• Journal of Mushroom
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• v.22 no.3
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• pp.102-106
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• 2024

This study aimed to analyze crude protein, amino acid and bioactive changes in Phellinus linteus HN00K9 cultured oat. The crude protein content of P. linteus cultured oat (PCO) was 12.9%, which was higher than that of uncultured oats (UCO) as control at 11.26%. The total free amino acid contents of PCO and UCO were 11,4 mg/100 g and 9,686.205 mg/100 g, respectively. Glutamic acid accounted for 21% of the total amino acids of PCO, and the histidine content was increased by more than 51% in PCO compared to UCO. The total polyphenol content of PCO was 275 mg GAE/g, which was more than twice that of UCO (135 mg). The DPPH radical scavenging activity was 15.5% in PCO, which was more than five times that of UCO (3.5%). The β-glucan content of PCO was 12.5 g/100 g, which was more than five times that of UCO (3.2 g/100 g). Therefore, it is believed that PCO can be utilized as a material for various functional foods.

• Journal of the Korean Wood Science and Technology
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• v.49 no.4
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• pp.336-359
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• 2021

In this study, hybrid CLT research and development trends were analyzed to improve the low rolling shear strength of CLT, a large wooden panel used in high-rise wooden buildings. Through this, basic data that can be used in research and development directions for localization of CLT were prepared. As a way to improve the low rolling shear strength, the use of hardwood lamina, the change of the lamina arrangement angle, and the use of structural composite materials are mainly used. Rolling shear strength and shear modulus of hardwood lamina are more than twice as high as softwood lamina. It confirmed that hardwoods can be used and unused species can be used. Rolling shear strength 1.5 times, shear modulus 8.3 times, bending stiffness 4.1 times improved according to the change of the layer arrangement angle, and the CLT strength was confirmed by reducing the layer arrangement angle. Structural wood-based materials have been improved by up to 1.35 times MOR, 1.5 times MOE, and 1.59 times rolling shear strength when used as laminas. Block shear strength between the layer materials was also secured by 7.0 N/mm², which is the standard for block shear strength. Through the results of previous studies, it was confirmed that the strength performance was improved when a structural wood based materials having a flexural performance of MOE 7.0 GPa and MOR 40.0 MPa or more was used. This was determined based on the strength of layered materials in structural wood-based materials. The optimal method for improving rolling shear strength is judged to be the most advantageous application of structural wood based materials with strength values according to existing specifications. However, additional research is needed on the orientation of CLT lamina arrangement according to the fiber arrangement of structural wood-based materials, and the block shear strength between lamina materials.

• Korean Journal of Microbiology
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• v.51 no.3
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• pp.209-220
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• 2015

In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genes for their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units (OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominant phyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ${\gamma}-Proteobacteria$ (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis $BCT-7112^T$ and Lactococcus lactis subsp. hordniae $NCDO\;2181^T$, respectively. Cellulolytic enzyme activity analysis showed that ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$ and ${\beta}-1,4-xylanase$ were higher in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications.

• Journal of the Korean Wood Science and Technology
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• v.45 no.3
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• pp.317-326
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• 2017

The wood and bark of Hawthorn tree (Crataegus pinnatifida Bunge) were immersed with 70% aqueous acetone for 3 days. After filtering, the wood extracts were fractionated with n-hexane, chloroform ($CHCl_3$), n-butanol (n-BuOH) and $H_2O$, and the bark extracts were fractionated with n-hexane, $CHCl_3$, ethylacetate (EtOAc) and $H_2O$. Then antioxidative and anti-inflammatory activities were evaluated on each fraction. Antioxidative activity indicated high activity in the n-butanol soluble fraction of wood and in the EtOAc soluble fraction of bark. Especially, the EtOAc soluble fraction of bark showed much higher antioxidative value compared to the controls, buthylated hydroxytoluene (BHT) and ${\alpha}$-tocopherol. In the anti-inflammatory activity, all of the tested fractions except the $H_2O$ soluble fraction of wood showed high inhibitory effect on nitric oxide (NO) production. Based on the above results, the extracts of hawthorn tree may be applied for one of the natural biomass sources that can be used as an antioxidant and an anti-inflammatory substance.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.588-598
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• 2017

Currently, bioethanol, a fuel additive for transportation, is produced mainly by using biomass (first generation) such as corn and sugar canes. First generation biomass can cause various problems in terms of increase in agricultural prices and ethical reasons. To address these problems, a nonedible lignocellulosic biomass can be utilized. Agricultural byproducts such as straw, bagasse, and forest byproducts from the wood processing industry. Therefore, production of wood based bioethanol can be an effective utilization route of second generation biomass, and its raw materials are more abundant than first generation resources. Furthermore, it is possible to secure cheap raw materials. One of the biggest advantages of using biofuels is that it contributes to the reduction of greenhouse gases by minimizing the environmental impact, unlike fossil fuels. In this study, we investigated the greenhouse gas reduction effects that can be achieved through the use of Lignocellulosic bioethanol and government policies on renewable energy currently being implemented in ASEAN countries (Indonesia, Malaysia, Thailand and the Philippines). In these four countries, policies and incentives related to biofuels have been developed. It is expected that the reduction ratio of carbon dioxide emission and the mixed biofuel will be gradually increased in the future.

