• Title/Summary/Keyword: 미생물 오염

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For Refuse of marine dredged eco-friendly cleaning technology (미세 해양오염퇴적물의 생물학적 처리기술 개발)

  • Kim, In-Soo;Ha, Shin-Young;Jeong, Kyung-Chul
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2012.06a
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    • pp.167-168
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    • 2012
  • 본 연구는 해양퇴적물 준설 중 해양의 탁도와 2차오염을 유발하는 미세한 입자의 퇴적물을 생물학적으로 처리하는 친환경 정화기술로 유용미생물제제(BM-S-1)를 투여한 Lab Scale의 실험장치를 이용하여 기초 실험을 수행하였다. 유용미생물제제(BM-S-1)가 우점되어 있는 Lab Scale 실험장치를 운전하여 유기물 정량분석방법인 COD, T-N, T-P를 분석해본 결과 모든 항목이 약 98% 이상 처리됨을 확인할 수 있었다. 특히 본 실험대상물질인 해양퇴적물은 고농도의 염분이 함유되어 있어 기존기술만으로는 생물학적 처리가 어려웠지만, 본 연구에서 사용된 유용미생물제제(BM-S-1)은 염분이 함유된 오염 퇴적물에서도 효과적인 생물학적 처리가 가능함을 확인 할 수 있었다. 따라서 준설 시 2차오염을 유발시키는 미세한 입자의 해양퇴적물을 본 공법으로 처리하여 방류할 시 친환경적인 준설이 이루어질 수 있으며 이 때 처리되어 배출되는 미세토양은 재이용 가능하다고 판단된다.

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The Soil Properties and Microbial Numbers of soil Samples Collected from Polluted and Unpolluted Areas in Korea (오염지역과 비오염지역의 토양의 특성과 토양 미생물의 분포)

  • 심재욱;이민순;이상선;이태수;이민웅
    • Journal of Korea Soil Environment Society
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    • v.3 no.2
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    • pp.31-39
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    • 1998
  • A total of 112 soil samples collected from polluted and unpolluted areas in Korea were investigated for physical properities (such as soil moisture, organic matter and soil pH) and biological properties (such as microbial numbers). The results of organic matter and soil pH showed a great variation(p=0.01) in the four areas, whereas soil moisture and organic matter were similar among three plant vegetations. There was a significant relationship(p=0.01 or 0.05) between soil pH and microbial number These results imply some variations in soil environment and may lead to unfavorable changes of plant vegetation in soil. Presumably, the above results appear to be resulted from soil acidification caused by an acidic rain.

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먹는 생물과 미생물학적 품질

  • Cha, Seong-Gwan
    • Bulletin of Food Technology
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    • v.8 no.2
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    • pp.20-26
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    • 1995
  • 건강한 성인 한사람은 하루에 약 $3\ell$의 물을 외부로부터 섭취하여야 한다고 한다. 이중 약 $1-2\ell$의 물은 음료 즉, 먹는물의 형태로 외부로부터 섭취하게 되는데, 이러한 음료의 형태로 섭취되는 먹는 물이 우리의 건강을 좌우하게 된다는, 물은 많이 마실수록 건강에 좋고, 깨끗한 생수를 마시는 것이 건강의 비결이라는 말을 많이 듣게 된다. 또 환경오염으로 인한 식수의 오염문제가 심각하게 대두되고 있어 많은 사람들은 오염되지 않은 깨끗한 물, 생수 등을 찾고 있는 실정이다. 이러한 한국의 현실에서 1995. 5. 1. 부터 환경부의 "먹는물 관리법"이 새롭게 시행되기 시작하여 지금까지 사용되어오던 "광천음료수"가 "먹는샘물"이라는 용어로 변경이 되고 몇가지 품질규격에 있어 더욱 엄격하게 규제가 되기 시작하였다. 따라서 새롭게 시작되는 "먹는물 관리법"의 "먹는샘물"의 정의와 먹는샘물의 미생물학적인 품질의 설명이 필요하고, 또 "먹는물", "먹는샘물"이 환경쪽에서만 다루어야 할 문제가 아니라 먹는 물은 항상 식품과 함께 취급되기 때문에 식품연구의 일환으로서도 취급되어져야 함을 강조하고자 이글을 쓰게 되었다.

