• Title/Summary/Keyword: 물 용매

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Antimicrobial Activity of the Aerial Part of Artemisia capillaris Extracts on the Food-Borne Pathogens (인진호 추출물의 식중독 세균들에 대한 생육억제 효과)

  • 이종기;서진종
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1227-1232
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    • 2003
  • The solvent extracts of the aerial part of Artemisia capillaris extracted by using several solvents with different polarities were prepared and their antimicrobial activities were examined. The antimicrobial activities and cell growth inhibitions were investigated to each strain with the different concentrations of the aerial part of Artemisia capillaris Acetone extract showed the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain were appeared to 20 mg/mL at Staphylococcus aureus and Bacillus subtilis, 40 mg/mL at Vibrio parahaemolyticus, and 80 mg/mL at Salmonella tyhimurium. The cell growth inhibitions were shown on Staphylococcus aureus, Bacillus subtilis, Vibrio parahaemolyticus, and Salmonella typhimurium for 48 hours. The acetone extract was further fractionated sequentially with hexane, chloroform, ethyl acetate, and butanol for purifying crude acetone extract. The solvent fraction of hexane, chloroform, ethyl acetate, and butanol showed the different antimicrobial activity, respectively.

Anticancer Effects of the Extracts of Oriental Melon (Cucumis melo L. var makuwa Makino) Seeds (참외(Cucumis melo L. var makuwa Makino) 종자 추출물의 항암 활성)

  • Kim, Jung-Hyun;Suh, Jun-Kyu;Kang, Young-Hwa
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.647-651
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    • 2012
  • The objective of this study was to investigate the anticancer effects of the extracts of oriental melon seeds. Various solvent extracts of oriental melon seeds were prepared and their anticancer effects were examined using in vitro MTT and CV assays. The anticancer effects of various extracts of oriental melon seeds were also examined in five human cancer cell lines including A549, AGS, HT-29, MCF-7 and HepG2. The ethanol extract of heated oriental melon seeds showed the potent cytotoxic effects especially against mouse hepatoma cell line(Hepa1c1c7), human hepatoma cell line(HepG2) and human breast cancer cell line(MCF-7). These data suggest that oriental melon seeds can be a promising anticancer agent against human liver and breast cancer.

Comparison of Physiological Activities of Radish Bud (Raphanus sativus L.) according to Extraction Solvent and Sprouting Period (추출용매 및 발아시기에 따른 무순 추출물의 생리활성 비교)

  • Han, Jin-Hee;Moon, Hye-Kyung;Chung, Shin-Kyo;Kang, Woo-Won
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.4
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    • pp.549-556
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    • 2015
  • This study extracted radish bud (Raphanus sativus L.) and investigated its nitrite scavenging activity, superoxide dismutases (SOD)-like activity, tyrosinase inhibition activity, xanthine oxidase inhibition activity, and angiotensin-converting-enzyme (ACE) inhibition activity according to extraction solvent and sprouting period. For nitrite scavenging activity, each extract recorded its highest level of 81.44~89.71% at pH 1.2. Radish bud extracts on sprouting days 4 and 8 showed greater scavenging activities than those on sprouting day 12 at pH 1.2 and pH 4.0. There were differences in scavenging activity according to extraction solvent based on water extract exhibiting improved scavenging activity. Ethanol extract recorded scavenging activity of 16.12% at pH 6.0, which was similar to those of ethanol and methanol radish bud extracts on sprouting day 12. SOD-like activity of radish bud extracts was in the range of 4.57~27.05%. For comparison purposes, SOD-like activity of L-ascorbic acid was 52.15%, which was higher than that of radish bud extracts. Acetone and methanol extracts showed high SOD-like activities on sprouting day 8. SOD-like activity of radish bud extracts on sprouting day 12 significantly decreased to 4.57~15.59%. Radish bud extracts recorded good tyrosinase inhibitory activities on sprouting 8 and 12, whereas methanol extracts recorded the greatest tyrosinase inhibitory activity at 62.65~84.89%. Radish bud extracts recorded xanthine oxidase inhibition activity of 21.26~29.52% on sprouting day 4, and acetone extracts showed the highest level of xanthine oxidase inhibition activity. Xanthine oxidase inhibitory activity tended to decrease with sprouting period compared early on. ACE inhibitory activity was in the range of 12.48~51.78% according to sprouting period and extraction solvent. Ethanol extracts on sprouting day 8 showed the highest ACE inhibitory activity of 51.78%. These results will hopefully contribute to research into the identification of materials and development of products for natural functional foods.

