• Title/Summary/Keyword: 반응기작

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Partial Correlation between Hydrological, Geochemical and Microbiological Processes in Groundwater-stream Water Mixing Zone in a Rural Area (농촌지역 지하수-지표수 혼합구간에서 수리, 지구화학 및 생물학적 기작 사이의 편상관분석)

  • Kim, Heejung;Lee, Jin-Yong;Lee, Kang-Kun
    • Journal of Wetlands Research
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    • v.14 no.4
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    • pp.489-502
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    • 2012
  • Biogeochemical processes in groundwater-stream water mixing zone are recently of great interest because biodegradation and natural attenuation of aquatic contaminants may occur through the processes. The objectives of this study are to investigate the hydrologic and biogeochemical processes at the groundwater-stream water mixing zone through which surface water-driven nitrate may be naturally attenuated, and to examine the effect of the vertical flow exchange flux on biogeochemical processes using correlation analysis. To examine the direction of vertical water flow in the zone, vertical hydraulic gradients were measured at several depths using mini-piezometers. Microbial populations in soil samples of the zone were also analyzed by means of the polymerase chain reaction (PCR) and Cloning methods. In addition, partial correlations among vertical flow exchange, nitrate concentration and microbial activity was investigated to examine their mutual interaction. The results showed the significant interaction among the three parameters, resulting in natural attenuation of nitrate. This study showed an example of the biogeochemical fuction of groundwater-stream water mixing zone, which can be predictable from the examination of the interaction among microbial activities, concentration of contamination and vertical flow exchange flux. temperature show a significant difference in adjacent streambed, Also, the results shows that distribution of temperature was more affected by groundwater direction than intensity of flux.

Thermal Kinetics of Color Changes of Purple Sweet Potato Anthocyanin Pigment (자색고구마 Anthocyanin 색소의 가열에 대한 속도론적 연구)

  • Lee, Lan-Sook;Rhim, Jong-Whan
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.497-501
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    • 1997
  • Kinetic parameters on heat-induced color changes of anthocyanin pigment from purple sweet potato were determined in the temperature range of $121{\sim}141^{\circ}C$. Color change determined by a browning index $(A_{532}\;nm/A_{420}\;nm)$ followed second order reaction kinetics. Activation energy values of purple sweet potato pigment solutions of pH 2.0, 3.0, 4.0 and 5.0 were 69.57, 76.68, 81.07 and 92.98 kJ/mol, respectively, indicating that temperature dependency of the reaction increased with pH. Apparent kinetic compensation effect between preex-ponential factor and activation energy value was observed.

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Preliminary Experiments for the Remediation of Trichloroethene-Contaminated Groundwater Using Direct-Current and Zero-Valent Iron (0가 철과 직류전원을 이용한 TCE 오염 지하수의 정화기법 예비조사)

  • Moon, Ji-Won;Moon, Hi-Soo;Roh, Yul;Lee, Suk-Young;Song, Yun-Goo
    • Economic and Environmental Geology
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    • v.34 no.3
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    • pp.307-313
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    • 2001
  • Reactive medium including zero-valent metals such as zero-valent iron ($Fe^0$) degrades chlorinated solvents as a contaminant plume flows through the treatment medium. Although the Feo based reactive barrier has been demonstnlted to be a cost effective for trichloroethenc (TCE)-contaminaled plume remediation, current approach is limited by low process eftlciency and uncertain, effective life of the medium. The objective of this study is to develop an enhanced treatment method of TeE-contaminated groundwater using Feo and direct current. The bench-scale test using flow-through $Fe^0$ reactor column confirmed that the application of direct current with $Fe^0$ is highly effective in enhancing the rate of TeE dechlorination. The dechlorination mechanism appears to be reductive, with the electrons supplied by the iron oxidation and external power supply serving as the additional source of electrons.

