• 제목/요약/키워드: 반코마이신 내성 장구균

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Detection of Enterococci and their Vancomycin Resistance in Drinking Spring-Water (먹는물 약수터 장구균의 검출 특성과 반코마이신 내성 현황)

  • Yoon, Tae-Ho;Lee, Hyang;Lee, Seung-Joo;Yeo, In-Hak;Eom, Seok-Won
    • Journal of Korean Society of Environmental Engineers
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    • 제32권10호
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    • pp.979-985
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    • 2010
  • This study was performed to detect enterococci strain as an indicator of faecal contamination, to identify of 16S rDNA sequence and vancomycin resistance by MIC (Minimum Inhibitory Concentration) test from drinking spring-water samples in Seoul. The detection frequency of enterococci was 42 (19.8%) among 212 samples, and its concentration was ranged from 0 to 110 CFU/100 mL. These results were confirmed the possibility as an indicator microorganisms that similar to the frequency of E. coli detection (t test p-value 0.268, significant level 0.05). Isolated 56 enterococci samples were identified by 16S rDNA sequence data and their NCBI BLAST searching. They were identified to Enterococcus faecalis of 24 samples, E. faecium (10), E. casseliflavus (10), E. gallinarum (3), E. hirae (2), E. durans (2), E. sanguinicola (1). E. faecalis was dominant species that clinical case report of a domestic was similar. Vancomycin resistant enterococci (VRE) of 53 samples showed that vanB and vanC1/C2 type with 2 and 12 case, respectively. These results indicated that the drinking spring-water quarantined to fecal pollution for block out outbreak of gastrointestinal symptom with using such as disinfection process.

Factors Influencing Level of Awareness and Compliance with Vancomycin-Resistant Enterococcus Infection Control among Nurses in Intensive Care Units (중환자실 간호사의 반코마이신 내성 장구균 감염관리에 대한 인지도, 이행도 및 관련요인)

  • Park, Young-Mi;Park, Hyoung-Sook;Park, Kyung-Yeon
    • Journal of Korean Academy of Fundamentals of Nursing
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    • 제15권4호
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    • pp.531-538
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    • 2008
  • Purpose: The purpose of this study was to identify factors influencing Vancomycin-resistant enterococcus infection control among nurses in intensive care units. Method: Data were collected from August 15 to October 14, 2007 from 188 nurses working in intensive care units. The nurses answered a 26 item-questionnaire, which included management of the cohort (14 items), hand washing (6 items) and management of the environment (6 items). Descriptive statistics, t or F test, ANOVA, and multiple regression analysis were used with SPSS PC+ 14.0 to analyze the data. Results: The participant's level of awareness of Vancomycin-resistant enterococcus infection control was 3.87; that of compliance was 3.74. Significant factors influencing the level of compliance with Vancomycin-resistant enterococcus infection control were'the level of the awareness' and 'the type of intensive care unit'. These two variables accounted for 21.0% of variance for compliance with the Vancomycin-resistant enterococcus infection control among the participants. Conclusion: In order to develop a strategy to increase the compliance with Vancomycin-resistant enterococcus infection control, it is necessary to be concerned about 'the level of the awareness', 'the type of intensive care unit;', and 'experiences of caring for patients with Vancomycin-resistant enterococcus'.

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Distribution Characteristics and Antibiotics Resistance of Enterococcus spp. in Nakdong River (낙동강에서 엔테로코커스의 분포특성 및 항생제별 내성률)

  • Seong, Jin-Uk;Cha, Soon-Bae;Ryu, Kwang-Hyun;Choi, Kwang-Soon;Park, Je-Chul
    • Korean Journal of Ecology and Environment
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    • 제46권3호
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    • pp.360-366
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    • 2013
  • This study was it conducted on effluent or river water close to discharge locations from a treatment plant, which were analyzed for the presence, phenotype and antibiotic resistance rates of Vancomycin Resistant Enterococci (VRE). The test results for isolation and identification of Enterococcus spp. showed that they all were VRE positive, with a total of 32 strains detected. Multiplex PCR was conducted for 32 strains, each of which were identified as E. faecium, and the results indicated that they were all confirmed as VRE, corresponding to the Van A phenotype. The results of E. faecium concentrations measured at various locations indicated that they were, on average, higher at the location of sewage treatment plants. The frequency of positive tests as well as the number of colonies was higher downstream of treatment plants. The minimum inhibitory concentration was inspected for 26 strains of discharged water samples from sewage water treatment plants, and 6 strains of river samples. Out of 19 antibiotics, 14 and 5 species showed resistance and sensitivity, respectively.

