• Korean Journal of Veterinary Service
• /
• 제40권2호
• /
• pp.107-117
• /
• 2017

This study was conducted to investigate of hepatoprotective effect of dandelion water extract (DWE) according to repeated administration of thioacetamide (TAA) induced hepatotoxicity in Spraque-Dawley rats. Thirty rats were randomly assigned to 5 groups; normal control, DWE-control, TAA-control (TAA injection during the feeding of normal diet), TAA&DWE600 (TAA repeated injection during the feeding of DWE 600 mg/kg BW), TAA&DWE1200 (TAA repeated injection during the feeding of DWE 1,200 mg/kg BW). Rats in DWE-control and TAA&DWE groups were treated with DWE (600 or 1,200 mg/kg BW daily) by gavage for 20 days (twice a day). All the rats in the TAA-control and TAA&DWE groups were repeated injection of TAA (100 mg/kg BW) into the abdominal cavity 3 days interval and 12 hrs later, all rats were sacrificed. At the same time, normal control and DWE-control groups were injected normal saline. In TAA&DWE groups, serum alanine and aspartate aminotransferase (ALT, AST) were significantly decreased and triglyceride (TG) synthesis was significantly increased compared to TAA group. As well as total billilubin and GGT were slightly decreased by the treatment of DWE. Lipid peroxidation (MDA) concentration was significantly decreased and hepatic GSH content was slightly or significantly increased in the TAA&DWE groups compared to TAA group. Hepatic anti-oxidative enzyme activities, such as GSH, GST, SOD and catalase were slightly or significantly elevated by the treatment of DWE. According to these results, When dandelion extract was long term supplied, it could be used as a potential protective material for a longer time liver damage by repeated adminstration of the TAA.

• Korean Journal of Veterinary Research
• /
• 제39권6호
• /
• pp.1081-1090
• /
• 1999

To establish the protocol of a standardized exercise test for evaluating exercise intolerance and degree of fitness in Thoroughbred racehorses, we examined serum lactate concentrations related to exercise intensities using the high speed treadmill. Twelve clinically healthy Thoroughbred racehorses with or without previous training or racing history were assigned to two gorups, fit and unfit group, respectively. The protocol used for the standardized exercise test was consisted of two stages : stage of warm-up and that of acceleration. During the warm-up, the horses exercised 5 min at 1.8m/s and 3 min 3.4m/s without inclination. At the acceleration stage, exercise test was performed at 10% slope and the speed was increased from the initial 5m/s to the maximal speed which each tested horse could keep up with. The speed was increased with incremental steps of 1 m/s every minute. During the last 15 sec of each step, blood samples were collected for serum lactate determination. $V_{max}$(maximal treadmill speed which tested horses could keep up with) of the fit group ($10.93{\pm}0.33m/s$, mean${\pm}$SE, n = 6) was higher than that of the unfit group ($9.52{\pm}0.23m/s$, mean${\pm}$SE, n = 6). Serum lactate concentrations increased exponentially according to exercise intensities. $V_{La4}$(speed producing a serum lactate concentration of 4mmol/l) of the fit group, $6.45{\pm}0.26m/s$, was higher than that of the unfit group, $5.45{\pm}0.23m/s$. $La_{peak}$(peak plasma lactate concentration during the exercise test) was lower in the fit group ($20.34{\pm}1.62mmol/l$ at 1 min after maximal intensity exercise) than in the unfit group ($24.78{\pm}1.09mmol/l$ at 2 min after maximal exercise step). $t_{50%}$(time required for the recovery of lactate concentration to be one-half of $La_{peak}$ after maximal exercise) of the unfit group and the fit group were 40.0 and 18.0 min, respectively. Therefore, the protocol of the incremental standardized exercise test utilized in this study seems to be reliable for the assessment of fitness and exercise intolerance for the Thoroughbred racehorses.

