• Journal of Chest Surgery
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• v.31 no.3
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• pp.266-270
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• 1998

The management of malignant pleural effusion is a difficult problem. Tetracycline was probably the most commonly used sclerosing agent, but parenteral tetracycline is no longer available. This prospective study was designed to determine the efficacy of insufflated talc in producing plerodesisin patients with malignant pleural effusions. Fifteen patients managed between March 1995 and March 1997 underwent thoracoscopy and had 4 gm sterile asbestos-free talc insufflation throughout the pleural space. The mean interval between the procedure and last follow-up was 101 days(range: 25-233 days). The results of treatment were clinically and radiologically compared with pre-operative status. The results were as follows: 1. The patients were 6 men and 9 women with a mean age of 55.1 years(range: 31-74 years). The primary tumors were lung cancer in 10 patients, breast cancer in two, hepatoma in one, stomach cancer in one, and adenocarcinoma of unkown origin in one. 2. Cancer cell positive ratio was 36% in sputum cytology study and 100% in thoracoscopic biopsy. 3. Symptoms improved in 94% of patients treated by talc pleurodesis. 4. After 30 days of the procedure, there was radiologically no recurrence of plural fluid in 94% patients. 5. There were wound infection in one, respiratory failure in one. In conclusion, the insufflation of 4 gm sterile asbestos-free talc in the pleural space is an effective method to control malignant pleural effusions and patients selection is important factor for reexpansion the lung.

• Journal of Conservation Science
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• v.23
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• pp.81-93
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• 2008

The analysis of ancient DNA (aDNA) in paleogenetics has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. In this study, paleogenetic analyses were carried out on the human skeletal remains from a historical cemetery site in Myeongam-ri, Asan, Korea. Archaeological records show that this particular location had been used as a habitation or mortuary site as early as the Bronze Age and up until the Joseon Dynasty. Human remains of twenty individuals out of forty-nine tombs from the Goryeo to Joseon Dynasty were selected for the analysis of this study. In order to identify the genealogy of the population and traditional burial pattern of the cemetery, we conducted comparative analyses of the hyper variable regions (HVRs) in mitochondrial DNA (mtDNA) of each sample. A number of cautious steps were taken at all experimental stages in order to avoid erroneous recombination by the segmental and modern contaminations derived from the researchers. We sequenced segmental amplicons of HVRs andassigned relevant haplogroups according to the sequence polymorphism on the basis of the known mtDNA database. The result shows that diverse haplogroups were unexpectedly present in the small population group of the Myeongam-ri site. This diversity appears to be related to the geographical conditions and archaeological properties of the Myeongam-ri site.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.297-305
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• 2008

A series of experiments were conducted to suggest efficient in vitro germination conditions to improve germination rate and aseptic seedling production in chicory and garland chrysanthemum. For this purpose, various levels of NaOCl concentration and soaking treatment time combinations were tasted. Fifteen minutes of soaking treatment with 5% NaOCl solution significantly improved germination and seedling formation percentages and prevented contamination in both chicory 'Precole' and 'Chiavari' and garland chrysanthemum 'Okiku 3'. There was no significant difference in germination response between MS and 1/2 MS medium compositions, but germination and seedling formation were delayed as sucrose concentration increased. Although petri-dish among various culture containers gave rise to improved germination responses in chicory 'Precole', there was no significant difference in germination responses among culture container types in chicory 'Chiavari' and garland chrysanthemum 'Okiku 3'. The ultrasonic treatment stimulated germination and seedling formation in both chicory cultivars but there was no effect in garland chrysanthemum 'Okiku 3'. The results suggested 15 minutes with 5% NaOCl for seed sterilization, 1/2 MS medium with no sucrose and petri-dish as proper medium composition and culture container, and 120 minutes with 5% NaOCl solution of ultrasonic treatment as promoting method for in vitro germination in chicory and garland chrysanthemum.

