• The Magazine of the IEIE
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• v.30 no.5
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• pp.551-551
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• 2003

현재의 라우팅 프로토콜은 다양한 사용자 요구를 만족시켜주기 위해서는 네트워크의 처리량을 최대화하고 동시에 사용자의 요구 시 QoS를 보장해주는 기법이 요구되고 있다. 기존의 최단경로 라우팅 프로토콜은 단일경로 라우팅으로 인해 병목현상의 단점을 지니고 있다. 즉, 원천과 목적지간 최단경로는 낮은 활용도를 나타내는 경로들이 많이 존재하지만 단일경로를 선택하므로서 폭주(congestion)의 발생확률이 높다. 최근에 들어 사용자의 QoS 요구 시, 다양한 QoS를 패킷 네트워크에서 처리할 수 있도록 IETF에서 DiffServ, RSVP, MPLS 등과 같은 패킷 QoS 기법에 대한 표준화 작업이 진행중이며, 그 중에서 Diffserv 네트워크가 대표적이다. 따라서 본 논문에서는 이 DiffServ 네트워크상에서 다양하게 유입되는 트래픽의 종류에 따라 사용자의 응용에 적절히 대응하여 트래픽을 처리하는 라우팅 기법 및 알고리즘을 연구하고 기존의 최선형 (Best effort) 트래픽을 처리하기 위한 트래픽 분산 라우팅 프로토콜 (Traffic-Balanced Rout-ing Protocol'TBRP)을 제안하였으며, 최적의 중간 노드를 선택하여 높은 순위의 상호형 데이터를 처리하기 위한 계층적 라우팅 프로토콜(또ierarchicalTra(fic-Scheduling Routing Protocol : HTSRP)을 연구하였다. 본 연구에서 제시한 프로토콜은 유, 무선망의 통합에 따른 다양한 엑세스망과 백본망에 유연한 트래픽 처리기법으로서 계층적 라우팅 알고리즘으로 적합하였다. 본 실험에서는 사용자의 QoS요청 시 제공되는 상호형 또는 스트리 밍 데이터를 위한 HTSRP_Q(Hierarchical Traffic-Scheduling Routing Pro-tocol for QoS)에 대해 성능이 우수함을 입증하였으며, 각 엑세스 단에서 요청하는 QoS 파라미터에 따라 자원을 최적화하여 QoS를 보장하고, 특히 지연에 민감한 트래픽을 처리하였으며, 제안한 프로토콜을 이용하여 사용자 요구 트래픽 종류에 따라 대화형 클래스, 스트리밍 클래스, 높은 순위의 상호형 클래스, 낮은 순위의 상호형 클래스, 그리고 background 클래스등 5개의 서비스 클래스로 분리하여 트래픽 특성에 맞게 처리할 수 있었다. QoS 관련 실험에서는 QoS 요청데이터를 균등하게 1에서 10Mbps 사이에 분포하였고 연결된 호에 대한 지속시간은 5분으로 하였다. 이러한 환경에서 프로토콜을 MaRS에 의해 실험을 하였고 기존의 거리-벡터 라우팅과 링크-상태 라우팅 프로토콜과 비교해서 처리량, 메시지 손실, 블럭킹율 등에서 비교적 우위의 성능을 확인할 수 있었으며, 특히, 차별화된 서비스의 특성에 맞게 라우팅 기법을 적용하므로서 망의 효율성과 안정성을 꾀할 수가 있었다. 연결 수 대 처리량에서는 HTSRP 프로토콜이 연결이 적을 때 DVR, LSR보다 우월하였으며 특히, 선형을 유지하였다. 연결 수 대 패킷 손실에서 HTSRP프로토콜에서 메시지 손실은 연결의 수가 낮거나 높을 때 다른 DVR과 LSR 라우팅 프로토콜과 유사한 결과를 나타내었다. Hotspo에서 TBRP, HTSRP프로토콜은 hotspot 연결의 수가 9일 때까지 DVR, LSR 보다 좋은 처리량를 나타냈고 HTSRP는 연결의 수가 6 이상일 때 가장 높은 처리량을 나타내었다. 일반 트래픽과 QoS 트래픽이 흔재할 경우는 트래픽이 증가할수록 HTSRP_Q가 가장 월등하였으며 , 로드가 증가할수록 낮은 블록킹률을 나타내었다. 본 논문에서는 점대점 전송을 기반으로 하였다. 앞으로 다양한 응용 S/W는 멀티캐스트 기반이 예상되므로 멀티캐스트 라우팅에 대한 연구가 필요하다. 본 논문의 프로토콜은 원천과 목적지간의 최단경로가 폭주상태가 아닌 해당 중간 노드를 이용한다. 최단경로의 모든 링크상의 트래픽 부하가 낮을 때 중간노드의 사용은 지연을 증가시킨다. 향후 최적의 성능을 위해 보완이 필요하다. 아울러, 2계위에서는 일반 트래픽과 QoS 트래픽이 혼재할 때 자동으로 네트워크의 효율적을 고려한 방법 선택이 필요하다.