Photocatalytic Activity of ZnO Nanoparticles and Their Stability in Water Solvent (산화아연 입자의 광촉매 효과와 물 용매에서의 안정성)

  • Nam, Sang-Hun;Boo, Jin-Hyo
    • Journal of the Korean Vacuum Society
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    • v.22 no.3
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    • pp.138-143
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    • 2013
  • Recently, ZnO nanoparticles have been studied in various application fields due to their physico-chemical properties. In this study, we have researched on the ZnO photocatalytic activity by redox reaction. ZnO nanoparticles have low photocatalytic activity in comparison with $TiO_2$ nanoparticles because it has the disadvantage that the formation of $Zn(OH)_2$ in water solvent. Therefore, we were synthesized ZnO nanoparticles by spray-pyrolysis method, and then studied on stability in water solvent. At the results, the water treated-ZnO nanoparticles showed higher photocatalytic activity than non-treated ZnO nanoparticles because molecular $H_2O$ was increased onto the ZnO surface under the water treatment. Also, we confirmed that the ZnO nanoparticles synthesized by spray-pyrolysis method is very stable in the water solvent.

Dyability of Silk Fabrics with Juniperus chinenesis Extracts by Solvents (용매에 따른 향나무 부위 별 색소 추출물의 견직물에 대한 염색성)

  • Lee, Jung-Soon;Nam, Ki-Yeon
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2012.03a
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    • pp.69-69
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    • 2012
  • 지구상에는 약 40만 종의 식물이 존재하는 것으로 알려져 있으며 이 중에 는 질병의 치료를 위한 약재로 이용되거나 나무는 대부분 목재 등으로 이용된다. 향나무(Juniperus chinenesis L.)는 항균성 및 방충성이 뛰어난 상록수로서 목재는 조각재나 가구재로 사용되고 민간 및 한의학에서 다양한 증상의 약재로 쓰이고 있으며 부패한 냄새를 제거할 목적이나 향재 및 향료로도 쓰이고 있다. 열매의 정유성분은 화장품, 술, 캔디 등에 사용되며 cedrol은 향료보류제, 유분은 훈향료, 목부는 고혈압, 곽란, 심복통, 통기파혈에 쓰인다. 향나무는 폴레페놀 성분이 함유되어 있고 피톤치드가 많이 나오는 것으로 알려져 있으며 심재의 폴리페놀 화합물은 항암, 항균, 항알러지, 노화방지 및 심장질환 등을 예방하거나 지연시키는 등 광범위한 약리학적 활성을 나타내어 식품, 의약품, 화장품, 향료등 다양한 분야에 활용되고 있다. 향나무 추출물에 관한 연구로는 성분분석과 생리활성 등에 관한 의약학 분야의 다양한 연구와 항산화, 항균활성 등 약리작용에 연구가 최근 다양하게 이루어지고 있는 것을 볼 수 있다. 측백나무속에 속하는 나무들은 대부분 정유 성분을 함유하고 있는 특성으로 항균성 등 바이오 기능성 소재 개발을 위한 연구가 활발히 진행되고 있으나, 향나무 추출물의 염색성에 관한 연구는 찾아보기 어려운 실정이다. 이에 본 연구에서는 다양한 용매를 이용하여 향나무의 잎, 열매, 수피, 심재 각 부위 추출물을 이용하여 견직물에 대한 염색성을 살펴봄으로써 향나무 추출물을 활용한 천연염색과 염재로서의 가능성을 확인하고자 하였다. 실험 결과 증류수를 염액 용매로 염색한 결과 모든 추출물의 잎, 열매 염색포의 색상은 대부분 옅은 Y계열로 나타났으며 향나무의 수피와 심재 염색포는 대부분 적색기미가 강한 YR계열로 나타났다. 에탄올 혼합액을 염액 용매로 하여 염색한 결과 향나무의 잎과 열매의 색상은 대부분 Y계열의 색상을 나타냈고 유기용매 추출염색포에서 정유성분으로 추정되는 물질 때문에 균염이 어려웠으며 수피와 심재 염색포에서 대부분 R계열의 색상이 뚜렷이 나타났다.

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Volatile Compounds and Antiproliferative Effects of Dendropanax morbifera on HepG2 Cells (황칠나무의 휘발성 화합물 분석 및 HepG2 세포의 증식 억제 효과)

  • Yang, Seun-Ah;Garcia, Coralia V.;Lee, Ji-Won
    • Journal of Life Science
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    • v.27 no.5
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    • pp.561-566
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    • 2017
  • Dendropanax morbifera Lev. is known in Korea for its golden sap and medicinal properties. The many biological activities of the leaf and stem extracts suggest that this tree could be a valuable source of medicinal compounds for the treatment of various ailments such as dermatitis, migraines, dysmenorrhea, muscle pain, and infectious diseases. However, there is little information on the composition and biological activity of the volatile fraction of D. morbifera. Therefore, in this study, the volatile compounds in leaves, stems, and sap of D. morbifera were isolated using solvent and supercritical fluid extraction (SFE), and analyzed by gas chromatography/mass spectrometry to reveal their chemical composition and identify potential compounds of interest. Fifteen compounds were identified in the leaf extracts, whereas 29 and 3 compounds were identified in the stem and sap extracts, respectively. The volatile profiles obtained using solvent and SFE differed. Esters and aromatic hydrocarbons predominated in the solvent extract of leaves and SFE extract of stems, whereas the solvent extract of stems and SFE extract of leaves contained terpenoids. Limonene, ${\alpha}$-pinene, and ${\beta}$-myrcene were identified in the volatile extract of sap, with limonene representing 96.30% of the total peak area. In addition, the antiproliferative effects of the solvent extracts of leaves and stems were evaluated, revealing that these solvent extracts were particularly effective in decreasing the proliferation of HepG2 cells.

