• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.470-474
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• 2003

DNA sequence information on small-subunit rRNA gene (16S rDNA) obtained from food-poisoning bacterial culture was used to investigate the presence of bacterial pathogens in food. By reverse dot blot detection method, presence of food-poisoning bacteria could be confirmed on hybridization of digoxigenin-labeled 16S rDNA Polymerase Chain Reaction (PCR) primer product and biotin-labeled specific oligonucleotide probe. Escherichia coli, Bacillus cereus. and Salmonella sp. were used as the representative food-poisoning bacterial microorganisms. An oligonucleotide probe, based on the variable region of 16S rRNA gene, was used as the specific probe. These tools may be more useful than classic biochemical method for rapid identification of contaminated food.

• Restorative Dentistry and Endodontics
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• v.13 no.2
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• pp.265-282
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• 1988

The pulp response of posterior composite resins in relation to the thickness of remaining dentin was studied with 120 teeth from 6 dogs, Class V. cavities were prepared on the cervical area of facial surfaces. The thickness of remaining dentin was controlled with Caries Meter$^{(R)}$. The cavities of group A were prepared to show the electrical impedance of 22-26$K{\Omega}$(thickness of remaining dentin:0.4-0.5mm). The cavities of group B, 50-55$K{\Omega}$(thickness of remaining dentin: 0.8-0.9mm). Zinc - Oxide Eugenol cement, Estilux$^{(R)}$ posterior, Heliomolar$^{(R)}$ radiopaque, P-30$^{(R)}$ and Scotchbond$^{(R)}$+P-30$^{(R)}$ were filled in each cavity. After 3days, 1 week, 2 weeks, 4 weeks, 9 weeks and 13 weeks, the teeth and pulp tissue were processed routinely and stained with Hematoxylin and Eosin. Pathological tissue changes were observed with light microscope. The following results were obtained. I. The pulp response of group A cavties was severer than that of group B cavities. 2. In the pulp of group A cavities which were filled with Zinc-Oxide Eugenol Cement, only vascular changes were observed after 3 days and 1 week, severe acute inflammation after 4 weeks, moderate acute inflammation after 9 weeks, and chronic inflammation and formation of granulation tissue after 13 weeks. 3. In the pulp of group A cavities which were filled with Estilux$^{(R)}$ posterior, only vascular changes were observed after 3 days and 1 week. But the inflammatory response has became much severer with the elapsed experimental period. 4. In the pulp of group A cavities which were filled with Heliomolar$^{(R)}$ radiopaque, the inflammatory response with the elapsed experimental period was not severer than that of the pulp of group A cavities which were filled with other materials. 5. In the group B cavities, the difference of pulp response by filling materials was not recognizable. In the group A cavities, the pulp response of Estilux$^{(R)}$ posterior was severest and in order P-30$^{(R)}$, Heliomolar$^{(R)}$ radiopaque was slighter.

• KSBB Journal
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• v.28 no.2
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• pp.65-73
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• 2013

This study aimed to investigate the enzymatic hydrolysis of mannitol using Viscozyme$^{(R)}$ L, Celluclast$^{(R)}$ 1.5 L, Saczyme$^{(R)}$, Novozym$^{(R)}$, Fungamyl$^{(R)}$ 800 L, Driselase$^{(R)}$ Basidiomycetes sp., and Alginate Lyase, and to optimize of reaction conditions for production of reducing sugar. Response surface methodology (RSM) based on central composite rotatable design was used to study effects of the independent variables such as enzyme (1-9% v/w), reaction time (10-30 h), pH (3.0-7.0) and reaction temperature ($30-70^{\circ}C$) on production of reducing sugar from mannitol. The coefficient of determination ($R^2$) of $Y_1$ (yield of reducing sugar by Viscozyme$^{(R)}$ L) and $Y_3$ (yield of reducing sugar by Saczyme$^{(R)}$) for the dependent variable regression equation was analyzed as 0.985 and 0.814. And the p-value of $Y_1$ and $Y_3$ showing 0.000 and 0.001 within 1% (p < 0.01), respectively, was very significant. The optimum conditions for production of reducing sugar with Viscozyme$^{(R)}$ L were 9.0 % (v/w) amount of enzyme, 30.0 hours of reaction time, pH 4.5 and $30.0^{\circ}C$ of reaction temperature, and those with Saczyme$^{(R)}$ were 9.0% (v/w) of amount of enzyme dosage, 30.0 h of reaction time, pH 7.0 and $30.0^{\circ}C$ of reaction temperature, consequently, the predicted reducing sugar yields were 22.5 and 27.9 mg/g-mannitol, respectively.

