• Title/Summary/Keyword: 발사-발사-확인

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Optimization of a protocol for the production of transgenic lily plants via particle bombardment (유전자총 실험조건 최적화를 통한 형질전환 백합 식물체 생산)

  • Kim, Jong Bo
    • Journal of Plant Biotechnology
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    • v.44 no.1
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    • pp.82-88
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    • 2017
  • Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and $1.0{\mu}m$ of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.

Study on Developing Instrument System for Measuring Action time of K4 Grenade Machine Gun for Improving Quality Assurance on 40mm High Velocity Grenade (40mm 고속유탄의 품질보증 향상을 위한 K4 기관총의 Action Time 계측시스템 개발에 관한 연구)

  • Hong, Sung-Kook;Shin, Jun-Goo;Jeon, Hye-Jin;Kim, Yong-Hwa;Ju, Jin-Chun;Kwon, In-Gyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.7
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    • pp.4828-4834
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    • 2015
  • From the moment that a firing pin triggers the detonator to the moment that a grenade leaves a barrel is called Action Time. Since a loading and percussion of 40mm grenade happens simultaneously, action time should be within a certain time in order to prevent a Jamming malfunction. Previously, unreliable action time device of 40mm grenade made it difficult to improve quality assurance of K4 Grenade Machine Gun. Here, various sensors were compared and a special device was designed to seek an accurate measurement on action time. In this device, the gap between a signal from an optical sensor in Firing Pin and that from Eddy current probe in the barrel was recorded and data were sent to a computer in real time. Confirming if action time is within the criteria, it is expected that action time plays an important role in quality assurance on 40mm grenade.

Estimation of Fire Emissions Using Fire Radiative Power (FRP) Retrieved from Himawari-8 Satellite (히마와리 위성의 산불방사열에너지 자료를 이용한 산불배출가스 추정: 2017년 삼척 및 강릉 산불을 사례로)

  • Kim, Deasun;Won, Myoungsoo;Lee, Yangwon
    • Korean Journal of Remote Sensing
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    • v.33 no.6_1
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    • pp.1029-1040
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    • 2017
  • Wildfires release a large amount of greenhouse gases (GHGs) into the atmosphere. Fire radiative power (FRP) data obtained from geostationary satellites can play an important role for tracing the GHGs. This paper describes an estimation of the Himawari-8 FRP and fire emissions for Samcheock and Gangnueng wildfire in 6 May 2017. The FRP estimated using Himawari-8 well represented the temporal variability of the fire intensity, which cannot be captured by MODIS (Moderate Resolution Imaging Spectroradiometer) because of its limited temporal resolution. Fire emissions calculated from the Himwari-8 FRP showed a very similar time-series pattern compared with the AirKorea observations, but 1 to 3 hour's time-lag existed because of the distance between the station and the wildfire location. The estimated emissions were also compared with those of a previous study which analyzed fire damages using high-resolution images. They almost coincided with 12% difference for Samcheock and 2% difference for Gangneung, demonstrating a reliability of the estimation of fire emissions using our Himawari-8 FRP without high-resolution images. This study can be a reference for estimating fire emissions using the current and forthcoming geostationary satellites in East Asia and can contribute to improving accuracy of meteorological products such as AOD (aerosol optical depth).

Automatic Discharge Measurement Using the Velocity Index Method (유속지수법을 이용한 자동유량측정)

