• Magazine of the Korean Society of Agricultural Engineers
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• v.53 no.1
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• pp.27-40
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• 2011

토양정보는 식량생산, 지속적인 토지이용 계획, 종다양성 평가에 사용되는 기본적인 자료이다. 우리나라 토양조사의 역사, 다양한 축척의 토양도 구축과 토양검정, 토양도와 토양검정 자료의 특성, 농업환경변동 모니터링을 통한 일반농경지 및 취약농경지 토양, 토양정보의 전산화에 따른 토양데이터베이스와 토양정보시스템 소개, 구축된 토양정보의 활용과 향후 방향에 대해 논하였다. 40여년 동안 수행되었던 국책 토양조사사업 결과 두 종류의 토양 데이터베이스가 구축되었는데, 다양한 축척의 토양도(1:250,000, 1:50,000, 1:25,000, 1:5,000)를 GIS DB로 전산화한 수치토양도 DB와 필지 단위로 조사된 화학성 위주의 토양분석 성적을 구축한 토양비옥도 DB이다. 흙토람(http://soil.rda.go.kr)에 서 제공하는 토양전자지도는 총 111종으로 토성, 경사, 지형, 모재, 배수등급, 자갈함량, 유효토심 등 토양 GIS 주제도 50종, 사과, 배, 마늘, 수박 등 작물 재배적지 61종이고, 82종의 토양통계 정보를 제공하고 있다. 최근 에는 친환경농업육성법 시행령에 따른 경작형태 및 오염원별 농경지 토양의 이화학성 및 농업용수 수질 조사 자료를 GIS DB로 구축하여 공간적인 분포와 시계열적인 변화를 분석하는 자료로 활용하고 있다. 농업환경변동 정보는 농업환경자원 인벤토리를 기반으로 일반농경지의 화학성과 농업용수의 수질, 토양 미생물 등의 공간적인 분포와 시간적인 변화 정보를 전문가 그룹에 제공하고 있다. 앞으로는 자연자원의 분포와 변동 정보를 바탕으로 보다 일반적이고 알기 쉽게 가공하여 일반인들에게 농업환경과 자연자원 보전의 중요성을 이해시키는 정보로 제공할 예정이다. 기존의 토양정보와 농업환경정보를 통합하여 컨텐츠를 보다 내실있게 하고 정보 수요자별로 손쉽게 목적에 맞는 접근을 할 수 있는 시스템을 만들어 사용자의 편의성을 강화시키는 방향으로 이끌어 가고자 한다.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.9
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• pp.1020-1026
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• 2007

The control of pathogenic microorganisms is important issue in human environments, especially in surface area. However, surface disinfection has not been fully researched. In this study, the surface disinfection under UV irradiation was performed to investigate the quantitative kinetics for Bacillus subtilis spore inactivation in several experimental conditions, such as light intensity, temperature and surface roughness. This study reports that UV light would apparently inactivates the microorganisms and the required IT value for 2 log (99%) Bacillus subtilis spore inactivation was found to be 14.5 $mJ/cm^2$ in plain surface, as predicted by the Delayed Chick-Watson model. When UV was irradiated, there were no significant temperature effects. However, the experimental result shows that the more increased IT values are required at larger surface roughness.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.2
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• pp.139-143
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• 2009

We examined the vertical distribution of specific microbial groups and the patterns of microbial community structure within the soil profile using phospholipid fatty acid (PLFA). Samples were collected from the soil surface down to 15 cm in depth from paddy and upland fields located in Daegu, Korea. The two fields have been fertilized with only chemical fertilizers N, P, K for 33 years. Principal component analysis of the PLFA signatures indicated that the composition of the soil microbial communities changed significantly with the cultivation practices and soil depth, suggesting that cultivation practices of paddy and upland fields had more significant influence on soil microbial community than the soil depth did. The soil microbial communities changed more drastically with soil depth in upland field than in paddy field, with making thicker soil surface in paddy field in terms of soil microbial community. The ratios of cyclopropyl/monoenoic precursors and total saturated/total monounsaturated fatty acids increased with soil depth, suggesting that the deeper soil horizons are more carbon-limited and anaerobic than surface soil. The community analysis using PLFAs as biomarkers revealed that Gram-positive bacteria and actinomycetes tended to increase in proportional abundance with increasing soil depth, while the abundance of Gram-negative bacteria and fungi were highest at the soil surface and substantially lower in the subsurface.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.414-417
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• 2003