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The Change of Peroxidase Activity in Soybean Seed Followed by Infection with Cercospora kikuchii (대두종자의 자반병 감염과 Peroxidase 활성도변화)

  • Park W.M.;Ko Y.H.;Yoo Y.J.;Lee J.Y.
    • Korean journal of applied entomology
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    • v.21 no.1 s.50
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    • pp.23-26
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    • 1982
  • The present study was carried out to investigate the change of peroxidase activity of soybean seed infected with Cerrospora kikurhii. The protein content, polyphenol oxidase activity and peroxidase isozyme pattern in health and infected soybean seed were also compared. 1. The peroxidase activity was substantially higher in the infected soybean seeds than that in the healthy seeds either cracked or not. No significant differences in protein content were recognized among the seeds tested. 2. No significant differences in peroxidase activities and protein contents were notified between healthy and infected seeds from the measurements on each parts of dissected seeds, cotyledon and seedcoat, however the peroxidase activity in the seed coat of the stained seed was 2.5 times to healthy seed. 3. The activities of polyphenel oxidase were undectable in both healthy and diseased seeds. 4. The electrophoretic Patterns of the Peroridase isozyme were the same between healthy and in footed seed. 5. Therefore, the increase of peroxidase activity in infected soybean seedcoat was mainly due to the biochemical reaction against the pathogen.

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Study on Anti-estrogenic Activity of DEHP as an Endocrine Disruption Chemical (내분비 교란성 DEHP의 항-에스트로젠 활성에 관한 연구)

  • Kim, Eun-Joo
    • Journal of Environmental Health Sciences
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    • v.29 no.2
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    • pp.7-15
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    • 2003
  • Di-2-ethylhexyl phthalate (DEHP), is a widely used plasticizer known to be a suspected endocrine disrupter, but its exact effects on aquatic organisms are not yet known. When Japanese medaka (Oryzias latipes) were exposed from the time of hatching to 3 months of age to an aqueous DEHP solution at nominal concentrations of 1, 10, and 50 $\mu\textrm{g}$/l, DEHP treated female fish showed distinct reproductive effect. And the midge (Chironomus riparius.). an aquatic invertebrate, was exposed to DEHP to evaluate the effects on reproductive processes via sediment toxicity. The test endpoints included emergence, sex ratio, fecundity, and the viability of F1 offspring egg ropes. The result implied that the normal developmental and/or reproductive processes in C. riparius had been disrupted when exposed to DEHP, the effect also being displayed in the next generation. In summary, DEHP hinders the development of reproductive organs in the female Japanese medaka and C. riparius.

The Roles of Protein Degradation During Fungal-plant Interactions (단백질 분해가 식물의 진균 병 진전에 미치는 영향)

  • Ahn, Il-Pyung;Park, Sang-Ryeol;Bae, Shin-Chul
    • The Korean Journal of Mycology
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    • v.38 no.2
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    • pp.89-94
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    • 2010
  • Plant pathogenic fungi are the most diverse and drastic causal agents of crop diseases threatening stable food production all over the world. Plant have evolved efficient innate immune system to scout and counterattack fungal invasion and pathogenic fungi also developed virulence system to nullify plant resistance machinery or signaling pathways and to propagate and dominate within their niche. A growing body of evidences suggests that post translational modifications (PTMs) and selective/nonselective degradations of proteins involved in virulence expression of plant pathogenic fungi and plant defense machinery should play pivotal roles during the compatible and incompatible interactions. This review elucidates recent investigations about the effects of PTMs and protein degradations on host defense and fungal pathogens' invasions.

Therapeutic strategies to manage chronic wounds by using biofilm dispersal mechanisms (생물막 분산기작을 이용한 만성창상의 치료전략)

  • Kim, Jaisoo;Kim, Min-Ho
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.87-102
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    • 2019
  • Most chronic wounds persist in the inflammatory phase during wound healing due to the biofilm. Biofilms are resistant to antibiotics, weakening penetration, resistance to biocides and weakening local immune responses. The biofilm is firmly attached to the surrounding tissues and is very difficult to remove. Therefore, strategies to remove hard biofilms without damaging surrounding tissue are very important. One of possible strategies is dispersal. So many studies have been done to develop new strategies using dispersal mechanisms. In this review paper, especially chemotaxis, phage therapy, polysaccharides, various enzymes (glycosidases, proteases, and deoxyribonucleases), surfactants, dispersion signals, autoinducers, inhibitors were introduced. Combination therapies with other therapies such as antibiotic therapy were also introduced. It is expected that the possibility of treatment of chronic wound infection using the knowledge of the biofilm dispersal mechanisms presented in this paper will be higher.