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Microflora of Manufacturing Process and Final Products of Saengshik (시판생식의 제조공정 및 최종제품의 미생물분포)

  • Chang, Tae-Eun;Moon, Sung-Yang;Lee, Kun-Wook;Park, Jang-Mi;Han, Jeong-Su;Song, Ok-Ja;Shin, Il-Shik
    • Korean Journal of Food Science and Technology
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    • v.36 no.3
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    • pp.501-506
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    • 2004
  • Microflora and contamination process of Saengshik products were investigated to ensure microbial safety of Saengshik. Food-borne pathogenic bacteria, such as Staphylococcus aureus, Bacillus cereus, and Clostridium perfringens detected mainly from grains were not removed by washing with tap water and freeze-drying. Contaminations of food-borne pathogenic bacteria occurred through raw material powder processed at other factories and during actual product manufacturing process, because detection rates of final products were higher than those of raw materials. Concentration of food-borne pathogenic bacteria increased with advancing of process after first pulverization. Dusts of powder and powder attached to machine were good media for air-borne microorganisms and caused to increase of food-borne pathogenic bacteria during process. Improvement of manufacturing process and sanitary control of machines arc necessary to ensure microbial safety of Saengshik.

Sanitation Effect of Sprouts by Chlorine Water. (염소수처리 의한 새싹채소의 살균 효과)

  • Lee, Kyung-A;Lee, Young-A;Park, In-Shik
    • Journal of Life Science
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    • v.19 no.6
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    • pp.751-755
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    • 2009
  • This study was attempted to provide basic data for effective sanitization of sprouts. Sanitization treatments were performed by dipping four sprouts (alfalfa, broccoli, clover and red radish) into chlorine water. Microbial analyses were composed of the total plate count (TPC), coliform count, and E. coli count. All examined sprouts exhibited high levels of TPC ($10^{7}$ CFU/g) and coliform ($10^{6}$ CFU/g). E. coli was detected in broccoli and red radish sprouts in the range of $10^3{\sim}10^4$ CFU/g. Among chlorine water sanitization, the microbial reduction was largest in 100 ppm chlorine water, and its TPC and coliform counts decreased to $8.0{\times}10^5{\sim}2.7{\times}10^6$ CFU/g and $4.3{\times}10^5{\sim}4.6{\times}10^5$ CFU/g, respectively. E. coli was not detected in all sprouts that were given 100 ppm chlorine water treatment. The effective dipping time in 100 ppm chlorine water treatment was 30 min and 60 min, in which TPC were below the microbiological safety limits of ${\times}10^{6}$ CFU/g. Coliform counts were decreased to $9.1{\times}10^4{\sim}2.4{\times}10^5$ CFU/g when the sprouts were dipped for 30min, and kept the similar level after that time. These levels exceeded the microbiological safety limits of $10^{3}$ CFU/g. E. coli was not detected in samples by 100 ppm chlorine water treatment.

Study on Geochemical Behavior of Heavy Metals by Indigenous Bacteria in Contaminated Soil and Sediment (국내 일부 오염 토양 및 퇴적물 내 토착 미생물에 의한 중금속의 지구화학적 거동 연구)

  • Song, Dae-Sung;Lee, Jong-Un;Ko, Il-Won;Kim, Kyoung-Woong
    • Economic and Environmental Geology
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    • v.40 no.5
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    • pp.575-585
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    • 2007
  • Microbial control of the geochemical behavior of heavy metals (Cd, Cu, Pb, and Zn) and As in contaminated subsurface soil and sediment was investigated through activation of indigenous bacteria with lactate under anaerobic condition for 25 days. The results indicated that dissolved Cd, Pb and Zn were microbially removed from solutions, which was likely due to the formation of metal sulfides after reduction of sulfate by indigenous sulfate-reducing bacteria. Soils from the Dukeum mine containing a large amount of sulfate resulted in complete removal of dissolved As after 25 days by microbial activities, while there were gradual increases in dissolved As concentration in soils from the Hwabuk mine and sediments from the Dongducheon industrial area which showed low $SO_4{^2-}$ concentrations. Addition of appropriate carbon sources and sulfate to contaminated geological media may lead to activation of indigenous bacteria and thus in situ stabilization of the heavy metals; however, potential of As release into solution after the amendment should be preferentially investigated.

바이오 에너지

  • 이상기
    • The Microorganisms and Industry
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    • v.14 no.3
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    • pp.33-36
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    • 1988
  • 대체에너지를 개발하고자 할때 반드시 고려햐야 할 사항으로는 새롭게 개발된 대체에너지가 산업의 석유의존도를 낮출 수 았어야 하고, 환경을 오염시키지 않으며, 공급이 안정되고 필요에 따라서는 언제라도 재생이 가능해야 한다. 이러한 제 조건을 충족시킬 수 있는 대체에너지로서 가장 유망한 것이 무한한 태양에너지를 생물학적 전환방법을 통해 연료화할 수 있는 바이오에너지이다. 바이오에너지란 광합성을 통해 형성된 바이오매스를 생물학적인 시스템을 이용하여 여러가지의 에너지형태로 전환시킨 것이다. 바이오매스의 생물학적 전환과정에서 가장 중요한 역할을 담당하는 것이 생태계에 널리 분포하고 있는 미생물이며 이들 미생물의 독특한 물질대사와 환경조건에 따라 다양한 형태의 바이오에너지가 얻어지는 것이다.

