• Title/Summary/Keyword: 미생물 총 균수

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Effect of Salt Concentration on Tongchimi Fermentation (동치미의 발효숙성에 미치는 소금농도의 영향)

  • Moon, Sung-Won;Cho, Dong-Wuk;Park, Wan-Soo;Jang, Myung-Sook
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.11-18
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    • 1995
  • 소금농도가 동치미의 발효숙성중에 미치는 영향을 조사하기 위하여, 동치미 담금액의 소금농도를 달리하여 제조한 동치미를 $4^{\circ}C$에서 85일 까지 저장하면서 pH, 총산함량, 환원당, 소금농도, 비타민 C 및 동치미 국물의 탁도 등의 이화학적 특성과 미생물학적 및 관능적 특성을 조사하였다. 전체적으로 발효가 진행됨에 따라 pH는 점차로 $pH3.93{\sim}pH3.72$까지 낮아지고, 총산함량은 0.18"$0.22%$ 까지 증가함을 보였다. 동치미 국물에서 소금농도는 감소하였지만, 동치미 무에서는 증가되어 발효$15{\sim}22$일에 동치미 무와 액사이의 소금농도가 평형상태에 도달했음을 볼 수 있었고, 이때 동치미 담금액의 초기 소금농도 1.5%, 3.0%, 4.5%, 6.3%는 각각 평형 소금농도 $1.2%{\pm}0.04%$, $2.0%{\pm}0.07%$, $2.8%{\pm}0.05%$, $4.0%{\pm}0.06%$에 도달하였다. 비타민 C와 환원당 함량은 동치미 국물에서 발효 $15{\sim}22$일 까지 점차 증가하다가 그이후로는 서서히 감소되었다. 그러나, 동치미 무에서는 같은 기간 동안에 계속적인 감소를 보였다. 동치미 국물의 탁도는 발효가 시작되면서 증가하여 발효 22일에 모든 시료에서 최대의 탁도를 보이고 그 이후로는 점차로 감소하였다. 총세균수와 젖산균수의 변화는 초기에 총세균수가 조금 많은 것을 제외하고는 발효가 진행됨에 따라 거의 비슷한 변화 양상을 보였는데 발효 $15{\sim}22$일에 젖산균수나 총세균수 모두 최대 균수를 보이고 그 이후로는 모든 시료에서 감소하였다. 관능적 평가에서는 짠맛의 정도와 전반적인 기호도에서 모두 큰 유의차를 보였으며, 특히 소금농도 3.0%의 동치미가 높은 점수를 얻어서 관능적으로 우수한 평가를 받았다.

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Sterilization Effects on Mulberries (Morus alba L.) Washed with Electrolyzed Water and Chlorine Dioxide (전해수와 이산화염소수 세척에 따른 뽕나무 오디(Morus alba L.)의 살균효과)

  • Teng, Hui;Lee, Sun-Ho;Lee, Won-Young
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.5
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    • pp.654-661
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    • 2013
  • The current research is designed to analyze sterilization effects on mulberries in terms of storage temperature and storage period after treating with tap water (TW), electrolyzed water (EW) and aqueous chlorine dioxide ($ClO_2$). The treated $ClO_2$ concentrations are 10, 50, 100 and 200 ppm. In each concentration, the mulberries are soaked for 30 seconds respectively. The sterilization effects are being compared at room temperature ($25^{\circ}C$) and at $4^{\circ}C$, respectively. And the enzyme activity related to quality is also being investigated and analyzed about for browning inhibition effects. Microbial sterilizing power increases more in treating plots with EW and $ClO_2$ than treating plot with TW. Futhermore sterilizing power of $ClO_2$ increased sharply on high concentration treatment plot as well. Sterilization effects of $ClO_2$ during storage time are better at cold temperature. Pictures taken from scanning electron microscope reveal that there are no microbes in sterilizing solutions treatment plots. From measurement of the enzyme activity, it is concluded that activities decrease more in sterilizing solutions treatment plots as comparing with TW treated plot during the time. The amount of total polyphenolics decrease with the time passing and EW and $ClO_2$ treatment shows less contents than TW treatment. Thus, EW and $ClO_2$ treatment of mulberris are considered as method to improve safety by reducing total plate count and to contribute to quality maintenance and to extend storage time.

