachung cultivar.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.257-262
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• 1997

Intergeneric crosses over 34 cross combinations between genus Brassica and Raphanus were made. Ovules taken out of crossed were cultured on MS media supplemented with 1.0 mg/L GA and BA. Germination of embryo from ovule culture was not influenced by BA and GA in medium but by parental characters in its cross combination. Use of Raphanus sativus cv. Daibyosobudore and Jungkukcheongpi showed low embryo germination when they were used as male part. Cross combination with Brassica juncea as male parent showed slightly increased germination compared to other cross. These results indicated that embryo germination in ovule culture was not much influenced by casein hydrolysate, malt extract, BA, kinetin and glutamine in the medium, but parents in combination were key factor for increasing embryo germination.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.05a
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• pp.258-260
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• 2004

4가지 중금속 중 카드뮴과 크롬은 환경부고시 오염물질 배출기준농도의 50배 이상 농도에서도 애기장대의 종자발아에 아무런 영향을 미치지 않았다. 구리는 오염물질 배출기준농도의 10배 이상, 납은 50배 이상에서 종자 발아에 치명적인 영향을 주어 발아가 전혀 일어나지 않았다. 종자 발아가 전혀 되지 않은 농도의 납(15, 20, 25 및 30 mg/L)과 발아율 10% 미만인 구리(15 및 20 mg/L)용액에 맥반석가루(3 g/30 ml)를 첨가하여 실온에서 24시간 처리한 용액에서는 100%의 종자 발아율을 나타내었다. 한편 100% 종자 발아가 된 증류수와 구리 5mg/L농도에 녹차(0.2 g/30 ml)를 첨가하였을 때에는 발아가 전혀 일어나지 않았다.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.222-227
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• 2005

To evaluate the antimutagenic activity of the specialty rices, a giant embryonic rice and a pigmented rice, we measured the inhibitory effect on the chemically induced mutagenesis in E. coli and V79 cultured cell system, as well as on DNA strand scission induced by oxidative damages in vitro. When the inhibitory activity to mitomycin C-induced mutagenesis using SOS chromotest in E. coli cell was measured, the activities decreased in the following order: germinated pigmented rice (40.4%) > germinated giant embryonic rice (37.1%) > pigmented rice (35.5%) > germinated brown rice (15.7%) > giant embryonic rice (14.0%) > brown rice (0.8%). The activities for inhibiting mitomycin C-induced DNA strand scission decreased in the order of pigmented rice > giant embryonic rice > germinated pigmented rice > germinated brown rice > brown rice > germinated giant embryonic rice. We also determined antimutagenic activities of the specialty rices using the suppressing effect on 6-TG resistant colony formation by 4-NQO in V79 cells as a mutagenicity index. The order of antimutagenicity was germinated giant embryonic rice (53.2%) > pigmented rice (40.0%) > brown rice (21.2%) > germinated brown rice (14.4%) > giant embryonic rice (0.23%); in contrast, germinated pigmented rice showed promoting effect on 4-NQO-induced mutagenesis.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.100-104
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• 2014

This study was conducted to determine the optimal MS medium strength to improve sprouting rate of apical meristem of strawberry 'Seolhyang' in vitro. Strawberry apical meristems at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the MS media with four strength levels, ($1/4{\times}$, $1/3{\times}$, $1/2{\times}$, and $1{\times}$) and the sprouting rate and growth characteristics were evaluated after eight weeks after cultivation. Shoot rate of 'Daewang' apical meristems was 93.6%whereas 'Seolhyang' apical meristems were sprouted with 31.6% on $1{\times}$ MS medium strength. Different sprouting rates were observed in 'Seolhyang' apical meristem with 31.6% in $1{\times}$ medium, 75.0% in $1/2{\times}$ medium, and 94.4% in $1/3{\times}$ medium. The sprouting rate was improved with the decrease of medium strength, but the shoot rate in $1/4{\times}$ medium decreased up to 54.5%. Shoot length was 0.9 cm in $1{\times}$ medium, 1.2 cm in $1/2{\times}$ medium, 1.6 cm in $1/3{\times}$ medium, and 1.9 cm in $1/4{\times}$ medium. Shoot length was longer as medium strength decreased and numbers of leaves and roots were not significant differences among the medium strengths. As a result, sprouting rate was highest and plant growth was best in $1/3{\times}$ MS medium compared to the others.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.3
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• pp.384-394
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• 1996

The experiment was conducted to determine the seed characteristics and preferable methods to enhance the seed germination rate in Bupleurum falcatum. The optimum temperature for the seed germination of Bupleurum falcatum is 2$0^{\circ}C$. Any significant promoting effects were not found in seed germination with hormone treatments and physical methods. At 15$^{\circ}C$, prechilling combined with 50~200ppm of GA$_3$ treatment raised germination rate by 2 times of control ones. The most positive effect was observed in the treatment of 10$^{-2}$ ~10$^{-3}$ M potassium nitrate only at 15$^{\circ}C$ for 12 and 24 hours. The leachate of Bupleurum falcatum didn't inhibit the germination of Lactuca sativa, showing almost 100% of germination rate, which is suggested that no inhibitors contained in the seeds of Bupleurum falcatum. Observation of embryo conditions under stereoscopic microscope showed that the ratio of seeds with or without embryo is almost 50/50. The results suggested that the lower rate of germination in Bupleurum falcatum was caused by embryolessness of seeds.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1735-1741
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• 2004

Preparation conditions for germinated brown rice gruel on its sensory quality were optimized using response surface methodology. Water volume ratio to germinated brown rice significantly affected the sensory quality of germinated brown rice gruel. Sensory quality, such as color, flavour, and overall acceptance increased significantly when milk ratio was increased. R2 of response surface equations were 0.9076, 0.9634, 0.9555, 0.9677 and 0.9769 for color, viscosity, flavour, mouthfeel and overall acceptance respectively (p<0.01). Optimum preparation conditions on overall acceptance for germinated brown rice gruel were: water volume ratio 8.26, cooking time 31.98 min, cooking temperature 95.71$^{\circ}C$ and milk ratio 0.54. High correlation was found between viscosity, flavour, mouthfeel, and overall quality.