유역의 경사가 비교적 급하고, 소규모 축산농가의 미처리된 축산폐수 및 농경지 배수, 산재된 마을하수 등 비점오염원의 유출이 강우시 집중적으로 이루어지는 중산간 농업용 저수지의 수질개선을 위한 공법으로 부영양화된 충남 아산 마산저수지의 유입부에 조성한 저류지를 이용한 수질개선효과 및 물질분해자로서 미생물학적 특성을 2000년부터 2002년까지 3년간 연구함으로서 향후 유사한 농업용저수지의 수질개선 방안으로 실용화 가능성을 모색하고자 하였다. 그 결과 수질정화효과는 총질소와 총인 등 영양염류의 정화효과가 비교적 우수하게 나타났으며, 유기오염물질로서 BOD, COD, SS 등은 평균 수질정화효과는 (-)의 값을 보여주었으나, 강우기에는 정화효율이 높게 나타났다 또한, 저류지의 물질 침전기능 외 오염물질의 활발한 분해작용을 살펴보고자 세균 분포 특성을 조사한 결과 유입수와 저류지, 저수지 각각의 수체에서 조사된 총세균수 및 총세균수에 대한 진정세균수의 비율이 유입수<저류지<유출수의 순으로 나타나 저류지의 유기물질 분해 활성이 높음을 보여주고 있다. 따라서 저수지 유입부에 조성한 저류지의 수질개선효과는 강우시 입자성 유기물질의 효과적 제거 뿐만아니라 영양염류의 제거효과, 특히 질소의 제거효과가 높은 정화기구로서 설치비용이 적고, 유지관리가 간단한 특성을 살려 유사한 중규모 농업용저수지의 수질개선 기법으로 널리 활용할 가치가 있음으로 사료된다.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.153-155
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• 2006

We constructed the null mutants of fission yeast Schizosaccharomyces pombe spSac3 gene that is homologous to budding yeast Saccharomyces cerevisiae SAC3 involved in mRNA export out of nucleus. Tetrad analysis showed that the spSac3 is essential for vegetative growth. The spSac3 mutants harboring pREP81X-spSac3 plasmid showed poly(A)+ RNA export defect in the presence of thiamine. These results suggest that spSac3 is also involved in mRNA export from the nucleus.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.293-301
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• 1998

Taxonomical studies of soil-borne fungi were performed. Soil samples were collected from greenhouses located in Jinju-City, Korea. Soil dilution plating method was adopted for the pure isolation. Seven ascomycetous fungi were isolated from the soil samples, and subsequently identified. Among them, five isolates, Chaetomium brasiliense, C. indicum, Corynascus sepedonium, Petriella sordida, and Thielavia fragilis have never been reported in Korea. Both teleomorphic and anamorphic states were observed in the two species, Corynascus sepedonium and Petriella sordida, and only teleomorphic state was observed in Thielavia fragilis, Chaetomium indicum, and Chaetomium brasiliense. Illustrated descriptions are presented for the isolates examined in the present study.

• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.199-204
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• 2019

This study aimed to generate a probability distribution model based on temperature data of frozen food storage facility as input variables for microbial risk assessment (MRA). We visited 8 food-handling businesses to collect temperature data from their cold storage warehouses. The overall mean temperature inside the storage facilities was $-20.48{\pm}3.08^{\circ}C$, with 20.4% of the facilities having above $-18^{\circ}C$, with minimum and maximum temperature values of -10.3 and $-25.80^{\circ}C$ respectively. Temperature distributions by space locations of natural and forced convection were $-22.57{\pm}0.84$ and $-17.81{\pm}1.47^{\circ}C$, $-22.49{\pm}1.05$ and $-17.94{\pm}1.44^{\circ}C$, and $-22.68{\pm}1.03$ and $-18.08{\pm}1.42^{\circ}C$ in the upper (2.4~4 m), middle (1.5~2.4 m), and lower (0.7~1.5 m) shelves, respectively. Probability distributions from the temperature data were obtained using the program @RISK. Statistical ranking was determined using goodness of fit to determine the probability distribution model. Our results show that a log-normal distribution [5.9731, 3.3483, shift (-26.4281)] is most appropriate for relative MRA conduction.

• Korean Journal of Microbiology
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• v.54 no.2
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• pp.98-104
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• 2018

Naegleria fowleri and Acanthamoeba spp. are free-living amoebas that are widely distributed in natural environments. Although uncommon, infection with these protozoans can cause fatal disease in humans and animals. In this study, in order to select the appropriate method to survey Naegleria fowleri and Acanthamoeba spp. in water samples, four molecular biology techniques and one commercially available kit for real-time PCR were compared. The results indicated that the duplex real-time PCR was the most sensitive, and could be used to simultaneously detect two different free-living amoebas. Using the duplex real-time PCR approach, the two free-living amoebas were surveyed in three local streams in Daejeon, Republic of Korea. The concentrated free-living amoebas were inoculated onto non-nutrient agar plates which had been spread with heat-inactivated Escherichia coli and incubated for 5~7 days. After incubation, gDNA was extracted and used as the template for amplification by duplex real-time PCR. Acanthamoeba spp. and N. fowleri was detected from ten (83.3%) and two (16.6%) of the twelve samples, respectively. As these two free-living amoebas can be fatal, continuous surveillance is needed to track their distribution in the aquatic environment for the drinking water safety.

• Korean Journal of Agricultural Science
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• v.26 no.1
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• pp.77-83
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• 1999

The populations of microorganisms adhered on rice straws which had been ricked in the fields around Chungchong areas during the winter season from February to March in 1998 were investigated. The number of mesophilic bacteria including bacilli was generally high in the middle part of straw. And it appeared to be higher in the samples from rural areas than those from suburb. Thermophilic bacteria and actinomycetes were rarely to be detected in most samples. Coliform bacteria were detected in a few samples which collected from Kwanpyong-dong of Taejon-city, Jangpyong-meun and Jungsan-meun of Chongyang-koon, indicating that these areas were contaminated by sewage and livestock wastes. Following the fermentation of Chungkukgang employed the middle parts of straws from Mok-meun of Chongyang-koon as a source of microbial inoculum, qualities of the fermented products such as amino-nitrogen content, viscosity and protease activity, were examined: the application of rice straws resulted in better qualities of the fermented products when compared to the control, however the methods of application appeared to have little or no effect on the quality.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.501-506
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• 2004

Microflora and contamination process of Saengshik products were investigated to ensure microbial safety of Saengshik. Food-borne pathogenic bacteria, such as Staphylococcus aureus, Bacillus cereus, and Clostridium perfringens detected mainly from grains were not removed by washing with tap water and freeze-drying. Contaminations of food-borne pathogenic bacteria occurred through raw material powder processed at other factories and during actual product manufacturing process, because detection rates of final products were higher than those of raw materials. Concentration of food-borne pathogenic bacteria increased with advancing of process after first pulverization. Dusts of powder and powder attached to machine were good media for air-borne microorganisms and caused to increase of food-borne pathogenic bacteria during process. Improvement of manufacturing process and sanitary control of machines arc necessary to ensure microbial safety of Saengshik.