• Childhood Kidney Diseases
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• v.3 no.1
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• pp.27-34
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• 1999

Purpose: This study was designed to investigate the changes in soluble interleukin-2 receptor (sIL-2R) level in sera and urines of children with primary nephrotic syndrome, eliminating the confounding effects of age, proteinuria, and steroid treatment. Methods: Soluble IL-2R was measured by ELISA in sera and urines from patients with minimal change nephrotic syndrome or focal segmental glomerulosclerosis as well as from healthy controls. The serum levels and urinary sIL-2R/creatinine ratios were compared between control group and the 12 patient groups divided by their ages (0-1, 2-4, over 5 years), and presence or absence of proteinuria and/or steroid treatment (PU+Tx-, PU+Tx+, PU-Tx+, PU-Tx-). Results: Though the differences were not statistically significant probably because of the small numbers, serum sIL-2R levels seemed to be higher in younger age groups both in patients and control group. Nephrotic children did not show higher serum levels than normal children. Among the patients, proteinuric condition seemed to raise and steroid treatment tended to suppress the serum sIL-2R levels. Urinary sIL-2R/creatinine ratios were higher in younger age groups, more significantly in patients (P<0.001). Proteinuria and steroid treatment affected the urinary sIL-2R/creatinine ratios by the same way as the serum sIL-2R levels. Serum sIL-2R levels and urinary sIL-2R/creatinine ratios were not different between groups of different histologic findings or steroid responsiveness (P>0.05). Conclusion: Serum sIL-2R levels and the urinary sIL-2R/creatinine ratios were higher in younger age, and they were not higher in nephrotic patients compared to control group. The patients in relapse showed higher levels, while the levels were suppressed with steroid treatment. In proteinuric state, urinary sIL-2R/creatinine ratios reflected serum sIL-2R levels.

• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.18-23
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• 2012

Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-$Kill^{(R)}$ composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-$Kill^{(R)}$ and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-$Kill^{(R)}$. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-$Kill^{(R)}$. As Citra-$Kill^{(R)}$ possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.3
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• pp.241-247
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• 2001

Boundary line method was adopted to analyze the relationships between rice yield and meteorological conditions during rice growing period. Boundary lines of yield responses to mean temperature($T_a$) and sunshine hour( $S_{h}$) and diurnal temperature range($T_r$) were well-fitted to hyperbolic functions of f($T_a$) =$$\beta$_{0t}$(1-EXP(-$$\beta$_{1t}$ $\times$ ($T_a$) ) and f( $S_{h}$)=$$\beta$_{0t}$((1-EXP($$\beta$_{1t}$$\times$ $S_{h}$)), to quadratic function of f($T_r$) =$\beta$$_{0r}$(1-($T_r$ 1r)$^2$), respectively. to take into account to, the sterility caused by low temperature during reproductive stage, cooling degree days [$T_c$ =$\Sigma$(20-$T_a$] for 30 days before heading were calculated. Boundary lines of yield responses to $T_c$ were fitted well to exponential function of f($T_c$) )=$\beta$$_{0c}$exp(-$$\beta$_{1c}$$\times$$T_c$ ). Excluding the constants of $\beta$$_{0s}$ from the boundary line functions, formed are the relative function values in the range of 0 to 1. And these were used as yield indices of the meteorological elements which indicate the degree of influence on rice yield. Assuming that the meteorological elements act multiplicatively and independently from each other, meteorological yield index (MIY) was calculated by the geometric mean of indices for each meteorological elements. MIY in each growth period showed good linear relationship with rice yield. The MIY's during 31 to 45 days after transplanting(DAT) in vegetative stage, during 30 to 16 days before heading (DBH) in reproductive stage and during 20 days after heading (DAH) in ripening stage showed greater explainablity for yield variation in each growth stage. MIY for the whole growth period was calculated by the following three methods of geometric mean of the indices for vegetative stage (MIVG), reproductive stage (HIRG) and ripening stage (HIRS). MI $Y_{I}$ was calculated by the geometric mean of meteorological indices showing the highest determination coefficient n each growth stage of rice. That is, (equation omitted) was calculated by the geometric mean of all the MIY's for all the growth periods devided into 15 to 20 days intervals from transplanting to 40 DAH. MI $Y_{III}$ was calculated by the geometric mean of MIY's for 45 days of vegetative stage (MIV $G_{0-45}$ ), 30 days of reproductive stage (MIR $G_{30-0}$) and 40 days of ripening stage (MIR $S_{0-40}$). MI $Y_{I}$, MI $Y_{II}$ and MI $Y_{III}$ showed good linear relationships with grain yield, the coefficients of determination being 0.651, 0.670 and 0.613, respectively.and 0.613, respectively.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.195-200
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• 2006

