• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.121-127
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• 1994

A procedure for the direct detection of (1-3)-$\beta$-glucanase isozymes in electrophoresis gels was developed. The procedure employed the commercial preparation of AZCL-pachyman as a chromogenic substrate for (1-3)-$\beta$-glucanases. The procedure detected the three basic isozymes which have been known to be expressed in germinating barley kernels. A major acidic and a minor isozymes were also detected in germinating kernels. The procedure was proved to be fast, simple and sensitive enough to be used for the analysis of the expression of (1-3)-$\beta$-glucanase isozymes in plant tissues. The detection limit of the procedure for the commercial preparation of Penicillium (1-3)-$\beta$-glucanase was estimated to be as low as 50$\mu$U. The procedure could be used for the investigation of (1-3)-$\beta$-glucanases in laboratories facilitated with ordinary equipments and research personnel.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.3
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• pp.1-7
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• 2010

Cognitive Radio, which adaptively utilizes the vacant licensed spectrum band, is considered as an effective way to alleviate the scarcity of spectrum resource shortage. In order to guarantee the non-interference transmission of primary system, spectrum sensing, especially in quiet period, is proposed. However, it is insufficient to avoid the unacceptable interference caused by Cognitive Radios, because the primary user may appear anytime that is unpredictable. In this paper, we address the deficiency of conventional spectrum sensing and propose a novel Cognitive Radio receiver structure with monitoring function block to detect the appearance of primary user in a real-time manner. Simulations prove that the proposed eigenvalue based detection method together with the two-threshold decision procedure performs properly.

• Journal of the Korean Chemical Society
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• v.52 no.2
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• pp.155-163
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• 2008

.A simple and reproducible method for the rapid extraction and determination of trace amounts of copper(II) ions using mesoporous organo-silicas mesoporous silica and atomic absorption spectrometry is presented. Common coexisting ions did not interfere with the separation and determination. The preconcentration factor was 100 (1 ml elution volume) for a 100 ml sample volume. The limit of detection of the proposed method is 1.0 ng ml-1. The maximum sorption capacity of sorbent under optimum conditions has been found to be 5mg of copper per gram of sorbent. The relative standard deviation under optimum conditions was 2.8% (n=10). Accuracy and application of the method was estimated by using test samples of natural and synthetic water spiked with different amounts of copper(II) ion.

• Journal of KIISE
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• v.41 no.12
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• pp.1013-1017
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• 2014

A variety of applications that are accessible through app markets provide useful features and functions. However, those applications can present many GUI bugs due to the deficiency of testing processes. Even though various approaches have been developed for mobile app testing, GUI bugs in applications are still difficult to be identified due to the absence of efficiency, lack of automation, and necessity of access to the source code. In this paper, we propose an automated black-box testing method for efficient GUI bug detection. Our experimental results show that the proposed method achieves better code coverage and uncovers GUI bugs when compared with existing black-box testing called Monkey.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.454-460
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• 2016

In this study, we investigated the possibility of Droplet digital PCR (ddPCR) for detection of foodborne pathogens. ddPCR combines partitioning of PCR reactions into several thousands or millions of individual droplets in a water-oil emulsion, and counting of positive PCR reaction using flow cytometry. Four species of foodborne pathogens, Bacillus cereus, Staphylococcus aureus, Salmonella Typhimurium and Escherichia coli O157:H7, were used to quantify the target sequence with each of the designed primers and double stranded DNA-binding Evagreen dye. All tested foodborne pathogens showed a detection limit ranging from $100fg/{\mu}L$ to $10ng/{\mu}L$. It was concluded that ddPCR could be used to detect very low concentrations of foodborne pathogens from complex food matrices. For multi-detection of target pathogens, we also tested the samples using multiplex ddPCR and obtained successful results.

