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Construction of spSac3 Null Mutants Defective in mRNA Export  

Kang Sook-Hee (Department of Biology, and Institute of Basic Sciences, Sungshin Women's University)
Yoon Jin-Ho (Department of Biology, and Institute of Basic Sciences, Sungshin Women's University)
Publication Information
Korean Journal of Microbiology / v.42, no.2, 2006 , pp. 153-155 More about this Journal
Abstract
We constructed the null mutants of fission yeast Schizosaccharomyces pombe spSac3 gene that is homologous to budding yeast Saccharomyces cerevisiae SAC3 involved in mRNA export out of nucleus. Tetrad analysis showed that the spSac3 is essential for vegetative growth. The spSac3 mutants harboring pREP81X-spSac3 plasmid showed poly(A)+ RNA export defect in the presence of thiamine. These results suggest that spSac3 is also involved in mRNA export from the nucleus.
Keywords
deletion mutant; mRNA export; Schizosaccharomyces pombe; spSac3;
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