• Title/Summary/Keyword: 미드시스템

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Plasmid Stabilitly of a Recombinant Escherichia coli and Optimization of Fermentation Processes (유전자 재조합 균주에서 플라스미드의 안정성과 발효 공정의 최적화)

  • 박성훈
    • Journal of Life Science
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    • v.2 no.1
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    • pp.26-34
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    • 1992
  • 유전자 재조합 균주의 발효에 있어서 플라스미드의 안정성과 발효공정의 최적화에 대해 개략적으로 서술하였다. 클론된 DNA의 발현은 플라스미드의 안정성을 크게 저해하며, 저하된 플라스미드의 안정성은 재조합 균주의 생산성을 많이 떨어뜨린다. 최적 발효 조건은 각각의 숙주-벡터 시스템, 사용한 배지, 생성물 등에 따라 크게 변한다. 동일한 숙주-벡터 시스템의 경우도, 사용하는 배지에 따라 온도, 희석률 또는 성장속도에 의존하는 정도가 달라지고 또 최적값도 다 변한다. 또한 발효조건의 최적화가 균체 내 플라스미드의 자기복제,mRNA로의 전사, 단백질로의 translation, 더 나아가 미생물 전체의 생리와 밀접하게 관련이 있다.

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A Measurement Study of Midamble based Cannel Estimation in IEEE 802.11p WAVE System (IEEE 802.11p WAVE 시스템에서 미드엠블을 이용한 채널추정 기법의 측정)

  • Cho, Woong
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.5
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    • pp.733-738
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    • 2013
  • Orthogonal Frequency Division Multiplexing (OFDM) based IEEE 802.11 a/g systems which are widely used in wireless LAN carry out channel estimation in one time per packet since the systems use only preamble. Whereas, midamble based channel estimation supports continuous channel estimation by tracking the channel state information periodically. Using IEEE 802.11p Wireless Access in Vehicular Environments (WAVE) system, we analyze the performance of the proposed system via practical measurements. Based on these results, practical issues on midamble based channel estimation are investigated.

Channel Equalization Schemes using Midamble for WAVE Systems (WAVE 시스템에서 미드엠블을 이용한 채널 등화 방식)

  • Hong, Dae-Ki;Kang, Bub-Joo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.10
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    • pp.2215-2222
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    • 2010
  • A Wireless Access for Vehicular Environment (WAVE) system based on Orthogonal Frequency Division Multiplexing (OFDM) is made for vehicle to vehicle wireless communications. The physical layer standard of the WAVE system is very similar to that of the IEEE802.11a wireless local area network (WLAN). Therefore, the performance of the WAVE system is degraded by continual channel variation in the WAVE multipath fading channels after starting initial channel estimation. In this paper, we research the performance improvement of equalization in 64 Quadrature Amplitude Modulation (QAM) transmission in WAVE environment. The proposed algorithms use the training sequence and the midamble sequence which is used for fast channel variation such as WAVE environments. Additionally, various interpolation methods are also used for the channel tracking.

Preparation and Water Vapor Barrier Properties of PET/Nanohybrid PI Films (폴리에스테르/폴리이미드 나노복합필름의 제조 및 수분차단 특성)

  • Han, Seung San;Kim, Yong Seok;Won, Jong Chan;Lee, Jae Heung;Choi, Kil-Yeong
    • Journal of Adhesion and Interface
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    • v.5 no.1
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    • pp.29-35
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    • 2004
  • We have prepared polyster/nanQhybridized polyimide films in the range of 1~9 wt% of organophilic synthetic layered silicate (STN). Firstly, poly(amic acid)/STN nanocomposite solutions were prepared via solution blending method in DMAc or THF/MeOH solution, and then cast on the polyester film followed by imidization reaction, thermal and chemical method repestively. XRD and TEM experiment showed that the STN was fully exfoliated through the polyimide matrix. Surface morphologies of nanohybridized polyimide films were characterized by AFM and thermal, mechanical properties were also confirmed by TGA, DMA and UTM each. And also, the water vapor permeabilities highly depended on the content of STN. The sample from chemical imidization route and THF/MeOH solvent system showed better water vapor barrier properties than thermal one and DMAc system.