• Journal of Korean Society on Water Environment
• /
• 제24권4호
• /
• pp.488-498
• /
• 2008

Soil and Water Assessment Tool (SWAT) model have been widely used in simulating hydrology and water quality analysis at watershed scale. The SWAT model extracts topographic feature using the Digital Elevation Model (DEM) for hydrology and pollutant generation and transportation within watershed. Use of various DEM cell size in the SWAT leads to different results in extracting topographic feature for each subwatershed. So, it is recommended that model users use very detailed spatial resolution DEM for accurate hydrology analysis and water quality simulation. However, use of high resolution DEM is sometimes difficult to obtain and not efficient because of computer processing capacity and model execution time. Thus, the SWAT Topographic Feature Extraction Error (STOPFEE) Fix module, which can extract topographic feature of high resolution DEM from low resolution and updates SWAT topographic feature automatically, was developed and evaluated in this study. The analysis of average slope vs. DEM cell size revealed that average slope of watershed increases with decrease in DEM cell size, finer resolution of DEM. This falsification of topographic feature with low resolution DEM affects soil erosion and sediment behaviors in the watershed. The annual average sediment for Soyanggang-dam watershed with DEM cell size of 20 m was compared with DEM cell size of 100 m. There was 83.8% difference in simulated sediment without STOPFEE module and 4.4% difference with STOPFEE module applied although the same model input data were used in SWAT run. For Imha-dam watershed, there was 43.4% differences without STOPFEE module and 0.3% difference with STOPFEE module. Thus, the STOPFEE topographic database for Soyanggang-dam watershed was applied for Chungju-dam watershed because its topographic features are similar to Soyanggang-dam watershed. Without the STOPFEE module, there was 98.7% difference in simulated sediment for Chungju-dam watershed for DEM cell size of both 20 m and 100 m. However there was 20.7% difference in simulated sediment with STOPFEE topographic database for Soyanggang-dam watershed. The application results of STOPFEE for three watersheds showed that the STOPFEE module developed in this study is an effective tool to extract topographic feature of high resolution DEM from low resolution DEM. With the STOPFEE module, low-capacity computer can be also used for accurate hydrology and sediment modeling for bigger size watershed with the SWAT. It is deemed that the STOPFEE module database needs to be extended for various watersheds in Korea for wide application and accurate SWAT runs with lower resolution DEM.

• Journal of Life Science
• /
• 제21권4호
• /
• pp.542-548
• /
• 2011

Food-dependent exercise-induced anaphylaxis (FDEIA) is a distinct form of food allergy induced by physical exercise. It is typified by the onset of anaphylaxis during exercise, which is preceded by the ingestion of causal food allergens. Diagnosis of FDEIA is heavily dependent on clinical history. To describe the physiopathological mechanism, etiologic factors, and clinical manifestations, we evaluated the spleen index, proliferation assay of lymphocyte, ROS, ASAS, and cytokines levels in sensitized and exercise-trained mice. One-hundred mice were bred in the animal lab at D and P university under controlled conditions [$22{\pm}2^{\circ}C$, RH 45-55%, and a 12-hour photoperiod]. Animals are 7-weeks-old at the time of study and were fed a standard commercial chow diet from 09:00 to 15:00 over the 8-week study period. The mice were allowed access to distilled deionized water ad libitum. Daily food intake and weekly body gains were routinely recorded throughout the experimental period using computing scale (CAS). Mice were divided into the control group (S; control sensitized, n=25), 30 min swim training group (S30, N=25), 50 min swim training group (S50, N=25), and 80 min swim training group (S80, N=25). The results were as follows: Spleen index showed the highest level in the S80 group compared to other groups; this level was exercise-dependent. In proliferation assay of Med and OVA, the S80 group showed the highest level compared to the other groups; this level also was exercise intensity- dependent. Peritoneal ROS and IL-4 showed a statistically significant difference compared to S; however, there was no significant differences in ROS among S30, 50, and 80. From the results, we concluded that FDEIA is correlated with exercise intensity based on the levels of peritoneal ROS and cytokine profiles.

• Journal of Life Science
• /
• 제19권8호
• /
• pp.1055-1061
• /
• 2009

${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors are widespread throughout the central nervous system and appear to serve as synaptic receptors for fast excitatory synaptic transmission mediated by glutamate. Their modulation is believed to affect learning and memory. To identify the interaction proteins for the AMPA receptor subunit glutamate receptor-interacting protein 1 (GRIPl), GRIP1 interactions with armadillo family protein p0071/plakophilin-4 were investigated. GRIP1 protein bound to the tail region of p0071/plakophilin-4 but not to other armadillo family protein members in a yeast two-hybrid assay. The "S-X-V" motif at the carboxyl (C)-terminal end of p0071/plakophilin-4 is essential for interaction with GRIP1. p0071/plakophilin-4 interacted with the Postsynaptic density-95/Discs large/Zona occludens-1 (PDZ) domains of GRIPI in the yeast two-hybrid assay, as is indicated also by Glutathione S-transferase (GST) pull-down, and co-immunoprecipitated with GRIP1 antibody in brain fraction. The findings of this study provide evidence that p0071/plakophilin-4 is an interactor of GRIP1.

