• Proceedings of the KAIS Fall Conference
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• 2005.05a
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• pp.301-302
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• 2005

PAHs의 분해를 위해 Bacillus subtilis 와 Phanerochaete chrysosporium의 두 미생물을 사용하였고, 이들을 부착시킬 담체로 코르크와 톱밥를 선정하여 미생물을 접종시켰다. Phenanthrene 분해의 경우, 반응 초기에는 분해 속도가 매우 빠르지만 반응 12일째 분해 속도가 떨어졌다. 분해 속도는 1)톱밥에 담지한 Bacillus subtilis, 2)코르크에 담지한 Bacillus subtilis, 3)톱밥에 담지한 Phanerochaete chrysosporium, 4)코르크에 담지한 Phanerochaete chrysosporium의 순으로 나타났다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.140-141
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• 2001

젓갈의 제조 공정 중 미생물의 작용은 주로 숙성과정과 완제품의 보관과정에 일어나는 것으로 알려져 있다. 우선 숙성과정은 원료와 함께 부착되어온 미생물이 일차적으로 식염농도에 따라 선택을 받아 적응해 가면서 2차적인 대사산물 생성 및 적자 생존에 의하여 우점종을 형성하여 발효에 관여하게 되며, 젓갈은 그 특성상 멸균과정을 거칠 수 없어 숙성에 관여했던 미생물들이 완제품의 보관 중에도 계속 남아있게 되므로 저장성에 있어 문제가 되고 있다. (중략)

• Journal of Korean Society of Environmental Engineers
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• v.32 no.3
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• pp.227-233
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• 2010

This study examined microbial community structures (MCSs) according to environmental factors through DGGE analysis and comparison in various soils collected from near army bases and gas stations. As a result, the similarities based on DGGE band profiles showed the closer relationship in regional properties than in pollution characteristics, probably due to the degree of weak contamination. The highly contaminated samples with oil revealed low MCS similarities with others in the same region and very low with all the other samples in the other regions. Thus the microbial community structure would more be affected by region-based natural factors than by contamination factors in case of minor pollution. All the dominant culturable bacterial species were involved in firmicutes or high GC Gram+ in a major portion of soil samples and the highly oil-contaminated samples contained Arthrobacter, Bacillus, Methylobacterium, Clavibacter, Streptomyces and Nocardia as reported genera, and Leifsonia as a unreported genus.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.99-112
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• 2020

Rhizoremediation, based on the ecological synergism between plant and rhizosphere microorganisms, is an environmentally friendly method for the remediation of petroleum hydrocarbon-contaminated soils. In order to mitigate global climate change, it is necessary to minimize greenhouse gas emissions while cleaning-up contaminated soils. In rhizoremediation, the main factors affecting pollutant remediation efficiency and greenhouse gas emissions include not only pollutant and soil physicochemical properties, but also plant-microbe interactions, microbial activity, and addition of amendments. This review summarizes the development in rhizoremediation technology for purifying oil-contaminated soils. In addition, the key parameters and strategies required for rhizoremediation to mitigate climate change mediation are discussed.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.374-381
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• 2011

The beneficial effects of Eisenia fetida on soil properties have been attributed to their interaction with soil microorganisms. The bacterial diversity of the intestinal tract of E. fetida was investigated by culture-dependent and culture-independent methods including denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses. In a pure culture, Lysinibacillus fusiformis (51%), Bacillus cereus (30%), Enterobacter aerogenes (21%), and L. sphaericus (15%) were identified as the dominant microorganisms. In the DGGE analyses, B. cereus (15.1%), Enterobacter sp. (13.6%), an uncultured bacterium (13.1%), and B. stearothermophilus (7.8%) were identified as the dominant microorganisms. In the pyrosequencing analyses, Microbacterium soli (26%), B. cereus (10%), M. esteraromaticum (6%), and Frigoribacterium sp. (6%) were identified as the dominant microorganisms. The other strains identified were Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. The results illustrate that culture independent methods are better able to detect unculturable microorganisms and a wider range of species, as opposed to isolation by culture dependent methods.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.09a
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• pp.241-246
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• 2004

매립지 차수층에는 다짐점토층이 사용되고 있지만 건조/수축, 동결/융해 등의 환경적인 영향에 대한 내구성을 향상시키기 위한 기능성 복합차수층에 대한 연구가 진행되고 있다. 본 연구에서는 원지반토양에 고화재를 첨가한 BLT층과 벤토나이트와 미생물 배양액을 첨가한 BLM 층으로 구성된 복합차수층의 차수기능과 BLM층에서 미생물 배양액 첨가에 따른 Bio-barrier 기능을 평가하였다. 원지반토양에 고화재와 벤토나이트를 각각 8, 10%(w/w)를 첨가하였r고, 미생물 배양액은 1%(v/w)을 첨가하였다. 실험결과 원지반토양은 SC계열의 토양으로 분류되었고, BLT, BLM 차수층의 8% 혼합비율에서도 1$\times$ $10^{-7}$cm/sec 보다 낮은 투수계수를 나타내었다. BLM 층에 첨가된 미생물 배양액에 의한 Bio-barrier 기능 실험결과 미생물 배양액 첨가에 의하여 침출수에 대한 내구성이 향상된 것을 확인할 수 있었다.

• Applied Chemistry for Engineering
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• v.4 no.1
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• pp.26-40
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• 1993

Many different types of synthetic surfactant are being used, but it is important to develop for a broad range of purposes in a large variety of different application. Biosurfactant are important because the present a much broader range of surfactant types and properties than the available synthetic surfactant Futhermore, they are easily biodegradable and low toxicity, which reduce the potential of pollution. This article discusses the structure and properties of the glycolipid biosurfactants, their production and isolation from fermentation broths and their potential applications.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.389-396
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• 2023

In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.

• The Microorganisms and Industry
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• v.19 no.3
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• pp.2-11
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• 1993

최근 각 유전자들이 크로닝되고 단백질과 유전자의 특성이 밝혀지면서 PTS를 코드하는 유전자의 발현 조절기작과 PTS 단백질 구조에 대한 정보가 축적되었다. 본고에서는 현재까지 밝혀진 PTS 단백질들의 기능과 성질 및 그 유전자들의 조절작용에 대해 알아보고 PTS가 물질의 대사에 미치는 조절작용은 따로 언급하고자 한다.

• The Microorganisms and Industry
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• v.16 no.3
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• pp.15-19
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• 1990

본 연구에서는 lignin 분해시 lignin peroxidase와 glucose oxidase의 역할을 규명하기 위하여 백색부후균의 일종인 Pleurotus ostreatus를 실험 재료로 하여 glucose oxidase와 세포의 peroxidase를 분리하여 그 특성을 규명하여 이미 분리된 타 균주의 효소와 비교 분석하고, .betha.-O-4 linkage를 지닌 이합체 모델화합물에 작용시켜 그 산물을 분석함으로서 간접적이나마 두 효소의 역할및 분해기작을 추정하여 보았다.