Physiochemical and Microbiological Changes of the Fermented Dandelion (Taraxacum officinale) Extracts with Raw Sugar (당 첨가 민들레(Taraxacum officinale) 발효 추출물의 발효과정 중 이화학적 및 미생물학적 변화)

  • Kim, Kyung-Min;Kim, Young-Nam;Choi, Byoung-Kon;Oh, Deog-Hwan
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.131-137
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    • 2012
  • This study was carried out to investigate the physiochemical and microbiological changes of dandelion during fermentation. Thirty and fifty percentage raw sugar groups (SFE30 and SFE50) were introduced into dandelions and fermented for 120 days at $15-20^{\circ}C$. This study was conducted to investigate the utilization of sucrose on lactic acid bacteria from dandelions and their effect on the pH, titratable acidity, microorganism and formation of organic acids in dandelions during fermentation. The number of lactic acid bacteria increased remarkably up to 15 days of fermentation and then decreased rapidly thereafter. The maximum number of lactic acid bacteria, 7.9 log CFU/mL was reached at pH 4.17 and the pH of dandelion showed a slight decrease during fermentation and decreased steadily up to 90 days to reach an optimum pH of 4.0. The titratable acidity of dandelions fermented increased during fermentation. The concentration of organic acid, amino acid and free sugar in SFE30 was higher than both SFE50 and DWE. The results of dandelions fermented were remarkably retarded in the 50% raw sugar group compared to the 30% raw sugar group.

Enhanced antimicrobial effectiveness of Omija (Schizamdra chinesis Baillon) by ClO2 (chlorine dioxide) treatment (오미자 수확 후 이산화염소수를 이용한 표면 세척에 따른 미생물 저감 효과)

  • Lee, Seul;Moon, Hey-Kyung;Lee, Su-Won;Moon, Jae-Nam;Lee, Sun-Ho;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.20 no.6
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    • pp.871-876
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    • 2013
  • The purpose of this study was to investigate antimicrobial effectiveness of Omija (Schizandra chinensis Baillon) treated with $ClO_2$ (chlorine dioxide) concentration (10, 15, 20, 25 and 30 ppm), washing time (30, 60 and 90 sec) and multiple proportion (x1, x2, x3 and x4). The seven groups were divided into control (Omija without washing water treatment), W-T (Omija treated by tap water ($20{\pm}1^{\circ}C$) for 30 seconds), $ClO_2$-10 (Omija treated by 10 ppm $ClO_2$), $ClO_2$-15 (Omija treated by 15 ppm $ClO_2$), $ClO_2$-20 (Omija treated by 20 ppm $ClO_2$), $ClO_2$-25 (Omija treated by 25 ppm $ClO_2$), $ClO_2$-30 (Omija treated by 30 ppm $ClO_2$), and then they were detected number of total aerobic bacteria, yeast and mold. The rate of inactivation was found, for microorganisms of total aerobic bacteria, yeast and mold, to increase with a increase of $ClO_2$ treatment concentration and multiple proportion. No total aerobic bacteria, yeast and mold in $ClO_2$-30 sample treated for 30 sec, $ClO_2$-15 treated for 60 sec and $ClO_2$-10 treated for 90 sec were detected, and in $ClO_2$-30 Omija with multiple proportion ${\times}1$ (Omija : 30 ppm $ClO_2$ solution ratio was 1:1 (w/w)), $ClO_2$-20 with ${\times}2$ (Omija : 20 ppm $ClO_2$ solution ratio was 1:2 (w/w)) and $ClO_2$-15 with ${\times}4$ (Omija : 15 ppm $ClO_2$ solution ratio was 1:4 (w/w)) respectively.

