• Tuberculosis and Respiratory Diseases
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• v.56 no.2
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• pp.187-197
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• 2004

Background : Nitric Oxide (NO) is a multi-faceted molecule with dichotomous regulatory roles in many areas of biology. NO can promote apoptosis in some cells, whereas it inhibits apoptosis in other cell types. This study was performed to characterize NO-induced cell death in lung epithelial cells and to investigate the roles of cell death regulators including iron, bcl-2 and p53. Methods : A549 cells were used for lung epithelial cells. SNP (sodium nitroprusside) and SNAP (S-nitroso-N-acetyl- penicillamine) were used for NO donor. Cytoxicity assay was done by MTT assay and crystal violet assay. Apoptotic assay was done by fluorescent microscopy after double staining with propidium iodide and hoecst 33342. Iron inhibition study was done with RBCs and FeSO4. For bcl-2 study, bcl-2 overexpressing cells (A549-bcl-2) were used and for p53 study, Western blot analysis and p53 functionally knock-out cells (A549-E6) were used. Results : SNP and SNAP induced dose-dependent cell death in A549 cells and fluorescent microscopy revealed that SNAP induced apoptosis in low doses but necrosis in high doses while SNP induced exclusively necrotic cell death. Iron inhibition study using RBCs and FeSO4 significantly blocked SNAP-induced cell death. And also SNAP-induced cell death was blocked by bcl-2 overexpression. Finally, we found that SNAP activate p53 by Western blot analysis and that SNAP-induced cell death was decreased in the abscence of p53. Conclusion : In lung epithelial cells, NO can induce cell death, more precisely apoptosis in low doses and necrosis in high doses. And iron, bcl-2, and p53 play important roles in NO-induced cell death.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.12
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• pp.108-116
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• 2009

In this paper, we propose a Ethernet based audio distribution system with a new L1/L2 hybrid switching scheme, and evaluate its performance. The proposed scheme not only offers guaranteed low latency and jitter characteristics that are essentially required for the distribution of high-quality uncompressed audio traffic, and but also provide an efficient transmission of data traffic on the Ethernet environment. The audio distribution system with a proposed scheme consists of a master node and a number of relay nodes, and all nodes are mutually connected as a daisy-chain topology through up and downlinks. The master node generates an audio frame for each cycle of 125us, and the audio frame has 24 time slotted audio channels for carrying stereo 24 channels of 16-bit PCM sampled audio. On receiving the audio frame from its upstream node via the downlink, each intermediate node inserts its audio traffic to the reserved time slot for itself, then relays again to next node through its physical layer(L1) transmission - repeating. After reaching the end node, the audio frame is loopbacked through the uplink. On repeating through the uplink, each node makes a copy of audio slot that node has to receive, then play the audio. When the audio transmission is completed, each node works as a normal L2 switch, thus data frames are switched during the remaining period. For supporting this L1/L2 hybrid switching capability, we insert a glue logic for parsing and multiplexing audio and data frames at MII(Media Independent Interlace) between the physical and data link layers. The proposed scheme can provide a good delay performance and transmission efficiency than legacy Ethernet based audio distribution systems. For verifying the feasibility of the proposed L1/L2 hybrid switching scheme, we use OMNeT++ as a simulation tool with various parameters. From the simulation results, one can find that the proposed scheme can provides outstanding characteristics in terms of both jitter characteristic for audio traffic and transmission efficiency of data traffics.

• Journal of Korean Home Economics Education Association
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• v.25 no.1
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• pp.79-94
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• 2013

