• Title/Summary/Keyword: 미생물 제제

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Isolation of Bacillus amyloliquefaciens MJ-3 and Its Effect on the Early Growth Promotion of Red Pepper Plug Seedlings in Compost (고추 플러그묘 초기 생육을 촉진시키는 Bacillus amyloliquefaciens MJ-3의 분리 및 상토내 처리 효과)

  • 김진호;최용화;강상재;주길재;서장선;임태헌
    • Journal of Life Science
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    • v.13 no.5
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    • pp.582-589
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    • 2003
  • The effect of useful rhizobacterium added in bed soil on the early growth promotion of red pepper plug seedlings was investigated. Total 540 colonies of rhizobacteria from 385 samples of eggplant family roots were isolated. Among these, 5 isolates were selected for antifungal activity against pathogenic fungi such as Alternaria solani, Botrytis cinerea, Fusarium oxysporium, Phytophthora capsici, and Sclerotia sclerotiorum. Of all the isolates, MJ-3 having the most pronounced growth-promoting ability for red pepper was finally selected and identified as Bacillus amyloliquefaciens through characterization of biochemical and bacteriological aspects and 16S rDNA sequence. The plant height, stem diameter, root length and fresh weight of red pepper plants which were grown with inoculation of B. amyloliquefaciens MJ-3 were higher than those without inoculation. Especially the root weight of the inoculated red pepper plant increased by 44.3%, the content of endogenous plant hormone (CA$_1$) being 0.556 ng/g (dry weight).

Isolation of Microorganisms and Development of Microbial Augmentation for Treatment of Industrial Wastewater containing Ammonium Nitrogen (암모니아성 질소함유 산업폐수처리를 위한 미생물의 분리 및 복합미생물제제의 개발)

  • Lee, Myoung-Eun;Mun, Seo-Jin;Kwon, Do-Hyuck;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.30 no.2
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    • pp.129-136
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    • 2020
  • For effective treatment of wastewater containing ammonium nitrogen (NH4-N), AT2, AT9, and AT12 strains, having high total organic carbon (TOC) removal capability, and FN47, possessing excellent ammonia nitrogen removal capability present in the activated sludge in the aeration tank of food wastewater treatment plants, were isolated and identified. The cells of these isolated strains were used for microbial augmentation with FIW-1 in the defatted rice bran as a medium to treat industrial wastewater. The investigation of the cultural characteristics of these isolated strains in the aeration tank showed that the affinities for substrate of the isolated strains were extremely high, of which AT12 (Alcaligenes sp. AT12) was the highest among the isolated strains. Ammonium nitrogen removal efficiency in the food wastewater was 71% in the isolated strain FN47 (Microbacterium sp. FN47) treatment group. When only activated sludge was added in the lab scale pilot using food wastewater during continuous culture experiment, the TOC removal efficiency was 63%. Meanwhile, the removal efficiency of 92% was obtained when the microbial augmentation FIW-1 for wastewater treatment was applied. In addition, the chemical oxygen demand (COD) level from the effluent wherein microbial augmentation FIW-1 was input for the initial three days in the wastewater treatment site experiment showed a treatment rate of about 43%, which was increased to 62% after an elapse of 5 days.

Biological Control of Pythium Root Rot by Radiation Induced Mutant, Bacillus lentimorbus WJ5a17 (방사선유도 돌연변이체 Bacillus lentimorbus WJ5a17에 의한 Pythium Root rot의 방제)

  • 이영근;김재성;장병일;장유신;이호용
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.276-285
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    • 2003
  • To control pythium root rot, Bacillus lentimorbus WJ5a17 with high anti-fungal activity against Pythium ultimum was induced from B. lentimorbus WJ5 by gamma radiation ($^{60}Co$). The biocontrollers of FWJ5 and FWJ5a17 were formulated ($1.0\times 10^{11}$) with B. lentimorbus WJ5 and WJ5a17, respectively, The population density of FWJ5 and FWJ5a17 maintained highly up to $1.0\times 10^{9}$ CFU $g^{-1}$ in nursery and field soils until 30 days after treatment. P. ultimum spores germination were inhibited 71.0% and 81.4% by FWJ5 and FWJ5a17, respectively. Pythium root rot of yea pepper, Chinese cabbage and radish were significantly (p < 0.05) controled by one time treatment of FWJ5 and FWJ5a17.

Development of a Biocontrol Agent Using Monacrosporium thaumasium to Control a Root Knot Nematode, Meloidogyne incognita (Monacrosporium thaumasium을 이용한 뿌리혹선충 (Meloidogyne incognita) 방제용 미생물제제의 개발)

  • Choi, Ye-Hoon;Kim, Keun-Ki;Son, Hong-Joo;Shin, Hae-Soo;Park, Hyean-Cheal
    • Journal of Life Science
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    • v.17 no.12
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    • pp.1605-1609
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    • 2007
  • The created microbial pesticide was used to examine its effects using the inside-pot test method. The selected microbial pesticide KBC3017 particle pesticide manufactured by using Monacrosporium thaumasium was used in the farm-house outdoor test to find the optimum consistency and its effects. The more amount used, the better effect it showed. However, the optimum consistency was 2% and the KBC3017 particle pesticide for which the diatomite and raw jade powder were used as an increaser, when used 2% level of the total amount of soil, showed 71% effect on nematode prevention. The root and the stem of crops were better compared to those without any pesticide used.