Effect of Organic Solvents on Lipase for Interesterification of Fats and Oils (유지의 에스테르교환에 있어서 유기용매가 리파제에 미치는 영향)

  • Kwon, Dae-Y.;Rhee, Joon-S.
    • Korean Journal of Food Science and Technology
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    • v.17 no.6
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    • pp.490-494
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    • 1985
  • The effect of organic solvents on the stability and catalytic activity of the microbial lipase from Rhizopus arrhizus for interesterification of fats and oils has been examined. The reaction system used was nonaqueous solvent system (two phase system). The solvents examined were 5 hydrocarbons (n-hexane, n-heptane, n-octane, isooctane, and cyclohexane) and 3 ethers (diethylether, diisopropylether, di-n-butylether). The results revealed that diisopropylether and isooctane are superior to the other solvents examined for interesterification of fats and oils in two phase systems.

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Removal of Organic Pollutants from Aqueous Solution by Hollow Fiber Module (중공사모듈에 의한 수용액으로부터 유기오염물의 제거)

  • 유홍진
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.4 no.2
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    • pp.114-119
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    • 2003
  • This study is to remove several organic pollutants from wastewater by non-dispersive membrane solvent extraction technique. The distribution coefficients of several solvents were determined and the experimental system was operated counter-currently and cocurrently with respect to the aqueous phase and solvent. In these experiments, as the flow rate of aqueous solution inclosed, due to being shortened contact time to solvent, the rate of removal of organic pollutants decreased and as the flow rate of solvent increased, the rate of removal increased. Meanwhile, the rate of removal of organic pollutants for the countercurrent flow system was higher than that for the occurrent flow system.

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Biological Activities of Various Solvent Extracts of Seomaeyakssuk (Artemisia argyi H.) (섬애약쑥 용매별 추출물의 생리활성)

  • Kim, Dong-Gyu;Kang, Jae Ran;Shin, Jung-Hye;Kang, Min-Jung
    • Journal of Life Science
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    • v.29 no.11
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    • pp.1241-1250
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    • 2019
  • Artemisia argyi H. has been used for centuries as a traditional medicine and food supplement in Asian countries. The objective of this study was to investigate the physiological activities of Artemisia argyi H. extracts prepared by butanol, chloroform, ethyl acetate, ethyl ether, hexane, and methanol extraction. We evaluated total phenol and flavonoid content, antioxidant activity, nitric oxide (NO) and reactive oxygen species (ROS) release, and osteoclastogenesis inhibition. The total phenolic and flavonoid contents were highest in the methanol extract (49.46 mg GAE/g and 24.32 mg QE/g, respectively). The methanol extracts also had the highest antioxidant activity (DPPH and ABTS radical scavenging ability and ferric reducing antioxidant power), while the hexane extract had the lowest. The release of NO and ROS was dose-dependently decreased by pre-treatment with all solvent extracts. At the same concentrations, the ethyl acetate and butanol extracts showed higher inhibition of NO and ROS production when compared with the other extracts. The butanol extract, at a concentration of $20{\mu}g/ml$, inhibited about 89% of the activity of the osteoclast marker, tartrate resistant acid phosphatase (TRAP). These results suggest that butanol extracts of Artemisia argyi H. may be effective natural medications for the prevention and treatment of osteoporosis.

Antioxidation activity of residue after omija (Schisandra chinensis) juice extract (오미자(Schisandra chinensis) 착즙박 추출물의 항산화 활성)

  • Park, Bo Na;Lee, Jin-Won
    • Journal of Applied Biological Chemistry
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    • v.60 no.2
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    • pp.95-100
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    • 2017
  • The physicochemical properties and schizandrin contents of various solvent ($H_2O$, 50% EtOH, 75% EtOH, 95% EtOH) extracts from residue after Omija juice was investigated using total polyphenol contents (TOC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (RAS), anthocyanin contents (ANC), and schizandrin contents level (SCL). Total polyphenol contents, radical scavenging activity, and anthocyanin contents of 50% EtOH extract were the highest among all residue after Omija juice extracts, and was 16.70 mg/mL in the TOC and 86.16% in the DPPH-RAS. This meant that 50% EtOH extract from residue after Omija juice had more available antioxidant matters. As extraction time increases all extract treatments significantly reduced in the ANC contents (p <0.05). Amount of the SCL were observed higher value in 95% EtOH extract of residue after Omija juice.