• KDI Journal of Economic Policy
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• v.26 no.2
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• pp.3-62
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• 2004

This paper estimates Korea's natural rate of unemployment using various estimation methods such as pure time-series methods, reduced-form methods, and structural form methods, with discussion about relative advantages and disadvantages of each estimation method. This paper also provides the confidence interval of the estimated natural rate of unemployment by the Monte Carlo integration method. Though multivariate unobserved component model exhibits better performance in many aspects than other estimation methods, awareness should be raised for a potential misspecification problem of a multivariate unobserved component model. Considering that each method has its own advantages and disadvantages, it is recommended to make an inference on the natural rate of unemployment based on common results among various methods. Korea's natural rate of unemployment was estimated to be around 3.8~4.0% on average in the period of 1979:I~1987:IV, and to decline to 2.5~2.9% in the period of 1988:I~1997:IV. During the Asian crisis, it is estimated to peak at near 4.8% and to have been on a downward trend since then.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.1-10
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• 1999

The dynamic relationships among import prices of roundwood are analyzed using the time series approach. A vector autoregression(VAR) model is estimated for six import prices(New Zealand, Chile, Russia, U.S.A., PNG, and Malaysia). Then Granger's causality test, variance decomposition analysis, and impulse response function analysis are also conducted. The major results are summarized as follows : (1) The prices of New Zealand and Russia are caused by only own lagged prices. (2) The prices of Chile and PNG are effected by New Zealand, the price of PNG is effected by New Zealand and Russia, and the price of U.S.A. is effected by those of Chile and PNG, respectively. (3) An exogenous shock in New Zealand will affect the prices of New Zealand, PNG, U.S.A., Chile, Russia. (4) An exogenous shock in Chile may also affect the prices of Chile, U.S.A., Russia.

• Journal of Soil and Groundwater Environment
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• v.11 no.5
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• pp.43-50
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• 2006

Bioreporter bacteria, such as recombinant bioluminescent bacteria, have been used for the detection of specific compounds in complex environmental media. In this study, optimum conditions for the preparation and application of deep-freezed and Iyophilized recombinant bioluminescent strain KG1206 were investigated for the future application on contaminated environmental sites. Genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, contains TOL plasmid and the plasmid inserted $P_{m}$, promoter on the upper part of lux gone in vector pUCD615, and m-toluate and benzoate are considered direct inducers for bioluminescence. Optimum conditions determined for the preparation and application of the deep-freezed and lyophilized strain were followings: cryoprotective agent (24% sucrose), lyophilization time (12 hrs), strain concentration ($OD_{600}=0.6$), reconstitution for freezed strain (quick reconstitution at $35^{\circ}C$), reconstitution for lyophilized strain ($3{\sim}6$ hrs exposure on LB medium), carrying conditions (keep at $20^{\circ}C$ after reconstitution). These results demonstrate the feasibility of deep-freezed or lyophilized state of genetically engineered bioluminescent strain for environmental usage.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.103-111
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• 2019

DNA methylation is involved in diverse processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, carries out $N^6$-adenine or $N^4$-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Celeribacter marinus IMCC12053 from the Alphaproteobacterial group was isolated from a marine environment. Single molecule real-time sequencing method (SMRT) was used to detect the methylation patterns of C. marinus IMCC12053. Gibbs motif sampler program was used to observe the conversion of adenosine of 5'-GANTC-3' to $N^6$-methyladenosine and conversion of $N^4$-cytosine of 5'-GpC-3' to $N^4$-methylcytosine. Exocyclic DNA methyltransferase from the genome of strain IMCC12053 was chosen using phylogenetic analysis and $N^4$-cytosine methyltransferase was cloned. IPTG inducer was used to confirm the methylation activity of DNA methylase, and cloned into a pQE30 vector using dam-/dcm- E. coli as the expression host. The genomic DNA and the plasmid carrying methylase-encoding sequences were extracted and cleaved with restriction enzymes that were sensitive to methylation, to confirm the methylation activity. These methylases protected the restriction enzyme site once IPTG-induced methylases methylated the chromosome and plasmid, harboring the DNA methylase. In this study, cloned exocyclic DNA methylases were investigated for potential use as a novel type of GpC methylase for molecular biology and epigenetics.

• Radiation Oncology Journal
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• v.19 no.2
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• pp.171-180
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• 2001