  • Kim, Chi-Young;Kim, Won;Lee, Chan-Joo;Kim, Dong-Gu
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.242-246
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    • 2006
  • 최근 들어 전 세계적으로 유량측정 분야의 큰 변화의 방향은 자동화이다. 전자, 전기 기술과 정보통신 기술의 발달을 유량측정 분야에 적용하여 자동적으로 유량측정을 수행하고 실시간으로 모든 유량자료를 수집하는 시스템을 구성하려는 연구가 활발하게 진행중이다. 최근에 초음파 유량계와 더불어 자동 유량측정 기법으로 각광을 받고 있는 기법이 유속지수법(index velocity method)이다. 유속지수법의 원리는 매우 간단하다. 수위 기록을 통하여 수위-면적 관계로부터 흐름 단면적을 구하고, 임의의 영역에서 측정된 유속이 단면 평균 유속으로 환산될 수 있다면, 흐름 단면적과 평균 유속에 의해 연속적으로 유량을 구할 수 있다. 유속지수법에서 가장 중요한 것이 전체 평균 유속을 대표할 수 있는 유속지수를 정확하고 효율적으로 측정하는 것이다. 유속지수법에 의한 연속 유량측정 목적으로 최근에 ADVM(Acoustic Doppler Velocity Meter)이 개발되어 이용되고 있다. ADVM은 수중에 초음파를 발사해서 산란체에서 반사되어 돌아오는 초음파의 주파수 편이, 즉 도플러 효과를 이용하여 유속을 측정하는 유속계이다. 본 연구에서는 ADVM을 괴산댐 하류에 위치한 시험하천에 설치하여, 유속지수법에 의한 유량측정기법을 적용하고 그 특성을 분석하였다. 유속지수법으로 측정된 유량을 괴산댐 방류량과 비교한 결과 평균 4.0%의 상대오차를 지니고 있어 비교적 정확한 연속 유량측정이 가능한 것으로 나타났다. 이와 같은 유속지수법을 하천 유량측정에 활용하면 보다 정확한 유량을 연속적으로 자동화하여 측정할 수 있을 것으로 기대된다.연계모형의 한계로 인하여 두 모형의 통합모형이 필요하다. 즉, 강우 혹은 월류유량으로 발생한 지표유량 중 일부분이 과부하가 발생하지 않는 유입구 지점을 통과할 때 배수시스템으로 유입되는 것을 고려할 수 있고, 유입된 유량은 배수시스템 내의 흐름에 반영되도록 배수시스템과 침수해석모형을 통합한 모형 개발이 필요하다. 그러기 위해서는 지표면과 배수시스템에 대한 수리학적 관계를 정립하여야 한다. 본 연구에서는 배수시스템 해석 모형과 도시침수해석 모형을 통합하고, 두 모형간의 유량의 전송과정을 수리학적 관계를 고려한 dual-drainage 도시침수해석모형을 개발하였다. 이를 위해 도시지역 배수시스템 해석 모형으로 널리 이용되고 있는 SWMM모형을 이용하여 지표면으로의 월류량을 산정하고 유입된 지표유량에 대해서 배수시스템에서의 흐름해석을 수행하였다. 그리고, 침수해석을 위해서는 2차원 침수해석을 위한 DEM기반 침수해석모형을 개발하였고, 건물의 영향을 고려할 수 있도록 구성하였다. 본 연구결과 지표류 유출 해석의 물리적 특성을 잘 반영하며, 도시지역의 복잡한 배수시스템 해석모형과 지표범람 모형을 통합한 모형 개발로 인해 더욱 정교한 도시지역에서의 홍수 범람 해석을 실시할 수 있을 것으로 판단된다. 본 모형의 개발로 침수상황의 시간별 진행과정을 분석함으로써 도시홍수에 대한 침수위험 지점 파악 및 주민대피지도 구축 등에 활용될 수 있을 것으로 판단된다. 있을 것으로 판단되었다.4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기

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Validation of Surface Reflectance Product of KOMPSAT-3A Image Data Using RadCalNet Data (RadCalNet 자료를 이용한 다목적실용위성 3A 영상 자료의 지표 반사도 성과 검증)