• Journal of Korean Orthopaedic Sports Medicine
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• v.4 no.1
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• pp.55-59
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• 2005

Purpose: To evaluate the drainage effect of silk suture material following aspiration of the bursa as an early treatment of chronic prepatellar bursitis. Materials and Methods: Twelve cases, which have over two weeks of history and over one year of follow-up, were investigated. The average duration of follow-up was 18.3 months. The average symptom duration before introduction into this study was 2.2 months. With an aseptic technique, the aspiration of the bursa was done with spinal needle or injection needle and syringe and then the insertion of silk suture material through the aspiration needle was performed. Over one year follow-up, recurrence, infection, pain, and limitation of range of motion were investigated by telephone interview. Results: Redness around the insertion site of silk suture material was found in all cases, but there was no development of active infection in eleven cases. At five days after procedure, a supprative infection was developed in one case. The results were considered satisfactory in 92% of cases and the average duration of treatment is 14.5 days. Conclusion: The drainage with silk suture material following aspiration of the bursa is effective and less invasive method in the early treatment of chronic prepatellar bursitis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.56-60
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• 1976

In order to find effective seed collection method from cultivated Porphyra, benthic diatom elimination, culturing conditions of Conchocelis, liberation of conchospores and treatment of the fronds to obtain monospores have been studied. Elimination of benthic diatoms from Porphyra fronds is successfully performed by careful brushing the fronds in sea water and freshwater alternatively. For the culturing of Conchocelis Schleiber's solution enriched with only soil extracts, vitamins and Fe-EDTA was satisfactory. Growth under 16 hours illumination is 1.29 times faster than those under 10 hours illumination. When the culturing water was airated the growth was $1.41\~l.50$ times faster than the growth in stagnant water. Total amount of conchospores liberated from Conchocelis which has been cultured under airation was much more than those of conchospores under stagnant condition. Effective liberation of monospores was observed in the fronds which have been dried in air for 6 hours $(21.23\~24.19\%\;water\;content)$.

• Korean journal of applied entomology
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• v.13 no.2 s.19
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• pp.71-75
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• 1974

A study was conducted to investigate the relationships between the rice weevil and associated storage molds. The results are as follows; 1. All of the developmental stages of the rice weevils are carrying some storage molds in their bodies, and the order of magnitute in the number was the adult, larva and pupa. 2. The molds persist in the body of the rice weevil for 10 days who.1 they were fed on the mold free wheat, and the most persistant mold species was A. candidus. 3. When the mold free weevils were reared on the pure culture of the molds on the wheat, the number of eggs laid by the Lveevil were the greatest for A. candidus. following A. ruber, and the . least number Lvere obtained with A. niger 4. The rice weevil could complete in the pure mold culture on the wheat except for A. niger where the larvae had developed by 2nd or 3rd instars. 3. The shortest developmental periods was obtained with A. candidus and the first adult emerged in 4th week. 6. The unfavorable effects of A. niger on the development of the rice weevil might be associated with the fast growth of the mold together with some unknown effects. 7. There seems to be a protocooperative interaction between these two oraganisms having been developed through the long evolutionary course in common habitat.

• Journal of Korean Academy of Nursing
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• v.2 no.1
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• pp.141-157
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• 1971

