• Title/Summary/Keyword: 발아미

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Antioxidant Activity of Ethanolic Extract from Germinated Giant Embryonic Rices (발아 거대배아미 에탄올 추출물의 항산화 활성)

  • Kang, Mi-Young;Kim, Sul-Yi;Koh, Hee-Jong;Nam, Seok-Hyun
    • Applied Biological Chemistry
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    • v.47 no.3
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    • pp.293-299
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    • 2004
  • Antioxidant activity of ethanolic extract of two giant embryonic rices, Nampung giant embryonic rice and Hwachung giant embryonic rice, were investigated mainly focusing on their ability to inhibit lipid peroxidation and scavenge reactive oxygen species in comparison with those of general rice. The results showed that germination process increased reducing power, inhibitory activity on lipid peroxidation and scavenging ability either on superoxide or hydroxyl radicals. Among the cultivars tested, increase in antioxidative action was found to be most prominent for Hwachung giant embryonic rice cultivar. For scavenging of superoxide radicals, the extract from Nampung giant embryonic rice has the most potent activity, however, increasing rate of scavenging activity by germination process was also found to be the greatest for Hwachung giant embryonic rice. We found that the scavenging mechanism for superoxide radicals was attributed to the direct scavenging of the radicals. The scavenging of hydroxyl radicals, the most toxic oxygen radical to biological system, by the rice extracts were also examined, and the results showed that either overall activity or the increasing rate of the activity to scavenge hydroxyl radicals by germination process was the greatest for Hwachung giant embryonic rice. Moreover, the results suggested that the scavenging action to hydroxyl radicals might be mediated by direct quenching of the radicals, not by chelating $Fe^{2+}$. Further studies showed that the antioxidant action of the rice extracts tested in vitro was also operative for suppressing ROS production induced in TPA-stimulated HL-60 cells.

Characteristics of White Soybean Chungkookjang Fermented by Bacillus subtilis D7 (Bacillus subtilis D7에 의하여 발효된 백태 청국장의 특성)

  • Lee, Na-Ri;Park, Sung-Bo;Lee, Sang-Mee;Go, Tae-Hun;Hwang, Dae-Youn;Kim, Dong-Seob;Jeong, Seong-Yun;Son, Hong-Joo
    • Journal of Life Science
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    • v.23 no.4
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    • pp.529-536
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    • 2013
  • This study was carried out to investigate the characteristics of white soybean Chungkookjang fermented by Bacillus subtilis D7. The highest germination rate was obtained under $25^{\circ}C$ when water was supplied for 4 days at intervals of 2 hr. The total isoflavone content was 971.3 ${\mu}g/g$ before germination and 1023.8 ${\mu}g/g$ after germination. The amino type- and ammonia type-nitrogen contents of Chungkookjang were proportional to the fermentation time. The pH values of all Chungkookjang soybeans increased up to pH 7.8-8.0 during fermentation. The number of viable cells in all Chungkookjang soybeans increased significantly up to 24 hr. The protease activity of all Chungkookjang soybeans increased up to 30-36 hr. The ${\alpha}$- and ${\beta}$-amylase activities of Chungkookjang fermented by B. subtilis D7 were enhanced with increasing fermentation time. Analysis of the effect of Chungkookjang intake on the liver function of mouse showed that the alkaline phosphatase (ALP) activity and the superoxide dismutase (SOD) activity in the Chungkookjang diet group were markedly higher than those in the control group. The asparatate aminotransferase (AST) activity in the germinated soybean Chungkookjang diet group was higher than that in the nongerminated soybean Chungkookjang diet group. Therefore, Chungkookjang soybeans fermented with B. subtilis D7 can be expected to have an increased content of functional components and improved quality characteristics.