A Case of Vancomycin-Resistant Enterococci Peritonitis in a Pediatric Patient on CAPD Successfully Treated with Linezolid (지속성 외래 복막투석 소아에서 리네졸리드로 치료한 반코마이신 내성 장구균 복막염 1례)

  • Baek, Seung-Ah;Park, Sung-Sin;Kim, Sung-Do;Cho, Byoung-Soo
    • Childhood Kidney Diseases
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    • 제12권2호
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    • pp.245-249
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    • 2008
  • Peritonitis is one of the major complications of CAPD(continuous ambulatory peritoneal dialysis). Recently, multidrug-resistant organisms, such as vancomycin-resistant enterococcus(VRE) have been rarely reported by the pathogen as of CAPD-associated peritonitis. But, there is limited information on choices of effective therapy for VRE peritonitis in patients undergoing CAPD. We present a pediatric case of successful treatment of CAPD-associated peritonitis due to VRE with linezolid, and review of the literature.

Risk Factors and Clinical Outcomes for Vancomycin-Resistant Enterococcus Colonization on Intensive Care Unit Admission (중환자실 환자의 입실시 반코마이신 내성 장구균 집락의 위험요인과 임상적 결과)

  • Byun, Sook-Jin;Kang, Jiyeon
    • Journal of Korean Academy of Nursing
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    • 제43권2호
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    • pp.287-295
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    • 2013
  • Purpose: The purpose of this study was to identify vancomycin-resistant enterococcus (VRE) colonization rate in patients admitted to the intensive care unit (ICU), associated risk factors and clinical outcomes for VRE colonization. Methods: Of the 7,703 patients admitted to the ICUs between January, 2008 and December, 2010, medical records of 554 VRE colonized and 503 uncolonized patients were reviewed retrospectively. To analyzed the impact of colonization on patients' clinical outcomes, 199 VRE colonized patients were matched with 199 uncolonized patients using a propensity score matching method. Results: During the study period, 567 (7.2%) of the 7,703 patients were colonized with VRE. Multivariate analysis identified the following independent risk factors for VRE colonization: use of antibiotics (odds ratio [OR]=3.33), having bedsores (OR=2.92), having invasive devices (OR=2.29), methicillin-resistant Staphylococcus aureus co-colonization (OR=1.84), and previous hospitalization (OR=1.74). VRE colonized patients were more likely to have infectious diseases than uncolonized patients. VRE colonization was associated with prolonged hospitalization and higher mortality. Conclusion: Strict infection control program including preemptive isolation for high-risk group may be helpful. Further research needs to be done to investigate the effects of active surveillance program on the incidence of colonization or infection with VRE in the ICU.

Uncertainty and Performance of Infection Control in Caregivers of Vancomycin-Resistant Enterococci Patients (반코마이신 내성 장구균 환자 보호자의 질병 불확실성과 감염관리 수행)

  • Lee, Eunsuk
    • The Journal of the Korea Contents Association
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    • 제15권6호
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    • pp.346-357
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    • 2015
  • Purpose: This descriptive study was to examine uncertainty and performance of infection control in the VRE patients' caregivers. Methods: The participants were 82 caregivers who involved with the VRE patients care in D tertiary hospital in D metropolitan city. Data were analyzed with number, percentage, t-test, and ANOVA using SPSS/Win 21.0. Results: The participants' uncertainty was 56.99 and performance of infection control was 35.09. Performance of infection control was significantly different by age (F=121.38, p<.001), education (F=102.77, p<.001), relationship with the patient (F=17.80, p<.001), hours of caring per day (t=3.14, p=.002), and type of family (t=-8.65, p<.001). There was a significant negative correlation between participants' uncertainty and performance of infection control (r=-.96, p<.001). Conclusion: The results of the study will be used to develop nursing intervention program and standard protocol for infection control for the VRE patients' caregivers.