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Rapid Microbiological Assessment Method by using ATP-Bioluminescence in Ginseng Powder (ATP-Bioluminescence를 이용한 인삼분말의 미생물 신속검사법)

  • 곽이성;김천석;송용범;고셩룡
    • Journal of Ginseng Research
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    • v.25 no.3
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    • pp.127-129
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    • 2001
  • Bioluminescence technique was applied to ginseng powders. ATP bioluminescence can be used as a rapid method that can be implemented for microbiological monitoring of contaminated ginseng powders. The RLU (relative light units) of ATP was proportion to bacterial CFU (colony forming units) when in high contaminated ginseng powders ($\geq$ about 1.0$\times$10$^4$CFU/g). However, when in low contaminated ginseng powders ($\times$10$^4$CFU/g), the RLU was not proportion to CFU, respectively.

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The Microbiological Assessment and Identification of Food Utensils and Food Service Facilities in School (학교 급식설비 및 집기류의 미생물학적 위해요소 분석)

  • Hong, Seung-Hee
    • Journal of Food Hygiene and Safety
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    • v.29 no.3
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    • pp.189-194
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    • 2014
  • This study was conducted to evaluate microorganism contamination of food utensils and service facilities in school and to prevent hazards by food poisoning occurrence. As a result, the highest number of microorganism growth plate ($12.3{\pm}2.6$) was detected in total bacteria test plate, and also observed $10.3{\pm}3.9$ growth plates in Staphylococcus aureus test plate and $9.5{\pm}3.9$ growth plates in E. coli and coliform bacteria test plate. But we could detect to the lowest number of growth plates ($1.5{\pm}1.0$) in Vibrio test plate. We also assessed that floors were appeared to the highest microorganism contamination rate in food utensils and service facilities. Therefore, $4.5{\pm}0.6$ growth plates was detected in pre-operation floor and $4.3{\pm}1.0$ growth plates in floor. And high level of microorganism contamination also observed in tables as $3.3{\pm}1.0$ growth plates in cooking table and $3.0{\pm}0.0$ growth plates in dining table. The level of microorganism contamination of food utensils such as kitchen knife, cutting board, and food tray were lower than that in food service facilities. We analysed microorganism contamination according to purpose of use in kitchen knifes and cutting boards. The microorganism contamination rate in fish kitchen knife ($2.0{\pm}0.8$) and fish cutting board ($1.3{\pm}1.5$) were slightly higher than that of others purpose of use. As a result of microorganism identification, various strains of microorganism were contaminated in food service facilities and some strains could detected more than two times. Especially, Staphylococcus aureus was repeatedly identified in cooking table, trench, and kitchen knife. Bacillus cereus was identified in kitchen knife, and then Pseudomonas fluorescens and Pseudomonas aeruginosa were also detected in food utensils and service facilities as known to food spoilage microorganisms. Klebsiella pneumoniae was detected four times repeat, which widely distribute natural environment as normal bacterial flora but sometimes cause acute pneumonia. These results suggest that food utensils and service facilities are contaminated with not only major food poisoning microorganisms such as Staphylococcus aureus, but also food spoilage microorganisms. Taken together, strict personal hygiene control and efficient food service facilities management will be needed to enhance food safety in school feeding and to improve student health.

유류오염토양의 화학적 고도산화 현장적용기술 개발

  • 김국진;고일원;이광표;이철효
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.235-238
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    • 2004
  • 유류 오염토양에 대한 과산화수소의 화학적 고도산화에 대한 실험실 및 현장 적용 가능성을 타진하였다. 현장 오염토양 10g에 각각 과산화수소(34.5%) 원액을 중량비로 0.3%, 0.6%, 1.0%, 2.0%를 처리하여 혼합 후 실험실에서 1일 반응 후 TPH 농도를 분석하였다. 분석결과 토양 대비 2.0%의 과산화수소를 투입한 처리구에서 가장 높은 복원 효율을 측정할 수 있었고 2.0%까지의 투입농도에서 미생물의 농도 변화는 측정되지 않았다. 오염된 토양에 과산화수소를 오염 토양 중량 대비 2%를 분무기로 살포 후 로타리가 설치된 경작 장비로 뒤집기 수행하여 1일 후 오염토양의 오염농도를 분석한 결과 초기 TPH 5,365ppm의 농도가 2,317ppm으로 56.8% 복원된 것을 확인할 수 있었다.

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