• Title/Summary/Keyword: 미생물상의 변화

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Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran (쌀겨와 밀기울의 토양 혐기발효 처리가 시설 재배지 토양의 미생물상에 미치는 영향)

  • Kim, Hong-Lim;Weon, Hang-Yeon;Sohn, Bo-Kyun;Choi, Young-Hah;Kwack, Young-Bum
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.341-347
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    • 2009
  • Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20 days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.

Effect of Chitosan, Wood Vinegar and EM on Microorganisms in Soil and Early Growth of Tomato (키토산, 목초액 및 EM 처리가 토양 미생물상의 변화 및 토마토의 초기생육에 미치는 영향)

  • Jeong, Soon-Jae;Oh, Ju-Sung;Seok, Woon-Young;Kim, Jeong-Han;Kim, Doh-Hoon;Chung, Won-Bok
    • Korean Journal of Organic Agriculture
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    • v.14 no.4
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    • pp.433-443
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    • 2006
  • With treatment of Kitosan, Wood vinegear and EM(effective microoganism) which farmers call it as substance in fertilizing, conditioning and disease control substances, authors in vestigated on microorganisms change in soil and ealy growth characteristics of tomato. The results were summarized as follows: Among foliar application of kitosan, wood vinegear and EM(effective microoganism) treatments diluted by chitosan 500 times solution level was effective considering growth of tomato as compared other dilutions and control plot. Change of microorganism number in the soil for cultivation of tomato was increased with microorganism treatment plot as compared with control plot. Specially chitosan 500 times solution level showes the most significant effect.

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Studies on the Microflora and Enzyme Activity in Processing of Accelerated Low Salt-Fermented Anchovy by adding koji (코오지를 이용한 속성 저식염 멸치젓의 미생물상과 효소활성)

  • 백승화;임미선;김동한
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.392-397
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    • 1996
  • To produce low salt fermented anchovy by an accelerated method with Asp. oryzae and Bacillus sp. koji, enzyme activity and variation of microflora during the 60 day fermentation were examined. Bacterial counts changed a little during the fermentation with the highest on day 40 for proteolytic and anaerobic bacteria and on day 20 for aerobic bacteria. Proteolytic, lipolytic, aerobic, and anaerobic bacteria counts were higher in the Bacillus sp. koji added anchovy paste than in others. The protease and lipase activities reached the highest point on day 20 and 30, respectively, and decreased gradually afterwards. The protease activity was higher in Asp. oryzae koji than in bacillus sp. koji, but the lipase activity was to the contrary.

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Changes of microflora in processing and preservation of dried persimmon (곶감의 제조 및 저장 중 미생물상의 변화)

  • 홍은영;김영찬;이창호;강우원;최종욱
    • Food Science and Preservation
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    • v.8 no.4
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    • pp.374-378
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    • 2001
  • We investigated the change of microfloral states during drying processing and storage of dried persimmon. Initial stave of drying, a total bacteria counts of dried persimmon which was made the rural region of Sangju province were higher than that of downtown but after 7 weeks, there was no differents. A total cell count of bacteria decreased during drying processing but mold and yeast increased. In a storage test of dried persimmon at a different temprature, 3$0^{\circ}C$, room temp. 4$^{\circ}C$, respectively, low temprature storage was effectvie not only to retard the growth of microorganism but also maintain the suitable appearence.

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Effects of Organic Amendments on Soil Microbial Community in Red Pepper Field (시용 유기물의 종류가 고추 재배지 토양 미생물상에 미치는 영향)

  • Park, Kee-Choon;Kim, Yeong-Suk;Kwon, Oh-Hoon;Kwon, Tae-Ryong;Park, Sang-Gu
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.2
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    • pp.118-125
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    • 2008
  • Diverse organic amendments available in local areas have been used to improve soil quality in red pepper field and so the need for investigating the soil chemical and biological properties changed by the organic amendments application is increasing. Soil microbial diversities were measured by phospholipid fatty acid (PLFA) and Biolog $EcoPlate^{TM}$. Compost was most effective for improving soil chemical properties including pH, EC, total nitrogen, P, K, and Ca, and bark increased soil organic matter significantly (P=0.05). Compost increased the fatty acids indicating actinomycetes and vascular arbuscular fungi, and ratio of cy19:0/18:1w7c and monounsaturated fatty acids/saturated fatty acids in soils in PLFA analysis. Bark increased soil fungal indicators in PLFA analysis (P=0.05). Principal component analysis of Biolog EcoPlate data and PLFA differentiated the compost- and bark-amended soils from other organic matteramended soils especially the soil incorporated with compost. More researches are needed to use bark for improving soil microbial properties because the soil chemical and microbiological properties caused by compost and bark are significantly different.