• Journal of Life Science
• /
• 제24권8호
• /
• pp.910-914
• /
• 2014

For the identification of the Jeju black cattle, Hanwoo and imported beef, we performed a multiplex polymerase chain reaction (PCR) associated with microsatellite (MS) and melanocortin 1 receptor (MC1R) gene. The MC1R gene plays an important role in regulation of the melanin synthesis within mammalian melanocytes. MC1R encoded by extension (E) locus was almost fixed with recessive red e allele in the Hanwoo. We estimated that the specific genotypes ($E^+/E^+$, $E^+/e$) of MC1R gene were characteristic genotypes of Jeju black cattle. But the PCR products resulted from using the MC1R gene derived primers only are not sufficient to identify Jeju black cattle from other relatives. We performed two times of successive multiplex PCR to provide a more accurate result for the identification of Jeju black cattle. The results suggest that two types of successive multiplex PCR methods using MC1R gene and Microsatellite derived primer set will be more useful to identification of Jeju black cattle, Hanwoo and imported beef.

• Journal of Life Science
• /
• 제23권7호
• /
• pp.941-946
• /
• 2013

Local protein synthesis at subsynaptic sites plays a key role in the regulation of the protein composition in local domains. In this study, we carried out immunocytochemistry of cultured rat hippocampal neurons in various developmental stages to investigate the expression of eIF4E and its binding protein, eIF4EBP1. Both proteins were distributed in dendrites. In addition, eIF4EBP1 was highly expressed in the nucleus throughout the development, whereas eIF4E was not expressed in the nucleus. Punctate expression of eIF4E and eIF4EBP1 was evident in DIV 3. The colocalization rates of eIF4E or eIF4EBP1 puncta with PSD95 were higher in the dendrogenic than in the mature stages. In contrast, the colocalization rates of eIF4E and eIF4EBP1 puncta were higher in the mature than in the dendrogenic stages. As eIF4E is inactive when it is bound to eIF4EBP1, these data indicate that most dendritic eIF4E's are active during development but that they are mostly under inhibition in mature neurons.

• Journal of Life Science
• /
• 제23권4호
• /
• pp.586-594
• /
• 2013

Post-translational O-GlcNAc modification (O-GlcNAcylation) of serine or threonine is a new protein modulation mechanism. In contrast to the classical glycosylation, O-GlcNAcylation occurs in a one-step transfer of O-GlcNAc on both nuclear and cytoplasmic proteins. In contrast to the general consensus that O-GlcNAc is a final modification, a recent paper (J Proteome Res. 2011 10:2725-2733) showed the presence of O-GlcNAc-P on a synaptic assembly protein AP180. This finding raises a fundamental question about its prevalence. To address this question, we used proteomics to identify those proteins that were phospho-signal enriched by GlcNAc kinase (NAGK). Comparison of pDsRed2-$NAGK_{WT}$-transfected HEK293T cell extract with pDsRed2-$NAGK_{D107A}$-transfected control culture revealed 15 phospho-signal increased spots. Excluding those spots that had no detectable amount of protein expression yielded 7 spots, which were selected for ID determination. Among these, two duplicate spots (two $HSP90{\beta}$ and two ENO1 spots) were shown to be O-GlcNAcylated, two (dUTP nucleotidohydrolase mitochondrial isoform 2, glutathione S-transferase P) were not known to be involved in O-GlcNAcylation, and one (heat shock protein gp96 precursor or grp94) was a glycoprotein. The increase in the phospho-levels of O-GlcNAc by NAGK strongly indicates that these proteins are phosphorylated on O-GlcNAc. Our present data support the idea that O-GlcNAc is not a terminal modification.