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Analysis of acceleration and deceleration of female hockey players using GPS (GPS를 활용한 여자 하키 선수들의 가속과 감속 분석)

  • Park, Jong-Chul;Kim, Ji-Eung;Choi, Eun-Young
    • Journal of Digital Convergence
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    • v.18 no.6
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    • pp.495-500
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    • 2020
  • This study was intended to quantify the acceleration and deceleration information measured in the competition by using GPS device for the national women's field hockey players and to identify the difference in movement by classifying them by position. For this study, 308 acceleration and deceleration data were collected from 16 women's field hockey players measured at international competitions. According to research results, the frequency of acceleration was in the order of midfield > defender > striker, acceleration distance was midfield > striker > defender. The frequency of deceleration appeared in the order of midfield > defender > striker, deceleration distance was defender > striker > defender. Based on the results of this study, can be used as basic data when setting the strength and amount of training for field hockey players.

Development of Selectable Vector Plasmid in Bacteriophage P2-P4 System and Its Stability (박테리오파지 P2-P4 시스템을 위한 벡터 플라스미드 개발과 안정성)

  • Kim, Kyoung-Jin
    • Korean Journal of Microbiology
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    • v.34 no.4
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    • pp.236-242
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    • 1998
  • While bacteriophage P2-P4 system is very useful experimental tool for the study of viral capsid assembly, there is no useful plasmid vector for the DNA manipulation in bacteriophage P2-P4 system. In this study, a new vector plasmid, P4 ash8 (sid71) kmr, was constructed by swapping the non-essential region of P4 DNA for kanamycin resistance(kmr) gene cassette of plasmid pUC4-K. P4 ash8 sid71 was starting material for the construction, since it tends to be maintained as a plasmid in the absence of the helper phage. The total size of this chimera was designed to be packaged into P4 or P2 size heads with induction by P2 infection. The conversion of plasmid P4 ash8 (sid71) kmr to bacteriophage was proved by burst size determination experiment and CsCl buoyant equilibrium density gradient experiment. Integrase destructed P4 derivative, P4 ash8 sid71 kmr intS, was able to be constructed easily by in vitro DNA manipulation of P4 ash8 sid71 kmr. The plasmid stability experiment with P4 ash8 sid71 kmr if/tS showed that the integrase of P4 affects the stable maintenance of plasmid P4 state.

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Channel Equalization Algorithms for an WAVE System (WAVE 시스템을 위한 채널 등화 기법)

  • Kim, Yong-Sung;Seo, Chang-Woo;Hong, Dae-Ki
    • Proceedings of the KAIS Fall Conference
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    • 2008.11a
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    • pp.326-329
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    • 2008
  • 현재 IEEE 802.11p 규격은 기존의 IEEE 802.11a 규격을 기반으로 하여 표준화가 진행되고 있다. 기존의 IEEE 802.11a 규격은 무선랜 (WLAN: Wireless Local Area Network) 규격으로서 고속으로 이동하는 차량 통신환경 즉 WAVE (Wireless Access for Vehicular Environment) 환경에 그대로 작용할 경우 수신 성능이 급격히 떨어지게 되 는 문제점이 있다. 본 논문에서는 긴 훈련 심볼 (LTS: Long Training Sequence)을 이용하는 기존의 채널 추정 기법을 기반으로 하되 WAVE 채널처럼 빠르게 변화하는 채널에 대응하기 위해 일정한 심볼 주기 마다 미드엠블 (Mid-Amble)을 삽입하는 전송구조를 제안한다. 또한, 미드엠블 사이의 심볼들의 위상과 크기를 3차원 스플라인 보간법을 적용하여 추정하는 알고리듬을 제안한다. 제안된 알고리듬의 성능실험을 위해 WAVE 채널을 모델링하였으며 이러한 채널에서 성능실험을 수행하였다. 실험결과에 의하면 제안된 알고리듬은 빠른 시변 채널에서도 매우 우수한 성능을 나타냄을 알 수 있다.

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A Study on the High Viscosity Photosensitive Polyimide Degassing and Pumping System (반도체 생산공정을 위한 고점도 감광성 폴리이미드 탈포 및 공급시스템에 관한 연구)

  • Park, Hyoung-Keun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.2
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    • pp.1364-1369
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    • 2015
  • As the wire bonding process has been converted into BUMP process due to the high density integration of semiconductor chip, the telecommunication line connecting to semiconductor chip and external devices have become finer. As a result, a more precise work is necessary. However, it is difficult to control quantity given the nature of high viscosity of PSPI and the yield rate continues to decline due to the inflow of bubble. Therefore, this paper developed the D&P(degassing and pumping) system to remove and supply gas that is generated from coating the high viscosity photosensitive polyimide(PSPI) in the semiconductor BUMP process.