Effect of Dandelion (Taraxacum Officinale) Extracts on the Intestinal Microorganisms of Streptozotocin-Induced Diabetic Rats (민들레추출물이 당뇨유발 흰쥐의 장내 미생물 균총에 미치는 영향)

  • 박지윤;장주연;이미경;박은미;김명주;손동화;정현채;조수열
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.6
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    • pp.1112-1118
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    • 2002
  • This study was designed to test the effect of dandelion (Taraxacum officinale) extracts on the intestinal microorganisms of streptozotocin-induced diabetic rats. Male Wistar rats were divided into normal, diabetic control and dandelion extract groups. The extracts were prepared by water, ethylacetate and ether from leaf and root, respectively. Diabetes was induced by injecting streptozotocin (55 mg/kg BW, i.p.) in citrate buffer. The extract was supplemented in 11.45g of raw dandelion / kg diet for 50 days. The growth of Lactobacillus was more enhanced in dandelion leaf-water and leaf-ethylacetate extract group than that of the diabetic control group, whereas the growth of E. coli decreased. Results indicate that the dandelion extracts would be effective to improve the intestinal microorganisms.

Physicochemical and Microbiological Properties of Dongchimi added with Citron (Citrus junos) (유자 첨가 동치미의 이화학적 및 미생물학적 특성)

  • 장명숙;김나영
    • Korean journal of food and cookery science
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    • v.13 no.3
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    • pp.286-292
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    • 1997
  • The purpose of this study was to investigate the effect of citron (Citrus junos) on Dongchimi (watery radish kimchi) fermentation. Dongchimi with various levels (0, 1, 2, 4, 6%) of citron was fermented at 10$^{\circ}C$ for 36 days. The pH of Dongchimi decreased slowly in all samples during fermentation, however, it was slightly lower in citron-added Dongchimi. Total acidity and turbidity of Dongchimi increased gradually during formentation, in which the level of increase was greater as the % of added citron increased. However, the total acidity and turbidity of Dongchimt without citron increased dramatically on 36$\^{th}$/ day of fermentation, and reached the values similar to those of Dongchimi with 6% citron. Reducing sugar content increased gradually up to 15∼23 days of fermentation and decreased rapidly for the rest of the period. The content of vitamin C was much higher in citron-added Dongchimi, and especially it was 4 times higher than that of without citron at the beginning of fermentation. The number of lactic acid bacteria reached the maximum on 15$\^{th}$/ day in citron-added Dongchimi and on 30$\^{th}$/ day in Dongchimi without citron, and decreased thereafter.

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Quality Characteristics of Basil Pesto added with Various Nuts during Storage (견과류를 달리하여 제조한 바질 페스토의 저장 중의 품질특성)

  • Park, Jong-Woo;Kim, Ki-Bbeum;Choi, Soo-Keun
    • Culinary science and hospitality research
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    • v.22 no.2
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    • pp.29-43
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    • 2016
  • Pesto were prepared with five different nuts including pine nut, sunflower seed, pumpkin seed, walnut and almond to examine the antioxidant properties(total polyphenols, total flavonoids, electron donating ability) and sensory test(attribute difference, acceptance). Pesto were measured based on color value, pH, viscosity, total cell numbers for 0, 5, 10, 15 and 20 days at $4^{\circ}C$. The higher total phenol and total flavonoid content of pesto added with various nuts was higher antioxidant capacity. Basil pesto added with sunflower seeds and walnuts were considered to be more functional more than pine nuts addition. The attribute difference test results was the color intensity, nut taste, bitter taste, coarseness, oily taste, thickness were evaluated significant difference kinds of nuts. Gloss, basil flavor, nuts flavor, basil taste were not evaluated significant difference in kind of nuts. The preference test results reveal that the appearance, taste, texture and overall preference level was the highest in PNP(pinenut pesto) and SSP(sunflower seed). L value, pH, decrease while a value, b value and viscosity increased as kind of nuts in during storage. Total cell number increased and then decreased a little at a certain point. When prepared pesto, SSP(sunflower seed) was the highest in antioxidant property, and change of color value, pH, viscosity, total cell number were the lowest. In addition to the use of pine nuts, sunflower seeds have been identified are possible.