• Communications of Mathematical Education
• /
• 제13권1호
• /
• pp.73-96
• /
• 2002

수학적인 사고력과 창의력이 강조되고 있는 요즈음 수학교육에서는, 이산수학적인 영역이 담당해야 할부분이 더욱 많아진 것으로 생각된다. 이에 발맞춰, 최근에 이산수학에 관한 연구가 활발해지고 있다. 그러나, 아직 초등학교에서 적절히 사용할 수 있는 별도의 이산수학 관련 서적이나 연구 문헌이 없어 아동들의 이산수학에 대한 관심과, 수학 성적과 이산수학의 문제 해결력과의 관계에 대하여 조사해 보았다. 이산수학의 문제들을 구성하여 아동들에게 예고 없이 평가하고 문제에 대한 수학적인 태도를 질문을 통하여 알아보고, 수학 실력이 우수한 학생과 그렇지 못한 학생들과의 이산수학 문제 해결력의 관계를 알아보고자 다음과 같은 연구 내용을 설정하였다. 이를 살펴보면 첫째, 초등 수학교육에서 이산수학에 대한 학생들의 반응에 대하여 생각해 본다. 둘째, 수학 성적과 이산수학 문제 해결과의 관계를 생각해 본다. 이상의 연구 문제를 해결하기 위해, 문헌 연구를 통하여 이산수학에 관련된 초등학교 내용을 소개하고, 문항을 구성하였다. 소개된 주제 중에서 4개의 주제(수 세기, 한 붓 그리기, 지도 색칠하기, 최소 거리 ${\cdot}$ 비용 수형도)를 선정하여 10개의 문항을 작성하였다. 조사 연구를 위한 대상은 서운 시내 2개 초등학교 5, 6학년 2개 반을 선정하였다. 각 문항의 정답율은 백분율(%)에 의하여 분석하였는데 그 결과를 살펴보면, 첫째, 수 세기의 정답율은 첫 번째 문항의 정답율이 낮았을 뿐, 다른 문항들의 정답율은 비교적 좋게 나타난 것으로 보아 문제를 이해하기 쉽게 구성하는 것이 중요하다는 것을 알게 되었다. 둘째, 한 붓 그리기와 지도 색칠하기의 문제들의 정답율은 상당히 높게 나타났는데, 그러한 것은 아동들이 직접 다양한 방법으로 시도해 봄으로써 문제를 해결할 수 있었기 때문인 것 같다. 또한 이러한 유형의 문제들은 아래 학년에도 투입해 볼 수 있을 것 같다. 셋째, 최소거리 ${\cdot}$ 비용 수형도의 문제에서는 난이도가 높은 이유도 있지만 문제 이해를 완전히 하지 못해 정답율이 무척 낮게 나온 것으로 생각된다. 넷째, 수학 성적이 높은 학생들이 대체적으로 문제 해결력이 높았던 것으로 나타났으나, 몇몇 학생들은 정반대의 결과가 나와 특이한 시사점을 제공했다. 그러한 이유로는 정형화된 문제들을 선호하고 쉽게 해결하는 아동들과, 그렇지 않은 아동들 사이의 문제 접근 방법의 차이라고 생각된다. 본 연구를 통하여 다음과 같은 제언을 하고자 한다. 첫째, 이산수학에 관련된 많은 문항을 개발하여 아동들에게 확대 투입함으로써 수학 수업의 효과와 문제 해결력을 높일 수 있을 것이라 생각된다. 둘째, 수학 실력이 떨어지는 아동들에게 보다 흥미있는 이산수학적 문제들을 제시함으로써 수학에 대한 자신감과 흥미를 높일 수 있을 것이라 생각된다. 셋째, 초등학교 과정에 알맞은 이산수학의 다른 주제도 학습 지도안과 그와 관련된 문제들을 개발하는 연구가 진행되어야 하겠다.

• Journal of Korean Society of societal Security
• /
• 제4권2호
• /
• pp.57-64
• /
• 2011

Although it is uncertain that the cause of changed pattern of the natural disaster related to water (i.e. flood and drought) is due to excessive carbon dioxide yielded from economic activity or the increased number of sunspots, it is apparent that there have been unusual climate change that directly affects the water resource management. Due to such a frequent unusual weather activities, there have been increased natural disaster and the most direct and major reason is considered as climate change. As we see, the climate change necessarily causes social costs. Especially, the effects on the water resource due to flood and drought take the considerable part of such costs. Therefore, this study is basic work to develop a new economic analysis technique to be used in pursuing appropriate adaptation project in field of the amount of cost damage through analysis of the effects of the climate change on the water resource. The models appeared in many reports for cost assessment of climate change were various (e.g., PAGE, DICE, AIM, IMAGE, MERGE, and etc.) and this report summarizes general characteristics of each model. To assess the effects of climate change of the water management, we defined the field of the water management on climate change. The results help post-study in field of the climate change's social-economic effect assessment, can be employed for the prioritizing process of the national fund's investment.