• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.461-467
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• 2007

The research on food habits of the Fire-bellied toad, Bombina orientalis, distributed in Korea was done targeting a total of 487 individuals in 4 inland areas and 8 areas of Jeju island from April 1990 to September 1992. The survey results showed that there was no difference In the number of the preys of the Korean Fire-bellied per individual between the inland areas and Jeju island and the toad preyed on 1 to 3 individuals on an average, but there happened a case where some Fire-bellied toads in Jeju preyed on as much as 94 individuals. There were 13 orders surveyed in the kinds of prey of the Fire-bellied toad inland, and 10 in Jeju island with a high predation frequency and the number of the preyed individuals of Coleoptera and Hymenoptera. The predation frequency of the two populations was similar in terms of prey taxa, but there appealed a different aspect in the number of the individuals by prey taxa. In short, the inland population of Fire-bellied toad was found to eat more various sorts of preys, not satisfied with limited sorts of preys in comparison with the Jeju toad. There appeared a difference in eating habits by month between the two populations; the feeding duration of the inland population was from May to October, but the Jeju population was from April to September. Among the kinds of prey taxa, Coleoptera and Hymenoptera were equally distributed to the two populations of Korean Fire-bellied toad for this research purpose during the survey period. The number of orders, predation frequency and the number of the prey taxa by one population prey were rather higher in the summer. Therefore, it is concluded that the most active period of predation of the Korean Fire-bellied toad is summer.

• Korean Journal of Environmental Biology
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• v.26 no.1
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• pp.22-29
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• 2008

Bombina orientalis, or Korean fire-bellied toad is belonging to Class Amphinia, Order Anura, Family Bombinatoridae, Genus Bombina. Ecological characteristics, such as small size, slow moving and swimming, gentle attitude to other species, under laboratory and/or wild condition lead the toad as suitable experimental animals with easy handling. Seasonal reproductive biology except breeding season (usually from Apr. to late Jun.) are unknown still caused by sampling methods for hibernating specimens have not been established yet. Most species of amphibian, include B. orientalis, are declined around the world related with global warming, therefore, wild caught specimen for any purposes must be limited controled soon. Even though, some pre-studies of biotope for increasing biodiversity have been reported, construction of an artificial biotope to get natural breeding cycling specimen for study of amphibian reproduction is unique modelling and only trial. In an effort to set year-round sampling for study of reproduction of B. orientalis, we suggest protocols for planning, construction manual and SOP(Standard Operation Process) to manage amphibian biotope. During pilot operation of a designed and constructed biotope, the seasonal acclimation during winter hibernation of B. orientalis were successful. It will be helpful for studying the reproductive biology of wild amphibians in temperate region and allow to get reasonable research conditions in amphibian laboratory.

• The Korean Journal of Zoology
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• v.16 no.3
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• pp.193-201
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• 1973

A cellulose acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase isozymes. The LDH and MDH isozymes in the tissues of the brain, heart, stomach, skeletal muscle and liver of the six species of Anura examined show the species specific patterns which differ from those of mammals and birds. Two isozymic forms of LDH and MDH exist in both Rana nigromaculata and Rana nigromaculata coreana, respectively, with almost the same pattern. LDH of Bombina orientalis has five isozymic forms, and that of Rana temporaria ornativentris contains four isozymes. Bufo sp. has 3 to 5, and Rana rugosa has 3 to 4 isozymic bands according to the tissues. MDH's of all animals have two isozymic forms with different spacing on the zymograms.

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.134-134
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• 2005

• 한국전자현미경학회:학술대회논문집
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• 1999.05a
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• pp.70-70
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• 1999

• The Korean Journal of Physiology
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• v.23 no.1
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• pp.79-87
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• 1989

Molecular property as well as biological activities of bombesin-like substance (BBS-LS) isolated from the skin of B. orientalis in Korea was compared with those of synthetic BBS-14. BBS-LS in the crude extract was applied on a Sephadex G-50, superfine column $(1.6{\times}112\;cm)$. On chromatogram, the first peak (3.5% of BBS-LS) was found ahead of synthetic GRP-27, and the second peak (rest) between synthetic GRP-27 and synthetic BBS-14. The main form of BBS-LS was successfully purified by using a column of alkaline alumina followed by sequential gel-filtrations on a column of Sephadex G-10, fine and a column of Sephadex G-50, superfine. Chromatographic analysis of the purified BBS-LS using a column of Sephadex G-50, superfine and reversed phase HPLC revealed that the main form of BBS-LS in the skin of B. orientalis could be distinctly different from either BBS-14 or GRP-27 in molecular size. The purified BBS-LS exerted biological activities quite identical to those of synthetic BBS-14. The results of the present investigation indicate that the skin of B. orientalis contains BBS-LS composed of two distinct forms. The main form of BBS-LS purified in the present study is heterogenous to both synthetic BBS-14 and GRP-27 in molecular size but identical to BBS in biological activities.

• Proceedings of the Zoological Society Korea Conference
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• 1998.04a
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• pp.43.1-43
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• 1998

• The Zoological Society Korea : Newsletter
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• v.12 no.2
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• pp.84.2-84
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• 1995

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.05a
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• pp.38-38
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• 2004

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2005.12a
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• pp.36-37
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• 2005