• Journal of Dairy Science and Biotechnology
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• v.41 no.1
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• pp.9-25
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• 2023

Yogurt fermentation is known to be beneficial because it provides a low pH and harsh environment for foodborne pathogens and improves organoleptic properties. Additionally, organic acids produced through fermentation have a good effect on the viscosity and gelling properties of yogurt. Several potential health benefits of probiotic and generally recognized as safe strains have been suggested. Yogurt is the preferred vehicle for delivering probiotics to health-conscious consumers. Therefore, manufacturers of probiotic beverages must comply with the relevant regulations. The development of probiotic yogurt begins with the selection of strains with safety and functional properties of probiotics. The selected probiotic strain should be technically suitable for viability and improve organoleptic quality while maintaining the number of bacteria above the standard value during processing and storage conditions. In addition, the efficacy of probiotic strains contained in yogurt should be investigated, confirmed, and approved according to well-designed clinical trials. Although various methods are used to detect probiotic strains, the recently widely used next generation sequencing method can be actively utilized. In the future, more research should be conducted with the latest methods to identify probiotic functions and accurately detect probiotic strains.

• Journal of Dairy Science and Biotechnology
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• v.41 no.4
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• pp.179-190
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• 2023

Currently, various probiotics are being used to improve the nutrition of companion animals. They are widely sold as additives in companion animal foods because of the numerous gastrointestinal and immune health benefits for dogs and cats. Therefore, extensive research is being conducted to improve quality and safety during manufacturing and to extend the shelf life of companion animal foods by adding probiotics. The manufacturing process must be conducted such that the characteristics and efficacy of probiotics added to food are optimally beneficial for companion animals. Therefore, this review aims to address the overall characteristics of the probiotic strains used and to examine the various methods through which probiotics are added to companion animal foods.

• Food Science and Industry
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• v.50 no.4
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• pp.92-103
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• 2017

A companion animal is a pet which lives and shares emotions with a person. The global pet industry economy has grown in recent times due to increased awareness of companion animals. In Korea, it grew from 800 million US dollars in 2012 to 2 billion US dollars in 2016, about 2.5-fold growth in five years. Within the pet industry, the pet food market occupies the largest market share having a value greater than 70 billion US dollars globally. In China, the average annual growth rate of the pet food industry is estimated at about 30%, and the market size is expected to reach 18 billion US dollars by 2020. The companion food market has potential to be the fastest growing and most viable food market in the future and would be a strategic employment avenue within the food industry. This report articulates the necessity for product development and institutional improvement of companion animal.

• The Korean Journal of Food And Nutrition
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• v.35 no.2
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• pp.159-166
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• 2022

The purpose of this study was to explore the marketability by developing a fresh HMR product for improving hyperlipidemia and dieting for elderly chronic disease patients and young people pursuing healthy beauty. The diet menu increased the ratio of fresh vegetables and mushrooms, and chicken breast and cheese were used as protein sources. By using whole grains, the supply of vitamins and minerals was strengthened while minimizing calories. Regarding the recipe, the diet menu was mainly prepared in the form of salads, risotto, and pasta. In the hyperlipidemia improvement menu, the proportion of fresh vegetables was increased, and as protein sources, pork shank, tofu, seafood, etc. with minimal fat were used. As a carbohydrate source, whole grains were mainly used to minimize calories while strengthening the supply of vitamins and minerals. In the recipe, it was prepared in the form of steamed or bibim-myeon, and it was also produced in a form such as paella. As a result, the developed menu was analyzed as low-calorie and evenly comprised of essential nutrients, which can satisfy palatability and nutrition.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.384-397
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• 2018

The aim of this study is to screen the strains of Bacillus spp. possessing safety, probiotic activity, and so on, which can be utilized as probiotic resource for using the feed and supplement food of companion animal. About 300 isolates were isolated from traditional Korean sauces, four isolates that did not have or produce the six kinds of B. cereus type vomiting and diarrhea toxin genes, ${\beta}$-hemolytic, and three kinds of carcinogenic enzymes were selected. Antibiotic gene retention, cell surface hydrophobicity, antibiotic sensitivity, and glucose utilization were analyzed for four isolates, and finally SRCM 100731 was selected. SRCM 100731 was named as Bacillus amyloliquefaciens SRCM 100731 16S rRNA sequencing analysis, and carried out optimization of cell growth for industrial applications such as pet food and feed. The effects of 14 different components on cell growth were investigated and three significant positive factors, molasses, sodium chloride, and potassium chloride were selected as the main factors based on a Plackett-Burman design. In order to find out optimal concentration on each constituent, we carried out central composite design. The predicted optimized concentrations were 7% molasses, 1.1% sodium chloride, 0.5% potassium chloride. Finally, an overall about 7-fold increase in dry cell weight yield ($12.6625{\pm}0.0658g/L$) was achieved using the optimized medium compared with the non-optimized medium ($1.8273{\pm}0.0214g/L$). This research is expected to be highly utilized in the growing pet industry by establishing optimal cultivation conditions for industrial application as well as screening Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal.