Rapid Detection of Vancomycin Resistant Enterococci Using Multiplex Polymerase Chain Reactions (다중 중합효소 연쇄반응을 이용한 반코마이신 내성 장구균의 신속 검출)

  • 김종배;김근희;송혜원;박성언;엄용빈;박상욱;김양수;박수진
    • Biomedical Science Letters
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    • 제5권1호
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    • pp.95-100
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    • 1999
  • It is generally difficult, time-consuming, and expensive for the clinical laboratory to detect vancomycin resistant enterococci (VRE). The aim of this study was to develop and evaluate the multiplex polymerase chain reaction (PCR) assay system as a diagnostic tool for the rapid detection of VRE from clinical samples and/or for the identification of VRE from the bacterial strains isolated from clinical specimens. Specific primers, designed from the nucleotide sequences respectively encoding the vanA, vanB, vanC-1, vanC-2/3 genes in enterococci, were coupled in a multiplex PCR assay system. With this multiplex PCR assay system, we investigated the incidence rates and types of VRE isolated from clinical samples. A total of 75 strains of enterococci were isolated in 3 general hospitals in Korea. Of these isolates, 36 strains showed a pattern of high-level vancomycin resistance which associated with vanA gene, whereas 18 strains showed low-level vancomycin resistance associated with vanC-1 or vanC-2/3 gene. Thus, multiplex PCR assay method established in this study could be applied for the rapid detection of VRE.

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The Factors Influencing Compliance of Multidrug-resistant Organism Infection Control in Intensive Care Units Nurses (중환자실 간호사의 다제내성균 감염관리 수행에 영향을 미치는 요인)

  • Kim, Ji Hee;Lim, Kyung Hee
    • Korean Journal of Adult Nursing
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    • 제27권3호
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    • pp.325-336
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    • 2015
  • Purpose: This study was conducted to identify factors influencing compliance of multidrug-resistant organism infection control in intensive care units (ICU) nurses. Methods: Data were collected from 254 ICU nurses who were working at 6 general and advanced general hospitals in D city and G Province. Results: 77.2% and 84.4% of the subjects correctly answered to questions about Methicillin-Resistant Staphylococcus Aureus (MRSA) and Vancomycin-Resistant Enterococcus (VRE), respectively. The scores of MRSA infection control compliance and VRE infection control compliance were 3.41 and 3.43, respectively. The factors influencing MRSA infection control compliance were empowerment, environmental safety recognition, and education satisfaction, which explained 30% of MRSA infection control compliance. The factors significantly related to VRE infection control compliance were empowerment, hospital types, environmental safety recognition, number of education sessions, and neonatal ICU, which explained 37% of VRE infection control compliance. Conclusion: It is necessary to develop efficient educational programs for infection control including educational contents to improve empowerment and environmental safety recognition of nurses. Furthermore, administrative support for those infection control programs is also necessary.

Structure Determination of Macrolactin Compounds with Antibacterial Activities Isolated from Bacillus polyfermenticus KJS-2 (Bacillus polyfermenticus KJS-2에서 분리된 항생물질 마크로락틴류의 구조결정)

  • Kim, Dong-Hee;Kang, Kyung-Ran;Kim, Hyun-Woo;Yoon, Si-Yeol;Kim, Chun-Gyu;Yamaguchi, Tokutaro;Sohng, Jae-Kyung;Kang, Jae-Seon
    • Journal of Life Science
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    • 제20권12호
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    • pp.1792-1800
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    • 2010
  • In this study, we isolated five macrolactin compounds from a fermentation broth of Bacillus polyfermenticus KJS-2. The macrolactin compounds were structurally identified as macrolactin A (MA), 7-O-malonyl macrolactin A (MMA), 7-O-succinyl macrolactin A (SMA), macrolactin E (ME) and macrolactin F (MF) via a variety of NMR techniques, COZY, DEPT, HMQC and HMBC, and mass and specific rotation assays. The three macrolactin compounds, MA, MMA and SMA, profoundly inhibited the growth of both vancomycin-resistant Enterococci (VREs) and methicillin-resistant Staphylococcus aureus (MRSA), the inhibition of which were estimated via a disc agar diffusion bioassay. MA, MMA, and SMA exhibited antibacterial activities superior to those of vancomycin and teicoplanin.