• Journal of the Korean Wood Science and Technology
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• v.45 no.5
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• pp.535-543
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• 2017

For column-beam gussets of wooden structures, slit-processed members inserted with a steel plate are used in general. In this study, a rigid portal frame bonded with a joint was fabricated and a semi-rigid portal frame was fabricated by making a wooden gusset, a replacement for steel plate, of which a half was integrated into the column member and the other half was joined with the beam member by drift-pins. The lateral strength performance of the wooden portal frame was compared with that of the steel plate-inserted joint portal frame. The lateral strength performance was evaluated through a perfect elasto-plasticity model analysis, sectional stiffness change rate, and short-term permissible shear strength. As a result of the experiment, the maximum strength of the rigid portal frame was lower than that of the steel plate-inserted joint portal frame. The yield strength and ultimate strength were calculated as 0.58 and 0.48, respectively, but the measurements of initial stiffness and cumulative ductility improved by 1.35 and 1.1, respectively. As a result of the perfect elasto-plasticity model analysis of the semi-rigid portal frame, the maximum strength was lower than that of the rigid portal frame, but the toughness after failure was excellent. Thus, the ultimate strength was higher by 1.05~1.07. The steel plate-inserted portal frame showed rapid decrease in stiffness with the progress of repeated tests, but the stiffness of the portal frames with a wooden joint decreased slowly.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.2
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• pp.25-35
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• 1997

오늘날 열대림 면적의 급격한 감소, 지구온난화등 지구환경 보전의 관점에서 이들 목재이외의 자원의 활용 및 양마와 같은 새로운 비목재자원 개발의 중요성이 재인식되기에 이르렀으며, 이 가운데서 양마는 생장이 라르고 수확량이 크며, 목질부의 단섬유는 물론 긴 섬유장을 가진 인피부섬유로 구성되어 있고, 실리카함량도 매우 적어, 특히 산림자원이 부족한 개발도상국에서 펄프.제지용 자원으로서 주목되고 있다. 양마가펄프.제지용자원으로서 오늘날과 같은 세계적 주목을 받게 된 것은 미국 농무성 북부지역연구센타 에서 이루어진 “새로운 섬유작물”에 관한 기초연구에서 양마가 제지용 섬유작물로서 높이 평가되고 있다. 양마의 경우 인피부는 비교적 리그닌함량이 적고, 목질부는 활엽수재에 가까운 리그닌을 함유하고 있는데다, 섬유형태에 있어서도 인피부와 목질부간에 큰 차이가 발견된다. 본 연구에서는 양마를 중성아황산법으로 전처리하여 제조한 반화학펄프로 부터 제조한 종이의 광학적 성질을 검토하였다 펄프의 광학적 성질은 원료의 화학적 조성,섬유의 형태, 부위.화학적 처리정도, 원료의 저장기간 및 충전제의 첨가에 따라 크게 영향을 받았다. 장섬유와 낮은 리그닌함량을 가진 전간부 펄프시트는 불투명도가 낮았으며, 목질부 펄프로부터 상대적으로 밀도가 다소 높고, 불투명도도 높은 종이를 만들 수 있었다. 펄프원료의 저장기간이 백색도를 낮추었으며, 불투명도에는 그다지 영향하지 않았다. 약품전처리 및 표백이 백색도 및 불투명도에 크게 영향하였고, 특히 충전제 첨가로 광산란을 증가시켜 불투명도가 높은 종이를 제조할 수 있었다.6. CL종의 난황콜레스테롤 수준에 대한 계란의 일령 상호간에는 전체적으로 상호간에 차이가 없었다. 7. 26주령시 혈중콜레스테롤 수준은 CL종이 다른 두 육계종들 보다 유의적 (P$\leq$0.05)으로 높았다. 8. 성별간 혈중콜레스테롤 수준의 차이는 CL종, BR1 및 BR2 중에서 CL종에서만 나타났으며, 수컷은 암킷보다 혈액 100 mL당 약 90 mg이 낮았다.구체적인 생화학적인 연구가 이루어져야 될 것으로 여겨진다.야 할 것으로 사료된다. 냉장 저장을 하고 저장기간이 길어질 경우는 냉동저장을 사용하는 것이 바람직하다고 생각된다.S, FOS나 말티톨로 대체된 스폰지 케익은 설탕 100% 사용한 케익과 비교하여 크게 나쁘지 않거나 휠씬 나은 특성을 보이므로 스폰지 케익 제조에 그들의 이용이 가능함을 확인하였다.thione 같은 다른 항산화제의 절약 효과, CYP2El의 유도 저하를 통하는 것으로 생각되며 그중 어떤 것에 가장 큰 영향을 받는 지는 아직 알려지지 않았고 더욱 많은 연구가 요구된다.우와 사료에 $\omega$3계 지방산 급원을 첨가, 동물에게 이행시켜 $\omega$3계 지방산 강화식품을 제조하는 두 가지 방법이 있을 수 있는데 전자는 통조림, 과자, 건강보조식품류에 다용되며 후자는 다양한 기능성 축산식품에 활용되고 있다. $\omega$3계 지방산 강화 축산식품에는 돼지고기, 우유, 치이즈, 계란, 분유, 햄 등 다양한 제품에 활용, 개발되고 있으며 최근에는 이러한 식품 개발에 더욱 박차가