• Journal of Food Hygiene and Safety
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• v.37 no.1
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• pp.15-20
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• 2022

The purpose of this study was to compare the conventional culture method, enzyme immune method and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domestically distributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventional culture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from the enzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR method with species-specific primer allows better distinguishment between the species of the strain collected from the samples than the conventional culture method and/or the enzyme immune method.

• Journal of Environmental Science International
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• v.32 no.2
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• pp.139-143
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• 2023

The effects of essential oils on pH, pathogens, and volatile fatty acids (VFA) in two poultry litters were investigated through a lab study. Essential oil-added poultry litters were randomly divided to two groups: control (200 g poultry litter) and Treatment (50 g thymol/Briefly, 200 g broiler litter was treated with or without 50 g thymol (Control and T1, respectively; 1 groups) and 200 g duck litter was treated with or without 50 g carvacrol (Control and T2, respectively; 2 group). Adding thymol to broiler litter increased the pH, reduced pathogens, and did not affect VFA. Interestingly, adding carvacrol slightly reduced the pH of duck litter, but had no significant effect on reducing pathogens and VFA. This difference is probably because the essential oil used and the properties of the two litters are different. In addition, pH was thought to control the odor generated from the litter, but this has not been proven. Further field studies should focus on clarifying this point.

• Journal of Environmental Science International
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• v.32 no.10
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• pp.741-744
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• 2023

This study focused on high school laboratory research and the main purpose was to develop alternative additives for livestock waste and ammonia volatilization methods with high school students as participants and to provide information to business owners based on the results. Compared to the control groups, The bentonite and illite treatment groups had similar ammonia volatilization, pH, EC, and total nitrogen content. In particular, the alum and aluminum chloride mixed treatment group showed low pH and ammonia volatilization, and high EC and total nitrogen content for poultry litter. As a result, when focusing on high school laboratory research, the alum and aluminum chloride mixed agent treatment fulfilled its role as an alternative additive for ammonia reduction. In addition, this approach can be suggested as a method to solve difficulties in adapting to the field through a practical cooperative relationship with livestock farms.

• Journal of Environmental Science International
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• v.31 no.11
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• pp.993-997
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• 2022

This study was conducted to evaluate the structure and composition (i.e., pH, moisture, total-N, pathogens, and volatile fatty acids) of broiler and duck manure treated with larvae of three insect larvae, namely, Tenebrio molitor, Protaetia brevitarsis seulensis, and Ptecticus tenebrifer. Hatched Tenebrio molitor (n=300), Protaetia brevitarsis seulensis (n=60), and Ptecticus tenebrifer (n=300) were used in this study; specially, the larvae were divided into six treatments with three replicates. The treatments were as follows: T1: 110 g broiler manure + Tenebrio molitor larvae (n=50), T2: 110 g duck manure + Tenebrio molitor larvae (n=50), T3: 125 g broiler manure + Protaetia brevitarsis seulensis larvae (n=10), T4: 125 g duck manure + Protaetia brevitarsis seulensis larvae (n=10), T5: 105 g broiler manure + Ptecticus tenebrifer larvae (n=50), and T6: 105 g duck manure + Ptecticus tenebrifer larvae (n=50). For all the larval treatments, the following results were observed: The moisture content of the duck manure treat with three insect larvae was higher than that of the broiler manure (p<0.05), whereas broiler manure had a higher pH (p<0.05). In addition, the total nitrogen content of broiler manure was higher than that of duck manure (p<0.05). However, the insect larvae did not significantly affect pathogens (E.coli and Salmonella) and the volatile fatty acids (p>0.05). In conclusion, the use of the three insect larvae to create organic nitrogen compost using poultry manure is feasible.

• Journal of Environmental Science International
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• v.33 no.2
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• pp.187-191
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• 2024

This study aimed to investigate the effect of treating environmental purification insect larvae to pig manure on crude ash contents and ammonia production. The experiment set up consisted go two groups: 1 kg of each 3^rd instar TM (Tenebrio molitor) and 3^rd instar PBS (Protaetia brevitarsis seulensis) larvae in Experiment 1 or 3^rd and 4^th instar of HI (Hermetia illucens L.) larvae in Experiment 2 were treated with 5 kg of pig manure. In Experiment 1, the crude ash content was higher in TM larvae-treated pig manure at days 0 and 5 (p>0.05), but was similar to that in PBS larvae-treated pig manure over (p>0.05). Ammonia production was observed at day 0 of TM and PBS larvae-treated pig manure (p<0.05), but did not occur thereafter. For Experiment 2, there was significant difference in crude ash content of 3^th and 4^th instar HI larvae-treated pig manure on day 15. Additionally, ammonia production was found in 3^th and 4^th instar HI larvae-treated pig manure at days 0 and 5, but did not continue over time. In conclusion, treating TM, PBS and HI to pig manure changed the crude ash contents and reduced ammonia through the ability to decompose pig manure. Thus, environmental impact can be minimized using environmental purification insect larvae.