In this study, the teaching-learning materials and the plan using persimmon juice dyeing, which is one of the traditional natural dyeing methods of Korea, were developed for the purpose of inspiring further awareness of our traditional clothing life-culture. A questionnaire was designed to make a survey on the educational effect, and t-test and Kruscal-Wallis test were conducted to test the significant differences between pre- and post-lessons. A correlation analysis between culture awareness and its education awareness was also done. These prototypes were applied to high school Technology Home Economics classes under the theme, 'Making living goods with persimmon juice dyed fabrics'. Examining the changes in students' awareness of traditional images, and changes in their interest and attitude towards learning in those classes led to the following results. First, as a result of applying LT cooperative learning in order for an understanding of the formation of clothing life-culture and the process of persimmon juice dyeing, many original handicrafts with modern sense were created. Second, a comparative analysis between pre- and post-lessons on the change in students' degree of awareness of traditional clothing life-culture and its education showed that students' awareness had risen after the class; the data was collected from three separate sample groups which had been clustered according to their initial degree of awareness, i.e. high, middle, and low, and the group that scored the lowest before the class showed the greatest improvement. Third, feedback surveys on 'Making living goods with persimmon juice dyed fabrics' classes returned with scores greater than 'moderate', thus revealing that the students' concern and interest towards traditional clothing life-culture hae been enhanced through the class experience. Their attitude had also become more positive. Fourth, since learners with more awareness of the traditional clothing life-culture also showed more awareness of the need for an education designed to promote the culture, a positive correlation between the two had been found. As explained above, the teaching-learning materials and the plan that had been developed for this study has caused a deeper understanding of the value and significance of the traditional natural dyeing and clothing life-culture, and has therefore brought about an educational effect that powers idea creation.

• Journal of Distribution Science
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• v.9 no.1
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• pp.29-38
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• 2011

Since a distribution market was opened in 1993 after the conclusion of the Uruguay Round (UR), various new businesses emerged in the Korean retail industry, such as convenience shops, supermarkets, specialty stores, non-store marketing methods, and cyber shopping malls, in addition to traditional markets and department stores. Competition among these retail businesses has intensified. According to the National Statistical Office (NSO), the growth rate of the total retail industry has shown a 32% increase in the past 5 years. While department stores, supermarkets, specialty stores, and other non-store retailing venues have shown signs of stagnation at a growth rate of 20-30%, which is similar to the growth rate of the entire retail industry, convenience shops and non-store marketing have shown 60-70% growth over the same period. By comparison, the growth rate of cyber shopping malls has nearly tripled. When applying development aspects of retail businesses through the competition to the retail life cycle, mom-and-pop stores and traditional markets have already entered the decline phase as specialty stores reach their maturity phase and demonstrate their limit of growth. Department stores are now in the latter part of the growth phase, which is still considered to have some growth potential. Big super markets are still in the early part of their growth stage although they were introduced 20 years ago. Meanwhile, retail businesses such as convenience stores, supermarkets, mail order houses, and warehouse stores are entering the middle growth phase and are expected to continue with their quantitative growth. At a time when most retail businesses in Korea are in a state of development or in the full growth stage, what kind of new innovative retailing will appear and develop? Moreover, what growth engine will drive it? This study analyzes the appearance of innovative retailing and its development process by establishing a discussable consumer's choice model through the interlocking mutual behavior of differentiated competitiveness and consumers' choice based on an awareness of this issue. The analysis of the results of this study can be summarized as follows. First, if a new vacuum zone emerges at the retail market level, innovative retailing such as low price/low service or high price/high service will emerge simultaneously. Second, if the number of new businesses in the vacuum zone increases, this will create competitiveness among the businesses, and each retailer will develop raising of level. Third, if a new business that raises the level develops, competition between the new and the existing retailer will occur, and an assimilation process between the existing retailing and the new retailing will be unfolded. Fourth, each retailing will promote distribution innovations in order to break the frontier of the existing distribution technology, and other retailing will follow the innovator. On the basis of an analysis of the abovementioned results, this study presents the following three suggestions. First, responding to the consumer's decision-making process on the attributes of retail shops that promote differentiation in strategies, this study established a consumer's choice model that can be discussed in relation to changes in market share. Second, this study provided an analysis of the emerging and developmental processes of innovative retail businesses using a more precise logical structure on the basis of the consumer's choice model described in this study. Third, the development process of retail businesses discussed in this study presented retailing solutions regarding management aspects on how to compose a strong retail mix that can help retail businesses gain competitive advantages in the market.

• Korean Journal of Cognitive Science
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• v.15 no.4
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• pp.31-47
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• 2004