• Proceedings of the Korea Information Processing Society Conference
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• 2017.11a
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• pp.924-926
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• 2017

본 논문에서는 좁고 곡률이 큰 도로에서의 자율 주행을 위한 AVM 시스템 기반의 알고리즘을 제안한다. 기존의 전방을 주시하는 모노/스테레오 카메라를 이용한 차선 인식 방법을 이용한 자율주행 알고리즘은 모노/스테레오 카메라의 제한된 FOV (Field of View)로 인해 좁고 곡률이 큰 도로에서의 자율 주행에 한계가 있다. 제안하는 알고리즘은 AVM 시스템을 기반으로 하여 이 한계를 극복하고자 한다. AVM 시스템에서 얻은 영상을 차선의 색상 정보를 이용해 차선의 영역을 이진화 한다. 이진화 영상으로부터, 차량의 뒷바퀴 주변의 관심영역을 시작으로 재귀적 탐색법을 이용하여 좌, 우 차선을 검출한다. 검출된 좌, 우 차선의 중앙선을 차량의 경로로 삼고 조향각을 산출해 낸다. 제한하는 알고리즘을 실제 차량에 적용시킨 실험을 수행하였고, 운전면허 시험장의 코스를 차선의 이탈없이 주행 가능함을 실험적으로 확인하였다.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.11
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• pp.821-826
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• 2011

Synthetic musk compounds are widely used as fragrances in a variety of personal care products like soaps, sampoos, cosmetics and perfumes. The occurrence of synthetic musk compounds in municipal sewage effluent and other environmental samples could negatively impact the health of the ecosystem and humans, due to persistent and long-term chronic exposure of aquatic organisms. Fifteen synthetic musk compounds (musk ketone, musk xylene, musk ambrette, musk moskene, musk tibetene, HHCB, AHTN, ADBI, AHDI, DPMI, ATII, ethylenebrasssylate, ambretettolide, cyclopentadecanolide, OTNE) were analysed in surface waters and sewage treatment plants (STP) influents/effluents in Korea by GC/MS. Method detection limits were $0.005{\sim}0.398{\mu}g/L$. HHCB and AHTN were most frequently observed in both surface waters and STP influents/effluents.

• Journal of KIISE
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• v.42 no.6
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• pp.699-706
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• 2015

In recent years, as near-duplicate image has been increasing explosively by the spread of Internet and image-editing technology that allows easy access to image contents, related research has been done briskly. However, BoF (Bag-of-Feature), the most frequently used method for near-duplicate image detection, can cause problems that distinguish the same features from different features or the different features from same features in the quantization process of approximating a high-level local features to low-level. Therefore, a post-verification method for BoF is required to overcome the limitation of vector quantization. In this paper, we proposed and analyzed the performance of a post-verification method for BoF, which converts SIFT (Scale Invariant Feature Transform) descriptors into 128 bits binary codes and compares binary distance regarding of a short ranked list by BoF using the codes. Through an experiment using 1500 original images, it was shown that the near-duplicate detection accuracy was improved by approximately 4% over the previous BoF method.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.4
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• pp.333-340
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• 2019

This study attempted to eatablish a high performance liquid chromatography (HPLC) analysis method for the determination of avicularin, quercitrin as a part of the quality control for the development of functional cosmetic materials from Astilbe chinensis extract. HPLC was performed on a Capcell Pak C₁₈ MGII column with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at 30 ℃. The analyte was detected at 254 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation of avicularin and quercitrin were 0.094 and 0.285 mg/mL, 0.031 and 0.095 mg/mL respectively. Calibration curves showed good linearity r² > 0.99990 for avicularin and r² > 0.99994 for quercitrin. Precision of analysis was satisfied with less than 0.59% for avicularin and 0.63% for quercitrin. Recoveries of quantified compounds ranged from 100.97 to 101.77% for avicularin and 100.18 to 100.32% for quercitrin. These result indicated that the established HPLC method is very useful for the determination of marker compounds in A. chinensis extracts.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.87-93
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• 2019

This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison $US-C_{18}$ column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity ($r^2$ > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.