Differences in Physicochemical and Textural Properties of Germinated Brown Rice in Various Rice Varieties (발아현미의 이화학적 특성 및 취반 특성)

  • Cho, Dong-Hwa;Park, Hye-Young;Lee, Seuk-Ki;Park, Jiyoung;Choi, Hye-Sun;Woo, Koan-Sik;Kim, Hyun-Joo;Sim, Eun-Yeong;Won, Yong-Jae;Lee, Dong-Hyun;Oh, Sea-Kwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.3
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    • pp.172-183
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    • 2017
  • Germinated brown rice (GBR) has received considerable attention over the last decade as a means of enhancing the nutritional value and health-promoting functions of rice. The effect of germination on the physicochemical and textural properties of brown rice (BR) was investigated in different rice varieties (Samkwang, Misomi, Chindeul, and Hyeonpum). Cooking properties, such as water absorption, expanded volume, and soluble solids were significantly increased by germination in all rice varieties. Textural properties (hardness, toughness, adhesiveness, and stickiness) of cooked samples were determined using a texture analyzer. Hardness and toughness were decreased by germination, whereas stickiness and adhesiveness increased significantly. These results revealed that germination leads to improvements in the cooking and eating properties of BR. In Rapid Visco Analyzer (RVA), significant reductions were observed for peak viscosity, break down, set back, and final viscosity after germination. Although the amylose content of Misomi was slightly decreased by germination, that of other varieties increased significantly. Germination induced no noticeable change in the average chain length of amylopectin in Misomi, Chindeul and Hyeonpum, but led to a significant increase in Samkwang.

Effect of Pre-Harvest Sprouting on Seed Viability, Germination and Seedling Emergence Rate of Rice (벼 수발아가 종자 활력, 발아율 및 입모율에 미치는 영향)

  • Shon, Jiyoung;Kim, Junhwan;Jung, Hanyoung;Kim, Bo-Kyung;Choi, Kyung-Jin;Yang, Woonho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.427-434
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    • 2014
  • This study aimed to investigate the effects of pre-harvest sprouting (PHS)-damaged grains of rice on seed viability, germination percentage and seedling emergence rate. As comparing the seed lots of same cultivar 'Hopum' with different PHS percentages, seed lot with 8% PHS (high PHS percentage) showed 30% lower germination than that with 1% PHS (low PHS percentage). The difference of seedling emergence rate and seed viability percentages between the two seed lots was consistent with that of germination percentage. PHS-damaged brown rice was observed protruded embryo, discolored endosperm and fungi infected grains. PHS grains were classified into two groups, PHS-I showing endosperm discoloration in one third of a grain and PHS-II in more than half of a grain. The seed lot with high PHS percentage had much more PHS-II grains than that of low PHS percentage. PHS-II grains showed remarkably lower germination percentage than PHS-I grains and got severely moldy during germination test. In laboratory experiment, morphological changes and re-germination ability of seeds which dried after germination by soaking were tested. Sprouted seeds of 3mm shoot length showed half discolored endosperm and had a strong resemblance with PHS-II grains. Re-germination percentage of 3mm sprouted seeds sharply decreased than 2 mm sprouted seeds. Sprouted seeds which were treated for accelerated-aging (AA) for 24hr at $45^{\circ}C$ were significantly reduced in re-germination percentage compared to that of non-AA-treated sprouted seeds. These results indicate that PHS with over 3mm shoot length can severely damage not only grain appearance but also seed viability, germination percentage and seedling emergence rate. Therefore, we conclude that larger difference of germination percentage or seedling emergence rate between seed lots having smaller difference of PHS percentage might be caused by accelerated seed deterioration in PHS-damaged rice grains.

Preparation and Characterization of Wet Noodle Containing Germinated Small Black Bean Flour (발아약콩가루를 첨가한 생면의 제조 및 특성연구)

  • Han, Sung-Mi;Han, Jung-Ah
    • Korean Journal of Food Science and Technology
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    • v.43 no.5
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    • pp.597-602
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    • 2011
  • Small black beans (SBB) were germinated up to 72 h at 23$^{\circ}C$, and 48 h germinated SBBs having the highest isoflavone content were chosen for noodle preparation. Some of the germinated SBB were treated by 20 min boiling (B) but some were not boiled (NB). For noodles, the germinated SBB flour replaced 15% or 20% of wheat flour, so five kinds of noodles were prepared and tested: control, 15B, 15NB, 20B, and 20NB. Higher amount of bean flour resulted in significant decrease of $L^*$ and increase of $b^*$ values in noodle. After 20 min cooking, the noodles containing germinated SBB flour showed less soluble sugar content in cooked water than control, and the lowest value was observed in 15B. For textural properties, the addition of SBB flour lowered hardness and adhesiveness of noodle, but increased chewiness than control. In sensory tests, the most acceptable sample was 15B because of developed texture and better appearance which seem to be good for health.