Advanced Treatment of Shipboard Sewage by RCM Process with BM (복합미생물제제를 이용한 RCM공법의 선박오수 처리장치 적용에 관한 연구)

  • Ha, Shin-Young;Kim, In-Soo
    • Journal of Navigation and Port Research
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    • v.38 no.5
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    • pp.451-456
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    • 2014
  • Lab scale experiment was carried out to study biological wastewater treatment technology developed for shipboard. RCM process using BM(Beneficial Microorganisms) was investigated for practical application on shipboard sewage treatment. RCM process is an environmental friendly treatment system, with minimum production of sludge. In the test, BOD5, CODcr, T-N and T-P were reduced a 96%, 97%, 78% and 81.68% respectively. From the result it was suggested that RCM process with BM might be a suitable process for shipboard sewage treatment, maintenance of useful microorganisms and creating special environment as the SDC tank is circulating in the non-biodegradable organics sludge generated during the wastewater treatment, thus reducing the amount of sludge generated. Therefore, the RCM process does not require additional equipment to strengthen it to meet the marine pollution standards.

Loosing the Constipation by Capsulated Yogurt (캡슐 발효유에 의한 변비 개선)

  • 안태석
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.94-97
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    • 1999
  • Evaluation of capsulated yogurt for bowels was carried out. To the twenty volunteers who has selfconsciousness of constipation, thc two bottles of capsulated yogurt were applied daily for two weeks. By the results, totally 90% of volunteers replied that their constipation was loosing during the application and 95% after the 2 weeks of stopping the application.

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유류오염 방제에 생물정화제제 형식승인제도 도입

  • Yun, Ju-Yong
    • The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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    • v.37 no.5
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    • pp.22-28
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    • 2008
  • 바다에 유출된 기름으로 오염된 지역의 친환경적인 방제를 위하여 기름분해 미생물을 이용하는 생물정화기술을 소개하고, 해양환경관리법 시행('08.1.21)으로 방제약제에 새로 추가된 생물정화제제의 성능시험기준 및 검정기준 등 형식승인절차를 기술하였다.

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A Field Research on Mud Flat Remediation by Biological Treatments (생물학적 처리에 따른 갯벌 복원을 위한 현장 적용성 연구)

  • Cho, Dae-Chul;Bae, Hwan-Jin;Kwon, Sung-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.7
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    • pp.3285-3294
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    • 2012
  • A field test on mud flat remediation was carried out in order to observe the effects of the treatments such as microbial dose and an oxygen releasing compound like $CaO_2$. The size of each treatment site was $100m^2$ and the dosage was 3.6 kg per site. The 6 week monitoring showed that pH on two sites was below 7 and ORP increased from .178~-188 mV to .121~-142 mV. In Ignition loss and COD there were no significant changes. Meanwhile nitrogen and phosphorus concentrations changed: ammonia concentration decreased both on control and treatment sites. Nitrate nitrogen decreased more on combined treatment site than on single microbial treatment (11.3% vs. 7.3%) probably because the extra oxygen supplied by $CaO_2$ formed more oxic environment so that the facilitated nitrification might produce more nitrate but the nitrate would be much rapidly released into the water layer out of the sediment. That also explains the total nitrogen reduction(6.1%). Similarly, T-P and $PO_4-P$ reduced by 29% and 31.8%, respectively on combined treatment sites, resulting from the phosphorus release effect though the initial concentrations of the two factors were considerably high.

Isolation of Microorganisms and Development of Microbial Augmentation for Treatment of Paper Mill Wastewater (제지폐수 처리용 미생물의 분리 및 복합 미생물제제의 개발)

  • Kang, Dae-Ook;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.21 no.4
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    • pp.554-560
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    • 2011
  • This study was performed to investigate the effects of microbial augmentation on the biological treatment of paper mill wastewater. Three bacteria (KN11, KN13, KN27) capable of degrading aromatic compounds and a bacterial strain (GT21) producing an extracellular cellulase were isolated from soil and wastewater by selective enrichment culture. Through morphological, physiological, and biochemical taxonomies, isolated strains of KN11, KN13, KN27, and GT21 were identified as Acinetobacter sp., Neisseria sp., Bacillus sp., and Pseudomonas sp. and named Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, respectively. For analysis of non-biodegradable and chemical oxygen demand (COD)-increasing matter in a paper mill wastewater, we utilized GC/MS to detect aromatic compounds and their derivatives containing several substituted functional groups. The microbial augmentation, J30 formulated with the mixture of bacteria including Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, was used for the treatment of paper mill wastewater. The optimum temperature and pH for COD removal of the microbial augmentation, J30, were $30^{\circ}C$ and 7.5, respectively. For evaluation of the industrial applicability of the microbial augmentation, J30 in the pilot test, treatment efficiency was examined using paper mill wastewater. The microbial augmentation, J30, showed a COD removal rate of 87%. On the basis of the above results, we designed the wastewater treatment process of the activated sludge system.