This study was carried out to examine the development of physical knowledge in children. Eighty children aged 3- to 11-year-old and 16 adults were participated in this study. Participants' knowledge about failing, sliding and sinking/floating objects was investigated to understand what kind of knowledge they had, whether their knowledge was organized as theory and what was the nature of the developmental change in physical knowledge. Results showed that, for falling object task children of all age had correct knowledge about object's falling phenomena. However, there were age differences in children's understanding of the cause of object's falling. As the children's age decreased, the frequency of explanation referring to the absence of supper rather than the gravity as the cause of falling phenomena increased. For the sliding object task, children of all age could predict the motion of sliding object correctly. But only a few 9- and 11-year-old children could understand the effect of object weight and relations between gravity, frictional force and their interactions. Children under age 7 showed no evidence of possessing these knowledge. For sinking or floating object task, children of all age and even adults showed difficulties in understanding the sinking or float phenomena per se. For the cause of these phenomena although a few 9- and 11-year-old children referred to buoyancy as the cause, they had no correct knowledge about the buoyancy. This was also true for the adults. As a conclusion, the results of this study suggested that, not 3, but as young as 5-year-old children's physical knowledge exited as a form of naive theory in terms of their use as a causal devise in explaining the cause of object motion. However, even the theory of 9- and 11-year-old children was lack of the abstractness and coherence, which were also important characteristics of a theory. Finally, developmental change in physical knowledge proceeded toward more frequent and consistent use of physical knowledge as causal device and more abstract and coherently organized theory.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.6
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• pp.708-719
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• 2007

Glutathione S-transferase genotypes GSTT1, GSTM1 and GSTP1 were characterized in 104 healthy male and female subjects and compared with parameters of oxidative stress at the level of DNA and lipids, with antioxidant enzymes, and with plasma antioxidants in smokers and non.smokers. Of the 104 subjects studied, 57.4% were GSTT1 present and 47.6% were GSTM1 present. The GSTP1 polymorphisms a and b were represented as follows: a/a, 75.5%; a/b, 21.6%; b/b type, 2.9%. The GSTT1 null genotype was associated with decreased glutathione in erythrocytes and elevated lymphocytes DNA damage. GST-Px was higher in GSTT1 null compared with GSTT1 present type. The homozygous GSTP1 genotype was not associated with any antioxidant status or DNA damage. The difference in plasma ${\alpha}$-carotene and erythrocytes GSH-Px and GST activities between smokers and non-smokers was detected in the GSTT1 null genotype. Plasma ${\gamma}$-tocopherol and ${\beta}$-carotene decreased significantly in smokers having GSTM1 null genotype. When GSTT1 and GSTM1 were combined, plasma lycopene and erythrocyte GST were reduced in smokers in both null types of these genes. As for GSTP1 genotype, plasma ${\alpha}$-carotene and erythrocytes GSH-Px decreased significantly in smokers with GSTP1 b/b, while erythrocytes GSH-Px activities decreased in smokers with GSTP1 a/b. The different ${\beta}$-carotene level between smokers and non-smokers was seen with both GSTP1 a/a and a/b genotype. It seems that polymorphisms in the phase II metabolizing enzyme glutathione S-transferase may be important determinants of commonly measured biomarkers.

• Korean Journal of Environmental Agriculture
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• v.13 no.1
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• pp.39-46
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• 1994

Two types of sunshine recorders, Jordan and bimetal, were used for measuring the duration of sunshine and percentage of sunshine in Weather Forecast Offices(WFO) and Weather Observation Stations(WOS) in Korea, respectively.These two gauges showed different values in each element observation. To evaluate the solar energy resources by duration and percentage of sunshine, relevant parameter should be adapted to use the two kinds of data for zoning of agricultural climatic area and comparison of regional solar energy distributions. In this respect, the correlation and distribution pattern were found by analyzing data from the two types of sunshine recorders. The results were as follows. The monthly duration of sunshine by the Jordan type was $50{\sim}60$ hours lower than the bimetal type and its value in May was the highest in a year. The percentage of sunshine by the Jordan type was $5{\sim}10%$ lower than the bimetal type. The seasonal difference of sunshine hour data by two types of sunshine recorder became small in winter but large in summer. Standard deviation of monthly duration of sunshine of WFO and WOS was $11{\sim}32$ and $17{\sim}25$ hours and percentage of sunshine was $3{\sim}11$ and $4{\sim}9$ % respectively. The range of deviation in WOS data was smaller than WFO. The highest distribution of duration and percentage of sunshine was in the Southern Coastal Area, whereas the lowest in the Central North Western Area.