  • Lee, Kiwon;Kim, Kwangseob
    • Korean Journal of Remote Sensing
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    • v.36 no.2_1
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    • pp.167-178
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    • 2020
  • KOMPSAT-3A images have been used in various kinds of applications, since its launch in 2015. However, there were limits to scientific analysis and application extensions of these data, such as vegetation index estimation, because no tool was developed to obtain the surface reflectance required for analysis of the actual land environment. The surface reflectance is a product of performing an absolute atmospheric correction or calibration. The objective of this study is to quantitatively verify the accuracy of top-of-atmosphere reflectance and surface reflectance of KOMPSAT-3A images produced from the OTB open-source extension program, performing the cross-validation with those provided by a site measurement data of RadCalNet, an international Calibration/Validation (Cal/Val) portal. Besides, surface reflectance was obtained from Landsat-8 OLI images in the same site and applied together to the cross-validation process. According to the experiment, it is proven that the top-of-atmosphere reflectance of KOMPSAT-3A images differs by up to ± 0.02 in the range of 0.00 to 1.00 compared to the mean value of the RadCalNet data corresponding to the same spectral band. Surface reflectance in KOMPSAT-3A images also showed a high degree of consistency with RadCalNet data representing the difference of 0.02 to 0.04. These results are expected to be applicable to generate the value-added products of KOMPSAT-3A images as analysisready data (ARD). The tools applied in thisstudy and the research scheme can be extended as the new implementation of each sensor model to new types of multispectral images of compact advanced satellites (CAS) for land, agriculture, and forestry and the verification method, respectively.

RF Compatibility Test using RF Suitcase (이동형 RF 시험장비를 이용한 RF 호환성 시험)

  • Kim, Eung-Hyeon;Jeong, Dae-Won;Kim, Hui-Seop;Im, Jeong-Heum;Lee, Sang-Jeong
    • Journal of Satellite, Information and Communications
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    • v.1 no.2
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    • pp.45-50
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    • 2006
  • A satellite and ground stations which are developed in a program are tested whether the interface between the satellite and ground is well established before satellite operations. These compatibility tests are performed when the satellite is connected with the ground stations after all satellite and ground stations requirements are verified. The content of the RF compatibility test is to check whether the interface requirements which are described on the Interface Control Document are well developed. During the early operation phase and tentative contingency operations of the satellite, KARI ground station uses other oversea ground stations which are located worldwide according to contract between the KARI and the contractor. Since oversea ground stations were not developed for the designated space program, system integrator should check whether the oversea ground stations are satisfied with interface requirements. Using the RF suitcase, RF interface and the content of RF communication can directly be verified during RF compatibility test on oversea ground station without KARI ground station's support. The RF compatibility test using RF suitcase was performed oversea ground stations as well as KARI ground station located on Korea. The content of RF compatibility test was standardized in order to be used at any oversea ground stations, especially fitted for the operations concept of launch and early operations phase. The test content would be RF characteristics, protocol, command loop test, telemetry loop test, and ground station interface test.

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The Effect of Indwelling Silk Suture Material Following Aspiration in the Treatment of Chronic Prepatellar Bursitis (흡인 후 견 봉합사 거치를 통한 만성 슬개골전 점액낭염의 치료)

  • Lee, Bong-Jin;Lee, Sung-Rak;Kim, Chung-Hyun;Kim, Seong-Tae
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.4 no.1
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    • pp.55-59
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    • 2005
  • Purpose: To evaluate the drainage effect of silk suture material following aspiration of the bursa as an early treatment of chronic prepatellar bursitis. Materials and Methods: Twelve cases, which have over two weeks of history and over one year of follow-up, were investigated. The average duration of follow-up was 18.3 months. The average symptom duration before introduction into this study was 2.2 months. With an aseptic technique, the aspiration of the bursa was done with spinal needle or injection needle and syringe and then the insertion of silk suture material through the aspiration needle was performed. Over one year follow-up, recurrence, infection, pain, and limitation of range of motion were investigated by telephone interview. Results: Redness around the insertion site of silk suture material was found in all cases, but there was no development of active infection in eleven cases. At five days after procedure, a supprative infection was developed in one case. The results were considered satisfactory in 92% of cases and the average duration of treatment is 14.5 days. Conclusion: The drainage with silk suture material following aspiration of the bursa is effective and less invasive method in the early treatment of chronic prepatellar bursitis.