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.726-735
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• 2000

This study was conducted to investigate the biological activities of polysaccharide extracted from the fruit body and cultured mycelia of Phellinus linteus IY001. All fractions were extracted by hot water, in the next, Fr. I, Fr. II, Fr. III and Fr. IV were polysaccharide obtained by ethanol precipitation or ultrafiltration. The highest antitumor activity against sarcoma 180 in ICR mice was observed in Fr. III and Fr. IV at the level 85%, but the antitumor activity had no connection with their anticomplementary activity in vitro, it might probably be due to extraction of hot water. All fractions promoted the production of nitric oxide and $TNF-{\alpha}$ in macrophage, addition of Fr. I and Fr. II resulted in production of nitric oxide$3(5.9{\sim}37.6\;{\mu}M)$ and of the $TNF-{\alpha}$ production($8,696.2{\sim}9,420pg/ml)$. All fractions inhibited the lipid peroxidation induced by $AsA/Fe^{2+}$, $ADP/NADPH/Fe^{3+}\;and\;CCl_4/NADPH$ in rat liver microsomes, and Fr. III showed the electron donating ability stronger than tocopherol in assay system using DPPH. From these results, it is suggested that all fractions contain immunoregulatory components which may protect cellular materials from the oxidative damages by their radical scavenging activities.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.704-711
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• 2010

Lignocellulose ($2^{nd}$ generation) is difficult to hydrolyze due to the presence of lignin and the technology developed for cellulose fermentation to ethanol is not yet economically viable. However, recent advances in the extremely new field of biotechnology for the ethanol production are making it possible to use of agriculture residuals and nonedible crops biomass, e.q., rice straw and miscanthus sinensis, because of their several superior aspects as agriculture residual and nonedible crops biomass; low lignin, high contents of carbohydrates. In this article, as the basic study of AP(Ammonia Percolation), the properties and the optium conditions of process were established, and then the overall efficiency of AP was investigated. The important independent variables for AP process were selected as ammonia concentration, reaction temperature, and reaction time. The percolation condition for maximizing the content of cellulose, the enzymatic digestibility, and the lignin removal was optimized using RSM(Response Surface Methodology). The determined optimum condition is ammonia concentration; 11.27%, reaction temperature; $157.75^{\circ}C$, and reaction time; 10.01 min. The satisfying results were obtained under this optimized condition, that is, the results are as follows: cellulose content(relative); 39.98%, lignin content(relative); 8.01%, and enzymatic digestibility; 85.89%.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.239-246
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• 2015

Two fungal strains were isolated from rods of Quercus sp. (NAAS02335) and Pinus densiflora (NAAS05299) in Korea. These strains were identified as Gyrodontium sacchari by their morphological and mycological characteristics. The optimal growth temperature of NAAS02335 and NAAS05299 are $25^{\circ}C$ and $30^{\circ}C$, respectively. Production of cellulase, xylanase, and ligninase was tested on agar media supplemented dyes or substrates. Production of cellulase and xylanase of NAAS05299 was higher than those of NAAS02335, however ligninase activity of NAAS02335 was higher than that of NAAS05299. The activities of cellulase, xylanase, and amylase of strain NAAS05299 were estimated at 6.7~10.2 times higher than that of NAAS02335. Laccase activity was only estimated by strain NAAS02335. The lignocellulytic enzymes are induced by substrates such as rice straw, wooden chips of pine, oak, and poplar. The NAAS05299 was able to degrade filter paper completely after 4 weeks of culturing in liquid media containing a piece of filter paper at $28^{\circ}C$ with continuous shaking. NAAS05299 was able to degrade rice straw, pine chips, and oak chips after 4 months in solid culture, however NAAS02335 decomposed only rice straw among tested 4 kinds of biomass.