Effect of kimchi on the Microbiological properties of fermented sausages during ripening period (김치 첨가가 발효소세지 숙성중 미생물 특성에 미치는 영향)

  • 한영실;김순임;정해옥;전희정;백재은
    • Korean journal of food and cookery science
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    • v.17 no.3
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    • pp.224-228
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    • 2001
  • We studied the effects that the addition of Kimchi has on microbiological properties of fermented sausages as we observed pH and microbiological changes of Kimchi-added fermented sausage by aging period. The pH figure of sausages added with commercial starter culture was 5.69 at the beginning stage and declined gradually to 5.01. The more Kimchi is added, the lower pH figure goes. Sausages with 10% and 20% of fresh Kimchi, and 2% and 4% of powdered Kimchi added, showed about pH 5.4 at the early stage and down to pH 4.87 as they are stored longer. In terms of microbiological changes, sausages mixed with starter culture showed 10$\^$7/cfu/g level in the number of total bacteria, lactic acid bacteria, yeast and fungi, lower than Kimchi-added sausages. However, the figure soared sharply to 10$\^$10/cfu/g during the aging period. Kimchi-added sausages recorded 10$\^$9/cfu/g, slightly high during the eurly aging stage, in the number of total bacteria, lactic acid bacteria, yeast and fungi, while showing a gradual increase up to 10$\^$10/cfu/g towards the end of aging period and became microbiologically stabilized.

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Effect of Prunus mume Extract on Kimchi Fermentation (매실농축액이 김치 발효 중 품질에 미치는 영향)

  • Park, Hee-Juhn;Choi, Moo-Young
    • Korean Journal of Plant Resources
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    • v.20 no.4
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    • pp.342-347
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    • 2007
  • The present study was conducted to examine the effect of prunus mume extract on the Kimchi fermentation. The physicochemical and microbiological properties of Kimchi with the addition of various levels(0, 0.1, 0.3 or 0.5%)of prunus mume extract was measured up to 30 days at $4^{\circ}C$. pH was decreased sharply in control after 2 days . A typical decreasing pattern in pH values was noted in all treatments during the initial 18 days of fermentation followed by a gradual decrease afterwards. Total acidity was decreased by the addition of prunus mume extract. Reducing sugar content was reduced to approximately 70% by day 10-12 in prunus mume extract treatments. Total vitamin C content was reached to maximum on 6 days of fermentation and then decreased in all treatments. Reducing sugar and total vitamin C contents were slightly increased by the addition of prunus mume. Total viable cell number increased to reach peak on day 8 and then slowly decreased during the fermentation. Lactobacillus cell number in control treatment continued to increase to reach peak on day 12, while the numbers in prunus mume treatments reached peak on day 8-12 and then gradually decreased throughout the fermentation. The results of this study indicate that prunus mume extract causes to delay the Kimchi fermentation by slowing down pH drop and inhibiting the Lactobacillus cell growth.

Fermentation Patterns of Leek Kimchi and Chinese Cabbage Kimchi (부추김치와 배추김치 발효양상)

  • 안순철;김태강;이헌주;오윤정;이정숙
    • Korean Journal of Microbiology
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    • v.37 no.3
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    • pp.234-238
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    • 2001
  • For the comparison of fermentation pattern of leek kimci with chinese cabbage kimchi, the changes of total viable cell number, Leuconostoc sp. bacteria, Lactobacillus sp. bacteria, pH and total sugar content of twotypes kimchies were investigated during fermentation at $20^{\circ}C$ and $10^{\circ}C$. In chinese cabbage kimchi at $20^{\circ}C$ fermentaion, the numbers of total viable cell, Leuconostoc sp. bacteria and Lactobacillus sp. bacteria reachedthe maximum level on 2nd day and reduced slowly. But in leek kimchi, the maximum numbers of total via-ble cells, Leuconostoc sp. bacteria and Lactobacillus sp. bacteria were obtained after 3 days fermentation,and the cell number of Lactobacillus sp. maintained at the maximum level oyer 15 days. At $10^{\circ}C$ fer-mentation, in both kimchies, the viable cell number of lactic acid bacteria more slowly increased anddecreased than at $20^{\circ}C$. The pH of chinese cabbage kimchi was 4.2 on 3rd day (optimal ripening phase) andmere decreased to 3.5 after 5 days, but in leek kimci the pH 4.2 could be reached after 10 days at $20^{\circ}C$. At $10^{\circ}C$, the optimal ripening pH 4.2 of chinese cabbage kimchi was reached after 6 days, but in leek kimchieven though after 24 days, the pH was maintained oyer 4.3. The total sugar contents of chinese cabbage him-chi and leek kimci were decreased continuously during fermentation. From these results, we know that thefermentation of leek kimchi proceed more slowly than chinese cabbage kimchi by the retardation of lacticacid bacteria growing in leek kimchi.

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