동해안 까나리를 이용한 속성 어간장의 유리아미노산 및 미생물상의 변화

  • 최근표;김상무
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.10a
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    • pp.99-100
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    • 2000
  • 어간장은 어패류를 자체의 자가소화 효소 및 미생물이 분비하는 소화효소에 의해서 제조되는 전통적인 수산발효 조미료로서, 최근 일부 회사에서 액젓 및 진국간장 형태로 상품화하고 있다. 또한 식염농도를 낮추고 제조기간을 단축하기 위한 방법으로서 단백질 가수분해 효소를 이용한 속성 제조를 가장 많이 사용하고 있으며, 이와 관련하여 제조방법, 조건 및 제품의 품질 형가에 관해서 많은 연구가 수행되어 왔다. (중략)

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저염 창란젓갈 제조 과정 중 미생물상의 변화

  • 윤지혜;이명숙
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.140-141
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    • 2001
  • 젓갈의 제조 공정 중 미생물의 작용은 주로 숙성과정과 완제품의 보관과정에 일어나는 것으로 알려져 있다. 우선 숙성과정은 원료와 함께 부착되어온 미생물이 일차적으로 식염농도에 따라 선택을 받아 적응해 가면서 2차적인 대사산물 생성 및 적자 생존에 의하여 우점종을 형성하여 발효에 관여하게 되며, 젓갈은 그 특성상 멸균과정을 거칠 수 없어 숙성에 관여했던 미생물들이 완제품의 보관 중에도 계속 남아있게 되므로 저장성에 있어 문제가 되고 있다. (중략)

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Changes in Chemical Properties and Microbial Population of Farm-Made Organic Liquid Fertilizer during Fermenting Process (농가 자가제조 액비의 발효과정 중 이화학성 및 미생물상 변화)

  • An, Nan-Hee;Kim, Yong-Ki;Lee, Yeon;Jee, Hyeong-Jin;Park, Jong-Ho;Hong, Sung-Jun;Han, Eun-Jung
    • Korean Journal of Organic Agriculture
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    • v.19 no.3
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    • pp.417-425
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    • 2011
  • This study was conducted to investigate the changes in physicochemical and microbiological properties during fermenting process of farm-made organic liquid fertilizer made of the mixture of organic materials such as blood meal and molasse during fermenting process. The pH level of organic liquid fertilizer during the ermentation decreased from 7.2 to 4.3. The EC of organic liquid fertilizer was increased from 13.9 dS/m to 99.3 dS/m during the fermentation. The total population of aerobic bacteria decreased from $8.2{\times}10^5$ cfu/ml to $3{\times}10^4$ cfu/ml, but Bacillus spp. increased from $2.1{\times}10^2$ cfu/ml to $4.2{\times}10^3$ cfu/ml during the fermentation. Bacterial isolates were obtained from organic liquid fertilizers and identified by fatty acid-base typing. The Genus Bacillus was dominant as fermenting proceeded. The denaturing gradient gel electrophoresis (DGGE) profile showed changes of bacterial communities in organic liquid fertilizers.

Seasonal Changes of Microflora in Paddy Soil with Long-term Application of Organic Matter (유기물(有機物) 연용답토양(連用畓土壤)에 있어서 미생물상(微生物相)의 계절적(季節的) 변화(變化))

  • Lee, Sang-Bok;Choi, Yoon-Hee;Lee, Kyung-Bo;Yoo, Chul-Hyun;Rhee, Gyeong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.4
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    • pp.356-362
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    • 1995
  • This experiment was carried out to investigate the effects on the seasonal population change of microflora of long-term application of organic matters in Fluvio-Alluvial plain of Jeonbug series. As organic matters, rice straw and compost of 5 and 10ton/ha, which were applied with the different nitrogen fertilizer level of 0, 150kg/ha into the soil 15cm deep, respectively. A number of total aerobic bacteria were gradually increased from just after water-logging before rice transplanting to pancle formations stage, afterthat decreased at harvest. The other side, a number of actinomycetes, fungi and cellulose-decomposers were slightly fluctuated until panicle formation stage and increased at havesting stage. In general, microorganism numbers were higher in organic matter with long-term nitrogen fertilizer applied plot, while cellulose-decomposers were higher in only organic matter applied plot. The microorganisms of ammonia-oxidizing, nitrate-reducing and nitrite-oxidizing, and denitrifying bacteria showed the maximum number at harvest stage, at panicle formation stage and at early tillering stage, respectively, while that of ammonifying bacteria were variable if nitrogen fertilizer applied or not at the respective periods in nitrogen cycle under water-logging. These bacteria were numerous in the organic matter plots combined with nitrogen fertilizer, especially, denitrifying bacteria in rice straw, others no difference.

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Effects of Organic Materials, Chitosan, Wood Vinegar, and EM Active Solution on Soil Microbial and Growth in Chinese Cabbage (유기농자재인 키토산, 목초액 및 EM 활성액의 처리에 따른 배추의 생육과 토양 미생물상에 미치는 영향)

  • Jeong, Soon-Jae;Cho, Mi-Yong;Seok, Woon-Young;Lee, Sang-Lok;Lee, Hyoung-Joo;Oh, Ju-Sung
    • Journal of Life Science
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    • v.21 no.4
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    • pp.584-588
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    • 2011
  • This study was conducted to investigate the effects of organic materials, chitosan, wood vinegar, and EM active solution, on soil microbial and growth in Chinese cabbage. The organic materials were treated with chitosan, wood vinegar, and EM (effective microoganism) active solution, and the treatment concentration was 100 times solution and 1,000 times solution level with foliar application. The results are summarized as follows: Among foliar application of organic materials treatment plot[?], with 1,000 times solution level of chitosan was effective in inhibiting microbial growth in Chinese cabbage compared to other plots and control. The microorganism number in the soil for cultivation of Chinese cabbage increased with organic materials treatment plot as compared with control. Especially, 1,000 times solution level of chitosan showed the most significant effect.