The peripheral infection is one of the major causes of maternal death, and although it is preventable through an effective prenatal as well as postnatal care its morbidity is increasing due to bacterial resistant to an effective antibiotics. The primary purpose of this study was to investigate the peripheral morbidity of 949 parturients who were admitted to the Obstetrics and Gynecologic department of Ewha Woman's University Hospital from January 1971 to September 1971. Among the 949 parturients, especially 40 normal parturients were selected (20, control soup was given complete aseptic care during labor and delivery and post delivery: 20, compare group was given the ordinary care practiced during labor and delivery and maternal ward of Ewha Woman's University Hospital) for bacteriologic test on vaginal flora twice, on admission and on complete cervical dilatation of each parturient. The results obtained from this study were as follows; 1. Majority of parturients age were 21 to 35 years old(90.83%), and educational level of 949 parturients was above high school. A large number of parturients socioeconomic level (according to their husbands' job) were moderate. 2, Among the 949 parturients, multipara (55.9%) were a little more than primipara (44. 1%) and 38.84% of parturients had experienced aborition. 3. In deliverty types, normal deliveries (804 cases) were more than cesarean section deliveries (145 cases) The peripheral morbidity after normal deliveries was 0.5%, and cesarean section deliveries, 23.45%. 4. Among 949 parturients incidence of hemorrhage eases (500cc or more bleeding) showed the higher peripheral morbidity (24.86%) than other cases (bleeding less than 500cc, 7.83%). 5. The majority of parturients (81.03%) had teen taking antenatal care, but most of them were taken irregular antenatal care. On the other hand, on admission, the parturients with complication were 30.32%, and their peripheral morbidity showed much higher (7.02%) than those with no complication (2.71%). 6. The incidence of peripheral morbidity in premature ruptured membrane was higher (10.91%) than normal parturienta (1.73%). 7. In the result of aseptic care during labor and delivery and post delivery, the number of cultured bacteria was legs in control group than Compare group (in control group, on admission 17, on complete cervical dilatation 12: in compare group, on admission 21 on complete cervical dilatation 21) . The most common bacteria were Staphylococcus (control group 14 on admission, compare group 16 on admission), and next Streptococcus, E- Coil, Bacillus Subtilis, in order. Also in control group the number. of colony were reduced (43%) more than in Compare group. Transient temperature elevation from 37℃ to 37.4℃ were noted in compare group (50%) than in control group (30%), and there was no one indicated above 38℃. In conclusion, the aseptic care is the test way of preventing peripheral infection as well as decreasing the puerperal morbidity. Therefore the most important nursing care is the aseptic care for each parturient during labor and delivery and peat delivery and also all the instruments must be cleaned and sterilized.

• Journal of the Korean Recycled Construction Resources Institute
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• v.5 no.4
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• pp.496-502
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• 2017

The purpose of this study is to investigate the applicability of surface-wave techniques for the evaluation of the crack-repairing performance of an epoxy injection method in concrete. In this study, box-shaped concrete specimens with four different crack depths were made with identical mix proportions. The specimens with different crack depths were completely repaired using the same epoxy injection method. The spectral energy transmission ratio of surface waves is used as an index to differentiate the effects of crack depth and crack-repairing performance. The decrease of spectral energy transmission ratio in accordance with the increase of crack depth was identified before repairing. Furthermore, the spectral energy transmission ratio increased after the crack-repairing process in all specimens. The spectral energy transmission ratio is considered as a great indicator for estimating the crack-repairing performance of the epoxy injection method; the ratio was recovered up to almost 95% of the uncracked condition.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.1-6
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• 2007

Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss $8{\sim}10%$, coir dust $66{\sim}70%$, zeolite $13{\sim}17%$, vermiculite $3{\sim}7%$, perlite $2{\sim}4%$) and grown well into whole plants with multiple shoots.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.266-272
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• 2018

A Polygonatum stenophyllum Maxim. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of P. stenophyllum through plant regeneration from rhizome (1-year, 3-years, and 5-years) explant-derived calli. The rhizome segments were cultured in Murashige and Skoog (MS) medium supplemented with varying concentrations of 2,4-D (0, 0.5, 1.0, $1.5mg{\cdot}L^{-1}$) for callus induction. In media supplemented with $0.5mg{\cdot}L^{-1}$ of 2,4-D, 87% of 3-years rhizome produced callus. Subsequently, the callus was transferred to 1/2MS medium supplemented with various concentrations of IAA, IBA, NAA, and 2,4-D (0, 0.1, 0.5 and $1.0mg{\cdot}L^{-1}$) for adventitious shoot formation. The highest percentage of adventitious shoot induction (57%) was observed in 1/2MS medium containing $0.5mg{\cdot}L^{-1}$ of NAA. Elongation of the adventitious shoot was achieved in 1/2MS medium supplemented with $0.1mg{\cdot}L^{-1}$ of BA. Rooting was achieved in 1/2MS medium without any hormones. It is hypothesized that the stated in vitro propagation protocol will be useful for conservation and mass propagation of the endangered Polygonatum stenophyllum Maxim. for bioresources.