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MANUFACTURING AND TEST RESULTS OF OFF-AXIS PARABOLIC CYLINDER MIRROR FOR FIMS (FIMS에 사용되는 비축 포물 원통형 반사경의 제작과 성능 시험 결과)

  • Ryu, K.-S.;Yuk, I. S.;Seon, K.-I.;Lee, Y.-W.;Nam, U.-W.;Shin, J.-H.;Hong, S.-J.;Lee, D.-H.;Jin, H.;Oh, S.-H;Rhee, J.-G.;Min, K.-W.;Han, W.;Park, J.-H.;Edelstein, J.;Korpela, E. J.
    • Journal of Astronomy and Space Sciences
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    • v.18 no.3
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    • pp.239-248
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    • 2001
  • Far-ultraviolet IMaging Spectrograph (FIMS) is the main payload of the first Korean scientific satellite, KAISTSAT-4, which will be launched in 2002. Among the optical parts, parabolic cylinder mirror does not have any heritage from previous astronomical missions, so the manufacturing and testing process itself is a challenging issue. We describe the method of manufacturing and measuring of the off-axis parabolic cylinder mirror and our initial experiments to establish the entire manufacturing process. Using the method, the profile error can meet the specification of $lambda$ per cm which is closely related with the astronomical performances. In case of the surface roughness, temperature controlled pitch polishing reduces $R_{q}$ under 1 nm implying that scattering in the entire spectral range of FIMS is less than 2% of the incident UV light.

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Inland ASF Measurement by Signal of the 9930M Station (9930M국 로란-C 신호를 이용한 내륙 ASF 측정 연구)

  • Yang, Sung-Hoon;Lee, Chang-Bok;Lee, Jong-Koo;Kim, Young-Jae;Lee, Sang-Jeong
    • Journal of Navigation and Port Research
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    • v.34 no.8
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    • pp.603-607
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    • 2010
  • The LORAN system had been used widely and it was an essential navigation aid for ships in the ocean until the GPS is adopted actively. In particular, it was essential functionality for the ships to sail the oceans. According to the advancement of industry, however, the current accuracy of traditional Loran is insufficient for the utilization of harbour approach, land navigation, and the field of survey and timing. Therefore it is necessary that the study on the improvement of the positioning accuracy of Loran. The one of the improving methods is to measure and compensate the propagation time delay between the transmitter and user's receiver, which is called as additional secondary factor (ASF). In this study, we measured the ASF between the Pohang master transmitting station (9930M) and four points where locate within 33 km apart from the transmitting station, using the measuring technique of the absolute time delay without a time of coincidence (TOC) table. As the result of measurement, the ranging error caused by the propagation delay was about 210 m at 33 km, however it can be reduced up to 40 m with ASF compensation.

Interferometric coherence analysis using space-borne synthetic aperture radar with respect to spatial resolution (공간해상도에 따른 위성 영상레이더 위상간섭기법 긴밀도 분석)

  • Hong, Sang-Hoon;Wdowinski, Shimon
    • Korean Journal of Remote Sensing
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    • v.29 no.4
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    • pp.389-397
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    • 2013
  • Recently high spatial resolution space-borne Synthetic Aperture Radar (SAR) systems have launched and have been operated successfully. Interferometric SAR (InSAR) processing with the space-based high resolution observations acquired by these systems can provide more detail information for various geodetic applications. Coherence is regarded as a critical parameter in the evaluating the quality of an InSAR pair. In this study, we evaluate the coherence characteristics of high-resolution data acquired by TerraSAR-X (X-band) and ALOS PALSAR (L-band) and intermediate-resolution data acquired by Envisat ASAR (C-band) over western Texas, U.S.A. Our coherence analysis reveals that the high-resolution X-band TSX (3.1 cm) data has a high coherence level (0.3-0.6), similar to that of the L-band ALOS PALSAR data (23.5 cm) in short temporal baselines. Further more, the TSX coherence values are significantly higher than those of the C-band (5.6 cm) Envisat ASAR data. The higher coherence of the TSX dataset is a surprising result, because common scattering theories suggest that the longer wavelength SAR data maintain better coherence. In vegetated areas the shorter wavelength radar pulse interacts mostly with upper sections of the vegetation and, hence, does not provide good correlation over time in InSAR pairs. Thus, we suggest that the higher coherence values of the TSX data reflect the data's high-resolution, in which stable and coherent scatters are better maintained. Although, however, the TSX data show a very good coherence with short temporal baseline (11-33 days), the coherences are significantly degraded as the temporal baselines are increased. This result confirms previous studies showing that the coherence has a strong dependency on the temporal baseline.