• Title/Summary/Keyword: 무균미니돼지

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Establishment of Hysterectomy for Gnotobiotic Pig Production (무균돼지 생산을 위한 자궁적출술 확립)

  • Nho, W.G.;Lee, J.H.;Kim, W.Y.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.10 no.1
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    • pp.91-99
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    • 2008

  • Gnotobiotic piglets were routinely produced by hysterectomy. In this study, 22 pregnant miniature pigs (111th to 113th day of gestation) were used for hysterectomy. Before surgery, 14 pigs were insensibilizated by Ketamine 50® plus CO2 gas and 8 pigs by a slaughter pistol. The high level of Ketamine 50® (0.09㎖/kg) was faster (146 vs 283 seconds) in surgery but the time taken for complete revival of one piglet was more prolonged (427 vs 64 seconds) than 0.03㎖/kg level. In hysterectomies with a slaughter pistol, surgery time was faster (470 vs 155 seconds) and the rate of alive piglets was higher (97.0 vs 83.8%) than in those with Ketamine 50®. There were no problems in the rate of alive newborn piglets even when sows were hysterectomized at 3 days prepartum.

  • https://doi.org/10.23097/JPAF:2008.10(1).91 인용 PDF

Detection and Classification of Porcine Endogenous Retroviruses by Polymerase Chain Reaction (중합효소 연쇄반응을 이용한 돼지 내인성 레트로 바이러스의 검출과 분류)

  • Lee, D.H.;Lee, J.E.;Kim, H.M.;Kim, G.W.;Park, H.Y.;Kim, Young-Bong
    • Journal of Animal Science and Technology
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    • v.49 no.3
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    • pp.405-414
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    • 2007

  • Pigs have been considered as an ideal source of donor organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human in xenotransplantation. However, for the public health risks associated with the potential for porcine endogenous retrovirus(PERV) infection through xenograft from pig to human, the investigation of methods for elimination and/or control of PERV has been required. In this study we developed the detection and classification methods for PERV based on PCR using specific primers. PERV-A and PERV-B were found in all pigs including Berkshire, Duroc, Landrace, Yorkshire, miniature pig, and Korean native black pig from Jeju by PCR with type-specific primers for PERV. However, PERV-C was detected only from Duroc, miniature pig, and Korean native black pig from Jeju. PERV-A and PERV-B could be distinguished by PCR-RFLP with BamHI. These methods for PERV will be useful in rapid screening of safe organ for xenograft, furthermore, helpful in monitoring of PERV during and after xenotransplantation.

  • https://doi.org/10.5187/JAST.2007.49.3.405 인용 PDF KSCI