Study of the possible mode of action of O-ethyl S-methyl ethylphosphonothioate via the formation of S-oxide in chemical and metabolic oxidation systems (화학적, 대사적 산화반응 중 생성되는 S-oxide를 이용한 O-ethyl S-methyl ethylphosphonothioate (1) 의 독성 기작에 관한 연구)

  • Hur, J.H.;Fukuto, T.R.;Han, D.S.
    • Korean Journal of Environmental Agriculture
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    • v.10 no.2
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    • pp.167-177
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    • 1991
  • O-ethyl S-methyl ethylphosphonothioate [$LD_{50}$ (rat, oral) 4.6mg/kg ; $K_i$(bovine erythrocyte acetylcholinesterase) 303 $M^{-1\;min-1}$] was selected as a model compound to study the mode of action of O, S-dialkyl alkylphosphonothioates which have been hypothesized to be toxic via a bioactivation process. Two chemical oxidants, meta-chloroperoxybenzoic acid and monoperoxyphthalic acid, and rat liver microsomal oxidases were used to mimic the action of mixed function oxidases on the model compound. The formation of S-oxide, a very unstable active intermediate, was proposed based on the identification of metabolic products.Furthermore, a trapping experiment with ethanol showed that the unstable intermediate S-oxide had the ability to phosphorylate acetylcholinesterase which is an important enzyme in nerve systems. The S-oxide intermediates are presumed to be responsible for the toxicity of O,S-dialkyl alkylphosphonothioates.

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Functional Mechanism of Calmodulin for Cellular Responses in Plants (식물의 세포반응에 대한 칼모듈린의 functional 작용기작 연구)

  • Cho, Eun-Kyung;Choi, Young-Ju
    • Journal of Life Science
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    • v.19 no.1
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    • pp.129-137
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    • 2009
  • Calcium ($Ca^{2+}$) plays pivotal roles as an intracellular second messenger in response to a variety of stimuli, including light, abiotic. and biotic stresses and hormones. $Ca^{2+}$ sensor is $Ca^{2+}$-binding protein known to function in transducing signals by activating specific targets and pathways. Among $Ca^{2+}$-binding proteins, calmodulin (CaM) has been well reported to regulate the activity of down-stream target proteins in plants and animals. Especially plants possess multiple CaM genes and many CaM target proteins, including unique protein kinases and transcription factors. Thus, plants are possible to perceive different signals from their surroundings and adapt to the changing environment. However, the function of most of CaM or CaM-related proteins have been remained uncharacterized and unknown. Hence, a better understanding of the function of these proteins will help in deciphering their roles in plant growth, development and response to environmental stimuli. This review focuses on $Ca^{2+}$-CaM messenger system, CaM-associated proteins and their role in responses to external stimuli of both abiotic and biotic stresses in plants.

Evaluation of Cu Removal from Mine Water in Passive Treatment Methods : Field Pilot Experiments (자연정화 기반의 현장 파일럿 실험을 통한 광산배수 구리 정화효율 평가)

  • Oh, Youn Soo;Park, Hyun Sung;Kim, Dong Kwan;Lee, Jin Soo;Ji, Won Hyun
    • Economic and Environmental Geology
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    • v.53 no.3
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    • pp.235-244
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    • 2020
  • Copper (Cu), one of the main contaminants in the mine drainage from the closed mine area, needs to be removed before exposed to environment because of its toxicity even in the low concentration. In this study, passive treatment based field pilot experiments using limestone and compost media were conducted during 9 months for enhancing Cu removal efficiency of the mine water treatment facility of S mine located in Goseong, Gyeongsangnam-do in South Korea. The pH increase and Cu removal efficiency showed high value at Successive Alkalinity Producing System ( SAPS) > Reducing and Alkalinity Producing System (RAPS) > limestone reactor in a sequence. The compost media using in SAPS and RAPS contributed to raise pH by organic material decomposition with generating alkalinity, thus, Cu removal efficiency increased. Also, experimental results showed that Cu removal efficiency was proportional to pH increase, meaning that pH increase is the main mechanism for Cu removal. Moreover, Sulfate Reduction Bacteria (SRB) was identified to be most activated in SAPS. It is inferred that the sulfate reduction reaction also contributed to Cu removal. This study has the site significance in that the experiments were conducted at the place where the mine water generates. In the future, the results will be useful to select the more effective reactive media used in the treatment facility, which is most appropriate to remediate mine water from the S mine.