Purpose : Expression of TIMP, intrinsic inhibitor of MMP, is regulated by signal transduction in response to genotoxins and is likely to be an important step in metastasis, angiogenesis and wound healing after ionizing radiation. Therefore, we studied radiation mediated TIMP expression and its mechanism in head and neck cancer cell lines. Materials and Methods : Human head and neck cancer cell lines established at Asan Medical Center were used and radiosensitivity $(D_0)$, radiation cytotoxicity and metastatic potential were measured by clonogenic assay, n assay and invasion assay, respectively. The conditioned medium was prepared at 24 hours and 48 hours after 2 Gy and 10 Gy irradiation and expression of TIMP protein was measured by Elisa assay with specific antibodies against human TIMP. hTIMP1 promoter region was cloned and TIMP1 luciferase reporter vector was constructed. The reporter vector was transfected to AMC-HN-1 and -HN-9 cells with or without expression vector Ras, then the cells were exposed to radiation or PMA, PKC activator. EMSA was peformed with oligonucleotide (-59/-53 element and SP1) of TIMP1 promoter. Results : $D_0$ of HN-1, -2, -3, -5 and -9 cell lines were 1.55 Gy, 1.8 Gy, 1.5 Gt, 1.55 Gy and 2.45 Gy respectively. n assay confirmed cell viability, over $94\%$ at 24hrs, 48hrs after 2 Gy irradiation and over 73% after 10 Gy irradiation. Elisa assay confirmed that cells secreted TIMP1, 2 proteins continuously. After 2 Gy irradiation, TIMP2 secretion was decreased at 24hrs in HN-1 and HN-9 cell lines but after 10 Gy irradiation, it was increased in all cell lines. At 48hrs after irradiation, it was increased in HN-1 but decreased in HN-9 cells. But the change in TIMP secretion by RT was mild. The transcription of TIMP1 gene in HN-1 was induced by PMA but in HN-9 cell lines, it was suppressed. Wild type Ras induced the TIMP-1 transcription by 20 fold and 4 fold in HN-1 and HN-9 respectively. The binding activity to -59/-53, AP1 motif was increased by RT, but not to SP1 motif in both cell lines. Conclusions : We observed the difference of expression and activity of TIMPs between radiosensitive and radioresistant cell line and the different signal transduction pathway between in these cell lines may contribute the different radiosensitivity. Further research to investigate the radiation response and its signal pathway of TIMPs is needed.

• Journal of Intelligence and Information Systems
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• v.25 no.1
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• pp.179-196
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• 2019

Recently, the "Smart Consumer" has been emerging. He or she is increasingly inclined to search for and purchase products by taking into account personal judgment or expert reviews rather than by relying on information delivered through manufacturers' advertising. This is especially true when purchasing cosmetics. Because cosmetics act directly on the skin, consumers respond seriously to dangerous chemical elements they contain or to skin problems they may cause. Above all, cosmetics should fit well with the purchaser's skin type. In addition, changes in global cosmetics consumer trends make it necessary to study this field. The desire to find one's own individualized cosmetics is being revealed to consumers around the world and is known as "Finding the Holy Grail." Many consumers show a deep interest in customized cosmetics with the cultural boom known as "K-Beauty" (an aspect of "Han-Ryu"), the growth of personal grooming, and the emergence of "self-culture" that includes "self-beauty" and "self-interior." These trends have led to the explosive popularity of cosmetics made in Korea in the Chinese and Southeast Asian markets. In order to meet the customized cosmetics needs of consumers, cosmetics manufacturers and related companies are responding by concentrating on delivering premium services through the convergence of ICT(Information, Communication and Technology). Despite the evolution of companies' responses regarding market trends toward customized cosmetics, there is no "Intelligent Data Platform" that deals holistically with consumers' skin condition experience and thus attaches emotions to products and services. To find the Holy Grail of customized cosmetics, it is important to acquire and analyze consumer data on what they want in order to address their experiences and emotions. The emotions consumers are addressing when purchasing cosmetics varies by their age, sex, skin type, and specific skin issues and influences what price is considered reasonable. Therefore, it is necessary to classify emotions regarding cosmetics by individual consumer. Because of its importance, consumer emotion analysis has been used for both services and products. Given the trends identified above, we judge that consumer emotion analysis can be used in our study. Therefore, we collected and indexed data on consumers' emotions regarding their cosmetics experiences focusing on consumers' language. We crawled the cosmetics emotion data from SNS (blog and Twitter) according to sales ranking ($1^{st}$ to $99^{th}$), focusing on the ample/serum category. A total of 357 emotional adjectives were collected, and we combined and abstracted similar or duplicate emotional adjectives. We conducted a "Consumer Sentiment Journey" workshop to build a "Consumer Sentiment Dictionary," and this resulted in a total of 76 emotional adjectives regarding cosmetics consumer experience. Using these 76 emotional adjectives, we performed clustering with the Self-Organizing Map (SOM) method. As a result of the analysis, we derived eight final clusters of cosmetics consumer sentiments. Using the vector values of each node for each cluster, the characteristics of each cluster were derived based on the top ten most frequently appearing consumer sentiments. Different characteristics were found in consumer sentiments in each cluster. We also developed a cosmetics experience pattern map. The study results confirmed that recommendation and classification systems that consider consumer emotions and sentiments are needed because each consumer differs in what he or she pursues and prefers. Furthermore, this study reaffirms that the application of emotion and sentiment analysis can be extended to various fields other than cosmetics, and it implies that consumer insights can be derived using these methods. They can be used not only to build a specialized sentiment dictionary using scientific processes and "Design Thinking Methodology," but we also expect that these methods can help us to understand consumers' psychological reactions and cognitive behaviors. If this study is further developed, we believe that it will be able to provide solutions based on consumer experience, and therefore that it can be developed as an aspect of marketing intelligence.