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http://dx.doi.org/10.5187/JAST.2007.49.3.405

Detection and Classification of Porcine Endogenous Retroviruses by Polymerase Chain Reaction  

Lee, D.H. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
Lee, J.E. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
Kim, H.M. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
Kim, G.W. (Department of Animal Resources Science, College of Industrial Science, Kongju National University)
Park, H.Y. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
Kim, Young-Bong (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
Publication Information
Journal of Animal Science and Technology / v.49, no.3, 2007 , pp. 405-414 More about this Journal
Abstract
Pigs have been considered as an ideal source of donor organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human in xenotransplantation. However, for the public health risks associated with the potential for porcine endogenous retrovirus(PERV) infection through xenograft from pig to human, the investigation of methods for elimination and/or control of PERV has been required. In this study we developed the detection and classification methods for PERV based on PCR using specific primers. PERV-A and PERV-B were found in all pigs including Berkshire, Duroc, Landrace, Yorkshire, miniature pig, and Korean native black pig from Jeju by PCR with type-specific primers for PERV. However, PERV-C was detected only from Duroc, miniature pig, and Korean native black pig from Jeju. PERV-A and PERV-B could be distinguished by PCR-RFLP with BamHI. These methods for PERV will be useful in rapid screening of safe organ for xenograft, furthermore, helpful in monitoring of PERV during and after xenotransplantation.
Keywords
Porcine endogenous retrovirus; Xenotransplantation; Detection; Classification; PCR; RFLP;
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