The response of hepatic mixed function oxygenase (MFO) system was investigated in olive flounder exposed to formalin. Hepatic microsome of olive flounder incubated in vitro with formalin demonstrated the induction of cytochrome P450 (CYP), ethoxyresorufin deethylase (EROD), cytochrome P450 reductase (P450R) and cytochrome b5 reductase (b5R) activity. In addition, olive flounder was exposed to 100, 300 and 500 ppm of formalin for 1 h and then transferred to a flow-through type of 1000 L aquarium. Hepatic MFO enzyme activity was determined for 72 h. As the result, hepatic CYP, P450R and EROD activities increased following exposure of formalin, but b5R and GST showed no significant change. These results imply that CYP and P450R can be considered as main hepatic enzymes involving in detoxification of formalin.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.3
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• pp.69-73
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• 2010

The melt grafting of unsaturated silanes onto polypropylene (PP) in a twin-screw extruder and crosslinking in hot water were studied to enhance impact strength of polypropylene. The influence of grafting formulations on the melt flow rates of grafted PP and the gel percentages of crosslinked PP was investigated. 3-methacryloylpropyltrimethoxysilane (VMMS) unsaturated silane monomer was used. Benzoyl peroxide, (BPO) and Dicumyl peroxide (DCP) were used as an initiator. When benzoyl peroxide (BPO) was used as an initiator, higher gel percentage and impact strength than those of DCP has been observed. The maximum impact strength was obtained with 0.7 phr of BPO and 2phr of VMMS. The value is 8.7 kgf-cm/cm and it is on a parity with the value of with 20 phr of EOR mixed to PP.

• Journal of the Korean Ceramic Society
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• v.31 no.2
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• pp.220-226
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• 1994

The hydrolysis and condensation reactions in the mixed alkoxide solutions of Si(OC2H5)4 and Zr(O-nC3H7)4 with various water contents (1, 2, 4, and 8 in molar ratio to alkoxide, r) and catalysts were examined by rheological measurements and the number average molecular weight in order to explain the shape of the polymer in the mixed alkoxide solutions. It was found that fibers could be drawn in the viscosity range of 1∼100P from the acid-catalyzed solutions with lower water contents of the mole ratio H2O/alkoxide, r 2. On the other hand, crack free bulk gel was formed from the acid-catalyzed solutions including a large amount of water (r 4), and the base-catalyzed solutions. The relation between the intrinsic viscosity [{{{{ eta }}] and the number average molecular weight n, namely [{{{{ eta }}]=Knα, has shown that the acid-catalyzed spinnable solutions (r=1 and 2) have linear polymers and the exponent α's are about 0.56 and 0.81, whereas non-spinnable solutions (r=4 and 8) have three dimensional network polymers or spherical particles and the exponent α's are 0.41∼0.51 and 0.35.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.120-124
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• 2004

본 연구는 Diluted Ceria 입자를 사용한 $SiO_2$(Oxide) CMP 현상에 대한 내용이다. Ceria Slurry의 경우 Silica Slurry와 비교하였을 때 Oxide Wafer 표면과 축합 화학반응을 일으키며 Chemistry Dominant한 CMP Mechanism을 따르고, Wafer Center Removal Rate(RR) Fast 의 특성을 가진다. Ceria Slurry의 문제점인 연마 불균일도를 해결하기 위해 Tribological System을 이용하였다. CMP Tribology는 Pad-Slurry 유막-Wafer의 System을 가지며 윤활막에 작용하는 마찰계수(COF)가 주요 인자이다. Tribology에 적용되는 Stribeck Curve를 통해 Slurry 윤활막의 두께(h) 정도를 예상할 수 있으며, 이 윤활막의 두께를 조절함으로써 Uniformity 향상이 가능하다. 이 Ceria Slurry CMP의 연마 불균일도를 향상시킬 수 있는 방법으로 pH 조절 및 점도 증가가 있다. Ceria 입자 CMP는 분산액의 pH 변화에 강한 작용을 받게 되며 PH5 근방에서 최적화된 Uniformity가 가능하다. 점도를 증가시키는 경우 유막 h가 증가하게 되어 Ceria Slurry의 유동이 균일 분포 상태에 가까워지며 Wafer Uniformity 향상이 가능하다.