• Cartoon and Animation Studies
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• s.32
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• pp.63-90
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• 2013

The purpose of this study is to improve the standards for the animation education in '2011 Arts and Culture Education Standards: Cartoon and Animation'. Animation Education Standards has been developed by discussion about Arts and Culture Education, and by study of documents about animation, and by investigation about experimental recognition. And the study of domestic and foreign art education standards are reflected in these standards. These standards consist of seven categories reflecting various aspects of animation and each category is classified into five stages by age. This standard has two axes: seven categories by various aspects of animation and five stages by age, and it represents achievement requirement in animation learning. This study suggests that 'Animation Education Standards' has five features as below. First, it reflects the character of Animation as an independent subject. Second, it is studied on the basis of the framework of arts and culture education. Third, it provides basic achievement requirement, and it has no limitation in learning activities. Fourth, the achievement requirements of two or more categories can be applied simultaneously in real classroom; each category is set by themes of animation education. This study presents five methods to improve existing standard. First, independent standard for animation education must be developed. Second, categories must be established distinctly. Third, each categories and achievement requirement must reflect unique characteristic of animation. Fourth, the description of achievement requires must be systemized. Fifth, to help to adapt education standard, explanation about practical teaching and learning activities must be added. '2011 Art and Culture Education Standards: Cartoon and Animation' is important in that it is the first study that systemized animation education and presented pedagogical achievement requirements by suggesting achievement requirements for animation education by two axes -ages and categories. It will be very helpful in studying the animation education as a subject, and developing education programs, and teaching and learning in an actual class.

• Korean Journal of Poultry Science
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• v.44 no.2
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• pp.113-122
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• 2017

This study was conducted to investigate the effect of dietary supplementation with Lactobacillus, medicinal plant extracts, and plant extracts on growth performance, cecal microflora, relative organ weight, and lymphocyte profile in broiler chickens. One hundred broilers (5 wk old) were used and divided into five (treatment) ${\times}$ two (challenged Salmonella) groups containing 10 broilers. The treatments were as follows: no antibiotics group (NC), antibiotics group (PC), 0.1% lactic acid bacteria group (LB), 100 ppm medicinal plant extract group (MPE), and 100 ppm herb extract group (HE). A basal diet was formulated as 3,100 kcal/kg ME and 20% CP based on corn and soybean meal. Broilers were fed with the experimental diets with no challenge for 1 wk and with challenge of SG for 2 wk. SG were added in water as $1.0{\times}10^6cfu/L$. Final body weight and weight gain were significantly decreased following challenge with SG (P<0.05). However, with the addition of antibiotic alternatives, growth performance was improved and reduction of performance following challenge with SG was lowed compared with that of the NC treatment (P<0.05). Coliform bacteria and Salmonella but not lactic acid bacteria increased with the addition of antibiotic alternatives (P<0.05). Lactobacillus increased significantly with the addition of lactic acid bacteria compared with the NC and PC treatments (P<0.05). The weight of liver, spleen, and bursa of Fabricius increased with addition of antibiotic alternatives (P<0.05). WBC was highly reduced at 4 days after challenge with SG, but was normally maintained thereafter. There was no significant difference in coliform bacteria, Salmonella, organ weights, or WBC with the addition of antibiotic alternatives. Finally, Lactobacillus, medicinal plant extract, and herb extract can be used as antibiotic alternatives; but one alternative completely can't alternate as antibiotics. Therefore, further studies are needed to investigate the synergistic effects of two or more antibiotic alternatives.

• Food Science of Animal Resources
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• v.27 no.1
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• pp.117-121
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• 2007

The number of internet users and the scale of electronic on-line trading are on the increase due to the development of information technology and the internet. The aim of this study was to monitor the accuracy of country-of-origin labels and the indicated contents of meat available by electronic on-line trading by using a structural interview sheet for 100 on-line meat product markets. The result of this investigation showed a 100% level of accuracy for business name and telephone number whereas the company address, meat manufacturer and supplier, and business registration were less reliable. We also investigated the accuracy of site policy, e-mail address, and fax number. The results showed that the accuracy of fax numbers was the lowest. The product name and the kind of meat actually in the product showed a 100% level of conformity, while the price (96.3%), place of origin (93.6%), capacity (90.4%), meat parts (80.9%) and contents of the product (73.4%) showed a relatively low level of conformity. Serious safety issues were exposed by the disturbingly low 20.2% accuracy of indicated expiration dates and 5.3% accuracy of indicated manufacturing dates. To ensure food safety, it is essential to improve consumer understanding and trust regarding food safety through continuous public relations. More education and information are needed to raise consumer awareness of the facts versus myths regarding food safety.